Integrating bioelectrochemical system with aerobic bioreactor for organics removal and caustic recovery from alkaline saline wastewater.

Bioelectrochemical systems (BES) are increasingly being explored as an auxiliary unit process to enhance conventional waste treatment processes. This study proposed and validated the application of a dual-chamber bioelectrochemical cell as an add-on unit for an aerobic bioreactor to facilitate reagent-free pH-correction, organics removal and caustic recovery from an alkaline and saline wastewater. The process was continuously fed (hydraulic retention time (HRT) of 6 h) with a saline (25 g NaCl/L) and alkaline (pH 13) influent containing oxalate (25 mM) and acetate (25 mM) as the target organic impurities present in alumina refinery wastewater. Results suggested that the BES concurrently removed the majority of the influent organics and reduced the pH to a suitable range (9-9.5) for the aerobic bioreactor to further remove the residual organics. Compared to the aerobic bioreactor, the BES enabled a faster removal of oxalate (242 ± 27 vs. 100 ± 9.5 mg/L.h), whereas similar removal rates (93 ± 16 vs. 114 ± 23 mg/L.h, respectively) were recorded for acetate. Increasing catholyte HRT from 6 to 24 h increased the caustic strength from 0.22% to 0.86%. The BES enabled caustic production at an electrical energy demand of 0.47 kWh/kg-caustic, which is a fraction (22%) of the electrical energy requirement for caustic production using conventional chlor-alkali processes. The proposed application of BES holds promise to improve environmental sustainability of industries in managing organic impurities in alkaline and saline waste streams.

Long-term performance of a full-scale intermittently decanted extended aeration (IDEA) plant: The effect of dissolved oxygen and the relocation of alum dosing to bioselector.

Dosing alum to remove phosphorus (P) from wastewater is a common practice. However, the dosing-location and quantity of alum required to meet P discharge limits are vaguely defined. As such, utilities overdose alum to avoid noncompliance, but this leads to wastage and costs. This study aimed to address this issue through a long-term evaluation of an alum-assisted full-scale intermittently decanted extended aeration (IDEA) plant. Specifically, the effects of relocating alum dosing from a low P containing IDEA-tank to a bioselector containing elevated P concentrations were examined. The plant is fitted with two IDEA-tanks, each retrofitted with a bioselector at the inlet end. Over 359 d, key parameters (dissolved oxygen (DO), NH4+-N, NO2--N, NO3--N, PO43--P) were quantified to account for the effects of switching alum-dosing into the bioselector and varying dosages (429, 643, 1072 and 1286 g-Al3+ per treatment cycle). Results indicated a 52% reduction of alum usage with no impact on discharge limit (≤0.85 mg-P/L). As expected, a failure to maintain DO setpoint (1.6 mg/L) reduced both NH4+-N and PO43--P removal. Increasing alum dosage simply could not alleviate this problem, but maintenance of DO at least 85% of setpoint enabled effective rectification. This 15% DO buffer zone offers operators an opportunity to rectify imminent operational failures related to DO, prior to escalating alum dosage. An operational framework to manage DO related failures is proposed. Overall, this study offers insights on how to cost effectively apply alum and manage DO failures to achieve P discharge limits in IDEA plants.

Simultaneous nitrification, denitrification and phosphorus recovery (SNDPr) - An opportunity to facilitate full-scale recovery of phosphorus from municipal wastewater.

