Energy metabolism and muscle activation heterogeneity explain V ̇ O 2 ${\dot{V}}_{{{\rm{O}}}_{\rm{2}}}$ slow component and muscle fatigue of cycling at different intensities.

NEW FINDINGS: What is the central question of this study? What are the physiological mechanisms underlying muscle fatigue and the increase in the O2 cost per unit of work during high-intensity exercise? What is the main finding and its importance? Muscle fatigue happens before, and does not explain, the V ̇ O 2 ${\dot{V}}_{{{\rm{O}}}_{\rm{2}}}$ slow component ( V ̇ O 2 sc ${\dot{V}}_{{{\rm{O}}}_{\rm{2}}{\rm{sc}}}$ ), but they share the same origin. Muscle activation heterogeneity is associated with muscle fatigue and V ̇ O 2 sc ${\dot{V}}_{{{\rm{O}}}_{\rm{2}}{\rm{sc}}}$ . Knowing this may improve training prescriptions for healthy people leading to improved public health outcomes. ABSTRACT: This study aimed to explain the V ̇ O 2 ${\dot{V}}_{{{\rm{O}}}_{\rm{2}}}$ slow component ( V ̇ O 2 sc ${\dot{V}}_{{{\rm{O}}}_{\rm{2}}{\rm{sc}}}$ ) and muscle fatigue during cycling at different intensities. The muscle fatigue of 16 participants was determined through maximal isokinetic effort lasting 3 s during constant work rate bouts of moderate (MOD), heavy (HVY) and very heavy intensity (VHI) exercise. Breath-by-breath V ̇ O 2 ${\dot{V}}_{{{\rm{O}}}_{\rm{2}}}$ , near-infrared spectroscopy signals and EMG activity were analysed (thigh muscles). V ̇ O 2 sc ${\dot{V}}_{{{\rm{O}}}_{\rm{2}}{\rm{sc}}}$ was higher during VHI exercise (∼70% vs. ∼28% of V ̇ O 2 ${\dot{V}}_{{{\rm{O}}}_{\rm{2}}}$ reserve in HVY). The deoxygenated haemoglobin final value during VHI exercise was higher than during HVY and MOD exercise (∼90% of HHb physiological normalization, vs. ∼82% HVY and ∼45% MOD). The muscle fatigue was greater after VHI exercise (∼22% vs. HVY ∼5%). There was no muscle fatigue after MOD exercise. The greatest magnitude of muscle fatigue occurred within 2 min (VHI ∼17%; HVY ∼9%), after which it stabilized. No significant relationship between V ̇ O 2 sc ${\dot{V}}_{{{\rm{O}}}_{\rm{2}}{\rm{sc}}}$ and muscle force production was observed. The τ of muscle V ̇ O 2 ${\dot{V}}_{{{\rm{O}}}_{\rm{2}}}$ was significantly related (R2  = 0.47) with torque decrease for VHI. Type I and II muscle fibre recruitment mainly in the rectus femoris moderately explained the muscle fatigue (R2  = 0.30 and 0.31, respectively) and the V ̇ O 2 sc ${\dot{V}}_{{{\rm{O}}}_{\rm{2}}{\rm{sc}}}$ (R2  = 0.39 and 0.27, respectively). The V ̇ O 2 sc ${\dot{V}}_{{{\rm{O}}}_{\rm{2}}{\rm{sc}}}$ is also partially explained by blood lactate accumulation (R2  = 0.42). In conclusion muscle fatigue and O2 cost seem to share the same physiological cause linked with a decrease in the muscle V ̇ O 2 ${\dot{V}}_{{{\rm{O}}}_{\rm{2}}}$ and a change in lactate accumulation. Muscle fatigue and V ̇ O 2 sc ${\dot{V}}_{{{\rm{O}}}_{\rm{2}}{\rm{sc}}}$ are associated with muscle activation heterogeneity and metabolism of different muscles activated during cycling.

Similar Time Near VO2max Regardless of Work Rate Manipulation in Cycling Interval Training.

