Optimization of constructed wetlands on purifying black-odorous water and their potential purification mechanism.

Black-odorous water has become a common and widespread problem in recent decades. In this study, nine constructed wetlands (CWs) with different flow types, filters, plants, and hydraulic loadings were designed according to an orthogonal array (L9 (34), and were used for the purification of black-odorous water in summer and winter. The results showed that CWs are regarded as effective to purify black-odorous water in both seasons. Microbial degradation is the major removal pathway of pollutants in CWs during summer, while the joint effect of biodegradation and adsorption is the main treatment route during winter. Flow type and hydraulic loading appear to be the most important factors impacting the purification performance of CWs, by changing the redox condition of systems and retention time of contaminants, respectively. 'Vertical flow-zeolite filter-high loading' is proposed as the best parameter selection for CWs on the purification of black-odorous water: among them, CWs with vertical flow have better oxygen transport capacity that is conductive to aerobic processes of pollutants, zeolite substrates may adsorb more nitrogen via ion exchange, higher hydraulic loadings can extend the contact time between contaminants and filters, and regulate the water temperature for microbial activity.

Removal efficiency, kinetic, and behavior of antibiotics from sewage treatment plant effluent in a hybrid constructed wetland and a layered biological filter.

Sewage treatment plant (STP) is the major point source of antibiotic contamination, yet the advanced treatment of antibiotic polluted STP effluent has not been given necessary attention. This study is conducted to evaluate the removal efficiency, kinetic, and behavior of sulfonamides, quinolones, tetracyclines, and macrolides antibiotics from STP effluent in a hybrid constructed wetland (HCW) and a layered biological filter (LBF) at different hydraulic loading rates (HLRs). The results showed that the removal efficiency of antibiotics in all the HLRs was ranked as follow: quinolones of HCW (70-95%) > macrolides of HCW (58-77%) > tetracyclines of both systems (59-67%) > quinolones of LBF (28-64%) > macrolides of LBF (13-25%) > sulfonamides of both systems (<0%). The optimal HLR is 1.0 m/day for quinolones and 2.0 m/day for tetracyclines-macrolides in the HCW, and 6.4 m/day for quinolones-tetracyclines in the LBF, respectively. Although HCW performed better on the removal of most antibiotics, LBF exhibited stronger total loading toleration and higher removal loading ability to antibiotics. Among them, quinolones were markedly removed by multiple effect of substrate adsorption, microbial anaerobic degradation, and photolysis in the HCW (planted), and by filter sorption and interception in the LBF (unplanted); adsorption is the dominant elimination approach for tetracyclines in both systems; plant uptake plays a significant role on the removal of macrolides in the HCW.

[Mechanisms of total flavones of Epimedium on oxidative stress induced by myocardial ischemia/reperfusion injury in rats].

To investigate the effects and mechanisms of total flavones of Epimedium (TFE) on oxidative stress induced by myocardial ischemia/reperfusion injury in rats, forty male SD rats were randomly divided into sham operated group, model group, diltiazem group and flavonoids of Epimedium low and high doses groups with 8 rats in each. Myocardial ischemia/reperfusion injury model was induced by ligaturing the left anterior descending artery for 30 min followed reperfusion for 4 h after TFE was taken by intragastric administration for 4 days. The degree of myocardial infarct was observed by N-BT staining. The concentrations of MDA and activities of SOD and T-AOC in cardiac tissue were measured by colorimetry. Serum TnI concentrations were checked by ELISA. HE stain was used to observe myocardium structure under light microscope. Expressions of SIRT1 and Nrf2 in cardiac tissue were evaluated by immunohistochemistry method and Western blot, respectively. Compared with the model group, the degree of myocardial infarct, MDA concentration in cardiac tissue and the levels of TnI in serum significantly decreased in the diltiazem group and flavonoids of Epimedium low and high doses groups (P<0.05 or P<0.01); flavonoids of Epimedium low and high doses groups and the diltiazem group also showed improvements in myocardium structure under ischemia/reperfusion injury. TFE significantly increased the activity of SOD and T-AOC and the expression of SIRT1 and Nrf2 in cardiac tissue when compared with the model group (P<0.05 or P<0.01). Therefore, TFE can increase anti-peroxidant capacity of myocardium tissue by using intrinsically anti-oxidant signaling pathway of SIRT1 and Nrf2, which can inhibit irreversible damage of cardiomyocytes in myocardial ischemia/reperfusion injury and protect normal function of cardiac tissue.

miR-590-3p Is a Novel MicroRNA in Myocarditis by Targeting Nuclear Factor Kappa-B in vivo.