Sewage treatment plants are a potential point source for recycling of phosphorus (P). Several technologies have been proposed to biologically recover P from wastewater. The majority of these technologies are side-stream processes and rely on an external source of soluble organic carbon to facilitate P recovery. To date, no studies have demonstrated the potential to facilitate main-stream recovery of P, using carbon that is naturally present in wastewater. Simultaneous nitrification, denitrification and phosphorus removal (SNDPR) is an elegant process that can uptake influent carbon and effectively remove both nitrogen (N) and P from wastewater. SNDPR studies to date, however, have failed to facilitate an end-of-anaerobic-phase P rich liquor, that enables economies of scale to recover influent P. Therefore, this study examined the feasibility of achieving a P rich liquor (e.g. > 70 mg-P/L) in a granular SNDPR process. A synthetic influent that replicated the nutrient and carbon concentrations of municipal wastewater was used to investigate whether carbon in the influent wastewater could enable both nutrient removal and P recovery from wastewater. Our granular SNDPR process was able to facilitate an end-of-anaerobic-phase liquor with P enriched to approximately 100 mg-P/L. A dissolved oxygen (DO) concentration of 0.5 mg/L in a sequencing batch reactor (SBR) was found to be essential to achieve complete nutrient removal and a high P concentration at the end of the anaerobic phase. At this steady state of reactor operation, the abundance of polyphosphate accumulating organisms (PAOs) was 2.6 times the abundance of glycogen accumulating organisms (GAOs). The study also demonstrated the importance of denitrifying polyphosphate accumulating organisms (DPAOs) and glycogen accumulating organisms (DGAOs) to achieve complete removal of N from the effluent. Compared to nitrifying bacteria, the polyphosphate accumulating organisms (PAOs) had a higher affinity towards DO. This study, for the first time, showed that the mainstream recovery of P is feasible using a SNDPR process.

Sorptive removal of toluene and m-xylene by municipal solid waste biochar: Simultaneous municipal solid waste management and remediation of volatile organic compounds.

The remediation of volatile organic compounds (VOCs) from aqueous solution using Municipal solid waste biochar (MSW-BC) has been evaluated. Municipal solid waste was pyrolyzed in an onsite pyrolyzer around 450 °C with a holding time of 30 min for the production of biochar (BC). Physiochemical properties of BC were assessed based on X-Ray Fluorescence (XRF) and Fourier transform infra-red (FTIR) analysis. Adsorption capacities for the VOCs (m-xylene and toluene) were examined by batch sorption experiments. Analysis indicated high loading of m-xylene and toluene in landfill leachates from different dump sites. The FTIR analysis corroborates with the Boehm titration data whereas XRF data demonstrated negligible amounts of trace metals in MSW-BC to be a potential sorbent. Adsorption isotherm exhibited properties of both Langmuir and Freundlich which is indicative of a non-ideal monolayer adsorption process taking place. Langmuir adsorption capacities were high as 850 and 550 µg/g for toluene and m-xylene respectively. The conversion of MSW to a value added product provided a feasible means of solid waste management. The produced MSW-BC was an economical adsorbent which demonstrated a strong ability for removing VOCs. Hence, MSW-BC can be used as a landfill cover or a permeable reactive barrier material to treat MSW leachate. Thus, the conversion of MSW to BC becomes an environmentally friendly and economical means of solid waste remediation.

Improvement of carbon usage for phosphorus recovery in EBPR-r and the shift in microbial community.

Enhanced biological phosphorus removal and recovery (EBPR-r) is a biofilm process that makes use of polyphosphate accumulating organisms (PAOs) to remove and recover phosphorus (P) from wastewater. The original process was inefficient, as indicated by the low P-release to carbon (C)-uptake (Prel/Cupt) molar ratio of the biofilm. This study successfully validated a strategy to improve the Prel/Cupt ratio by at least 3-fold. With an unchanged supply of carbon in the recovery stream, an increase in the hydraulic loading in stages I, II and III (7.2, 14.4 and 21.6 L, respectively) resulted in a 43% increase in the Prel/Cupt ratio (0.069, 0.076 and 0.103, respectively). The ratio further increased by 150% (from 0.103 to 0.255) when the duration of the P uptake period was increased from 4 h (stage III) to 10 h (stage IV). Canonical correspondence analysis showed that, correlated to the 3-fold increase in the Prel/Cupt ratio, there was an increase in the abundance of PAOs ("Candidatus Accumulibacter" Clade IIA) and a decrease in the occurrence of glycogen accumulating organisms (GAOs) (family Sinobacteraceae). However, the four stage operation impaired denitrification, resulting in a 5-fold reduction in the Nden/Pupt ratio. The decline in denitrification was consistent with a decrease in the abundance of denitrifiers including denitrifying PAOs (family Comamonadaceae and "Candidatus Accumulibacter" Clade IA). Overall, a strategy to facilitate more efficient use of carbon was validated, enabling a 3-fold carbon saving for P recovery. The new process enabled up to 80% of the wastewater P to be captured in a P-enriched stream (>90 mg/L) with a single uptake/release cycle of recovery.

