RESUMO
We have prepared two fluorescent DNA probes--UDBF and UDBT, containing 2-ethynyldibenzofuran and 2-ethynyldibenzothiophene moieties, respectively, covalently attached to the base dU--and incorporated them in the central positions of oligodeoxynucleotides (ODNs) so as to develop new types of quencher-free linear beacon probes and investigate the effect of functionalization of the fluorene scaffold on the photophysical properties of the fluorescent ODNs. The ODNs containing adenine flanking bases (FBs) displayed a selective fluorescence "turn-off" response to mismatched targets with guanine bases; this suggests that these probes could be used as base-discriminating fluorescent nucleotides. On the other hand, we observed a "turn-on" response to matched targets when the UDBF and UDBT units of ODNs containing pyrimidine-based FBs were positioned opposite the four natural nucleobases. In particular, an ODN incorporating UDBT and cytosine FBs has potential use in single-nucleotide polymorphism typing.
Assuntos
Benzofuranos/química , Sondas de DNA/metabolismo , Desoxiuridina/análogos & derivados , Desoxiuridina/química , Corantes Fluorescentes/química , Tiofenos/química , Pareamento Incorreto de Bases , Dicroísmo Circular , Sondas de DNA/síntese química , Sondas de DNA/química , Transporte de Elétrons , Oligodesoxirribonucleotídeos/química , Oligodesoxirribonucleotídeos/metabolismo , Polimorfismo de Nucleotídeo Único , Espectrometria de Fluorescência , Temperatura de TransiçãoRESUMO
We examined microenvironment-sensitive fluorescent 2'-deoxyuridines labeled with fluorene derivatives that exhibited solvent-dependent photophysical properties. The high sensitivity of the fluorescence shift and the nucleoside intensity dependence on solvent polarity provided information useful for estimating the polarity of the environment surrounding the fluorescent nucleoside.
Assuntos
Desoxiuridina/síntese química , Fluorenos/química , Corantes Fluorescentes/química , Desoxiuridina/química , Solventes , Espectrometria de Fluorescência , Coloração e RotulagemRESUMO
Sclerosing stromal tumor (SST) was first delineated as a distinct ovarian sex cord stromal tumor in 1973 by Chalvardjian and Scully. It is a benign neoplasm, distinguished from other ovarian stromal tumors by the production of collagen and a pseudolobular pattern, and it tends to occur in the second and third decades of life in diagnosed patients. We discovered two rare cases of SST in post-menopausal women which are the topic of this report. These case studies are accompanied by a brief review of the literature.
RESUMO
[This corrects the article on p. 115 in vol. 21, PMID: 26356607.].