OBJECTIVE: Nuclear factor kappa-B (NF-954;B)-induced inflammation leads to myocarditis and heart dysfunction. How microRNAs (miRNAs) contribute to this process is poorly defined. The aim of this study was to investigate whether miRNAs regulate NF-954;B-induced inflammation in experimental autoimmune myocarditis (EAM) in vivo. METHODS AND RESULTS: NF-954;B and its related proinflammatory genes, including interleukin-6 (IL-6) and tumor necrosis factor-a (TNF-a), were activated in EAM. Profiling of NF-954;B-related miRNAs revealed that miR-590-3p was strikingly reduced in EAM. We found IL-6-induced proinflammatory signaling via miR-590-3p reduction, p50 induction, NF-954;B activation and IL-6/TNF-a expression. Moreover, a luciferase reporter assay demonstrated that miR-590-3p directly interacted with the 3' UTR (untranslated region) of the p50 subunit, and that miR-590-3p overexpression inhibited p50 expression. Finally, miR-590-3p transfection through adeno-associated virus significantly inhibited p50 expression, suppressed NF-954;B activity and blocked IL-6/TNF-a expression in vivo, reducing the lesion area and improving cardiac function in EAM. CONCLUSION: miR-590-3p is a novel NF-954;B-related miRNA that directly targets the p50 subunit. This may provide a novel strategy for the treatment of myocarditis.

Purification of the secondary treatment tail water for wastewater reclamation by integrated subsurface-constructed wetlands.

A whole-year investigation of full-scale integrated subsurface-constructed wetlands (ISCWs) was carried out to purify the tail water from a wastewater treatment plant (WWTP) for wastewater reclamation under four plant species, four hydraulic loading rates (HLRs), and four seasons. The results showed that ISCWs were effective for the purification of WWTP discharge, with the average removal efficiencies of COD, NH4+-N, TN, and TP being 48%, 49%, 9%, and 30%, respectively. Typical pollutant concentrations in the treated effluent of ISCWs were 8.19 mg/L COD, 1.76 mg/L NH4+-N, 11.57 mg/L TN, and 0.36 mg/L TP, which met most of the water quality standards for reusing recycling water. Emergent plants with well-developed root systems may be capable of promoting the decontamination of ISCWs. Seasonal change played an important role in the treatment process: the removal of phosphorus by plant uptake and microbial utilization was more active in the warm season and the co-occurrence of organic degradation and nitrification, whereas the cold season is conducive to exothermic adsorption process of pollutants to substrates. Properly increasing the HLRs may improve the availability of ISCWs according to the requirement of effluent quality. Furthermore, the C/N ratio might be the key factor for the purification effect of ISCWs, because the COD level of WWTP discharge may change the process of NH4+-N biotransformation.

Evaluation method of ore grade estimation effectiveness.

This study proposes a new method to evaluate the effectiveness of orebody grade estimations, drawing upon the analysis of existing evaluation methods for grade estimation. This new approach addresses factors such as uneven sampling and asymmetric estimation range, which are challenging to overcome with existing evaluation techniques. The core principle of this method involves documenting how frequently individual samples are used during grade estimation and calculating the total distance weights for each sample. Subsequently, the usage frequency and total weight of the samples are standardized, and these standardized values are weighted based on the sample grades. A comparison is made between the weighted sample grades and the estimated grades, with the closeness between the two serving as a metric for assessing the effectiveness of the estimation. This study compares the new evaluation method to the direct comparison and cross-validation methods, examining the effectiveness of grade estimation using the inverse distance weighting (IDW) method. The findings revealed that: (1) The new evaluation method theoretically accounts for the systematic deviation between the statistical measures of estimated and sample grades resulting from uneven sample distribution, offering a fresh approach for enhancing the effectiveness of orebody grade estimation. (2) In the grade estimation of experimental Fe samples, the frequency of usage and the sum of distance weights were unequal. This inequality significantly contributes to the systematic deviation between the estimated and sample grades. (3) Comparing the new evaluation method to others confirms the stability and reliability of the new approach for evaluating the effectiveness of orebody grade estimation. This novel method demonstrates theoretical advantages and practical utility. (4) The deviation between the estimated grades and the statistical results of sample grades is influenced by the distribution pattern of sample grades, the spatial relationship between samples and estimation blocks, and the inherent systematic error associated with the IDW method. This systematic error cannot be overlooked.

