A Higher Kick Frequency Swimming Training Program Optimizes Swim-to-Cycle Transition in Triathlon.

ABSTRACT: Ambrosini, L, Presta, V, Vitale, M, Menegatti, E, Guarnieri, A, Bianchi, V, De Munari, I, Condello, G, and Gobbi, G. A higher kick frequency swimming training program optimizes swim-to-cycle transition in triathlon. J Strength Cond Res 38(5): 976-984, 2024-The purpose of this study was to evaluate the effect of an 8-week swimming training program on biomechanical and physiological responses during a swim-to-cycle simulation. Fifteen triathletes were randomly allocated to 3 groups: a 6-beat-kick group (K6), a 4-beat-kick group (K4), and a control group (CG). Biomechanical and physiological parameters were evaluated during a 400-m swim and a 10-minute cycle segment before (Pretraining) and after (Posttraining) the program. A lower stroke frequency ( p = 0.004) and a higher stroke length ( p = 0.002) was found in K6 compared with CG at Posttraining. A reduction in the K6 emerged between Pretraining and Posttraining during cycling for heart rate ( p = 0.005), V̇O 2 ( p = 0.014), and energy expenditure ( p = 0.008). A positive association emerged between swim kick index and cycling cadence in the K6 group. The improvement in stroke frequency and length observed in the K6 group could be explained as an improvement in swimming technique. Similarly, the reduction in energy expenditure during cycling at Posttraining for the K6 group suggests an improvement in the working economy. Triathlon coaches and athletes should consider the inclusion of high swim kick into their training programs to enhance swim and cycling performance, which can ultimately lead to an improvement in the swim-to-cycle transition and the overall triathlon performance.

Exploring correlates of physical activity behaviour in UK children and their inter-relationships using a multidisciplinary approach: A concept mapping study.

It is still unknown which correlates of physical activity behaviour (PAB) may be effective and how they may influence PAB in UK children. The objective of the current study was to generate a conceptual analysis of the correlates of PAB in UK children (5-12 years) using the input of researchers in the field of physical activity (PA experts; PAE) and other fields (non-PA experts; non-PAE). A concept mapping approach was used to identify potential (new) correlates of PAB in children, assess their importance based on rating of potential modifiability and effect, and generate a concept map depicting the associations between them. In the first (brainstorming) stage (n = 32 experts) yielded 93 correlates, including 14 new correlates not identified in previous reviews. In the second (rating and sorting) stage (n = 26 experts), 32 correlates were rated as important and a four-cluster concept map was generated including themes related to Society/community, Home/social setting, Personal/social setting and Psychological/emotional correlates. Two additional concept maps were generated for PAE and non-PAE. From expert opinion, we identified new correlates of PAB that warrant further research and we highlight the need to consider the interaction between intrapersonal and external correlates when designing interventions to promote PA in UK children.

The influence of maturity on recovery and perceived exertion, and its relationship with illnesses and non-contact injuries in young soccer players.

A high training load could increase the risk of injury in soccer players. However, there is still a lack of knowledge regarding the effect on young athletes, in whom a different maturity status could lead to different physiological responses to training. Therefore, the aim of this study was to investigate the association of internal load markers and recovery status with risk of illnesses and non-contact injuries in young soccer players characterised by different maturity status. Twenty-three U14 soccer players were monitored during a full season and categorised according to years from peak height velocity (PHV). Based on the estimated values, athletes were divided into three different groups: Pre-PHV, Circa-PHV and Post-PHV players. The following internal load markers were monitored: rating of perceived exertion (RPE), session rating of perceived exertion (S-RPE), weekly load (WL), cumulative loads for 2, 3, and 4 weeks (WL2, WL3, WL4), acute to chronic workload ratio for 2, 3 and 4 weeks (A:C2, A:C3, A:C4) and week-to-week percentage variation (%WL). Recovery status was quantified using the total quality recovery (TQR) scale. Z-score transformation was adopted for TQR and RPE values and the difference between the parameters was calculated (Z-TQR-RPE). The Kruskal-Wallis test was adopted to evaluate differences in TQR and RPE with respect to maturity offset. A multinomial regression analysis was performed to evaluate the association between internal load markers and risk of illness and non-contact injuries. The variables that showed a significant association were included in the receiver operating characteristic (ROC) curve analysis. The results revealed that RPE was significantly higher (p < 0.01) and TQR significantly lower (p < 0.05) in Post-PHV compared to Pre- and Circa-PHV. Moreover, RPE, A:C4, TQR and Z-TQR-RPE showed a significant (p < 0.01) association with non-contact injuries. The internal load markers included in ROC curve analysis showed poor predictive ability (AUC ≤ 0.6). A rapid increase in training load together with a decrease in recovery status may produce higher susceptibility to illnesses and non-contact injuries. The contrasting physiological responses found in relation to maturity status could explain the different injury predisposition in young soccer players.