The current study aimed to compare time spent above 90% V̇O2max (tV̇O2max) during 3 work-matched interval training protocols comprising 8×60-second exercise efforts with decreasing, increasing, or constant work rate distribution within each exercise interval. Ten healthy male subjects (age: 27.6±5.0 years; V̇O2max: 3.82±0.52 L•min-1) performed an incremental test to determine V̇O2max and peak power output (P max). During visits 2, 3, and 4, three work-matched interval training sessions comprising 8×60 s efforts: 60 s active recovery with the power output held constant (100%Pmax; ITCON), decreasing (from 110 to 90%Pmax; ITDEC), or increasing (from 90 to 110%Pmax; ITINC) linearly throughout each work interval. Time sustained above 90% of V̇O2max (tV̇O2max) or HRmax (tHRmax), blood lactate concentrations (BLC) and rating of perceived exertion (RPE) were measured. The tV̇O2max (ITCON: 274±132; ITDEC: 313±102; ITINC: 310±113 s, P=0.37), tHRmax (ITCON: 396±180; ITDEC: 441±207; ITINC: 390±212 s, P=0.47), BLC (P=0.73), and final RPE (P=0.75) were similar among protocols. In conclusion, work-matched interval training induced similar time near V̇O2max and associated physiological responses regardless of work rate manipulation.

A novel treadmill protocol for uphill running assessment: the incline incremental running test (IIRT).

This study aimed to compare the maximal and submaximal aerobic parameters between two incremental running tests, one being horizontal and the other an incline-based test, namely the incline incremental running test (IIRT). Twenty endurance-trained trail runners completed two incremental treadmill tests, until exhaustion. The first test was performed using an incline of 1%, with speed increments. Then, the IIRT was performed with the speed set at 50% of the peak speed obtained during the previous test, and the incline was incremented. Cardiorespiratory measurements and blood lactate concentration ([La]) were assessed. The mean peak workload from the horizontal test was 17.6 ± 1.4 km.h-1 and peak workload from IIRT was 17.3 ± 1.3% of incline. The VO2peak and [La]peak were not significantly different between the protocols. However, the HRpeak was significantly lower at IIRT. In conclusion, most of the maximal and submaximal aerobic indices showed no differences between the incremental tests analysed. The exceptions were the HRpeak and HR at the lactate turnpoints, that were lower, and the peak O2 pulse that was greater for the IIRT. Taken together, these data support the validity of the IIRT as a specific test for the physiological assessment of runners involved with uphill performances.

Game Running Performance and Fitness in Women's Futsal.

The study aimed to verify possible associations between game-play running performance and outcomes derived from fitness (running) tests in female futsal players. Sixteen women professional elite futsal players from a 1st division league team (19.2±2 years-old, 4.3±2.1 years of experience) participated. Firstly, a graded incremental treadmill test was adopted to determine maximal oxygen uptake (VO2max). Following 72 h of laboratory protocol, players were asked to perform a repeated-sprint test on a court (8×40 m with two 180° change-of-directions). Twenty-four hours after, players participated in a one-off friendly game (two 20-min half-times). A computerized automatic image recognition software (DVIDEOW; 30 Hz) allowed to determine game running performance variables. Fatigue index and best time in the court test and VO2max and its attached speed derived from laboratory-based test showed significant moderate-to-moderately high correlations (r=- 0.59-0.76; p<0.05) with some game running performance outputs, notably related to high-intensity running. In conclusion, the present study provided initial evidence on associations between two fitness tests and one-off game running performance in female futsal. Information derived from the work potentially help conditioning professionals working with female futsal athletes gain awareness about some properties of common testing tools.

Rate of utilization of a given fraction of W' (the curvature constant of the power-duration relationship) does not affect fatigue during severe-intensity exercise.

