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Nanocomposite hydrogels were elaborated that consisted of a physical network formed by an amphiphilic polymer in which C60 fullerene nanoplatelets were embedded. Characterization showed that the nanoplatelets within the polymer network were aggregated. The presence of these nanoplatelets led to an increase of the shear modulus of the hydrogels, that cannot be explained by a filler effect alone. The nanocomposite gels displayed similar rheological behavior, both in linear and non-linear domains, as neat hydrogels at higher polymer concentrations. We suggest that the particles reinforced the gels by forming additional connections between the polymer chains.
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The degradation of (micro)gels and fractal aggregates based on self-assembled amphiphilic triblock copolymers has been investigated in water by confocal microscopy and light scattering respectively. The triblock copolymer consisted of a central hydrophilic poly(acrylic acid) (pAA) block and two hydrophobic end blocks that contained an equal amount of randomly distributed n-butyl acrylate (nBA) and AA units. These latter units helped at tempering the hydrophobic end blocks resulting in the control and the fine tuning of the dynamics of the self-assembled triblock through the pH. Starting from a pH where the dynamics is frozen, the rate of breakup of the macroscopic gels, microgels and of fractal aggregates was measured after increasing the pH to different values. The mechanism of the breakup was found to be independent of the pH, but its rate increased exponentially with increasing pH. The degradation proceeded through the release of the polymers from the bulk into the surrounding aqueous phase.
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In this work, we propose a new protocol for producing model microplastics from an industrial polymer and compare it to a conventional method, cryomilling. Polypropylene industrial pellets were chosen due to their widespread production and frequent presence in the environment, making them a notable source of microplastics. Both protocols start with aging under Ultra-Violet light of the pellets but differ in the subsequent mechanical stress applied-strong vs. soft-to break down the photodegraded pellets into microplastics. All generated particles were fully characterized in terms of size, shape, oxidation rate, and stability in aqueous media. Microplastics produced via cryomilling exhibited significant size and oxidation heterogeneity and tended to aggregate in water. Although the new protocol involving soft mechanical stress required a longer preparation time, it simulated more accurately the environmental degradation of raw plastic. This method successfully produced oxidized microplastics with a controlled size distribution centered around 50⯵m which remained stable in water without stabilizers.
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Protein aggregates were generated by thermal denaturation of whey protein isolates. Depending on the heating conditions, fractal aggregates of various sizes or microgels were obtained. The osmotic compressibility and correlation length of mixtures of fractal aggregates of different sizes were found to be close to the weighted averages of the individual components at the same concentration. The viscosity of these mixtures can be described by a logarithmic mixing law using the weight fraction and the viscosity of the individual components. The same mixing law describes the behavior of mixtures of fractal aggregates and microgels. The effect of the type of protein was investigated by mixing fractal aggregates formed by whey and soy protein isolates. It is suggested that the viscosity of the mixtures is determined by the cooperative movement over length scales much larger than the size of the aggregates.
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Agregados Proteicos , Proteínas de Soja/química , Proteínas do Soro do Leite/química , ViscosidadeRESUMO
Self-assembly of native glycinin at room temperature was investigated as a function of the pH and the NaCl concentration. Microphase separation leading to the formation of dense protein microdomains was observed by confocal laser scanning microscopy. Depending on the conditions, the microdomains coalesced into a continuous protein rich phase or associated into large clusters. Addition of ß-conglycinin inhibited phase separation and reduced the pH range in which it occurred. Microdomains of glycinin that were formed in the presence of 0.1 M NaCl transformed into hollow stable cross-linked microcapsules when heated above 60 °C with diameters between 3 and 30 µm depending on the protein concentration and a shell thickness between 1.0 and 1.4 µm. The microcapsules were stable to dilution in salt free water, whereas microdomains formed at room temperature redispersed. Microdomains formed in mixtures with ß-conglycinin did not transform into microcapsules, but they became stable cross-linked homogeneous microgels.