Assessing the suitability of sediment-type bioelectrochemical systems for organic matter removal from municipal wastewater: a column study.

This study examines the use of bioelectrochemical systems (BES) as an alternative to rock filters for polishing wastewater stabilisation ponds (WSPs) effluent, which often contains soluble chemical oxygen demand (SCOD) and suspended solids mainly as algal biomass. A filter type sediment BES configuration with graphite granules (as the surrogate for rocks in a rock filter) was examined. Three reactor columns were set up to examine three different treatments: (i) open-circuit without current generation; (ii) close-circuit - with current generation; and (iii) control reactor without electrode material. All columns were continuously operated for 170 days with real municipal wastewater at a hydraulic retention time of 5 days. Compared to the control reactor, the two experimental reactors showed significant improvement of SCOD removal (from approximately 25% to 66%) possibly due to retention of biomass on the graphite media. However, substantial amount of SCOD (60%) was removed via non-current generation pathways, and a very low Coulombic efficiency (6%) was recorded due to a poor cathodic oxygen reduction kinetics and a large electrode spacing. Addressing these challenges are imperative to further develop BES technology for WSP effluent treatment.

Simultaneous phosphorus uptake and denitrification by EBPR-r biofilm under aerobic conditions: effect of dissolved oxygen.

A biofilm process, termed enhanced biological phosphorus removal and recovery (EBPR-r), was recently developed as a post-denitrification approach to facilitate phosphorus (P) recovery from wastewater. Although simultaneous P uptake and denitrification was achieved despite substantial intrusion of dissolved oxygen (DO >6 mg/L), to what extent DO affects the process was unclear. Hence, in this study a series of batch experiments was conducted to assess the activity of the biofilm under various DO concentrations. The biofilm was first allowed to store acetate (as internal storage) under anaerobic conditions, and was then subjected to various conditions for P uptake (DO: 0-8 mg/L; nitrate: 10 mg-N/L; phosphate: 8 mg-P/L). The results suggest that even at a saturating DO concentration (8 mg/L), the biofilm could take up P and denitrify efficiently (0.70 mmol e(-)/g total solids*h). However, such aerobic denitrification activity was reduced when the biofilm structure was physically disturbed, suggesting that this phenomenon was a consequence of the presence of oxygen gradient across the biofilm. We conclude that when a biofilm system is used, EBPR-r can be effectively operated as a post-denitrification process, even when oxygen intrusion occurs.

Ammonium-oxidizing bacteria facilitate aerobic degradation of sulfanilic acid in activated sludge.

Sulfanilic acid (SA) is a toxic sulfonated aromatic amine commonly found in anaerobically treated azo dye contaminated effluents. Aerobic acclimatization of SA-degrading mixed microbial culture could lead to co-enrichment of ammonium-oxidizing bacteria (AOB) because of the concomitant release of ammonium from SA oxidation. To what extent the co-enriched AOB would affect SA oxidation at various ammonium concentrations was unclear. Here, a series of batch kinetic experiments were conducted to evaluate the effect of AOB on aerobic SA degradation in an acclimatized activated sludge culture capable of oxidizing SA and ammonium simultaneously. To account for the effect of AOB on SA degradation, allylthiourea was used to inhibit AOB activity in the culture. The results indicated that specific SA degradation rate of the mixed culture was negatively correlated with the initial ammonium concentration (0-93 mM, R²= 0.99). The presence of AOB accelerated SA degradation by reducing the inhibitory effect of ammonium (≥ 10 mM). The Haldane substrate inhibition model was used to correlate substrate concentration (SA and ammonium) and oxygen uptake rate. This study revealed, for the first time, that AOB could facilitate SA degradation at high concentration of ammonium (≥ 10 mM) in an enriched activated sludge culture.

Effective chloramine management without "burn" in biofilm affected nitrifying tanks using a low dose of copper.