NEW FINDINGS: What is the central question of this study? Does the rate of utilization of W' (the curvature constant of the power-duration relationship) affect fatigue during severe-intensity exercise? What is the main finding and its importance? The magnitude of fatigue after two severe-intensity exercises designed to deplete the same fraction of W' (70%) at two different rates of utilization (fast versus slow) was similar after both exercises. Moreover, the magnitude of fatigue was related to critical power (CP), supporting the contention that CP is a key determinant in fatigue development during high-intensity exercise. Thus, the CP model is a suitable approach to investigate fatigue mechanisms during high-intensity exercise. The depletion of W' (the curvature constant of the power-duration relationship) seems to contribute to fatigue during severe-intensity exercise. Therefore, the aim of this study was to determine the effect of a fast versus a slow rate of utilization of W' on the occurrence of fatigue within the severe-intensity domain. Fifteen healthy male subjects performed tests to determine the critical power, W' and peak torque in the control condition (TCON ) and immediately after two fatiguing work rates (THREE and TEN) set to deplete 70% W' in either 3 (TTHREE ) or 10 min (TTEN ). The TTHREE and TTEN were significantly reduced (F = 19.68, P = 0.01) in comparison to TCON . However, the magnitude of reduction in peak torque (TTHREE  = -19.8 ± 10.1% versus TTEN  = -16.8 ± 13.3%) was the same in the two fatiguing exercises (t = -0.76, P = 0.46). There was a significant inverse relationship between the critical power and the reduction in peak torque during both THREE (r = -0.49, P = 0.03) and TEN (r = -0.62, P = 0.02). In contrast, the W' was not significantly correlated with the reduction in peak torque during both THREE (r = -0.14, P = 0.33) and TEN (r = -0.30, P = 0.10). Thus, fatigue following severe-intensity exercises performed at different rates of utilization of W' was similar when the same work was done above the critical power (i.e. same amount of W' used).

The peak velocity derived from the Carminatti Test is related to physical match performance in young soccer players.

The aim of this study was to examine the relationship between the peak velocity derived from the Carminatti Test (T-CAR) (PVT-CAR) and physical match performance in young soccer players. Thirty-three youth soccer players were recruited from 2 non-professional clubs. Friendly matches and small-sided game were performed. Physical match demands were assessed using Global Positioning System (GPS) technology. On a separate occasion, the players were submitted to the T-CAR. Players were categorised into 3 groups based on their T-CAR performance: Low (PVT-CAR ≤ P33), Intermediate (P33 > PVT-CAR < P66) and High (PVT-CAR ≥ P66). The PVT-CAR (15.5 ± 0.7 km·h-1) was significantly related to high-intensity activities (HIA; r = 0.78, P < 0.001), high-intensity running (HIR; r = 0.66, P < 0.001), sprinting (r = 0.62, P < 0.001) and total distance (TD) covered (r = 0.47, P < 0.01) during friendly matches. The PVT-CAR was strongly correlated with the amount of HIA (r = 0.81, P < 0.001), HIR (r = 0.85, P < 0.001) and TD covered (r = 0.81, P < 0.001) during small-sided game. No significant correlation was observed between the PVT-CAR and distance of sprinting (r = 0.49, P = 0.067) during small-side game. Furthermore, players in the High group covered significantly more TD (10%) and did more HIA (42%), sprinting (31%) and HIR (25%) during friendly matches compared to the players classified as having Low performance on the T-CAR. These differences still remained after adjusting for chronological age (CA), maturity and body size. In conclusion, the current study gives empirical support to the ecological and construct validity of this novel field test (T-CAR) as an indicator of match-related physical performance in young soccer players during pubertal years.

The effect of prior exercise intensity on oxygen uptake kinetics during high-intensity running exercise in trained subjects.

PURPOSE: The aim of this study was to compare the effects of two different kinds of prior exercise protocols [continuous exercise (CE) versus intermittent repeated sprint (IRS)] on oxygen uptake (VO2) kinetics parameters during high-intensity running. METHODS: Thirteen male amateur futsal players (age 22.8 ± 6.1 years; mass 76.0 ± 10.2 kg; height 178.7 ± 6.6 cm; VO2max 58.1 ± 4.5 mL kg(-1) min(-1)) performed a maximal incremental running test for the determination of the gas exchange threshold (GET) and maximal VO2 (VO2max). On two different days, the subjects completed a 6-min bout of high-intensity running (50 % ∆) on a treadmill that was 6-min after (1) an identical bout of high-intensity exercise (from control to CE), and (2) a protocol of IRS (6 × 40 m). RESULT: We found significant differences between CE and IRS for the blood lactate concentration ([La]; 6.1 versus 10.7 mmol L(-1), respectively), VO2 baseline (0.74 versus 0.93 L min(-1), respectively) and the heart rate (HR; 102 versus 124 bpm, respectively) before the onset of high-intensity exercise. However, both prior CE and prior IRS significantly increased the absolute primary VO2 amplitude (3.77 and 3.79 L min(-1), respectively, versus control 3.54 L min(-1)), reduced the amplitude of the VO2 slow component (0.26 and 0.21 L min(-1), respectively, versus control 0.50 L min(-1)), and decreased the mean response time (MRT; 28.9 and 28.0 s, respectively, versus control 36.9 s) during subsequent bouts. CONCLUSION: This study showed that different protocols and intensities of prior exercise trigger similar effects on VO2 kinetics during high-intensity running.

