Effect of the Surface Charge-Dependent Boundary Slip on the Electrophoresis of a Hydrophobic Polarizable Rigid Colloid.

The electrophoresis of a hydrophobic charged rigid colloid is studied by considering the lateral movement of the adsorbed surface charge. The slip velocity condition at the hydrophobic surface is modified to take into account the impact of the frictional and electric forces created by the adsorbed laterally mobile surface charge. Though the dependency of the surface charge on the slip velocity in the context of electrophoresis has been addressed before, the effect of the laterally mobile adsorbed surface charge on the electrophoresis of hydrophobic colloids has not been studied. The dielectric colloid is considered to polarize and create an induced immobile surface charge when subjected to an imposed electric field. The impact of the mobile surface charge along with the immobile induced surface charge on electrophoresis of a hydrophobic colloid is elucidated by numerically solving the governing electrokinetic equations in their full form. We have also developed a simplified model under a weak applied field consideration, which can be further reduced to a closed-form analytic expression for the mobility under the Debye-Hückel approximation. This analytic model for mobility is in excellent agreement with the exact numerical solution for an entire range of the Debye length when the ζ-potential is in the order of the thermal potential. One of the notable features of this closed-form mobility expression is that it accounts for the mobile adsorbed surface charge on the hydrodynamic slip condition and the dielectric polarization of the particle. We find that the mobility of the surface charge decreases the electrophoretic mobility of the hydrophobic dielectric colloid. However, the mobile surface charge enhances the mobility of a conducting hydrophobic colloid.

Numerical study supplemented with simplified model on electrophoresis of a hydrophobic colloid incorporating finite ion size effects and ion-solvent interactions.

We consider a modified electrokinetic model to study the electrophoresis of a hydrophobic particle by considering the finite sized ions. The mathematical model adopted in this study incorporates the ion steric repulsion, ion-solvent interactions as well as Maxwell stress on the electrolyte. The dielectric permittivity and viscosity of the electrolyte is considered to vary with the local ionic volume fraction. Based on this modified model for the electrokinetics we have analyzed the electrophoresis in a single as well as mixture of electrolytes of monovalent and non- z : z $z:z$ electrolytes. The dependence of viscosity on local ionic volume fraction modifies the hydrodynamic drag as well as diffusivity of ions, which are ignored in existing studies on electrophoresis. A simplified model for electrophoresis of a hydrophobic particle incorporating the ion steric repulsion and ion-solvent interactions is developed based on the first-order perturbation on applied electric field. This simplified model is established to be efficient for a Debye layer thinner than the particle size and a smaller range of slip length. This model can be implemented for any number of ionic species as well as non- z : z $z:z$ electrolytes. It is established that the ion steric interactions and dielectric decrement creates a counterion saturation in the Debye layer leading to an enhanced mobility compared to the standard model. However, experimental data for non-dilute cases often under predicts the theoretically determined mobility. The present modified model fills this lacuna and demonstrate that the consideration of finite ion size modifies the medium viscosity and hence, ionic mobility, which in combination lowers the mobility value.

Diffusiophoresis of a Charged Droplet in Asymmetric as Well as Mixed Electrolytes through Numerical and Semianalytic Models.

A numerical study on the diffusiophoresis of a droplet in an electrolyte medium is carried out by solving the full set of coupled governing equations, which are based on the conservation principle. Diffusiophoresis is considered for monovalent as well as non-z:z electrolytes and mixed electrolytes. The numerical model is supplemented with a semianalytic simplified model based on first-order perturbation analysis, which agrees with the numerical model for a low to moderate range of surface potential. The mobility for a low-viscosity fluid at a thinner Debye length is dominated by the chemiphoresis part, which creates the mobility to become an even function of the surface charge density for a monovalent electrolyte. Such a pattern in mobility does not appear in a non-z:z asymmetric electrolyte. At a thinner Debye length, diffusiophoresis becomes independent of the diffusion field, hence the mobility is independent of the composition of electrolytes in a mixed monovalent electrolyte solution. Our results show that the size-based sorting of droplets is efficient when a mixed electrolyte is considered. We have also addressed the finite ion size effects by considering a modified ion transport equation. One of the key features of the present study is the simplified semianalytical model for the diffusiophoresis of a droplet in a z:z electrolyte as well as in non-z:z and mixed electrolytes, which is shown to be valid up to a moderate range of surface potential for a finite Debye length.