This paper highlights the potential to effectively inhibit nitrification and restore chloramine levels using a low copper concentration in a biofilm-affected (surface-to-volume ratio 16 m-1) continuous-flow laboratory-scale chloraminated system. High nitrite and low chloramine containing tanks are always recovered with chlorine "burn" by water utilities. The "burn" is not only costly and operationally complex, but also compromises the water quality, public health, and customer relations. A laboratory system comprising five reactors connected in series was operated. Each reactor simulated conditions typically encountered in full-scale systems. Low amount of copper (0.1-0.2 mg-Cu L-1) was dosed once per day into nitrified reactors. At any given time, only one reactor was dosed with copper. Not only inhibition of nitrification, chloramine decay associated with bulk water, biofilm and sediments also improved. However, the improvement was quicker and more significant when the influent to the reactor contained a high chloramine and a low nitrite concentration. Ammonia oxidising microbes exhibited resilience when exposed to low copper and chloramine concentrations for an extended period. Chloramine decay due to planktonic microbes and chemical reactions in bulk water decreased more rapidly than decay attributed to biofilm and sediments. The concept "biostable residual chlorine" explained how copper and chloramine can inhibit nitrification. Once nitrification was inhibited, the chloramine supplied from upstream effectively continued to suppress downstream nitrification, and this effect lasted more than 50 days even at 22 °C. The findings could be used to develop short-term copper dosing strategies and prevent negative impacts of nitrification and breakpoint chlorination.

Biological phosphorus and nitrogen removal in sequencing batch reactors: effects of cycle length, dissolved oxygen concentration and influent particulate matter.

Removal of phosphorus (P) and nitrogen (N) from municipal wastewaters is required to mitigate eutrophication of receiving water bodies. While most treatment plants achieve good N removal using influent carbon (C), the use of influent C to facilitate enhanced biological phosphorus removal (EBPR) is poorly explored. A number of operational parameters can facilitate optimum use of influent C and this study investigated the effects of cycle length, dissolved oxygen (DO) concentration during aerobic period and influent solids on biological P and N removal in sequencing batch reactors (SRBs) using municipal wastewaters. Increasing cycle length from 3 to 6 h increased P removal efficiency, which was attributed to larger portion of N being removed via nitrite pathway and more biodegradable organic C becoming available for EBPR. Further increasing cycle length from 6 to 8 h decreased P removal efficiencies as the demand for biodegradable organic C for denitrification increased as a result of complete nitrification. Decreasing DO concentration in the aerobic period from 2 to 0.8 mg L(-1) increased P removal efficiency but decreased nitrification rates possibly due to oxygen limitation. Further, sedimented wastewater was proved to be a better influent stream than non-sedimented wastewater possibility due to the detrimental effect of particulate matter on biological nutrient removal.

Ano-cathodophilic biofilm catalyzes both anodic carbon oxidation and cathodic denitrification.

Biocathodic denitrification using bioelectrochemical systems (BES) have shown promise for both wastewater and groundwater treatment. Typically, these systems involve anodic carbon oxidation and cathodic denitrification catalyzed by two electroactive biofilms located separately at an anode and a cathode. However, process efficiencies are often limited by pH drifts in the respective electrode-biofilms: acidification (pH <5.5) in the bioanode and basification (pH >8.5) in the biocathode. Here, we describe for the first time a single electroactive biofilm that acts as a bioanode and a biocathode, alternately catalyzing anodic acetate oxidation (Coulombic efficiency (CE) 85.3%) and cathodic denitrification (CE 87.3%) (-400 mV Ag/AgCl). Our results indicate that the ano-cathodophilic biofilm denitrified autotrophically using the electrode (-200 to -600 mV Ag/AgCl) as a direct electron donor. Further, the alkalinity produced from cathodic denitrification partially (19%) neutralized the acidity of the anodic reaction. Switching the electrode potential to temporarily favor either an anodic or cathodic reaction may represent a unique method for removing carbon and nitrate from contaminated liquors. This study offers new insights into the development of sustainable BES-based nutrient removal processes.

Sequential removal of selenate, nitrate and sulfate and recovery of elemental selenium in a multi-stage bioreactor process with redox potential feedback control.