A three-year study on the treatment of domestic-industrial mixed wastewater using a full-scale hybrid constructed wetland.

Three full-scale constructed wetlands (CWs), namely vertical flow (VFCW), surface flow (SFCW), and horizontal flow (HFCW) systems, were combined in a series process to form a hybrid CW, which was used for the treatment performance of domestic-industrial mixed wastewater and investigated over a three-year period. The hybrid CW demonstrated that it is effective and stable during the long-term treatment of high-loading mixed wastewater under different operation years, season changes, and technology processes, with the average removal efficiencies of suspended solids, chemical oxygen demand, biological oxygen demand, total nitrogen, ammonia nitrogen, nitrate nitrogen, and total phosphorous being 84, 40, 54, 54, 70, 40, and 46%, respectively. The effluent quality of the hybrid CW reached the highest discharge standard for wastewater treatment plants. First, a variety of pollutants from the mixed wastewater were effectively removed in the subsurface processes (VFCW and HFCW) via substrate adsorption and degradation of the attached biofilm. The higher dissolved oxygen content and oxygen transfer capacity values in the VFCW were favourable for the occurrence of aerobic pathways (such as nitrification and inorganic phosphorus oxidation). In addition, with the large consumption of oxygen in the previous process, the oxygen-enriching capacity of the SFCW processes, provided aerobic potential for the next stage. In particular, the plant debris in the SFCW temporarily increased the organics and suspended solids, further increasing the C/N ratio, which was beneficial for denitrification as the main nitrogen removal pathway in the HFCW.

A new method for estimating ore grade based on sample length weighting.

Estimation of ore grade is very important for the value evaluation of ore deposits, and it directly affects the development of mineral resources. To improve the accuracy of the inverse distance weighting (IDW) method in ore grade estimation and reduce the smoothing effect of the IDW method in grade estimation, the weight calculation method involved in the IDW method was improved. The length parameter of the ore sample was used to calculate the weight of the IDW method. The length of the ore samples was used as a new factor of the weighting calculation. A new method of IDW integrated with sample length weighting (IDWW) was proposed. The grade estimation of Li, Al, and Fe in porcelain clay ore was used as a case study. A comparative protocol for grade estimation via the IDWW method was designed and implemented. The number of samples involved in the estimation, sample combination, sample grade distribution, and other factors affecting the grade estimation were considered in the experimental scheme. The grade estimation results of the IDWW and the IDW methods were used for comparative analysis of grades of the original and combined samples. The estimated results of the IDWW method were also compared with those of the IDW method. The deviation analysis of the estimated grade mainly included the minimum, maximum, mean, and coefficient of variation of the ore grade. The estimation effect of IDWW method was verified. The minimum deviations of the estimated grade of Li, Al, and Fe were between 9.129% and 59.554%. The maximum deviations were between 4.210 and 22.375%. The mean deviations were between - 1.068 and 7.187%. The deviations in the coefficient of variation were between 3.076 and 36.186%. The deviations in the maximum, minimum, mean, and coefficients of variation of the IDWW were consistent with those of the IDW, demonstrating the accuracy and stability of the IDWW method. The more the samples involved in the estimation, the greater the estimation deviations of IDW and IDWW methods. The estimated deviations of Li, Al, and Fe were affected by the shape of the grade distribution, when the same estimation parameters were used. The grade distribution pattern of the samples significantly influenced the grade estimation results. The IDWW method offers significant theoretical advantages and addresses the adverse effects of uneven sample lengths on the estimates. The IDWW method can effectively reduce the smoothing effect and improves the utilization efficiency of the original samples.