The effect of two generic aerobic interval training methods on laboratory and field test performance in soccer players.

The purpose of this study was to compare the effects of 2 generic aerobic training models, based on peak running velocity in Carminatti's test (PVT-CAR) in U-20 elite soccer players. Seventeen soccer players (age: 17.9 ± 1.0 years; 178.6 ± 5.0 cm; 73.6 ± 6.6 kg; 11.1 ± 1.3%) from a team competing in a national junior league took part in the study. The athletes performed a series of pre- and posttraining tests (incremental test on a treadmill to determine the maximal oxygen uptake [(Equation is included in full-text article.)], velocity at maximal oxygen uptake [(Equation is included in full-text article.)], the lactate threshold [LT], and T-CAR). The interval training models applied were with 180° direction change (T12:12; n = 9) and without direction change (T6:6; n = 8). No significant interaction (time vs. group) was observed for the majority of variables analyzed (p > 0.05), although significant main effects in time were evident regarding peak treadmill velocity (PVTREAD) (F = 56.3, p < 0.0001), (Equation is included in full-text article.)(F = 35.8, p < 0.0001), LT (F = 57.7, p < 0.0001), and PVT-CAR (F = 52.9, p < 0.0001). Moreover, there was no significant change in (Equation is included in full-text article.)between pre and posttraining period (F = 4.26, p = 0.056) in both training groups. Thus, it can be concluded that the prescribed training with and without direction change in the intensity of the PVT-CAR increases the PVTREAD, the (Equation is included in full-text article.), the LT, and the PVT-CAR similarly.

Increased platelet oxidative metabolism, blood oxidative stress and neopterin levels after ultra-endurance exercise.

The purpose of the present investigation was to identify muscle damage, inflammatory response and oxidative stress blood markers in athletes undertaking the ultra-endurance MultiSport Brazil race. Eleven well-trained male athletes (34.3 ± 3.1 years, 74.0 ± 7.6 kg; 172.2 ± 5.1 cm) participated in the study and performed the race, which consisted of about 90 km of alternating off-road running, mountain biking and kayaking. Twelve hours before and up to 15 minutes after the race a 10 mL blood sample was drawn in order to measure the following parameters: lactate dehydrogenase and creatine kinase activities, lipid peroxidation, catalase activity, protein carbonylation, respiratory chain complexes I, II and IV activities, oxygen consumption and neopterin concentrations. After the race, plasma lactate dehydrogenase and creatine kinase activities were significantly increased. Erythrocyte TBA-RS levels and plasma protein carbonylation were markedly augmented in post-race samples. Additionally, mitochondrial complex II activity and oxygen consumption in post-race platelet-rich plasma were also increased. These altered biochemical parameters were accompanied by increased plasma neopterin levels. The ultra-endurance event provoked systemic inflammation (increased neopterin) accompanied by marked oxidative stress, likely by increasing oxidative metabolism (increased oxidative mitochondrial function). This might be advantageous during prolonged exercise, mainly for efficient substrate oxidation at the mitochondrial level, even when tissue damage is induced.

Match-Play Running Performance in Professional Male Soccer Players: The Role of Anaerobic Speed Reserve.

Purpose: This study aimed to compare match-play running performance between players with a high (HASR) and low (LASR) anaerobic speed reserve (ASR) profile in professional soccer players. Method: Thirty-six male players from the same club (age: 23.3 ± 5.4 years) took part in this study. Match data were collected from 43 home matches of the first and second division of the Brazilian National Championship over two consecutive seasons. Players had their maximal sprinting (MSS) and aerobic speeds (MAS) determined during field test-based assessments. ASR was defined as MSS minus MAS. Total distance (TD), sprinting distance, number of accelerations and decelerations, absolute peak (PMS(abs)) and mean match sprint speed (MMSS(abs)) and its relative values expressed as a percentage of MSS (PMS(rel) and MMSS(rel)), distance covered > 100% MAS(abs) and > 50% MSS(abs) were compared between the groups and halves. Results: HASR presented greater sprint demand (d > 0.80), PMS(abs) (d = 1.57), and MMSS(abs) (d = 0.99) than the LASR group. The opposite was observed for PMS(rel) and MMSS(rel). The TD and amount of high-intensity accelerations and decelerations were moderately (0.77MAS(abs) and > 50% MSS(abs) decreased between halves (d = 0.72 and 0.77), while the HASR did not (d < 0.10). Conclusion: Our findings indicated that players with larger ASR had greater sprint demand, with a lower relative running intensity, and covered longer distances above MAS. The amplitude of ASR also affected the player's running ability between halves.

