Two-stage cultivation strategies for optimal production of Ganoderma pellets with potential application in the vegan food industry.

The vegan food industry is gaining popularity nowadays. Ganoderma sp. is mainly used in the health and food industries as a medicinal, edible mushroom with high nutritional potential. Through two-stage cultivation methods, the study optimized the production of mycelial pellets for vegetarian food. When soybean powder was used as an alternative to egg yolk powder to meet vegetarian requirements, the number of pellets increased from 1100 to 1800 particles/dL, however, the pellet diameter reduced up to 22% (3.2-2.6 mm). The culture was expanded to the second stage using the Taguchi method coupled with Plackett-Burman Design and quantification by ImageJ software for enlarging pellets size. The optimal conditions were 10 mL of the first-stage broth inoculum, yeast powder (0.5 g/dL), glucose (0.5 g/dL), and MgSO4 (0.2 g/dL) at 100 rpm in the dark for 7 days. In 500 mL pilot scale production, the biomass yield was 0.31 g/dL and 3400 mycelium pellets/dL with a 5.2 mm diameter with appearance characteristics suitable for direct development as food. The study may help to develop a novel pellet food of filamentous fungi for the vegetarian market. Supplementary Information: The online version contains supplementary material available at 10.1007/s13197-023-05719-x.

Enhancing labor productivity as a key strategy for fostering green economic growth and resource efficiency.

Amidst a time when uncontrolled economic growth has frequently harmed the environment, it is crucial to reassess our strategy toward economic progress. The necessity to tackle climate change, resource depletion, and environmental deterioration demands a profound transition towards ecologically sound and sustainable economic development. This study examines the crucial significance of labor productivity in promoting sustainable economic growth and the effective utilization of resources in Asia, Europe, and South America from 1990 to 2020. To accomplish this, we utilized the Data Envelopment Analysis (DEA) methodology to examine a range of input and output characteristics thoroughly. These parameters included labor productivity, renewable energy usage, material efficiency, Green GDP, carbon footprint, and water footprint. The results of our study demonstrate significant regional variations in the efficient utilization of labor and resources to promote sustainable economic development. The findings of the DEA model emphasize that countries with higher labor productivity are more capable of pursuing an environmentally benign and sustainable financial path. Moreover, our research demonstrates a substantial association between enhanced labor productivity and diminished carbon and water footprints. This highlights the importance of labor productivity as a fundamental element for maximizing resource efficiency. In addition, we propose policy suggestions that motivate and improve worker efficiency as a practical strategy to accomplish both economic growth and environmental sustainability.

Innovative Approaches to Fungal Food Production: Mycelial Pellet Morphology Insights.

Mycelia products enhance edible mushrooms in alignment with future sustainability trends. To meet forthcoming market demands, the morphology of mycelial pellets was optimized for direct consumption. Among ten commercial edible mushrooms in Taiwan, Pleurotus sp. was selected for its rapid growth and was identified via an internal transcribed spacer sequence. A combination of Plackett-Burman design and Taguchi's L9(34) orthogonal table revealed the optimal formula as potato dextrose broth (2.4%), olive oil (2%), calcium carbonate (0.5%), yeast extract (0.75%), and soy flour (0.5%). This led to a biomass increase to 19.9 ± 1.1 g/L, resulting in a 2.17-fold yield increase. To refine morphology, image processing by ImageJ quantified spherical characteristics. The addition of 0.2 to 1.0% Tween 80 enhanced pellet compaction by over 50%. Dilution of the medium improved uniformity (0.85) and conversion rate (42%), yielding mycelial pellets with 2.10 ± 0.52 mm diameters and a yield of 15.1 ± 0.6 g/L. These findings provide an alternative evaluation and application of edible mycelial pellets as future food.

Recent advances in nanocomposite-based delivery systems for targeted CRISPR/Cas delivery and therapeutic genetic manipulation.

CRISPR/Cas systems are novel gene editing tools with tremendous capacity and accuracy for gene editing and hold great potential for therapeutic genetic manipulation. However, the lack of safe and efficient delivery methods for CRISPR/Cas and its guide RNA hinders their wide adoption for therapeutic applications. To this end, there is an increasing demand for safe, efficient, precise, and non-pathogenic delivery approaches, both in vitro and in vivo. With the convergence of nanotechnology and biomedicine, functional nanocomposites have demonstrated unparalleled sophistication to overcome the limits of CRISPR/Cas delivery. The tunability of the physicochemical properties of nanocomposites makes it very easy to conjugate them with different functional substances. The combinatorial application of diverse functional materials in the form of nanocomposites has shown excellent properties for CRISPR/Cas delivery at the target site with therapeutic potential. The recent highlights of selective organ targeting and phase I clinical trials for gene manipulation by CRISPR/Cas after delivery through LNPs are at the brink of making it to routine clinical practice. Here we summarize the recent advances in delivering CRISPR/Cas systems through nanocomposites for targeted delivery and therapeutic genome editing.

Cu-doped mesoporous TiO2 photocatalyst for efficient degradation of organic dye via visible light photocatalysis.

A solvothermal method was used to synthesize the mesoporous TiO2, (1-3w %) Cu-doped mesoporous TiO2 membrane with the help of a bioreactor. To understand the physicochemical composition of all synthesized nanomaterials, the structure, morphology and crystallinity of the materials were studied using X-ray diffractometer (XRD), Field emission scanning electron microscopy (FESEM), Fourier transform-infrared (FTIR), Energy dispersive X-ray spectroscopy (EDS) and cyclic voltammetry (CV). Under artificial light source (500 W mercury bulb) irradiations, the nano catalysts' catalytic effectiveness was examined for the azo dyes, namely Congo red. Cu-doping causes a shift in the light absorption of mTiO2 from the ultraviolet to the visible region. The 3w% Cu-doped mTiO2 photocatalyst exhibits lower band gap energy (2.6eV) than TiO2 which is 3.2 eV to efficiently utilize solar energy. As a result, the light absorption was shifted towards the visible spectrum. The recommended mTiO2 and (1, 2, 3) w% Cu-doped mTiO2 photocatalysts were used to photodegrade Congo red and methylene blue. For the degradation of CR, the mTiO2 photocatalyst exhibited 61% and 3w% Cu-doped mTiO2 demonstrated 99% photocatalytic performance after 50 min. A variety of scavengers were also utilized to distinguish the active species by catching the radicals and holes created during the process of photocatalytic degradation. CV indicates the presence of Cu2+ and Cu1+ in Cu-doped mTiO2. Oxygen vacancies and the electronegative surface of Cu1+ seem to perform the photocatalytic reduction of CR.