3D tracking of animals in the field using rotational stereo videography.

We describe a method for tracking the path of animals in the field, based on stereo videography and aiming-angle measurements, combined in a single, rotational device. In open environments, this technique has the potential to extract multiple 3D positions per second, with a spatial uncertainty of <1 m (rms) within 300 m of the observer, and <0.1 m (rms) within 100 m of the observer, in all directions. The tracking device is transportable and operated by a single observer, and does not involve any animal tagging. As a video of the moving animal is recorded, track data can easily be completed with behavioural data. We present a prototype device based on accessible components that achieves about 70% of the theoretical maximal range. We show examples of bird ground and flight tracks, and discuss the strengths and limits of the method, compared with existing fine-scale (e.g. fixed-camera stereo videography) and large-scale tracking methods (e.g. GPS tracking).

Social visual contact, a primary "drive" for social animals?

Social animals are always searching for conspecifics, thereby expressing a genuine "social need". This need is illustrated by the fact that social isolation can induce isolation syndromes that can be attenuated by devices such as mirrors. Social contacts appear to be so vital for social animals that they may be ready to work to obtain social stimulations. We used operant conditioning to investigate the possibility to use visual contact (through pictures of conspecifics) as a primary reinforcer. Isolated European starlings (Sturnus vulgaris) were offered the choice of seeing either social images (i.e. pictures of conspecifics) or non-social images (i.e. pictures of landscapes or pictures of monkeys) by triggering sensors. In contrast with most studies, our subjects were presented still pictures of conspecifics and not videos. Moreover, these pictures were used as primary reinforcers and thus were not paired with food. Our data show that starlings were ready to work and to use the apparatus (i.e. sensors) to see pictures in the absence of any other reward. Moreover, they actively and significantly preferred pictures of conspecifics to pictures of inanimate objects (landscapes) or of heterospecifics (monkeys). This suggests that 2D pictures with a social overtone can be used as primary reinforcers for isolated social birds.

Heterogenous Impact of Water Warming on Exotic and Native Submerged and Emergent Plants in Outdoor Mesocosms.

Some aquatic plants present high biomass production with serious consequences on ecosystem functioning. Such mass development can be favored by environmental factors. Temperature increases are expected to modify individual species responses that could shape future communities. We explored the impact of rising water temperature on the growth, phenology, and metabolism of six macrophytes belonging to two biogeographic origins (exotic, native) and two growth forms (submerged, emergent). From June to October, they were exposed to ambient temperatures and a 3 °C warming in outdoor mesocosms. Percent cover and canopy height were favored by warmer water for the exotic emergent Ludwigia hexapetala. Warming did not modify total final biomass for any of the species but led to a decrease in total soluble sugars for all, possibly indicating changes in carbon allocation. Three emergent species presented lower flavonol and anthocyanin contents under increased temperatures, suggesting lower investment in defense mechanisms and mitigation of the stress generated by autumn temperatures. Finally, the 3 °C warming extended and shortened flowering period for L. hexapetala and Myosotis scorpioides, respectively. The changes generated by increased temperature in outdoor conditions were heterogenous and varied depending on species but not on species biogeographic origin or growth form. Results suggest that climate warming could favor the invasiveness of L. hexapetala and impact the structure and composition of aquatic plants communities.

Transdisciplinary Bioblitz: Rapid biotic and abiotic inventory allows studying environmental changes over 60 years at the Biological Field Station of Paimpont (Brittany, France) and opens new interdisciplinary research opportunities.