The Effects of a Supramaximal Intermittent Training Program on Aerobic and Anaerobic Running Measures in Junior Male Soccer Players.

This study investigated the effectiveness of supplementing regular preseason soccer training with a supramaximal intermittent shuttle-run training (ISRT) model prescribed from Carminatti's Test peak speed (PST-CAR) in aerobic performance-related indices and sprinting speed in male junior soccer players. Twenty-three national-level soccer players (mean ± SD; age 18.07 ± 0.9 y, body height 1.76 ± 0.65 m, body mass 71.9 ± 8.7 kg) were assigned to either an experimental group (EG; n = 13) performing ISRT + soccer training or a control group (CG; n = 10) that followed regular preseason soccer training alone. The following tests were applied before and after the eight-week training intervention: (i) incremental treadmill tests (VO2max and lactate minimum speed - LMS); (ii) linear 30-m sprint test and Carminatti's Test (PST-CAR). Results indicated larger gains for the EG in LMS (Δ = 9.53% vs. 2.82%) and PST-CAR (Δ = 5.50% vs. 2.10%) than in the CG. Furthermore, changes in VO2max produced higher effect size (d) values for the EG (Δ = 6.67%; d = 0.59) than the CG (Δ = 1.88%; d = 0.18). Both groups improved (p = 0.002) their flying 20-m sprint speed (EG: Δ = 1.01%; CG: Δ = 1.56%). However, small decreases were observed for 10-m sprint speed in the CG (Δ = -2.19%; d = -0.44), while only trivial changes were noticed for the EG (Δ = -0.50%; d = -0.16). Our data support that additional supramaximal ISRT is an effective training stimulus to enhance aerobic performance-related indices and promote small improvements in maximal running speed without impairing the soccer players' acceleration capacity. This study also shows that PST-CAR can be useful for individualizing running intensity in supramaximal ISRT modes.

Reliability and Validity of Cycling Sprint Performance at Isolinear Mode Without Torque Factor: A Preliminary Study in Well-Trained Male Cyclists.

Purpose: This study aimed to compare the performance-derived parameters utilizing isolinear (ISOLIN) and isovelocity (ISOVEL) sprint cycling modes. Method: For that, 20 male trained cyclists performed 2 sprints of 7 s on an electromagnetically braked cycle ergometer in ISOLIN and six sprints in ISOVEL mode with cadences between 90 and 180 rpm, each separated by 3-min. A linear function modeled the sprints within each mode to extrapolate maximal cadence (CMAX) and torque (TMAX), and a quadratic function was used to extrapolate the apex defined as optimal cadence power (OPTCAD) and peak power output (PMAX). Fifteen subjects performed another 4 sprints at ISOLIN mode on different days to verify the reliability. Results: The measures from the power-cadence relationship were not different between the ISOLIN and ISOVEL modes. Although significant differences were detected in the T-C relationship, TMAX was greater at ISOLIN than ISOVEL (p = .006). On the other hand, CMAX was higher at ISOVEL than ISOLIN (p < .001). The correlation between parameters was large to very large (r = 0.51 to 0.89). However, high limits of agreement were verified. The ISOLIN presented consistency during the trials, and the random errors were acceptable (CV = 5.3% to 11.5%). Conclusion: Using the power-cadence relationship, PMAX and OPTCAD could be detected similarly between the two sprint modes (ISOLIN and ISOVEL). Thus, the findings demonstrated that a single ISOLIN sprint test could be a suitable tool for quantifying the time course of muscle fatigue during and after cycling exercises in well-trained male cyclists.

Match activity profile and heart rate responses of top-level soccer referees during Brazilian national first and second division and regional championships.