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Cápsulas , Globulinas/química , Cloreto de Sódio/química , Proteínas de Soja/química , Cápsulas/síntese química , Cápsulas/química , Tamanho da PartículaRESUMO
The phase diagram of mixtures of xyloglucan (XG) and amylopectin (AMP) in aqueous solution is presented. Water-in-water emulsions prepared from mixtures in the two-phase regime were studied in detail, and the interfacial tension was determined. It is shown that the emulsions can be stabilized by addition of ß-lactoglobulin microgels (ßLGm), but only at pH ≤ 5.0. Excess ßLGm preferentially entered the AMP phase at pH > 5.0 and the XG phase at lower pH. The inversion was caused by adsorption of XG onto ßLGm that started below pH 5.5. It is shown that modification of the surface of particles by coating with polysaccharides is a potential lever to control stabilization of water-in-water emulsions.
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Amilopectina/química , Glucanos/química , Lactoglobulinas/química , Xilanos/química , Emulsões , Fluoresceína-5-Isotiocianato , Géis , Concentração de Íons de Hidrogênio , Rodaminas , Tensão Superficial , Água/químicaRESUMO
Heat-induced aggregation and gelation of aqueous solutions of whey protein isolate (WPI) in the presence of sodium caseinate (SC) and CaCl2 was studied at pH 6.6. The effect of adding SC (0-100 g/L) on the structure of the aggregates and the gels was investigated by light scattering and confocal laser scanning microscopy at different CaCl2 concentration ([CaCl2] = 0-30 mM). The gelation process was studied by oscillatory shear rheology. At the whey protein concentrations studied here (34 and 60 g/L), no gels were formed in the absence of CaCl2 and SC. However, WPI solutions gelled above a critical CaCl2 concentration that increased with increasing SC concentration. In the absence of CaCl2, WPI gels were formed only above a critical SC concentration. The critical SC concentration needed to induce WPI gelation decreased weakly when CaCl2 was added. In an intermediate range of CaCl2 concentrations, gels were formed both at low and high SC concentrations, but not at intermediate SC concentrations. Finally, at high CaCl2 concentrations gels were formed at all SC concentrations. The gelation rate and the gel structure of the gels formed at low and high casein concentrations were very different. The effect of SC on the thermal gelation of WPI was interpreted by competition for Ca2+, a chaperon effect, and microphase separation.
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Cálcio/química , Reologia , Proteínas do Soro do Leite/química , Caseínas/química , Caseínas/farmacologia , Géis/química , Temperatura Alta , Concentração de Íons de Hidrogênio , Soluções/química , Proteínas do Soro do Leite/antagonistas & inibidoresRESUMO
In this article, we highlight some recent developments in "smart" physical hydrogels achieved by self-assembling of block type macromolecules. More precisely we focus on two interesting types of gelators namely conventional ionic (or ionogenic) block copolymers and peptide-based polymers having as a common feature their responsiveness to pH and/or temperature which are the main triggers used for potential biomedical applications. Taking advantage of the immense skills of conventional block copolymer hydrogelators, namely macromolecular design, self-assembling mechanism, gel rheological properties, responsiveness to various triggers and innovative applications, the development of novel self-assembling gelators, integrating the new knowledge emerging from the peptide-based systems, opens new horizons towards bio-inspired technologies.
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Hidrogéis/química , Peptídeos/química , Polímeros/química , Eletrólitos/química , Concentração de Íons de Hidrogênio , Interações Hidrofóbicas e Hidrofílicas , Estrutura Secundária de Proteína , TemperaturaRESUMO
Solutions of the globular whey protein ß-lactoglobulin (ß-lg) were heated at different protein concentrations leading to the formation of polydisperse fractal aggregates with different average sizes. The structure of the solutions was analyzed with light scattering as a function of the protein concentration. The osmotic compressibility and the dynamic correlation length decreased with increasing concentration and became independent of the aggregate size in dense suspensions. The results obtained for different aggregate sizes could be superimposed after normalizing the concentration with the overlap concentration. Dense suspensions of fractal protein aggregates are strongly interpenetrated and can be visualized as an ensemble of fractal 'blobs'. The viscosity of the heated ß-lg solutions increased extremely sharply above 80 g L(-1) and diverged at 98 g L(-1), mainly due to the sharply increasing aggregate size. At a fixed aggregate size, the viscosity increased initially exponentially with increasing concentration and then diverged. The increase was stronger when the aggregates were larger, but the dependence of the viscosity on the aggregate size was weaker than that of the osmotic compressibility and the dynamic correlation length. The concentration dependence of the viscosity of solutions of fractal ß-lg aggregates is much stronger than that of homogeneous ß-lg microgels. The behavior of fractal aggregates formed by whey protein isolates was similar.