Observation of High Magnetic Bistability in Lanthanide (Ln = Gd, Tb and Dy)-Grafted Carbon Nanotube Hybrid Molecular System.

There is an immense research interest in molecular hybrid materials posing novel magnetic properties for usage in spintronic devices and quantum technological applications. Although grafting magnetic molecules onto carbon nanotubes (CNTs) is nontrivial, there is a need to explore their single molecule magnetic (SMM) properties post-grafting to a greater degree. Here, we report a one-step chemical approach for lanthanide-EDTA (Ln = GdIII, 1; TbIII, 2 and DyIII, 3) chelate synthesis and their effective grafting onto MWCNT surfaces with high magnetic bistability retention. The magnetic anisotropy of an Ln-CNT hybrid molecular system by replacing the central ions in the hybrid complex was studied and it was found that system 1 exhibited a magnetization reversal from positive to negative values at 70 K with quasi-anti-ferromagnetic ordering, 2 showed diamagnetism to quasi-ferromagnetism and 3 displayed anti-ferromagnetic ordering as the temperature was lowered at an applied field of 200 Oe. A further analysis of magnetization (M) vs. field (H) revealed 1 displaying superparamagnetic behavior, and 2 and 3 displaying smooth hysteresis loops with zero-field slow magnetic relaxation. The present work highlights the importance of the selection of lanthanide ions in designing SMM-CNT hybrid molecular systems with multi-functionalities for building spin valves, molecular transistors, switches, etc.

Resonant Nanopumps: ac Gate Voltages in Conical Nanopores Induce Directed Electrolyte Flow.

Inducing transport in electrolyte-filled nanopores with dc fields has led to influential applications ranging from nanosensors to DNA sequencing. Here we use the Poisson-Nernst-Planck and Navier-Stokes equations to show that unbiased ac fields can induce comparable directional flows in gated conical nanopores. This flow exclusively occurs at intermediate driving frequencies and hinges on the resonance of two competing timescales, representing space charge development at the ends and in the interior of the pore. We summarize the physics of resonant nanopumping in an analytical model that reproduces the results of numerical simulations. Our findings provide a generic route toward real-time controllable flow patterns, which might find applications in controlling the translocation of small molecules or nanocolloids.

Recent advances in the bioremediation of arsenic-contaminated soils: a mini review.

The carcinogenic metalloid arsenic (As), owing to its persistent behavior in elevated levels in soils, aggravates environmental and human health concerns. The current strategies used in the As decontamination involve several physical and chemical approaches. However, it involves high cost and even leads to secondary pollution. Therefore, it is quite imperative to explore methods that can eradicate As menace from the environment in an eco-friendly, efficient, and cost-competitive way. Searching for such viable alternatives leads to the option of bioremediation technology by utilizing various microorganisms, green plants, enzymes or even their integrated methods. This review is intended to give scientific and technical details about recent advances in the bioremediation strategies of As in soil. It takes into purview the extent, toxicological manifestations, pathways of As exposure and exemplifies the substantive need of bioremediation technologies such as phytoremediation and biosorption in a descriptive manner. Additionally, the paper looks into the wide potential of some plant growth promoting microorganisms (PGPMs) that improve plant growth on one hand and alleviate As toxicity on the other. Furthermore, it also makes a modest attempt to assimilate the use of nanoparticles, non-living biomass and transgenic crops which are the emerging alternative bioremediation technologies.

Demonstration of the Holonomically Controlled Non-Abelian Geometric Phase in a Three-Qubit System of a Nitrogen Vacancy Center.

The holonomic approach to controlling (nitrogen-vacancy) NV-center qubits provides an elegant way of theoretically devising universal quantum gates that operate on qubits via calculable microwave pulses. There is, however, a lack of simulated results from the theory of holonomic control of quantum registers with more than two qubits describing the transition between the dark states. Considering this, we have been experimenting with the IBM Quantum Experience technology to determine the capabilities of simulating holonomic control of NV-centers for three qubits describing an eight-level system that produces a non-Abelian geometric phase. The tunability of the geometric phase via the detuning frequency is demonstrated through the high fidelity (~85%) of three-qubit off-resonant holonomic gates over the on-resonant ones. The transition between the dark states shows the alignment of the gate's dark state with the qubit's initial state hence decoherence of the multi-qubit system is well-controlled through a π/3 rotation.