Effects of a Judo Training on Functional Fitness, Anthropometric, and Psychological Variables in Old Novice Practitioners.

This study aimed to investigate the effects of a 4-month judo training (1 hr session, biweekly) on physical and mental health of older adults (69.7 ± 4.2 years). Participants (N = 30) were assigned to a judo novice practitioners group (n = 16) or a control group (n = 14), which did not receive any training. Before and after the program, they underwent anthropometric (body mass index and waist and hip circumferences); functional fitness (upper and lower body flexibility and strength, coordination); and psychological assessments (perceived physical and mental health, body image, and fear of falling). The judo group showed reductions of waist circumference (Δ = -1%, d = 0.2) and improvements for lower and upper body flexibility (Δ = +69%, d = 0.4 and Δ = +126%, d = 0.5, respectively) and strength (Δ = +12%, d = 0.6 and Δ = +31%, d = 1.6, respectively). The control group showed a decline in lower body strength (Δ = -12%, d = 0.8). Psychological variables did not reveal statistically significant effects. Judo seems beneficial for improving anthropometric and functional fitness variables, relevant aspects of successful aging.

Technical and Tactical Aspects in Italian Youth Rugby Union in Relation to Different Academies, Regional Tournaments, and Outcomes.

Ungureanu, AN, Condello, G, Pistore, S, Conte, D, and Lupo, C. Technical and tactical aspects in Italian youth rugby union in relation to different academies, regional tournaments, and outcomes. J Strength Cond Res 33(6): 1557-1569, 2019-This study aimed to analyze the technical and tactical aspects of the Italian under-18 Academy Rugby Union in relation to different academies, regional tournaments, and game outcomes. A notational analysis (44 indicators) was performed on 16 games (2014-15 season) to evaluate strong differences (p ≤ 0.05; moderate-large effect sizes [ESs]) according to variables. Among academies, strong differences were showed for defensive breakdown, in which the defending support is much (range = 77-87%), equal (range = 11-32%), and less (range = 2-12%) numerous than the attacking support, total tackles (range = 64-122), and passes (range = 72-151), pass to possession ratio (range = 6-10), possession lost due to an error (range = 28-59%), and ball in play in own (range = 8-25%) and opponent (range = 7-31%) 22-m area indicators. For tournaments, effects emerged for offensive breakdown when the ball is used quickly using maximum 2 attacking supports (range = 20-30%) and is not used quickly (range = 28-41%), total penalty kicks (range = 11-16), and sequences period 0-10 (range = 26-35%) and 10-40 seconds (range = 47-55%). Conversely, winning and losing academies reported differences with small ESs. These results highlight that the technical and tactical aspects of the Italian under-18 Academy Rugby Union are quite homogeneous, suggesting that FIR coaching staffs are more oriented to players' skills than successful games. However, tactical and strength and conditioning coaches can benefit from the findings of this study, focusing training on cognitive, strength, and repeated sprint abilities with and without change of direction for improving the occurrence of "set pieces won/regained" and "ball in play in opponent 22 m area," which appear as the key of the game in this rugby competition level.

Effects of Judo Training on Bones: A Systematic Literature Review.

