RESUMO
PURPOSE: Identifying actionable oncogenic mutations have changed the therapeutic landscape in different types of tumors. This study investigated the utility of comprehensive genomic profiling (CGP), a hybrid capture-based next-generation sequencing (NGS) assay, in clinical practice in a developing country. METHODS: In this retrospective cohort study, CGP was performed on clinical samples from patients with different solid tumors recruited between December 2016 and November 2020, using hybrid capture-based genomic profiling, at the individual treating physicians' request in the clinical care for therapy decisions. Kaplan-Meier survival curves were estimated to characterize the time-to-event variables. RESULTS: Patients median age was 61 years (range: 14-87 years), and 64.7% were female. The most common histological diagnosis was lung primary tumors, with 90 patients corresponding to 52.9% of the samples (95% CI 45.4-60.4%). Actionable mutations with FDA-approved medications for specific alterations correspondent to tumoral histology were identified in 58 cases (46.4%), whereas other alterations were detected in 47 different samples (37.6%). The median overall survival was 15.5 months (95% CI 11.7 months-NR). Patients who were subjected to genomic evaluation at diagnosis reached a median overall survival of 18.3 months (95% CI 14.9 months-NR) compared to 14.1 months (95% CI 11.1 months-NR) in patients who obtained genomic evaluation after tumor progression and during standard treatment (P = .7). CONCLUSION: CGP of different types of tumors identifies clinically relevant genomic alterations that have benefited from targeted therapy and improve cancer care in a developing country to guide personalized treatment to beneficial outcomes of cancer patients.
Assuntos
Países em Desenvolvimento , Neoplasias Pulmonares , Humanos , Feminino , Pessoa de Meia-Idade , Masculino , Estudos Retrospectivos , Neoplasias Pulmonares/patologia , Mutação , Genômica , Sequenciamento de Nucleotídeos em Larga EscalaRESUMO
ABSTRACT Caiman zoo breeding (Caiman crocodilus fuscus) has been developing with greater force in Colombia since the 90s. It is essential to evaluate the physiological ranges of the species to be able to assess those situations in which their health is threatened. The objective of the present study was to determine the typical hematological values of the Caiman (Caiman crocodilus fuscus) with the aid of the microhematocrit, the cyanmethemoglobin technique, and a hematological analyzer. The blood samples were taken from 120 young animals of both sexes in good health apparently (males 44 and females 76). The samples were taken from the coccygeal vein between the second and third interscalar space with lilac lid vacutainer tube whit acid ethylene-diamine-tetra-acetic as an anticoagulant. The animals are located in the middle of the Magdalena area in Colombia. The results obtained on average were Hematocrit: 23.36 %, Hemoglobin: 7.78 g/dl, Red blood cells: 3.89 106/µL, Average corpuscular volume: 60 fl, Average corpuscular hemoglobin concentration: 33.33 g/dl and Average corpuscular hemoglobin: 73.9 pg. This is the first study of hematological values for this particular species in Colombia.
RESUMEN La zoocría de babilla (Caiman crocodrilus fuscus) se ha venido desarrollando con mayor fuerza en Colombia desde los años 90. Es muy importante evaluar los rangos fisiológicos de la especie para poder valorar aquellas situaciones en las que su salud se vea comprometida. El objetivo del presente trabajo fue determinar los valores hematológicos normales del caimán común (Caimán crocodrilus fuscus) con la ayuda del microhematocrito, la técnica de cianometahemoglobina y un analizador hematológico. Las muestras de sangre se obtuvieron de 120 animales en aparente buen estado de salud, machos (44) y hembras (76); se tomaron de la vena coccígea entre el segundo y tercer espacio interescamal con tubo vacutainer tapa lila, con ácido etilen-diamino-tetra-acético como anticoagulante. Los animales están ubicados en la zona del Magdalena medio en Colombia, todos en cautiverio. Los resultados obtenidos en promedio fueron: Hematocrito: 23,36 %, Hemoglobina: 7,78 g/dl, Glóbulos rojos: 3,89 106/µL, Volumen corpuscular medio: 60 fl, Concentración de Hemoglobina corpuscular media: 33,33 g/dl y Hemoglobina corpuscular media: 73,9 pg. Este es el primer reporte de valores hematológicos para esta especie en Colombia y Latinoamérica.