Electrokinetic actuation of an uncharged polarizable dielectric droplet in charged hydrogel medium.

Electrokinetic transport of an uncharged nonconducting microsized liquid droplet in a charged hydrogel medium is studied. Dielectric polarization of the liquid drop under the action of an externally imposed electric field induces a non-homogeneous charge density at the droplet surface. The interactions of the induced surface charge of the droplet with the immobile charges of the hydrogel medium generates an electric force to the droplet, which actuates the drop through the charged hydrogel medium. A numerical study based on the first principle of electrokinetics is adopted. Dependence of the droplet velocity on its dielectric permittivity, bulk ionic concentration, and immobile charge density of the gel is analyzed. The surface conduction is significant in presence of charged gel, which creates a concentration polarization. The impact of the counterion saturation in the Debye layer due to the dielectric decrement of the medium is addressed. The modified Nernst-Planck equation for ion transport and the Poisson equation for the electric field is considered to take into account the dielectric polarization. A quadrupolar vortex around the uncharged droplet is observed when the gel medium is considered to be uncharged, which is similar to the induced charge electroosmosis around an uncharged dielectric colloid in free-solution. We find that the induced charge electrokinetic mechanism creates a strong recirculation of liquid within the droplet and the translational velocity of the droplet strongly depends on its size for the dielectric droplet embedded in a charged gel medium.

Settling of a charged hydrophobic rigid colloid in aqueous media under generalized gravitational field.

The hindrance created by the induced electric filed on the sedimentation of a charged colloid in an aqueous media is studied through numerical modeling. The colloid is considered to be hydrophobic, sedimenting under gravity or a centrifugal force (generalized gravity). The deformation of the charge cloud around the colloid induces an electric field, which generates electrical dipole force on the colloid. The sedimentation velocity is governed by the balance of an electric force, hydrodynamic drag, and gravitational force. Governing equations based on the first principle of electrokinetics is solved numerically through a control volume approach. The dependence of the sedimentation velocity on the electrical properties and slip length of the colloid is investigated. The sedimentation velocity of the charged colloid is slower than the corresponding uncharged particle and this deviation magnifies as the charge density as well as particle slip length is increased. An enhanced g-factor creates a size dependency of the charged colloids. The induced sedimentation field is obtained to analyze the electrokinetics. Surface hydrophobicity enhances the sedimentation velocity, which in turn manifests the induced sedimentation field. However, the sedimentation velocity of a charged hydrophobic colloid is lower than the corresponding uncharged hydrophobic particle and this deviation manifests as slip length is increased.

Investigation of arsenic-resistant, arsenite-oxidizing bacteria for plant growth promoting traits isolated from arsenic contaminated soils.

The problem of arsenic (As) pollution being severe warrants opting for low-cost microbial remediation strategies. The present study of identifying suitable bacterial strains led to the isolation of eleven As-tolerant strains from the As-contaminated rhizosphere soils of West Bengal, India. They were found to oxidize/reduce 55-31.6% of 5 mM As(III) and 73-37.6% of 5 mM As(V) within 12 h. The four isolates (BcAl-1, JN 73, LAR-2, and AR-30) had a high level of As(III) oxidase activity along with a higher level of As(V) and As(III) resistance. The agar diffusion assay of the isolates further confirmed their ability to endure As stress. The presence of aoxB gene was observed in these four As(III) oxidizing isolates. Evaluation of plant growth-promoting characteristics revealed that BcAl-1 (Burkholderia cepacia), JN 73 (Burkholderia metallica), AR-30 (Burkholderia cenocepacia), and LAR-2 (Burkholderia sp.) had significant plant growth-promoting characteristics (PGP), including the ability to solubilize phosphate, siderophore production, indole acetic acid-like molecules production, ACC deaminase production, and nodule formation under As stressed condition. BcAl-1 and JN 73 emerged as the most promising traits in As removal as well as plant growth promotion.

Charge Selectivity of an Ionic Transistor.

The charge selective properties of a long planar nanochannel with an embedded finite uniformly charged section in the middle are studied. The probability flux of a single test ion initially confined to the inlet reservoir is determined by integrating the Smoluchowski equation using a previously published series solution for the Debye-Hückel potential in this geometry. The charge selective properties are characterized by a dimensionless quantity that we call the "fractional blockage". We study how the fractional blockage depends on the dimensionless parameters that characterize the charge state and channel geometry. In the limit of strongly overlapped wall Debye layers, analytical expressions for the fractional blockage are presented that are found to be in good agreement with numerically computed values in the appropriate asymptotic regimes. These results may be helpful in the design of nanofluidic devices that have a variety of applications.

