Morphological Evaluation and Immunohistochemical Analysis of the Reparative Potential of the Buccal Fat Pad.

Background and Objectives: There are many surgical techniques for oroantral communication treatment, one of which is the buccal fat pad. Of particular interest is the high reparative potential of the buccal fat pad, which may be contributed to by the presence of mesenchymal stem cells. The purpose of this work is to evaluate the reparative potential of BFP cells using morphological and immunohistochemical examination. Materials and Methods: 30 BFP samples were provided by the Clinic of Maxillofacial and Plastic Surgery of the Russian University of Medicine (Moscow, Russia) from 28 patients. Morphological examination of 30 BFP samples was performed at the Institute of Clinical Morphology and Digital Pathology of Sechenov University. Hematoxylin-eosin, Masson trichrome staining and immunohistochemical examination were performed to detect MSCs using primary antibodies CD133, CD44 and CD10. Results: During staining with hematoxylin-eosin and Masson's trichrome, we detected adipocytes of white adipose tissue united into lobules separated by connective tissue layers, a large number of vessels of different calibers, as well as the general capsule of BFP. The thin connective tissue layers contained neurovascular bundles. Statistical processing of the results of the IHC examination of the samples using the Mann-Whitney criterion revealed that the total number of samples in which the expression of CD44, CD10 and CD133 antigens was confirmed was statistically significantly higher than the number of samples where the expression was not detected (p < 0.05). Conclusions: During the morphological study of the BFP samples, we revealed statistically significant signs of MSCs presence (p < 0.05), including in the brown fat tissue, which proves the high reparative potential of this type of tissue and can make the BFP a choice option among other autogenous donor materials when eliminating OAC and other surgical interventions in the maxillofacial region.

Evaluation of Dentinal Thickness and Remaining Dentine Volume around Root Canals Using Cone-Beam Computed Tomography Scanning.

BACKGROUND: The remaining dentinal thickness is a significant factor to deal with when planning post-endodontic treatment. AIM: To assess the changes in the root canal dentinal thickness of intact and endodontically treated teeth using CBCT scans in the coronal, middle, and apical third of the root canal. MATERIAL AND METHODS: Three hundred CBCT scans for three age groups were analyzed to study the dentinal thickness pre- and post-endodontic treatment. The dentinal thickness (DT) was measured along the buccal, mesial, distal, and lingual/palatal walls from the inner surface of the root canal to the outer surface in mm. Statistical analysis was set at 0.05. RESULTS: The results of this study showed that the buccal, palatal, distal, and mesial dentinal thickness in intact and endodontically treated teeth is different. The differences were statistically significant when comparing the parameters of "healthy" and "treated" teeth (p < 0.05). There were no statistically significant differences in indicators associated with age (p > 0.05). In the coronal third of the root canal, the least amount of dentin tissue lost was 4.2% for mandibular canines. CONCLUSIONS: The dentinal thickness in the coronal and middle third of the root decreases significantly more than the apical third. The most loss of dentine volume was among molar teeth, and the remaining dentinal thickness was less than 1 mm, which would increase the risk of complication while preparing the canal for a post.

Evaluation the Relationship between Mandibular Molar Root Apices and Mandibular Canal among Residents of the Moscow Population using Cone-Beam Computed Tomography Technique.

Background: The relationship between the inferior alveolar nerve and the root apices of the mandibular molars and premolars is of clinical importance. The aim of this study was to determine the relationship between the mandibular canal (MC) and the mandibular molar root apices using cone-beam computed tomography (CBCT) scanning among residents of the Moscow population. Materials and Methods: Three hundred CBCT scans for patients aged 20-70 years were analyzed. Patients were divided into three age groups: young group (20-44 years), middle-age group (45-59 years), and elderly group (60-70). The distance from the MC and the mandibular molar root apices was measured in each group in the coronal view of CBCT scans. Statistical analysis was set on P < 0.05. Results: The mean distance from the mesial root apices of the first, second, and third molars to the MC was 4.92, 2.85, and 2.24 mm, respectively. The distal root showed to be the closest root to the MC in multirooted teeth. The young age group showed smaller distances to MC than other age groups (P < 0.05). Females showed smaller distances to MC in mandibular molars than males (P < 0.05). Conclusions: The distance between the root apices and the MC has to be taken into consideration when performing surgical or endodontic procedures.