Ciaccioni, S, Condello, G, Guidotti, F, and Capranica, L. Effects of judo training on bones: a systematic literature review. J Strength Cond Res 33(10): 2882-2896, 2019-The aim of this study was to provide a systematic literature review on the osteogenic effects of judo (PROSPERO: CRD42016041803). The online search was conducted according to the inclusion criteria: observational studies and clinical/interventional trials in English from inception to June 2016; bone health, bone mineral density (BMD), diameters, impact force, and bone turnover marker (BTM) outcomes. Thirty-four studies were included and graded for their methodological quality ("fair" quality: 79.4%). The most used assessment method (44.1%) was X-ray absorptiometry. A positive association between judo and bone health/status emerged. Findings support site-specific BMD accrual in children, adolescents, adult athletes, and in premenopausal and postmenopausal female practitioners. Bone turnover markers revealed a hypermetabolic status in high-level judo athletes. The osteogenic stimuli of judo seem to protect athletes from alterations in bone metabolic balance due to weight loss cycling. Sexual dimorphism was found between judoka in bone diameters and mass, and significant differences in bone breadths emerged between elite and nonelite judokas. The fall techniques reduced bone impact force and velocity with respect to "natural" fall. Further longitudinal, cross-sectional, and interventional researches are required. This article provides useful information on bone for health sport scientists, coaches, and practitioners, stimulating future research lines on judo. In particular, coaches and physical trainers should consider introducing judo fall techniques in their training plans to prevent fall-related injuries, especially relevant in the older population. Conversely, coaches are urged to carefully control weight cycling dietary habits of their athletes, which can produce serious metabolic responses on bones.

Policy determinants of physical activity across the life course: a 'DEDIPAC' umbrella systematic literature review.

Background: Despite the large number of studies and reviews available, the evidence regarding the policy determinants of physical activity (PA) is inconclusive. This umbrella systematic literature review (SLR) summarizes the current evidence on the policy determinants of PA across the life course, by pooling the results of the available SLRs and meta-analyses (MAs). Methods: A systematic online search was conducted on MEDLINE, ISI Web of Science, Scopus and SPORTDiscus databases up to April 2016. SLRs and MAs of observational studies investigating the association between policy determinants of PA and having PA as outcome were considered eligible. The extracted data were assessed based on the importance of the determinants, the strength of evidence and the methodological quality. Results: Fourteen reviews on 27 policy determinants of PA were eligible for this umbrella SLR. The majority of the reviews were of moderate quality. Among children, a clear association between time spent outdoors and PA emerged. Among adults, working hours were negatively associated with PA, though evidence was limited. At the population level, community- and street-scale urban design and land use policies were found to positively support PA levels, but levels of evidences were low. Conclusions: With this umbrella SLR the policy determinants of PA at individual-level and population-level have been summarized and assessed. None of the investigated policy determinants had a convincing level of evidence, and very few had a probable level of evidence. Further research is needed, preferably by using prospective study designs, standardized definitions of PA and objective measurement of PA.

Behavioral determinants of physical activity across the life course: a "DEterminants of DIet and Physical ACtivity" (DEDIPAC) umbrella systematic literature review.

BACKGROUND: Low levels of physical activity (PA) are a global concern and increasing PA engagement is becoming a priority in current public health policies. Despite the large number of studies and reviews available, the evidence regarding the behavioral determinants of PA is still inconclusive. Thus, the aim of this umbrella systematic literature review (SLR) was to summarize the evidence on the behavioral determinants of PA across the life course. METHODS: A systematic online search was conducted on MEDLINE, ISI Web of Science, Scopus, and SPORTDiscus databases. The search was limited to studies published in English from January, 2004 to April, 2016. SLRs and meta-analyses (MAs) of observational studies that investigated the behavioral determinants of PA were considered eligible. The extracted data were assessed based on the importance of the determinants, the strength of evidence, and the methodological quality. The full protocol is available from PROSPERO (PROSPERO 2014:CRD42015010616). RESULTS: Seventeen reviews on 35 behavioral determinants of PA were eligible for this umbrella SLR. Regardless of age, the most investigated determinants were those related with 'screen use' and 'smoking'. For youth, probable positive evidence emerged for 'previous PA' and 'independent mobility and active transport' among children and adolescents. For the adult population, 'transition to university' and 'pregnancy/having a child' showed probable negative associations. CONCLUSIONS: Although the majority of the evidence was limited and most of the determinants were not associated with PA, this umbrella SLR provided a comprehensive overview of the associations between behavioral determinants and PA. Youth should be physically active in the early years and increase active transportation to/from school, independent mobility, and 'free-range activities' without adult supervision, whilst adult PA behaviors are mostly influenced by the life events. Finally, more research is needed that incorporates prospective study designs, standardized definitions of PA, objective measurement methods of PA assessment, and the use of interactionist and mediational approaches for the evaluation of different behavioral determinants influencing PA behaviors.