Bioreduction can facilitate oxyanions removal from wastewater. However, simultaneously removing selenate, nitrate and sulfate and recovering high-purity elemental selenium (Se0) from wastewater by a single system is difficult and may lead to carcinogenic selenium monosulfide (SeS) formation. To solve this issue, a two-stage biological fluidized bed (FBR) process with ethanol dosing based on oxidation-reduction potential (ORP) feedback control was developed in this study. FBR1 performance was first evaluated at various ORP setpoints (between -520 and -360 mV vs. Ag/AgCl) and elevated sulfate concentration. Subsequently, ethanol-fed FBR2 was used to reduce sulfate from FBR1 effluent, followed by an aerated sulfide oxidation reactor (SOR). At - 520 mV≤ ORPs≤ -480 mV, FBR1 removed 100 ±â€¯0.1% nitrate and 99.7 ±â€¯0.3% selenate without sulfate reduction. At ORPs ≥ -440 mV, selenate reduction was incomplete, whereas nitrate removal remained stable. Se0 recovery efficiency from FBR1 effluent was 37.5% with 71% Se purity. FBR2 converted 86% of the remaining sulfate in FBR1 effluent to hydrogen sulfide, but the over-oxidation of dissolved sulfide in SOR decreased the overall sulfate removal efficiency to ~46.3%. Overall, the two-stage FBR process with ORP feedback dosing of ethanol was effective for sequentially removing selenate, nitrate and sulfate and recovering Se0 from wastewater.

Influence of biofilms on iron and manganese deposition in drinking water distribution systems.

Although health risk due to discoloured water is minimal, such water continues to be the source of one of the major complaints received by most water utilities in Australia. Elevated levels of iron (Fe) and/or manganese (Mn) in bulk water are associated with discoloured water incidents. The accumulation of these two elements in distribution systems is believed to be one of the main causes for such elevated levels. An investigation into the contribution of pipe wall biofilms towards Fe and Mn deposition, and discoloured water events is reported in this study. Eight laboratory-scale reactors were operated to test four different conditions in duplicate. Four reactors were exposed to low Fe (0.05 mg l(-1)) and Mn (0.02 mg l(-1)) concentrations and the remaining four were exposed to a higher (0.3 and 0.4 mg l(-1) for Fe and Mn, respectively) concentration. Two of the four reactors which received low and high Fe and Mn concentrations were chlorinated (3.0 mg l(-1) of chlorine). The biological activity (measured in terms of ATP) on the glass rings in these reactors was very low (∼1.5 ng cm(-2) ring). Higher concentrations of Fe and Mn in bulk water and active biofilms resulted in increased deposition of Fe and Mn on the glass rings. Moreover, with an increase in biological activity, an increase in Fe and Mn deposition was observed. The observations in the laboratory-scale experiments were in line with the results of field observations that were carried out using biofilm monitors. The field data additionally demonstrated the effect of seasons, where increased biofilm activities observed on pipe wall biofilms during late summer and early autumn were found to be associated with increased deposition of Fe and Mn. In contrast, during the cooler months, biofilm activities were a magnitude lower and the deposited metal concentrations were also significantly less (ie a drop of 68% for Fe and 86% for Mn). Based on the laboratory-scale investigations, detachment of pipe wall biofilms due to cell death or flow dynamics could release the entrapped Fe and Mn into the bulk water, which could lead to a discoloured water event. Hence, managing biofilm growth on drinking water pipelines should be considered by water utilities to minimize accumulation of Fe and Mn in distribution networks.

An assessment of the persistence of putative pathogenic bacteria in chloraminated water distribution systems.

This study investigated how a chloramine loss and nitrifying conditions influenced putative pathogenic bacterial diversity in bulk water and biofilm of a laboratory- and a full-scale chloraminated water distribution systems. Fifty-four reference databases containing full-length 16S rRNA gene sequences obtained from the National Centre for Biotechnology Information database were prepared to represent fifty-four pathogenic bacterial species listed in the World Health Organisation and Australian Drinking Water Quality Guidelines. When 16S rRNA gene sequences of all samples were screened against the fifty-four reference pathogenic databases, a total of thirty-one putative pathogenic bacteria were detected in both laboratory- and full-scale systems where total chlorine residuals ranged between 0.03 - 2.2 mg/L. Pathogenic bacterial species Mycolicibacterium fortuitum and Pseudomonas aeruginosa were noted in all laboratory (i.e. in bulk water and biofilm) and in bulk water of full-scale samples and Mycolicibacterium fortuitum dominated when chloramine residuals were high. Other different pathogenic bacterial species were observed dominant with decaying chloramine residuals. This study for the first time reports the diverse abundance of putative pathogenic bacteria resilient towards chloramine and highlights that metagenomics surveillance of drinking water can serve as a rapid assessment and an early warning of outbreaks of a large number of putative pathogenic bacteria.

