RESUMO
Both cultivation and molecular techniques were used to investigate the microbial diversity and dynamic of a deep-sea vent chimney. The enrichment cultures performed in a gas-lift bioreactor were inoculated with a black smoker chimney sample collected on TAG site on the mid-Atlantic ridge. To mimic as close as possible environmental conditions, the cultures were performed in oligotrophic medium with nitrogen, hydrogen and carbon dioxide (N(2)/H(2)/CO(2)) gas sweeping. Also, the temperature was first settled at a temperature of 85 degrees C and colloidal sulphur was added. Then, the temperature was lowered to 60 degrees C and sulphur was omitted. Archaeal and bacterial diversity was studied in both culture and natural samples. Through 16S rRNA gene sequences analysis of the enrichment cultures microorganisms affiliated to Archeoglobales, Thermococcales were detected in both conditions while, Deferribacterales and Thermales were detected only at 65 degrees C in the absence of sulphur. Single-stranded conformational polymorphism and quantitative PCR permit to study the microbial community dynamic during the two enrichment cultures. The effect of environmental changes (modification of culture conditions), i.e. temperature, medium composition, electron donors and acceptors availability were shown to affect the microbial community in culture, as this would happen in their environment. The effect of environmental changes, i.e. temperature and medium composition was shown to affect the microbial community in culture, as this could happen in their environment. The modification of culture conditions, such as temperature, organic matter concentration, electron donors and acceptors availability allowed to enrich different population of prokaryotes inhabiting hydrothermal chimneys.
Assuntos
Biodiversidade , Reatores Biológicos , RNA Ribossômico 16S/genética , Dióxido de Carbono/química , Técnicas de Cultura de Células/métodos , Meios de Cultura , Elétrons , Meio Ambiente , Concentração de Íons de Hidrogênio , Reação em Cadeia da Polimerase , Polimorfismo Conformacional de Fita Simples , Análise de Sequência de DNA , Enxofre/química , Temperatura , Microbiologia da ÁguaRESUMO
Garden compost leachate was used to form microbial bioanodes under polarization at -0.4, -0.2 and +0.1 V/SCE. Current densities were 6.3 and 8.9 A m(-2) on average at -0.4 and +0.1 V/SCE respectively, with acetate 10 mM. The catalytic cyclic voltammetry (CV) showed similar electrochemical characteristics for all bioanodes and indicated that the lower currents recorded at -0.4V/SCE were due to the slower interfacial electron transfer rate at this potential, consistently with conventional electrochemical kinetics. RNA- and DNA-based DGGE evidenced that the three dominant bacterial groups Geobacter, Anaerophaga and Pelobacter were identical for all bioanodes and did not depend on the polarization potential. Only non-turnover CVs showed differences in the redox equipment of the biofilms, the highest potential promoting multiple electron transfer pathways. This first description of a potential-independent electroactive microbial community opens up promising prospects for the design of stable bioanodes for microbial fuel cells.
Assuntos
Bactérias/metabolismo , Fontes de Energia Bioelétrica/microbiologia , Jardinagem , Solo , Acetatos/análise , Bactérias/genética , Biofilmes/crescimento & desenvolvimento , Eletroforese em Gel de Gradiente Desnaturante , Eletricidade , Técnicas Eletroquímicas , Eletrodos/microbiologia , Genes Bacterianos/genética , RNA Ribossômico 16S/genéticaRESUMO
Recent studies indicate that ammonia is an important electron donor for the oxidation of fixed nitrogen, both in the marine water column and sediments. This process, known as anammox, has so far only been observed in a large range of temperature habitats. The present study investigated the role of anammox in hydrothermal settings. During three oceanographic expeditions to the Mid-Atlantic Ridge, hydrothermal samples were collected from five vent sites, at depths ranging from 750 to 3650 m from cold to hot habitats. Evidence for the occurrence of anammox in these particular habitats was demonstrated by concurrent surveys, including the amplification of 16S rRNA gene sequences related to known anammox bacteria, ladderanes lipids analysis and measurement of a (14)N(15)N dinitrogen production in isotope-pairing experiments at 60 and 85 degrees C. Together these results indicate that new deep-branching anammox bacteria may be active in these hot habitats.