Socio-cultural determinants of physical activity across the life course: a 'Determinants of Diet and Physical Activity' (DEDIPAC) umbrella systematic literature review.

OBJECTIVE: Regular physical activity (PA) reduces the risk of disease and premature death. Knowing factors associated with PA might help reducing the disease and economic burden caused by low activity. Studies suggest that socio-cultural factors may affect PA, but systematic overviews of findings across the life course are scarce. This umbrella systematic literature review (SLR) summarizes and evaluates available evidence on socio-cultural determinants of PA in children, adolescents, and adults. METHODS: This manuscript was drafted following the recommendations of the 'Preferred Reporting Items for Systematic reviews and Meta-Analyses' (PRISMA) checklist. The MEDLINE, Web of Science, Scopus, and SPORTDiscus databases were searched for SLRs and meta-analyses (MAs) on observational studies published in English that assessed PA determinants between January 2004 and April 2016. The methodological quality was assessed and relevant information on socio-cultural determinants and any associations with PA was extracted. The available evidence was evaluated based on the importance of potential determinants and the strength of the evidence. RESULTS: Twenty SLRs and three MAs encompassing 657 eligible primary studies investigated potential socio-cultural PA determinants, with predominantly moderate methodological quality. Twenty-nine potential PA determinants were identified that were primarily assessed in children and adolescents and investigated the micro-environmental home/household level. We found probable evidence that receiving encouragement from significant others and having a companion for PA were associated with higher PA in children and adolescents, and that parental marital status (living with partner) and experiencing parental modeling were not associated with PA in children. Evidence for the other potential determinants was limited, suggestive, or non-conclusive. In adults, quantitative and conclusive data were scarce. CONCLUSIONS: A substantial number of SLRs and MAs investigating potential socio-cultural determinants of PA were identified. Our data suggest that receiving social support from significant others may increase PA levels in children and adolescents, whereas parental marital status is not a determinant in children. Evidence for other potential determinants was limited. This was mainly due to inconsistencies in results on potential socio-cultural determinants of PA across reviews and studies. TRIAL REGISTRATIONS: This umbrella SLR was recorded on PROSPERO (Record ID: CRD42015010616 ).

Cytotoxic and Apoptotic Activities of Prunus spinosa Trigno Ecotype Extract on Human Cancer Cells.

The aim of this work was to demonstrate that a natural compound, not-toxic to normal cells, has cytotoxic and sensitizing effects on carcinoma cells, with the final goal of combining it with chemotherapeutic drugs to reduce the overall dose. Prunus spinosa Trigno ecotype (PsT) drupe extract with a nutraceutical activator complex (NAC) made of amino acids, vitamins and mineral salt blends, has shown in vitro anticancer activity. The cytotoxic effect of (PsT + NAC)® has been evaluated on human cancer cells, with an initial screening with colorectal, uterine cervical, and bronchoalveolar cells, and a subsequent focus on colon carcinoma cells HCT116 and SW480. The viability reduction of HCT116 and SW480 after treatment with (PsT 10 mg/mL + NAC)® was about 40% (p < 0.05), compared to control cells. The cell's survival reduction was ineffective when the drug vehicle (NAC) was replaced with a phosphate buffer saline (PBS) or physiological solution (PS). The flow cytometry evaluation of cancer cells' mitochondrial membrane potential showed an increase of 20% depolarized mitochondria. Cell cycle analysis showed a sub G1 (Gap 1 phase) peak appearance (HCT116: 35.1%; SW480: 11.6%), indicating apoptotic cell death induction that was confirmed by Annexin V assay (HCT116: 86%; SW480: 96%). Normal cells were not altered by (PsT + NAC)® treatments.

Using concept mapping in the development of the EU-PAD framework (EUropean-Physical Activity Determinants across the life course): a DEDIPAC-study.