Electromagnetic Irradiation Evokes Physiological and Molecular Alterations in Rice.

Electromagnetic energy is the "backbone" of wireless communication systems, and its progressive use is considered to have a low but measurable impact on a wide range of biological systems. Even though a growing amount of data has reported electromagnetic energy absorption in humans along with subsequent biological effects, the consequences of electromagnetic energy absorption on plants have been insufficiently addressed. The higher surface to volume ratio along with the enormous water-ion concentrations makes the plant an ideal model to interact with non-ionizing electromagnetic radiation. In this study, controlled and periodic electromagnetic exposure of 1837.50 MHz, 2.75 W/m2 for 6 h a day on a popular rice variety (var. Satabdi) reduced the seed germination rate. The same dose of periodic electromagnetic exposure upregulated phytochrome B and phytochrome C gene transcripts in 12-day-old seedlings, whereas, in 32-day-old plants, the dose upregulated calmodulin and phytochrome C while the bZIP1 gene showed repression. However, the transcript abundance of bZIP1, phytochrome B, and phytochrome C genes was enhanced even in 12-day-old Satabdi seedlings following instantaneous short-duration (2 h 30 min) controlled electromagnetic exposure to 1837.50 MHz, 2.75 W/m2 . The reported responses in rice were observed below the international electromagnetic regulatory limits. Thus, rice plants perceived electromagnetic energy emitted by the wireless communication system as abiotic stress as per its response by upregulation or repression of known stress-sensing genes. Bioelectromagnetics. © 2020 Bioelectromagnetics Society.

One-time Electromagnetic Irradiation Modifies Stress-sensitive Gene Expressions in Rice Plant.

Electromagnetic energy is utilized over multiple frequency bands to provide seamless wireless communication services. Plants can well perceive electromagnetic energy present in open environment due to reasonably high permittivity and electrical conductivity of constituent tissues. Moreover, higher surface-to-volume ratio of plant structure facilitates increased interaction with the incident electromagnetic waves. To date, a few well-designed studies have been conducted inside controlled electromagnetic reverberation chambers to investigate either short duration-low amplitude or long duration-periodic electromagnetic irradiation-induced molecular responses in plants. However, as far as is known, studies investigating molecular responses particularly at the mid-vegetative stage in plants following one-time (hours-long) electromagnetic irradiation have not been reported earlier. Hence, the present study aimed at investigating molecular responses in 40-day-old Swarnaprabha rice plants following one-time 1837.50 MHz, 2.75 mW/m2 electromagnetic irradiation of 2 h 30 min duration. Controlled electromagnetic irradiation inside a simple reverberation chamber was ensured to achieve pure electromagnetic environment at 1837.50 MHz with deterministic electromagnetic power density at selected position. Swarnaprabha rice plant was chosen for this investigation since the rice variety is widely cultivated and consumed in the Indian subcontinent. Subsequent alterations in some selected stress-sensitive gene expressions were assayed using real-time quantitative polymerase chain reaction technique-significant upregulation in calmodulin and phytochrome B gene expressions were noted. This investigation was purposefully focused on subsequent molecular responses immediately following electromagnetic irradiation so that the possible effects of secondary stimulations could be avoided. Observed molecular responses strongly suggested that plants perceive 1837.50 MHz, 2.75 mW/m2 electromagnetic irradiation similar to other injurious stimuli. © 2021 Bioelectromagnetics Society.

A perfect PCR based co-dominant marker for low grain-arsenic accumulation genotyping in rice.