BACKGROUND: The Biological Field Station of Paimpont (Station Biologique de Paimpont, SBP), owned by the University of Rennes and located in the Brocéliande Forest of Brittany (France), has been hosting student scientific research and field trips during the last 60 years. The study area of the SBP is a landscape mosaic of 17 ha composed of gorse moors, forests, prairies, ponds and creeks. Land use has evolved over time. Historical surveys by students and researchers focused on insects and birds. With this study, we aimed to increase the range of taxa observations, document changes in species composition and landscape and provide a basis for interdisciplinary research perspectives. We gathered historical data, implemented an all-taxon biodiversity inventory (ATBI) in different habitats of the SBP study area, measured abiotic factors in the air, water and soil and performed a photographical landscape observation during the BioBlitz held in July 2017. NEW INFORMATION: During the 24 h BioBlitz, organised by the SBP and the EcoBio lab from the University of Rennes and the French National Center of Scientific Research (CNRS), different habitats were individually sampled. Seventy-seven experts, accompanied by 120 citizens and 12 young people participating in the European Volunteer Service, observed, identified and databased 660 species covering 5 kingdoms, 8 phyla, 21 classes, 90 orders and 247 families. In total, there were 1819 occurrences including records identified to higher taxon ranks, thereby adding one more kingdom and four more phyla. Historical data collection resulted in 1176 species and 4270 occurrences databased. We also recorded 13 climatic parameters, 10 soil parameters and 18 water parameters during the BioBlitz. Current habitats were mapped and socio-ecological landscape changes were assessed with a diachronic approach using 32 historical photographs and historical maps. The coupling of historical biodiversity data with new biotic and abiotic data and a photographic comparison of landscape changes allows an integrative understanding of how the SBP changed from agriculturally-used land to a managed natural area within the last 60 years. Hence, this BioBlitz represents an important holistic sampling of biodiversity for studies on trophic webs or on trophic interactions or on very diverse, but connected, habitats. The integration of social, biotic and abiotic data opens innovative research opportunities on the evolution of socio-ecosystems and landscapes.

Cross-borehole flowmeter tests for transient heads in heterogeneous aquifers.

Cross-borehole flowmeter tests have been proposed as an efficient method to investigate preferential flowpaths in heterogeneous aquifers, which is a major task in the characterization of fractured aquifers. Cross-borehole flowmeter tests are based on the idea that changing the pumping conditions in a given aquifer will modify the hydraulic head distribution in large-scale flowpaths, producing measurable changes in the vertical flow profiles in observation boreholes. However, inversion of flow measurements to derive flowpath geometry and connectivity and to characterize their hydraulic properties is still a subject of research. In this study, we propose a framework for cross-borehole flowmeter test interpretation that is based on a two-scale conceptual model: discrete fractures at the borehole scale and zones of interconnected fractures at the aquifer scale. We propose that the two problems may be solved independently. The first inverse problem consists of estimating the hydraulic head variations that drive the transient borehole flow observed in the cross-borehole flowmeter experiments. The second inverse problem is related to estimating the geometry and hydraulic properties of large-scale flowpaths in the region between pumping and observation wells that are compatible with the head variations deduced from the first problem. To solve the borehole-scale problem, we treat the transient flow data as a series of quasi-steady flow conditions and solve for the hydraulic head changes in individual fractures required to produce these data. The consistency of the method is verified using field experiments performed in a fractured-rock aquifer.

Time-scales of hydrological forcing on the geochemistry and bacterial community structure of temperate peat soils.

Peatlands are an important global carbon reservoir. The continued accumulation of carbon in peatlands depends on the persistence of anoxic conditions, in part induced by water saturation, which prevents oxidation of organic matter, and slows down decomposition. Here we investigate how and over what time scales the hydrological regime impacts the geochemistry and the bacterial community structure of temperate peat soils. Peat cores from two sites having contrasting groundwater budgets were subjected to four controlled drought-rewetting cycles. Pore water geochemistry and metagenomic profiling of bacterial communities showed that frequent water table drawdown induced lower concentrations of dissolved carbon, higher concentrations of sulfate and iron and reduced bacterial richness and diversity in the peat soil and water. Short-term drought cycles (3-9 day frequency) resulted in different communities from continuously saturated environments. Furthermore, the site that has more frequently experienced water table drawdown during the last two decades presented the most striking shifts in bacterial community structure, altering biogeochemical functioning of peat soils. Our results suggest that the increase in frequency and duration of drought conditions under changing climatic conditions or water resource use can induce profound changes in bacterial communities, with potentially severe consequences for carbon storage in temperate peatlands.