BACKGROUND: A large proportion of European children, adults and older adults do not engage in sufficient physical activity (PA). Understanding individual and contextual factors associated with PA behaviours is essential for the identification and implementation of effective preventative environments, policies, and programmes that can promote an active lifestyle across life course and can potentially improve health. The current paper intends to provide 1) a multi-disciplinary, Pan-European and life course view of key determinants of PA behaviours and 2) a proposal of how these factors may cluster. METHODS: After gathering a list of 183 potential PA behaviours-associated factors and a consensus meeting to unify/consolidate terminology, a concept mapping software was used to collate European experts' views of 106 identified factors for youth (<19 years), adults (19-64 years), and older adults (≥65 years). The analysis evaluated common trends in the clustering of factors and the ratings of the distinct factors' expected modifiability and population-level impact on PA behaviours across the life course. Priority for research was also assessed for each cluster. RESULTS: The concept mapping resulted in six distinct clusters, broadly merged in two themes: 1) the 'Person', which included clusters 'Intra-Personal Context and Wellbeing' and 'Family and Social Economic Status' (42 % of all factors) and 2) the 'Society', which included the remaining four clusters 'Policy and Provision', 'Cultural Context and Media', 'Social Support and Modelling', and 'Supportive Environment' (58 % of all factors). Overall, 25 factors were rated as the most impactful on PA behaviours across the life course and being the most modifiable. They were mostly situated in the 'Intra-Personal Context and Wellbeing' cluster. Furthermore, 16 of them were rated as top priority for research. CONCLUSIONS: The current framework provides a preliminary overview of factors which may account for PA behaviour across the life course and are most relevant to the European community. These insights could potentially be a foundation for future Pan-European research on how these factors might interact with each other, and assist policy makers to identify appropriate interventions to maximize PA behaviours and thus the health of European citizens.

Women's water polo world championships: technical and tactical aspects of winning and losing teams in close and unbalanced games.

This study aimed to compare the technical and tactical aspects between winning and losing teams in close (i.e., 1-3 goals of difference) and unbalanced (i.e., >3 goals of difference) elite women's water polo games. A notational analysis was performed on 45 games to evaluate occurrence of action, mean duration, action outcome, origin and execution of shot, offensive and defensive role, and arrangement of even, counterattack, power play, and transition situations. Independent 2-sided t-tests were applied to show differences (p < 0.05) between teams. Regarding close games, effects emerged for action outcome (counterattack: lost possessions; power play: goals, no goal shots), origin (even: zone 1, zone 4; counterattack: zone 1, zone 5) and execution (even: off-the-water shots; counterattack: shots after > than 2 fakes; power play: drive shots) of shots, offensive even arrangements (6 vs. 6/5 vs. 5; 2 vs. 2/1 vs. 1), and role. Regarding unbalanced games, differences emerged for the occurrence of action (even, counterattack), duration (even; power play), action outcome (even: goals, penalties; counterattack: goals, no goal shots, penalties; power play: goals, no goal shots; transition: lost possessions), origin (even: zone 2, zone 4; counterattack: zone 5) and execution (even: free throws, drive shots; counterattack: drive shots, shots after > 2 fakes; power play: drive shots, shots after 1 fake) of shots; offensive even arrangements (2 vs. 2/1 vs. 1), and role; and defensive even arrangements (pressing, zones 1-2, zone M, zones 2-3-4). Differences between close and unbalanced games underlining that water polo performance should be analyzed in relation to specific margins of victory. Therefore, water polo coaches and physical trainers can plan a sound training session according to the most important aspects of close (opponent's exclusion, center forward play, and power play actions) and unbalanced (defensive skills) games.

Technical-tactical analysis of youth olympic taekwondo combat.

The purpose of this study was to define the technical and tactical profiles of official youth taekwondo competitions played under the most recent rules of the International Taekwondo Federation. Tactical actions (i.e., attack, defense, and block), technical executions (from 1- to 4-point scores), kicking legs (i.e., front/rear and right/left), and overall technical effectiveness were investigated in relation to match outcome of semifinal and final competitions (n = 50) of youth (aged 13-14 years) black belt athletes during the Italian Taekwondo Cadet Championship. Differences (p < 0.001) were found among all action typologies (Attack: 50.9 ± 2.2%; Defense: 27.7 ± 1.5%; Block: 21.3 ± 1.6%), with winners showing fewer (p = 0.005) offensive actions and more (p = 0.001) defensive actions with respect to non-winners. Independently from match outcome, technical exchanges showed differences (p < 0.001) for technical executions. Winners resulted more efficient (p < 0.001) for both technical and tactical variables. In general, these findings showed that Cadets tend to adopt an offensive strategy. In considering that the adoption of the new electronic system requires athletes to execute correct technical actions to have a score assigned, coaches should emphasize the effectiveness of scoring techniques and help athletes to effectively improve their defense and counterattack capabilities.