The Application of Nano Silver Argitos as a Final Root Canal Irrigation for the Treatment of Pulpitis and Apical Periodontitis. In Vitro Study.

BACKGROUND: Endodontic treatment of various forms of pulpitis with variations of root canal system anatomy should be performed with high quality. The use of various antibacterial agents is aimed at maintaining the success of endodontic treatment. The aim of this study was to evaluate the penetration and fixation of the nano-silver solution on the dentinal surface during endodontic treatment. MATERIALS AND METHODS: the study was carried out on 70 extracted single-rooted teeth, randomly divided into two groups. In the teeth of the first group, the smear layer was removed after canal preparation with 17% EDTA solution; in the second group, the smear layer was not removed. In both groups, for the final treatment of the canal, a colloidal 1% solution of нанo серебра nanosilver was used. Samples were cut and prepared for analysis using micro-CT, scanning electron microscopy (SEM), X-ray microanalysis and energy dispersive spectrometry (elemental mapping). RESULTS: in 100% of cases in groups of teeth with a preserved smear layer, the ability of a 1% colloidal solution of nanosilver with particles of 1-2 nm to be fixed on dentin with a removed and preserved smear layer and to leave a film on the dentinal surface was established. In the samples with removed smear layer, silver was found in 73.5% of cases. CONCLUSION: The nano-silver solution with a particle size of 1-2 nm proved its ability to penetrate the dentinal surfaces and create a final film covering the dentinal surface of the root canal before applying the sealer.

Evaluation of the Microbiological Effect of Colloidal Nanosilver Solution for Root Canal Treatment.

BACKGROUND: The goal of endodontic treatment, along with the preparation of the root canal and giving it a shape corresponding to the obturation technique, is the drug treatment of the canal. The aim of this study was to determine the antibacterial effect of a colloidal solution of nanosilver at its various dilutions on root canal microorganism. MATERIALS AND METHODS: A solution of silver nanoparticles at a concentration of 10,000 ppm (1.0%) was diluted in various concentrations (10 solutions from 1% to 0.0025%). Cultures used for research: Str. agalacticae ATCC 3984, E. faecalis ATCC 323, St. aureus ATCC 4785, C. albicans ATCC 10231. After thawing, cultures of microorganisms were introduced into a liquid nutrient medium: cerebral heart broth for bacterial cultures and Sabouraud broth for C. albicans. The cultivation was carried out at a temperature of 37 °C for 24 h. A bacterial suspension for inoculation was prepared from a microbial sediment according to a turbidity standard of 0.5 McFarland in saline. Then, 100 µL of the obtained suspension of microorganisms was inoculated by the "lawn" method using a spatula on the Muller-Hinton medium. Solutions of silver nanoparticles were introduced into wells prepared in agar with a sterile metal punch. Further incubation was carried out for 24 h at 37 °C. RESULTS: colloidal solution of silver nanoparticles at concentrations of 1%, 0.75%, 0.5% inhibited the growth of Str. agalacticae ATCC 3984 with a growth retardation zone of 6-7 mm. The E. faecalis ATCC 29212 strain was sensitive to solutions of silver nanoparticles at concentrations of 1%, 0.75%, 0.5% with a growth inhibition zone of 6-7 mm. Strain St. aureus 4785 demonstrated sensitivity to solutions of silver nanoparticles at concentrations of 1%, 0.75%, 0.5%, 0.1%, 0.05% with a growth retardation zone of 6-8 mm. CONCLUSION: colloidal solutions of silver nanoparticles have antimicrobial action against gram-positive bacteria (Str.agalacticae ATCC 3984, St. aureus ATCC 4785, E. faecalis ATCC 29212) and yeast-like fungi of the genus Candida (C. albicans ATCC 10231, C. albicans 672 and C. albicans D-225M), but this action is strain-specific and depends on the concentration of the solution.

An In Vitro Evaluation Study of the Geometric Changes of Root Canal Preparation and the Quality of Endodontic Treatment.