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Géis/química , Lactoglobulinas/química , Agregados Proteicos , Animais , Temperatura Alta , Reologia , Viscosidade , Proteínas do Soro do Leite/químicaRESUMO
Interpenetrated gels of biocompatible polysaccharides alginate and silanized hydroxypropyl methyl cellulose (Si-HPMC) have been studied in order to assess their potential as scaffolds for the regeneration of human tissues. Si-HPMC networks were formed by reduction of the pH to neutral and alginate networks were formed by progressive in situ release of Ca(2+). Linear and non-linear mechanical properties of the mixed gels at different polymer and calcium concentrations were compared with those of the corresponding single gels. The alginate/Si-HPMC gels were found to be stiffer than pure Si-HPMC gels, but weaker and more deformable than pure alginate gels. No significant difference was found for the maximum stress at rupture measured during compression for all these gels. The degrees of swelling or contraction in excess water at pH 7 as well as the release of Ca(2+) was measured as a function of time. Pure alginate gels contracted by as much as 50 % and showed syneresis, which was much reduced or even eliminated for mixed gels. The important release of Ca(2+) upon ageing for pure alginate gels was much reduced for the mixed gels. Furthermore, results of cytocompatibility assays indicated that there was no cytotoxicity of Si-HPMC/alginate hydrogels in 2D and 3D culture of human SW1353 cells. The results show that using interpenetrated Si-HPMC/alginate gels has clear advantages over the use of single gels for application in tissue regeneration.
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Alginatos/química , Regeneração Tecidual Guiada/métodos , Hidrogéis/química , Alicerces Teciduais/química , Materiais Biocompatíveis , Linhagem Celular , Sobrevivência Celular , Ácido Glucurônico/química , Ácidos Hexurônicos/química , Humanos , Derivados da Hipromelose/química , Teste de Materiais , Silanos/química , Fatores de Tempo , ÁguaRESUMO
There has been much interest in the construction of soft nanomaterials in solution due to a desire to emulate the exquisite structure and function of Nature's equivalents (e.g. enzymes, viruses, proteins and DNA). Nature's soft nanomaterials are capable of selectivity, precision and efficiency in areas such as information storage and replication, transportation and delivery, and synthesis and catalysis. To this end, the use of small molecules, amphiphiles, colloids, and polymers have been investigated for the development of advanced materials in myriad fields of biomedicine and synthetic chemistry. Two major challenges are faced in this area of research: the reproducible, scalable and precise synthesis of such constructs and the reliable, accurate and in-depth analysis of these materials. This tutorial review will focus on this second aspect and provide a guide for the characterisation and analysis of soft nanomaterials in solution using scattering and microscopic techniques.
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Nanoestruturas/análise , Polímeros/química , Luz , Microscopia de Força Atômica , Nanoestruturas/química , Difração de Nêutrons , Espalhamento de Radiação , Espalhamento a Baixo Ângulo , Difração de Raios XRESUMO
Dynamic mechanical properties of aqueous solutions of hydroxypropyl methyl cellulose (HPMC) were investigated using oscillatory shear measurements. The structure was investigated with light scattering. A systematic investigation of the frequency dependence of the shear moduli showed that HPMC forms two distinct interpenetrating networks. A transient network is formed above about 0.3 wt % by reversible cross-linking of the chains. The elastic modulus of this network is independent of the temperature, but increases linearly with the concentration. An independent permanent network is formed involving a small fraction of the polymers and has an elastic modulus that increases with increasing temperature. Its elastic modulus is history dependent and evolves slowly with time. The transient network collapses at a critical temperature where micro phase separation occurs, but the permanent network is not influenced by this phenomenon. Light scattering showed that the pore size of the transient network is less than 40 nm, while probe diffusion measurements showed that the pore size of the permanent network is larger than 1 µm.