A simplified approach for estimating the ventilatory and respiratory compensation thresholds.

This study aimed to investigate whether ventilatory (VT) and respiratory compensation (RCT) thresholds could be derived from percentages of maximal running speed (Vmax). During the model building phase (1), VT & RCT of 31 competitive level athletes were identified with respiratory gas exchange. During the cross-validation phase (2), 20 subjects performed a treadmill test to identify Vmax and then they performed 30-min runs at velocities 2SE below or above the velocity at VT and RCT derived from (1), with measurement of blood lactate [BL], RPE, heart rate (HR), and speech comfort. Phase (1) revealed that VT and RCT were reached at 67 ± 9% and 84 ± 6% of Vmax. In (2) sustained running 2SE below VT (64% Vmax) was associated with the ability to finish 30-min, with low and constant [BL] (~2.5 mmol.l(-1)), moderate RPE (~3.0-3.5), a small HR drift, and ability to speak comfortably. Conversely, running at 2SE above RCT (86% Vmax) was associated with the inability to finish 30-min (18.5 ± 2.5 min to fatigue), increasing [BL] (end-exercise = 11.9 ± 0.9 mmol.l(-1)), high RPE (end-exercise = 8.9 ± 1.0), large HR drift (end-exercise = 98 ± 3% HRmax), and inability to speak comfortably. Simple percentages of Vmax (≤64% and ≥86%) obtained from a treadmill test without gas exchange, may be useful for prescribing exercise training intensities. Key pointsSimple performance parameters can be used to provide indications of physiologic thresholds.64% and 86% of the maximal running speed produce conditions consistent with ≤VT and ≥RCT.The combination of technology free feedback techniques such as the Talk Test and RPE and the simple %Vmax can be used as available and easy methods for the performance evaluation.Training prescription can be better addressed to the improvement of the aerobic or anaerobic capacity.

Does ergogenic effect of caffeine supplementation depend on CYP1A2 genotypes? A systematic review with meta-analysis.

BACKGROUND: The ergogenic effects of caffeine intake on exercise performance are well-established, even if differences exist among individuals in response to caffeine intake. The genetic variation of a specific gene, human cytochrome P450 enzyme 1A2 (CYP1A2) (rs762551), may be one reason for this difference. This systematic review and meta-analysis aimed to comprehensively evaluate the influence of CYP1A2 gene types on athletes' exercise performance after caffeine intake. METHODS: A literature search through 4 databases (Web of Science, PubMed, Scopus, and China National Knowledge Infrastructure) was conducted until March 2023. The effect size was expressed as the weighted mean difference (WMD) by calculating fixed effects meta-analysis if heterogeneity was not significant (I2 ≤ 50% and p ≥ 0.1). Subgroup analyses were performed based on AA and AC/CC genotype of CYP1A2. RESULTS: The final number of studies meeting the inclusion criteria was 12 (n = 666 participants). The overall analysis showed that the cycling time trial significantly improved after caffeine intake (WMD = -0.48, 95% confidence interval (95%CI): -0.83 to -0.13, p = 0.007). In subgroup analyses, acute caffeine intake improved cycling time trial only in individuals with the A allele (WMD = -0.90, 95%CI: -1.48 to -0.33, p = 0.002), but not the C allele (WMD = -0.08, 95%CI: -0.32 to 0.17, p = 0.53). Caffeine supplementation did not influence the Wingate (WMD = 8.07, 95%CI: -22.04 to 38.18, p = 0.60) or countermovement jump test (CMJ) performance (WMD = 1.17, 95%CI: -0.02 to 2.36, p = 0.05), and these outcomes were not influenced by CYP1A2 genotype. CONCLUSION: Participants with the CYP1A2 genotype with A allele improved their cycling time trials after caffeine supplementation. However, compared to placebo, acute caffeine supplementation failed to increase the Wingate or CMJ performance, regardless of CYP1A2 genotype.