This study aimed to examine the differences in physical match activities and heart rate (HR) responses experienced by Brazilian field referees (FRs) and assistant referees (ARs) during official matches from Serie A, Serie B and Regional championships. The sample was composed of 51 FRs and 87 ARs. Match activities derived from Global Position System and HR response were collected during official matches. FRs and ARs covered higher distances at high-intensity running (HIR; d = 1.17 and 2.53, p < 0.001), sprinting (d = 0.82 and 0.48, p < 0.005) and high-intensity activities (HIA; d = 1.07 and 1.97, p < 0.001) in Serie A and Serie B than in Regional. Changes in the distances covered at HIR (0.80 < d < 3.25, p < 0.005), sprinting (d = 0.42, p = 0.050) and HIA (0.93 < d < 3.01, p < 0.005) between half-matches were observed during Serie A and Serie B. The average %HRmax was progressively increased in both half-matches (0.85 < d < 3.54, p < 0.001) during all championships. For FRs, Serie A and Regional elicited the highest average %HRmax (0.80 3C d < 1.37, p < 0.030). For ARs, Serie A and Serie B elicited the highest average %HRmax (2.14 < d< 3.87, p < 0.001). In conclusion, the physical match activities and average %HRmax of Brazilian FRs and ARs differ across championship levels. FRs and ARs acting in Regional championships are likely to need specific preparation to support the higher physical demand observed at higher competition levels.

Effects of physical training on functional, clinical, morphological, behavioural and psychosocial outcomes in post-COVID-19 infection: COVID-19 and REhabilitation study (CORE-study)-a study protocol for a randomised controlled clinical trial.

BACKGROUND: The COVID-19 pandemic remains ongoing, with a significant number of survivors who have experienced moderate to severe clinical conditions and who have suffered losses of great magnitude, especially in functional capacity, triggering limitations to daily autonomy and quality of life. Among the possibilities of intervention for disease rehabilitation, physical exercise training stands out, which can benefit several health outcomes and favours the adoption of healthier behaviours. Therefore, the aim of the study will be to analyse the effects of physical training on the functional, clinical, morphological, behavioural and psychosocial status in adults and the elderly following COVID-19 infection. METHODS: A randomised controlled clinical trial is to be conducted in parallel, with the experimental group undergoing an intervention involving a multicomponent physical rehabilitation programme, carried out at the Sports Center in partnership with the Academic Hospital of the Federal University of Santa Catarina, in Florianópolis, Brazil. Participants will be adults and the elderly, of both sexes, in a post-COVID-19-infection state, who were hospitalised during the infection. The intervention will have a total duration of 24 weeks and will include a multicomponent physical training programme, which will have gradual progression in frequency, duration and intensity over time. Regarding the outcomes, before, at the 12th and after 24 weeks of intervention, functional (primary outcome = functional index of aerobic capacity), clinical, morphological, behavioural and psychosocial outcomes will be assessed. DISCUSSION: This study will contribute to a greater understanding of the safety, adherence and benefits of physical training in the rehabilitation of post-COVID-19 patients. The results of this study will be disseminated through presentations at congresses, workshops, peer-reviewed publications and local and international conferences, especially with a view to proposing a post-COVID-19 rehabilitation care protocol. TRIAL REGISTRATION: ReBEC, RBR-10y6jhrs . Registered on 22 February 2022. 2015.

Heart rate variability kinetics during different intensity domains of cycling exercise in healthy subjects.

The purpose of this study was to verify the heart rate variability (HRV) and heart rate (HR) kinetics during the fundamental phase in different intensity domains of cycling exercise. Fourteen males performed five exercise sessions: (1) maximal incremental cycling test; (2) two rest-to-exercise transitions for each intensity domain, that is, heavy (Δ30) and severe (Δ60) domains. HRV markers (SD1 and SD2) and HR kinetics in the fundamental phase were analyzed by first-order exponential fitting. There were no significant differences in amplitude values between SD1Δ30 (8.98 ± 3.52 ms) and SD1Δ60 (9.44 ± 3.24 ms) and SD2Δ30 (24.93 ± 9.16 ms) and SD2Δ60 (25.98 ± 7.29 ms). Significant difference was observed between HRΔ30 (52 ± 7 bpm) and HRΔ60 (63 ± 8 bpm). The time constant (τ) values were significantly different between SD1Δ30 (17.61 ± 6.26 s) and SD1Δ60 (13.86 ± 5.90 s), but not between SD2Δ30 (20.06 ± 3.73 s) and SD2Δ60 (19.47 ± 6.03 s) or HRΔ30 (56.75 ± 18.22 s) and HRΔ60 (58.49 ± 15.61 s). However, the τ values for HRΔ30 were higher and significantly different in relation to SD1Δ30 and SD2Δ30, as well as for HRΔ60 in relation to SD1Δ60 and SD2Δ60. The kinetics of the autonomic variable (SD1 marker) was accelerated by the increased intensity. Moreover, significant differences were found for the τ values, with faster HRV markers than HR, in both intensities of Δ30 and Δ60, which suggests that these variables indicate distinct and specific cardiac autonomic response times during different intensity domains in cycling.HIGHLIGHTSThe application of HRV to optimize exercise prescription at different effort intensities is extremely important to obtain assertive and effective results.Analysis of the kinetic responses of HRV is a useful tool for the evaluation of exercise performance and health status.A faster kinetics was found for HRV markers in comparison to HR, for both intensities analysed, which suggests that these variables indicate distinct and specific cardiac autonomic response times during different intensity domains in cycling.