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Metilcelulose/análogos & derivados , Derivados da Hipromelose , Metilcelulose/análise , Metilcelulose/química , Resistência ao CisalhamentoRESUMO
Various polymeric micelles were formed from amphiphilic block copolymers, namely, poly(ethyleneoxide-b-ε-caprolactone), poly(ethyleneoxide-b-d,l-lactide), and poly(ethyleneoxide-b-styrene). The micelles were characterized by static and dynamic light scattering, electron microscopy, and asymmetrical flow field-flow fractionation. They all displayed a similar size close to 20 nm. The influence of the chemical structure of the block copolymers on the stability upon dilution of the polymeric micelles was investigated to assess their relevance as carriers for nanomedicine. In the same manner, the stability upon aging was assessed by FRET experiments under various experimental conditions (alone or in the presence of blood proteins). In all cases, a good stability over 48 h for all systems was encountered, with PDLLA copolymer-based systems being the first to release their load slowly. The cytotoxicity and photocytotoxicity of the carriers were examined with or without their load. Lastly, the photodynamic activity was assessed in the presence of pheophorbide a as photosensitizer on 2D and 3D tumor cell culture models, which revealed activity differences between the 2D and 3D systems.
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Portadores de Fármacos/química , Fotoquimioterapia/métodos , Fármacos Fotossensibilizantes/química , Polímeros/química , Técnicas de Cultura de Células/métodos , Clorofila/análogos & derivados , Clorofila/química , Clorofila/farmacologia , Portadores de Fármacos/toxicidade , Estabilidade de Medicamentos , Transferência Ressonante de Energia de Fluorescência , Células HCT116/efeitos dos fármacos , Humanos , Lactonas/química , Luz , Micelas , Fármacos Fotossensibilizantes/farmacologia , Poliésteres/química , Polietilenoglicóis/química , Espalhamento de Radiação , Relação Estrutura-AtividadeRESUMO
Polymersomes formed from amphiphilic block copolymers, such as poly(ethyleneoxide-b-ε-caprolactone) (PEO-b-PCL) or poly(ethyleneoxide-b-methylmethacrylate), were characterized by asymmetrical flow field-flow fractionation coupled with quasi-elastic light scattering (QELS), multi-angle light scattering (MALS), and refractive index detection, leading to the determination of their size, shape, and molecular weight. The method was cross-examined with more classical ones, like batch dynamic and static light scattering, electron microscopy, and atomic force microscopy. The results show good complementarities between all the techniques; asymmetrical flow field-flow fractionation being the most pertinent one when the sample exhibits several different types of population.
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Fracionamento por Campo e Fluxo/instrumentação , Luz , Metilmetacrilato/química , Poliésteres/química , Espalhamento de Radiação , Tensoativos/química , Desenho de Equipamento , Tamanho da PartículaRESUMO
The development of fully biobased hydrogels obtained by simple routes and in the absence of toxic or environmentally harmful reagents is a major challenge in meeting new societal demands. In this work, we discuss the development of hydrogels made from cellulose nanocrystals (CNCs) and xyloglucan (XG), two non-toxic, renewable, and biobased components. We present three strategies to fine-tune the functional properties. The first one consists in varying the XG/CNC ratio that leads to the modulation of the mechanical properties of hydrogels as well as a better comprehension of the gel mechanism formation. The second relies on tuning the XG chains' interaction by enzymatic modification to achieve thermoresponsive systems. Finally, the third one is based on the increase in the hydrogel solid content by osmotic concentration. The high-solid-content gels were found to have very high mechanical properties and self-healing properties that can be used for molding materials. Overall, these approaches are a case study of potential modifications and properties offered by biobased nanocolloidal hydrogels.
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We have studied the self-assembly of aqueous dispersions of amphiphilic di- and triblock copolyelectrolytes using static and dynamic light scattering. The hydrophobic blocks contained both ionisable and hydrophobic units rendering the association dynamic and thus ensuring that thermodynamic equilibrium was reached. The incorporation of ionisable units into the hydrophobic blocks caused the self-assembly to be strongly influenced by the pH and the ionic strength. As in the case of neutral block copolymers, diblock copolyelectrolytes self-assembled into star-like micelles and triblock copolyelectrolytes formed flower-like micelles. The latter was not predicted to occur for block copolyelectrolytes. At higher concentrations a system spanning network was formed. The structure of the systems could be quantitatively described by a model of purely repulsive spheres for the diblocks and attractive spheres for the triblocks. The polyelectrolyte effect expressed itself by a sensitivity of the structure to the pH and the ionic strength. The attraction increased with decreasing pH and increasing ionic strength leading at high ionic strength to phase separation.