Notational Analysis of Wheelchair Paralympic Table Tennis Matches.

Paralympic table tennis is the third largest paralympic sport for the number of players. Performance analysis was conducted for the rally duration and interval and impact of serve, whilst none investigated the shots distribution among classes of physical impairment. Therefore, the purpose of this study was to conduct a notational analysis of international competitions in relation to the wheelchair classes. Five matches for each wheelchair class (C1-to-C5) were evaluated from 20 elite male right-handed players. Both players for each match were analyzed for the following performance indicators: strokes type, the area of ball bouncing, and the shots outcome. Backhand shots were the most used technique for all classes. The most used strokes for C1 players were backhand and forehand drive and backhand lob, while for C5 players they were backhand and forehand push and backhand topspin. Similar shots distribution was registered for C2-to-C5 players. The central and far-from-the-net zone was mainly reached by the serve for all classes. Errors shots were similar in all classes, whilst winning shots were more frequent in C1. The current notational analysis provided a meaningful performance modelling of indicators for coaches and athletes that can be used to design training programs for each class.

Pax7+ Satellite Cells in Human Skeletal Muscle After Exercise: A Systematic Review and Meta-analysis.

BACKGROUND: Skeletal muscle has extraordinary regenerative capabilities against challenge, mainly owing to its resident muscle stem cells, commonly identified by Pax7+, which expediently donate nuclei to the regenerating multinucleated myofibers. This local reserve of stem cells in damaged muscle tissues is replenished by undifferentiated bone marrow stem cells (CD34+) permeating into the surrounding vascular system. OBJECTIVE: The purpose of the study was to provide a quantitative estimate for the changes in Pax7+ muscle stem cells (satellite cells) in humans following an acute bout of exercise until 96 h, in temporal relation to circulating CD34+ bone marrow stem cells. A subgroup analysis of age was also performed. METHODS: Four databases (Web of Science, PubMed, Scopus, and BASE) were used for the literature search until February 2022. Pax7+ cells in human skeletal muscle were the primary outcome. Circulating CD34+ cells were the secondary outcome. The standardized mean difference (SMD) was calculated using a random-effects meta-analysis. Subgroup analyses were conducted to examine the influence of age, training status, type of exercise, and follow-up time after exercise. RESULTS: The final search identified 20 studies for Pax7+ cells comprising a total of 370 participants between the average age of 21 and 74 years and 26 studies for circulating CD34+ bone marrow stem cells comprising 494 participants between the average age of 21 and 67 years. Only one study assessed Pax7+ cells immediately after aerobic exercise and showed a 32% reduction in exercising muscle followed by a fast repletion to pre-exercise level within 3 h. A large effect on increasing Pax7+ cell content in skeletal muscles was observed 24 h after resistance exercise (SMD = 0.89, p < 0.001). Pax7+ cells increased to ~ 50% above pre-exercise level 24-72 h after resistance exercise. For a subgroup analysis of age, a large effect (SMD = 0.81, p < 0.001) was observed on increasing Pax7+ cells in exercised muscle among adults aged > 50 years, whereas adults at younger age presented a medium effect (SMD = 0.64, p < 0.001). Both resistance exercise and aerobic exercise showed a medium overall effect in increasing circulating CD34+ cells (SMD = 0.53, p < 0.001), which declined quickly to the pre-exercise baseline level after exercise within 6 h. CONCLUSIONS: An immediate depletion of Pax7+ cells in exercising skeletal muscle concurrent with a transient release of CD34+ cells suggest a replenishment of the local stem cell reserve from bone marrow. A protracted Pax7+ cell expansion in the muscle can be observed during 24-72 h after resistance exercise. This result provides a scientific basis for exercise recommendations on weekly cycles allowing for adequate recovery time. Exercise-induced Pax7+ cell expansion in muscle remains significant at higher age, despite a lower stem cell reserve after age 50 years. More studies are required to confirm whether Pax7+ cell increment can occur after aerobic exercise. CLINICAL TRIAL REGISTRATION: Registered at the International Prospective Register of Systematic Reviews (PROSPERO) [identification code CRD42021265457].