Cytotoxic cis-ruthenium(III) bis(amidine) complexes.

In chemotherapy, the search for ruthenium compounds as alternatives to platinum compounds is proposed because of their unique properties. However, the geometry effect of ruthenium complexes is sparely investigated. In this paper, we report the synthesis of a series of bis(acetylacetonato)ruthenium(III) complexes bearing two amidines (1-) in a cis configuration. These complexes are highly cytotoxic against various cancer cell lines, including a cisplatin-resistant cell line. In vitro studies suggested that the representative complex can induce cell cycle G0/G1 phase arrest, decrease the mitochondrial membrane potential, elevate the intracellular reactive oxygen species level, and cause DNA damage and caspase-mediated mitochondrial pathway apoptosis in NCI-H460 cells. In vivo, it can effectively inhibit tumor xenograft growth in nude mouse models with no body weight loss. In combination with the reported trans-bis(amidine)ruthenium(III) complexes, we found that ruthenium(III) bis(amidine) complexes could be cytotoxic in both trans and cis geometries, which is in contrast to platinum-based compounds.

Associations between coagulation factor XII, coagulation factor XI, and stability of venous thromboembolism: A case-control study.

BACKGROUND: Pulmonary embolism (PE) is a fatal clinical syndrome that is generally caused by an embolus from unstable deep venous thrombosis (DVT). However, clinical and biochemical factors that are related to the stability of DVT are not fully understood. AIM: To evaluate the relationships between plasma antigen levels of factor XII (FXII:Ag) and factor XI (FXI:Ag) with the stability of DVT. METHODS: Patients with DVT and no PE, DVT and PE, and controls with no DVT or PE that matched for age, gender, and comorbidities were included in this study. FXII:Ag and FXI:Ag in peripheral venous blood were measured using enzyme-linked immunosorbent assays. RESULTS: Using the 95th percentile of FXI:Ag in patients with DVT and PE as the cut-off, a higher FXI:Ag was associated with a higher risk of unstable DVT (odds ratio: 3.15, 95% confidence interval: 1.18-8.43, P = 0.019). Stratified analyses showed consistent results in patients ≤ 60 years (P = 0.020), but not in those > 60 years (P = 0.346). CONCLUSION: Higher plasma FXI:Ag might be a marker for unstable DVT, which might be associated with PE in these patients.

Metformin ameliorates the proinflammatory state in patients with carotid artery atherosclerosis through sirtuin 1 induction.

Metformin is a widely used classic antidiabetic drug. However, its clinical pharmacologic mechanism remains poorly understood. In the present study, we investigated the anti-inflammatory effects of metformin on circulating peripheral blood mononuclear cells (MNCs) of patients with carotid artery atherosclerosis (AS). A total of 42 patients with carotid artery AS were randomly assigned to metformin (500 mg twice a day; Met; n = 21) or placebo control (Con; n = 21) groups. After 12 weeks of treatment, plasma concentrations of high-sensitivity C-reactive protein (hs-CRP), interleukin 6 (IL-6), and tumor necrosis factor α (TNF-α) significantly decreased in the Met group compared with the Con group. In addition, treatment with metformin significantly reduced the expression of IL-6 and TNF-α at the messenger RNA level and attenuated nuclear factor kappa B (NF-κB) DNA binding activity in MNCs. Intriguingly, metformin did not alter the expression of NF-κB p65 subunit, but markedly inhibited its acetylation. Furthermore, metformin significantly induced sirtuin 1 (SIRT1) expression in MNCs. Moreover, we found that metformin treatment dramatically induced SIRT1 expression, blocked p65 acetylation, and inhibited NF-κB activity and the expression of inflammatory factors in MNCs in vitro. We conclude that metformin has a novel direct protective role to ameliorate the proinflammatory response through SIRT1 induction, p65 acetylation reduction, NF-κB inactivation, and inflammatory inhibition in peripheral blood MNCs of patients with carotid artery AS.