Optimization of nitrate and selenate reduction in an ethanol-fed fluidized bed reactor via redox potential feedback control.

Electron donors are a major cost-factor in biological removal of oxyanions, such as nitrate and selenate from wastewater. In this study, an online ethanol dosing strategy based on feedback control of oxidation-reduction potential (ORP) was designed to optimize the performance of a lab-scale fluidized bed reactor (FBR) in treating selenate and nitrate (5 mM each) containing wastewater. The FBR performance was evaluated at various ORP setpoints ranging between -520 mV and -240 mV (vs. Ag/AgCl). Results suggested that both nitrate and selenate were completely removed at ORPs between -520 mV and -360 mV, with methylseleninic acid, selenocyanate, selenosulfate and ammonia being produced at low ORPs between -520 mV and -480 mV, likely due to overdosing of ethanol. At ORPs between -300 mV and -240 mV, limited ethanol dosing resulted in an apparent decline in selenate removal whereas nitrate removal remained stable. Resuming the ORP to -520 mV successfully restored complete selenate reduction. An optimal ORP of -400 mV was identified for the FBR, whereby selenate and nitrate were nearly completely removed with a minimal ethanol consumption. Overall, controlling ORP via feedback-dosing of the electron donor was an effective strategy to optimize FBR performance for reducing selenate and nitrate in wastewater.

Anammox bacteria in treating ammonium rich wastewater: Recent perspective and appraisal.

The discovery of anammox process has provided eco-friendly and low-cost means of treating ammonia rich wastewater with remarkable efficiency. Furthermore, recent studies have shown that the possibility of operating the anammox process under low temperatures and high organic matter contents broadening the application of the anammox process. However, short doubling time and extensive levels of sensitivity towards nutrients and environmental alterations such as salinity and temperature are the limitations in practical applications of the anammox process. This review article provides the recent yet comprehensive viewpoint on anammox bacteria and the key perspectives in applying them as an efficient strategy for wastewater treatment.

Insights drawn from a full-scale Intermittently Decanted Extended Aeration (IDEA) plant for optimising nitrogen and phosphorus removal from municipal wastewater.

Intermittently Decanted Extended Aeration (IDEA) processes are widely used for wastewater treatment. However, in-depth performance evaluation of a full-scale IDEA plant is rare, making it challenging for water utilities to meet the increasingly stringent discharge requirements with these assets. This study aims to fill this gap through a comprehensive assessment of nitrogen and phosphorus removal in a full-scale IDEA plant in Australia. The plant consists of two identical IDEA tanks operated in-parallel. Upstream to each tank is a bioselector with four interlinked compartments. We conducted an eight-week monitoring program with four intensive cyclic studies to establish detailed nutrient profiles of the two IDEA tanks to assess the performance of nitrogen and alum assisted phosphorus removal. Results showed that the plant enabled good nitrification in the IDEA effluent. However, the denitrification efficiency was low (ca. 50%), and could be improved by decreasing oxygen supply to suppress nitrite oxidation and preserve influent carbon. The addition of alum to the IDEA tank appeared to be ineffective given the low P concentration (<1 mg-P/L) in the tank. The bioselector was identified as a better alum-dosing location, given its higher (~7-fold) phosphate concentration in comparison to the influent. Stopping the dosing of alum only marginally increased the effluent P (0.35 to 0.52 mg-P/L), implying that P removal was predominantly (94%) biologically mediated and achieved via P accumulating microorganisms. Overall, this study offers timely and useful process understanding of the performance of IDEA plants, as well as other similar wastewater treatment configurations.

High-rate microbial selenate reduction in an up-flow anaerobic fluidized bed reactor (FBR).