Interplay between Protein Kinase C Epsilon and Reactive Oxygen Species during Myogenic Differentiation.

Reactive oxygen species (ROS) are currently recognized as a key driver of several physiological processes. Increasing evidence indicates that ROS levels can affect myogenic differentiation, but the molecular mechanisms still need to be elucidated. Protein kinase C (PKC) epsilon (PKCe) promotes muscle stem cell differentiation and regeneration of skeletal muscle after injury. PKCs play a tissue-specific role in redox biology, with specific isoforms being both a target of ROS and an up-stream regulator of ROS production. Therefore, we hypothesized that PKCe represents a molecular link between redox homeostasis and myogenic differentiation. We used an in vitro model of a mouse myoblast cell line (C2C12) to study the PKC-redox axis. We demonstrated that the transition from a myoblast to myotube is typified by increased PKCe protein content and decreased ROS. Intriguingly, the expression of the antioxidant enzyme superoxide dismutase 2 (SOD2) is significantly higher in the late phases of myogenic differentiation, mimicking PKCe protein content. Furthermore, we demonstrated that PKCe inhibition increases ROS and reduces SOD2 protein content while SOD2 silencing did not affect PKCe protein content, suggesting that the kinase could be an up-stream regulator of SOD2. To support this hypothesis, we found that in C2C12 cells, PKCe interacts with Nrf2, whose activation induces SOD2 transcription. Overall, our results indicate that PKCe is capable of activating the antioxidant signaling preventing ROS accumulation in a myotube, eventually promoting myogenic differentiation.

How divergent are children's divergent movements? The role of cognition and expertise in a class-randomized cross-over trial.

This study aimed at investigating (1) the cognitive and motor predictors of divergent movement ability (DMA) in childhood and (2) the role of sport and enriched physical education (PE) experience. Participants were 165 fifth graders, aged 10-11 years, with different histories (onset and duration) of participation in enriched PE. They were assessed in cognitive/attentional and motor/sport skills and active play/sport habits at baseline, and six months later in DMA. Results of regression analyses showed an overall prediction of DMA by sport practice and a nuanced prediction of individual DMA indices (fluency, flexibility, originality) by decision making and spatial attention ability. Linear mixed models revealed better DMA in children exposed to enriched PE, with differential effects on DMA indices depending on its duration and earlier/later onset. The results identify novel cognitive determinants of children's DMA and suggest that sport practice and experience of designed enrichment in PE may benefit DMA.

Defecation enhances cerebral perfusion and delays fatigue in elite triathletes.

BACKGROUND: Rectal distension increases regulatory burden to autonomic nervous system in the brain. PURPOSE: To determine the effect of rectal defecation on endurance performance and blood supply to the prefrontal brain and sub-navel regions of elite triathletes. METHODS: Thirteen elite triathletes completed a cycling time trial (80% VO2max) under defecated and non-defecated conditions, using a counterbalanced crossover design. Oxygenation and blood distribution in prefrontal brain and sub-navel regions were monitored by near-infrared spectroscopy (NIRS) during cycling. RESULTS: Defecation moderately decreased systolic blood pressure (-4 mmHg, p < 0.05, d = 0.71), suggesting an alleviation of autonomic nervous activity. During the exercise trials, fatigue (cycling time to exhaustion) occurred when cerebral oxygenation decreased to ~ 5 % below baseline regardless of treatment conditions, suggesting a critical deoxygenation point for sustaining voluntary exertions. Cerebral blood (estimated by total hemoglobin) increased progressively throughout the entire exercise period. Defecation decreased sub-navel oxygenation levels below the non-defecated level, suggesting an increased sub-navel oxygen consumption. Exercise also decreased sub-navel blood distribution, with minimal difference between non-defecated and defecated conditions. Defecation improved blood pooling in the prefrontal brain during exercise (p < 0.05) and enhanced cycling performance in triathletes (Non-defecated: 1624 ± 138 s vs. defecated: 1902 ± 163 s, d = 0.51, p < 0.05). CONCLUSION: Our results suggest that improved exercise performance after defecation is associated with greater blood availability to compensate deoxygenation in the prefrontal brain region during exercise. Further investigation is needed to examine the role of increasing sub-navel oxygen consumption in the performance improvement after defecation.