The development of low arsenic-accumulating varieties for the contaminated areas is one of the best options for reducing the dietary exposure of arsenic to human population through rice. In this study, grain-arsenic content in one hundred genotypes revealed a large variation ranging from 0.05 mg/kg to 0.49 mg/kg. Compared to high accumulating variety, Shatabdi, 6-8 times the transcript upregulation of Arsenic sequestering ATP binding cassette C1 type gene (ABCC1), was observed in first internode of low accumulating variety Gobindabhog when 5 mg/kg of arsenite was present in soil. A comparison of the genomic sequence of OsABCC1 identified 8 SNPs between the two genotypes; 5 in introns and 3 silent mutations in exons. We identified a PCR based co-dominant marker targeting an SNP (T/G) between the two genotypes, which clearly distinguished 100 genotypes into low (mean 0.14 mg/kg) and high (mean 0.35 mg/kg) accumulating groups. All aromatic genotypes, either long or small grain, carry the Gobindabhog-type ABCC1 allele and are low accumulators of arsenic. Gobindabhog allele carrying 62 RILs and NILs showed almost 40-50% less As-accumulation in grains relative to 84 RILs and NILs carrying Shatabdi type ABCC1-allele. The marker will be useful in introgression of low accumulating allele of OsABCC1 into high yielding photoperiod insensitive varietal backgrounds more easily and accurately.

Tuning Magnetic Properties of a Carbon Nanotube-Lanthanide Hybrid Molecular Complex through Controlled Functionalization.

Molecular magnets attached to carbon nanotubes (CNT) are being studied as potential candidates for developing spintronic and quantum technologies. However, the functionalization routes used to develop these hybrid systems can drastically affect their respective physiochemical properties. Due to the complexity of this systems, little work has been directed at establishing the correlation between the degree of functionalization and the magnetic character. Here, we demonstrate the chemical functionalization degree associated with molecular magnet loading can be utilized for controlled tuning the magnetic properties of a CNT-lanthanide hybrid complex. CNT functionalization degree was evaluated by interpreting minor Raman phonon modes in relation to the controlled reaction conditions. These findings were exploited in attaching a rare-earth-based molecular magnet (Gd-DTPA) to the CNTs. Inductively coupled plasma mass spectrometry, time-of-flight secondary ion mass spectrometry and super conducting quantum interference device (SQUID) measurements were used to elucidate the variation of magnetic character across the samples. This controlled Gd-DTPA loading on the CNT surface has led to a significant change in the nanotube intrinsic diamagnetism, showing antiferromagnetic coupling with increase in the Weiss temperature with respect to increased loading. This indicates that synthesis of a highly correlated spin system for developing novel spintronic technologies can be realized through a carbon-based hybrid material.

Analytic Expression for Electrophoretic Mobility of Soft Particles with a Hydrophobic Inner Core at Different Electrostatic Conditions.

This paper presents a simplified model for the electrophoresis of a soft particle with a nonwettable rigid core with charged polyelectrolyte corona under a weak-field and low-charge density consideration. We have derived a closed form solution for the mobility, which reduces to the well-known expressions for mobility as derived by Ohshima for limiting cases such as a hydrophilic charged core coated with an uncharged polymer (Ohshima, H. J. Colloid Interface Sci. 2002, 252, 119-125) or an uncharged no-slip core coated with a polyelectrolyte layer (Ohshima, H. Electrophoresis 2006, 27, 526-533). The generalized mobility expression reduces to the existing expression for mobility of a rigid hydrophobic colloid as the soft layer shrinks to zero. The general form of the mobility expression involves elliptic integrals, which can be computed easily through a software like Mathematica. We have derived analytical solutions for mobility pertaining to several particular cases. The occurrence of mobility reversal when the core and polyelectrolyte layer has a charge of opposite polarity is demonstrated in this paper.

Influence of slip velocity at the core of a diffuse soft particle and ion partition effects on mobility.

Nonlinear effects on the electrophoresis of a soft particle, consisting of a rigid hydrophobic core coated with a diffuse polymer layer (PEL) suspended in an electrolyte medium, are studied. The impact of the ion partitioning effect arising due to the Born energy difference between the PEL and the electrolyte is approximated based on the equilibrium Boltzmann equation, with which the ion distribution and hence, the charge density is modified. The equations describing the electrokinetic transport comprising the Darcy-Brinkman extended Navier-Stokes equations which includes the ion partitioning effect coupled with the modified Nernst-Planck equations and Poisson equations for electric field are solved numerically. The present numerical model for the soft particle compares well with the existing theoretical solutions and experimental results in the limiting cases. A deviation from existing simplified models based on the Boltzmann distribution of ions occurs when the Debye layer polarization, relaxation and the electroosmosis induced by the PEL immobile charge become significant. The hydrophobicity of the inner core strongly influences the nonlinear electrokinetic effects by modifying the Debye layer, electroosmotic flow in the PEL and surface conduction. The results indicate that the ion partitioning can significantly increase the electrophoretic mobility of the soft particle by attenuating the shielding effect. When the Debye layer is in the order of the particle size the hydrophobicity of the core surface and the ion partitioning effect manifest the surface conduction, which implies that the Boltzmann distribution of ions is no longer valid. The core hydrophobicity and ion partitioning effect have influence on the condensation of the PEL immobile charge, which creates a significant impact on the mobility.