Wastewater contaminated with high concentrations of selenium oxyanions requires treatment prior to discharge. Biological fluidized bed reactors (FBRs) can be an option for removing selenium oxyanions from wastewater by converting them into elemental selenium, which can be separated from the treated effluent. In this study, a lab-scale FBR was constructed with granular activated carbon as biofilm carrier and inoculated with a consortium of selenate reducing bacteria enriched from environmental samples. The FBR was loaded with an influent containing ethanol (10 mM) and selenate (10 mM) as the microbial electron donor and acceptor, respectively. The performance of the FBR in reducing selenate was evaluated under various hydraulic retention times (HRTs) (120 h, 72 h, 48 h, 24 h, 12 h, 6 h, 3 h, 1 h and 20 min). After process acclimatization, selenate was completely removed with no notable selenite produced when the HRT was stepwise decreased from 120 h to 6 h. However, decreasing the HRT to 3 h resulted in selenite accumulation (0.17 ± 0.023 mM) in the effluent although selenate removal efficiency remained at 99.8 ± 0.20%. At 1 h HRT, the FBR removed 90.8 ± 1.4% of the selenate at a rate of 9.6 ± 0.15 mM h-1, which is the highest selenate reduction rate reported in the literature so far. However, 1 h HRT resulted in notable selenite accumulation (up to 2.4 ± 0.27 mM). Further decreasing the HRT to 20 min resulted in a notable decline in selenate reduction. Selenate reduction recovered from the "shock loading" after the HRT was increased back to 3 h. However, selenite still accumulated until the FBR was operated in batch mode for 6 days. This study affirmed that FBR is a promising treatment option for selenate-rich wastewater, and the process can be efficiently operated at low HRTs.

Sequential hydrotalcite precipitation and biological sulfate reduction for acid mine drainage treatment.

Hydrotalcite precipitation is a promising technology for the on-site treatment of acid mine drainage (AMD). This technology is underpinned by the synthesis of hydrotalcite that can effectively remove various contaminants. However, hydrotalcite precipitation has only limited capacity to facilitate sulfate removal from AMD. Therefore, the feasibility of coupling biological sulfate reduction with the hydrotalcite precipitation to maximize sulfate removal was evaluated in this study. AMD emanating from a gold mine (pH 4.3, sulfate 2000 mg L-1, with various metals including Al, Cd, Co, Cu, Fe, Mn, Ni, Zn) was first treated using the hydrotalcite precipitation. Subsequently, biological treatment of the post-hydrotalcite precipitation effluent was conducted in an ethanol-fed fluidized bed reactor (FBR) at a hydraulic retention time (HRT) of 0.8-1.6 day. The hydrotalcite precipitation readily neutralized the acidity of AMD and removed 10% of sulfate and over 99% of Al, Cd, Co, Cu, Fe, Mn, Ni, Zn. The overall sulfate removal increased to 73% with subsequent FBR treatment. Based on 454 pyrosequencing of 16S rRNA genes, the identified genera of sulfate-reducing bacteria (SRB) included Desulfovibrio, Desulfomicrobium and Desulfococcus. This study showed that sulfate-rich AMD can be effectively treated by integrating hydrotalcite precipitation and a biological sulfate reducing FBR.

A comparative study of methanol as a supplementary carbon source for enhancing denitrification in primary and secondary anoxic zones.

A comparative study on the use of methanol as a supplementary carbon source to enhance denitrification in primary and secondary anoxic zones is reported. Three lab-scale sequencing batch reactors (SBR) were operated to achieve nitrogen and carbon removal from domestic wastewater. Methanol was added to the primary anoxic period of the first SBR, and to the secondary anoxic period of the second SBR. No methanol was added to the third SBR, which served as a control. The extent of improvement on the denitrification performance was found to be dependent on the reactor configuration. Addition to the secondary anoxic period is more effective when very low effluent nitrate levels are to be achieved and hence requires a relatively large amount of methanol. Adding a small amount of methanol to the secondary anoxic period may cause nitrite accumulation, which does not improve overall nitrogen removal. In the latter case, methanol should be added to the primary anoxic period. The addition of methanol can also improve biological phosphorus removal by creating anaerobic conditions and increasing the availability of organic carbon in wastewater for polyphosphate accumulating organisms. This potentially provides a cost-effective approach to phosphorus removal from wastewater with a low carbon content. New fluorescence in situ hybridisation (FISH) probes targeting methanol-utilising denitrifiers were designed using stable isotope probing. Microbial structure analysis of the sludges using the new and existing FISH probes clearly showed that the addition of methanol stimulated the growth of specific methanol-utilizing denitrifiers, which improved the capability of sludge to use methanol and ethanol for denitrification, but reduced its capability to use wastewater COD for denitrification. Unlike acetate, long-term application of methanol has no negative impact on the settling properties of the sludge.