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Eletrólitos/química , Micelas , Concentração de Íons de Hidrogênio , Interações Hidrofóbicas e Hidrofílicas , Íons/química , Luz , Concentração Osmolar , Espalhamento de Radiação , Sódio/químicaRESUMO
The self-assembly in water of an amphiphilic P(nBMA(50%) -stat-DMAEMA(50%) )(100)-b-PDMAEMA(235) diblock copolymer based on hydrophilic dimethylaminoethylmethacrylate (DMAEMA) units and hydrophobic n-butylmethacrylate (nBMA) ones is reported. DMAEMA units have been incorporated into the hydrophobic block of this copolymer to moderate its hydrophobic character. Light scattering experiments revealed the formation of micelles whose apparent aggregation number varied reversibly with the ionization degree of the DMAEMA units. Incorporating hydrophilic units into the hydrophobic block of an amphiphilic block copolymer is thus a way to generate dynamic aggregates in aqueous medium. As this strategy was also successful using other types of hydrophilic units, we believe it to be universal.
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Metacrilatos/química , Ácidos Polimetacrílicos/química , Tensoativos/química , Anisóis/química , Concentração de Íons de Hidrogênio , Interações Hidrofóbicas e Hidrofílicas , Luz , Metacrilatos/síntese química , Micelas , Tamanho da Partícula , Polimerização , Ácidos Polimetacrílicos/síntese química , Espalhamento de Radiação , Cloreto de Sódio/química , Solventes/química , Tensoativos/síntese químicaRESUMO
HYPOTHESIS: To disperse high concentration of C60 fullerene in water, we propose to use an emulsification-evaporation process in the presence of an amphiphilic polymer whose chemical structure has been chosen for inducing specific interaction with fullerene The viscosity enhancement provided by self-assembly of the amphiphilic polymers in water should result in high stability of the suspensions. The organic solvent has also to been chosen so as to maximize the initial fullerene concentration. EXPERIMENTS: The concentrations of polymer and fullerene, the solvent type and the volume fraction of the organic phase have been varied. Their influence on the concentration of the fullerene dispersions and on the size and shape of the resulting nanoparticles have been investigated by UV-Visible spectroscopy, light scattering and cryo-transmission electron microscopy experiments. FINDINGS: The resulting nanoparticles consist of aggregates of C60 fullerene stabilized by the cationic polymer with morphologies/sizes tunable through fullerene and polymer concentration. At high fullerene concentration, nanoplatelets are obtained that consist in thin 2D nanocrystals. Their suspensions are very stable with time due to the viscosity of the dispersing aqueous medium. The concentration of fullerene nanoparticles dispersed in water is as high as 8 g/L which corresponds to an upper limit that has never been reached so far.
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A benzo[ghi]perylenetriimide (BPTI) derivative bearing a terminal azido group on the expanded π-conjugated backbone has been synthesized and characterized. This promising photo- and electroactive BPTI motif has been used to obtain an original penta(organo)fullerene as a promising multi-electron acceptor system. Our studies show its self-assembly resulting from aggregation via π-π stacking interaction in solution and in the solid state.
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This study deals with the fine-tuning of the interactions between silica nanoparticles and a LCST polymer in order to build permanent rigid linear aggregates. LCST polymers become hydrophobic and collapse above a critical temperature. The collapse of the polymer chains at the surface of the silica particles generates an attractive potential that can overcome the repulsive electrostatic forces between the silica particles under certain circumstances. The combined use of the thermoresponsiveness of poly(ethylene oxide) and of the chemical condensation properties of silica enables us to build permanent rigid aggregates displaying rodlike shapes just by increasing the temperature. These aggregates have been characterized using two complementary techniques: transmission electron microscopy and small angle neutron scattering. For low curing time, it appears that small linear aggregates are obtained when the electrostatic surface potential (pH = 8.5) is high and the initial ionic strength is low (I approximately = 10(-3) M). For higher heating time these objects aggregate further leading to some branching and ultimately to 3D gels which phase separate.