INTRODUCTION: The geometry of root canals differs in different parts, especially in the apical region, and it is affected by different preparation techniques. The aim of this study was to evaluate the geometric changes of root canal preparation by general dentists regardless of the endodontic instrumentation systems and to study the quality of endodontic treatment by evaluating the untouched areas after mechanical preparation and the smear layer removal. MATERIALS AND METHODS: 100 extracted maxillary canines were collected for the in vitro study from 10 dentists, and the dentists were asked to treat the teeth endodontically. The teeth then were separated and examined under an optical microscope to evaluate the root canal final diameter and the untouched areas. Then, the teeth were examined under a scanned electronic microscope to evaluate the smear layer in coronal, middle, and third parts of the canal. Statistical significance was set as P < 0.05. RESULTS: The mean diameter of the root canal after instrumentation in the coronal and middle thirds was 2.50 ± 1.12 and 1.75 ± 1.24 mm, respectively, and the untouched area percentage observed in the apical thirds was 71%. For smear layer removal, it was better in the coronal and middle thirds than in the apical (P < 0.05). CONCLUSION: The changes in the diameter of the root canal, the percentage of untouched areas after mechanical preparation, and the percentage of smear layer were observed in a higher percent in the apical third than in the coronal and middle thirds, and this raises the question of changing the technique of processing the root canal, especially in the apical third.

Evaluation of the relationship between the maxillary sinus floor and the root apices of the maxillary posterior teeth using cone-beam computed tomographic scanning.

BACKGROUND: The relationship between the maxillary sinus (MS) and the root apices of the posterior teeth is of clinical relevance in diagnosing and treatment planning in the posterior area of the maxilla. This study aimed to assess the relationship between the MS floor and the root apices of the posterior teeth using cone-beam computed tomography (CBCT) scanning. MATERIALS AND METHODS: 325 CBCT scans of patients aged 20-70 years were analyzed. Patients were divided into three age groups: young group (20-44 years), middle age group (45-59 years), and elderly group (60-70). The distance from the MS floor and the root apices of posterior teeth was measured in each group. The relationship between the MS and the posterior roots was also recorded according to Kwak classification. The results were analyzed by IBM statistic SPSS. RESULTS: Type II was most commonly seen in the first and second molars. For premolars, Type I was often observed. The shortest distance to the floor of MS was recorded for the mesiobuccal root of the second molar and the longest distance for the palatal root of the first and second molars. No statistical differences were found between age groups (P > 0.01). CONCLUSION: The anatomical relationship between the MS and the root apices of the posterior teeth is of clinical importance and should be taken into consideration during surgical or endodontic treatment.

Evaluation of Anatomy and Root Canal Morphology of the Maxillary First Molar Using the Cone-Beam Computed Tomography among Residents of the Moscow Region.

BACKGROUND: The success of endodontic treatment depends on the knowledge of root canal system. The root canals have complex morphology and wide individual variations. The aim of this study was to evaluate the root canal morphology of the maxillary first molars among residents of the Moscow region using cone-beam computed tomography (CBCT) scanning. MATERIALS AND METHODS: Three hundred CBCT images of patients aged 20-70 years old were analyzed to study the root canal system (the number of canals and the configuration according to Vertucci's classification) of the maxillary first molars. The prevalence of a second mesiobuccal (MB2) in the mesiobuccal root (MB) was recorded in each age group. RESULTS: three separated roots of the maxillary first molar were observed in 100% of cases. MB2 canals were found in 59.8% of cases. A second distobuccal canal was observed in 0.5% of cases. The canal morphology in the MB root was 40.2% in Type I, 22.4% in Type II, and 37.3% in Type IV. CONCLUSION: The prevalence of MB2 canals was 59.8%, and the most common canal morphology was Vertucci's Type I. Using CBCT scanning is a useful technique to evaluate and analyze the root canal system.

A Cone-Beam Computed Tomography Scanning of the Root Canal System of Permanent Teeth among the Moscow Population.

BACKGROUND: Successful endodontic treatment requires a significant knowledge of root canal anatomy. The aim of this study was to evaluate the root and root canal number of permanent teeth among the Moscow population using cone-beam computed tomography (CBCT) scanning. MATERIALS AND METHODS: 300 CBCT images of subjects were analyzed to study the anatomy of roots and root canal system of each tooth. The collected data were analyzed using IBM SPSS statistics software 22.0 version. RESULTS: The maxillary incisors and canines had one root with one canal in 100%. Maxillary premolars had one root with one or two canals and two roots with two canals, while mandibular premolars were single-rooted with one or two canals. Maxillary first and second molar had three separated roots, and the prevalence of four canals was more often in first molars. Mandibular molars had two roots with different number of canals. CONCLUSION: The root canal system varies greatly among populations and even in different individuals within the same population; thus, using CBCT scanning is an effective technique in investigating the root canal system.