[Study on lung injury induced by rare earth samarium oxide particles in rats].

Objective: To study the effect of samarium trioxide (Sm(2)O(3)) particles on rat lung tissue and compare it with the same dose of silica (SiO(2)) particles, in order to find the reference index for early screening of pneumoconiosis. Methods: In October 2018, 72 SPF healthy male rats were randomly divided into control group, SiO(2) group and Sm(2)O(3) group. The lungs of rats in each group were perfused with 2.0 ml/kg normal saline and 280 mg/kg SiO(2) and Sm(2)O(3) particle suspension by one-time non exposed tracheal perfusion. The lungs of rats were stained with hematoxylin eosin (HE) staining, and the pathological changes of lung tissues were observed. The concentrations of SNAIL homologue 1 (SNAI1) , SNAIL homologue 2 (SNAI2) , and heat shock protein-27 (HSP-27) in rat serum were detected by enzyme-linked immunosorbent assay. 0.5 g of lung tissue from rats in Sm(2)O(3) group and control group exposed to dust for 56 days was screened for long-chain noncoding RNA (lncRNA) and circular RNA (circRNA) . Results: After 7 days of dust exposure, the alveoli in SiO(2) group and Sm(2)O(3) group were disordered, and lymphoid tissue aggregation and proliferation were observed around the bronchial wall. At 14 days, a large number of lymphocytes infiltrated in SiO(2) group, and a small number of macrophages containing Sm(2)O(3) and fibrotic nodules scattered in Sm(2)O(3) group. At 28 days, a small amount of lymphocyte infiltration appeared in SiO(2) group, and fibrotic nodules were seen in some areas of Sm(2)O(3) group. At 56 days, there was a small amount of fibroblast proliferation in SiO(2) group, and a large number of fibrotic nodules containing gray black matter were seen in Sm(2)O(3) group. There was no significant difference in lung organ coefficient among groups at different dust exposure time (P>0.05) . After 14 days of dust exposure, the contents of SNAI1 and SNAI2 in serum of rats in SiO(2) group were lower than those in control group, the content of SNAI2 in serum of Sm(2)O(3) group was lower than that in control group, and the contents of SNAI1 and SNAI2 in serum of Sm(2)O(3) group were higher than those in SiO(2) group (P<0.05) . The content of HSP-27 in SiO(2) group was lower than that in control group (P<0.05) . After 56 days of dust exposure, the content of HSP-27 in Sm(2)O(3) group was lower than that in control group (P<0.05) . At 56 days, lncRNA in Sm(2)O(3) group was up-regulated by 148 and down regulated by 725, circRNA was up-regulated by 16 and down regulated by 153. Conclusion: Sm(2)O(3) can cause lung injury in rats, and the change of SNAI2 content can be detected in the early stage, which can be used as a reference index for early screening of pneumoconiosis. There are differences in the expression of lncRNA and circRNA after 56 days of dust exposure in rats, which may be related to the pathogenesis of pneumoconiosis.

[Analysis on duplicated reporting of brucellosis in China, 2005-2017].

Objective: To analyze the current status of duplicated reporting of brucellosis in China during 2005-2017, to understand the impact of duplicated reporting on the incidence of brucellosis, analyze the possible causes of duplicated reporting, and put forward propose solutions to further improve the brucellosis surveillance data quality. Methods: Data were from brucellosis case reporting cards in National Notifiable Disease Report System (NNDRS), Excel 2010 software was used to establish the database, the duplicated reporting was identified by using Spark DataFrame aggregation analysis in Python 3.2. Results: During 2005-2017, a total of 499 577 cases of brucellosis were reported, but the duplicated reporting rate was 75.76/10 000 (3 785/499 577). The duplicated reporting rate in same year was 37.21/10 000 (1 859/499 577), the duplicated reporting rate in other year was 39.87/10 000 (1 992/499 577) and the overall duplicated reporting rate increased year by year. The duplicated reporting rate in same institution was 33.59% (1 271/3 785) and the duplicated reporting rate by other institutions was 66.41% (2 514/3 785). Hospitals had the highest number of duplicated reporting (2 757), and community healthcare centers had the highest of duplicated reporting rate (281.27/10 000) (123/4 373). The reported incidence of brucellosis was 2.926 4/100 000 in 2017, the corrected incidence was 2.844 8/100 000 after removing the duplicated reporting, which was the maximum decrease from 2005 to 2017, the corrected incidence decreased by 2.79%. From 2005 to 2017, the average reported incidence of brucellosis in Xinjiang Uygur autonomous region was 14.712 0/100 000, and the corrected incidence was 14.060 0/100 000 after removing the duplicate reporting, with a decrease by 4.43%, the biggest decline in China. The average reported incidence of brucellosis in Ningxia Hui autonomous region was 13.987 1/100 000, and the corrected incidence of brucellosis was 13.662 2/100 000, with a decrease of 2.32%, ranking second in China. The reasons for duplicated reporting of brucellosis included repeated medical care seeking in same or different medical institutions in same year or other year, including the repeated medical care seeking in other areas. Conclusions: During 2005-2017, the number of duplicated reporting of brucellosis cases was on the rise in China. Hospital and community health service centers had highest case number and rate of duplicate reporting respectively, the duplicated reporting was mainly caused by re-reporting by other institutions or in other years. The duplicated reporting of brucellosis had greatly affected the accuracy of surveillance data of brucellosis in some provinces, such as Xinjiang and Ningxia. It is recommended to further strengthen brucellosis reporting management, improve data quality of NNDRS.

Enhancement of a two-photon absorption cross section (TPACS)--design and synthesis of a novel class of photochromic molecules with large TPACS.

Two novel photochromic compounds were designed and synthesized; the two-photon absorption (TPA) cross section of both were measured with a nanosecond laser pulse; their TPACS (delta) values are around 25 x 10(-46) cm4 s photon-1 molecule-1 in acetonitrile; the molecular structure of the target compounds have a 3-methyl-1-benzothiophen-2-yl moiety, which can greatly enhance the two-photon absorption cross section.