Biotechnological advancements in Catharanthus roseus (L.) G. Don.

Catharanthus roseus (L.) G. Don, also known as Madagascar periwinkle or Sadabahar, is a herbaceous plant belonging to the family Apocynaceae. Being a reservoir for more than 200 alkaloids, it reserves a place for itself in the list of important medicinal plants. Secondary metabolites are present in its leaves (e.g., vindoline, vinblastine, catharanthine, and vincristine) as well as basal stem and roots (e.g., ajmalicine, reserpine, serpentine, horhammericine, tabersonine, leurosine, catharanthine, lochnerine, and vindoline). Two of its alkaloids, vincristine and vinblastine (possessing anticancerous properties), are being used copiously in pharmaceutical industries. Till date, arrays of reports are available on in vitro biotechnological improvements of C. roseus. The present review article concentrates chiefly on various biotechnological advancements based on plant tissue culture techniques of the last three decades, for instance, regeneration via direct and indirect organogenesis, somatic embryogenesis, secondary metabolite production, synthetic seed production, clonal fidelity assessment, polyploidization, genetic transformation, and nanotechnology. It also portrays the importance of various factors influencing the success of in vitro biotechnological interventions in Catharanthus and further addresses several shortcomings that can be further explored to create a platform for upcoming innovative approaches. KEY POINTS: • C. roseus yields anticancerous vincristine and vinblastine used in pharma industry. •In vitro biotechnological interventions prompted major genetic advancements. • This review provides an insight on in vitro-based research achievements till date. • Key bottlenecks and prospective research methodologies have been identified herein.

Further observations on Meloidogyne enterolobii (Nematoda: Meloidogynidae) infecting guava (Psidium guajava) in India.

Root-knot nematodes (Meloidogyne spp.) infect a large number of crops including guava. We investigated a population of Meloidogyne sp. infecting guava in the Coimbatore region of Tamil Nadu, India for identification and species confirmation. Detailed morphological and morphometric observations based on second-stage juveniles, males, females, and perineal patterns showed resemblance of the isolated population with the original and subsequent descriptions of M. enterolobii. Isozyme analysis of the young egg-laying females displayed the characteristic esterase phenotype pattern similar to that of M. enterolobii. Additionally, the identity of the nematode population was further validated by M. enterolobii specific SCAR marker and ITS rDNA. Recently published reports on the occurrence and morphological descriptions of M. enterolobii from India are largely incongruent with the original and subsequent redescriptions of the species. Here, we present the most comprehensive morphology and morphometrics of an Indian population of M. enterolobii for its authentic identification.

On electrophoresis of a pH-regulated nanogel with ion partitioning effects.

The electrophoresis of a polyelectrolyte nanoparticle, whose charge condition depends on the salt concentration and pH of the suspended medium as well as the dielectric permittivity difference, is analyzed. The present nonlinear model for the electrophoresis of this pH-regulated polyelectrolyte (PE) particle is based on the consideration of full set of governing equations of fluid and ion transport coupled with the equation for electric field. The Born energy of the ions are incorporated to account for the difference in the dielectric permittivity of the PE and the electrolyte. The governing equations are computed numerically through a control volume approach. The nonlinear effects are highlighted by comparing with the existing linear model as well as results based on the first-order perturbation analysis valid for a weak applied field. The ion partitioning effect arising due to the difference in self energy of ions between the two media, have a strong impact on the mobility of the PE. The ion partitioning effect attenuates the penetration of counterions in the PE, which enhances the electric force and hence, results in a larger mobility of the PE. The nonlinear effects due to the double layer polarization and relaxation are intensified due to the ion partitioning effect. The ion partitioning effect influences the association/dissociation of PE functional group by tuning the hydrogen/hydroxide ions. Present study shows that the ion partitioning effect is profound for higher salt concentration and/or higher volume density of PE functional groups.