Validity of Carminatti's test to determine physiological indices of aerobic power and capacity in soccer and futsal players.

The aim of this study was to verify the validity of a new progressive distance and fixed time test (Carminatti's test [TCAR]) in estimating the main physiological indices of aerobic fitness in team-sport players. Thirty professional national level team-sport players (n = 12 futsal players and 18 soccer players) volunteered to participate in this study. The subjects performed the TCAR and a laboratory incremental treadmill test (ITT). The TCAR required subjects to complete repeated sets of 5 × 12-second shuttle-running bouts at progressive speed until volitional exhaustion. Each 12-second bout and series were separated by a 6- and 90-second recovery periods, respectively. The initial distance was set at 15 m and was progressively increased by 1 m each set. The ITT commenced at a velocity of 9.0 km·h(-1) and was increased by 1.2 km·h(-1) each 3 minutes until volitional exhaustion. Peak TCAR running velocity resulted not significantly (p > 0.05) different from speed at VO2max (vVO2max) during ITT. Peak TCAR running velocity was significantly correlated (p < 0.01) with vVO2max (r = 0.55) and VO2max (r = 0.51). No significant differences were found (p > 0.05) among the mean values of velocity and heart rate at the anaerobic threshold, estimated in the TCAR test and measured in the ITT. In light of this study results, the TCAR can be considered as a viable field test to estimate aerobic power and capacity in team-sports players. The limited devices and space required by TCAR warrant consideration for those strength and conditioning professionals who deal with team sports.

High-Intensity Intermittent Exercise Performed on the Sand Induces Higher Internal Load Demands in Soccer Players.

This study aimed to examine the acute physiological effect of shuttle-run-based high-intensity intermittent exercise (HIIE) performed at the same relative speed (i. e., 100% PST-CAR) on sand (SAND) and grass (GRASS) in male junior soccer players. Seven Under-23 Brazilian national league ("Série A") soccer players completed four testing sessions in either SAND or GRASS surface condition. The first two testing sessions consisted of performing a maximal progressive shuttle-run field protocol until volitional exhaustion (Carminatti's test, T-CAR), whereas the third and fourth sessions comprised a HIIE session on each ground surface. The HIIE session consisted of three 5-min bouts [12 s shuttle-run (with a direction change every 6 s)/12 s of passive rest] performed at 100% of T-CAR peak speed (PST-CAR) with 3 min of passive recovery between sets. Measurements of oxygen uptake (VO2), heart rate (HR), blood lactate concentration ([La]), and rating of perceived exertion (RPE) were performed during all conditions. The SAND condition elicited significantly higher %VO2peak (94.58 ± 2.73 vs. 87.45 ± 3.31%, p < 0.001, d = 2.35), %HRpeak (93.89 ± 2.63 vs. 90.31 ± 2.87%, p < 0.001, d = 1.30), RPE (8.00 ± 0.91 vs. 4.95 ± 1.23 a.u., p < 0.001, d = 2.82), and [La] (10.76 ± 2.37 vs. 5.48 ± 1.13 mmol/L, p < 0.010, d = 2.84). This study showed that higher internal workloads are experienced by the players during a single HIIE session performed on a softer surface as SAND, even when the exercise intensity was individualized based on 100%PST-CAR.

Modeling the depletion and reconstitution of W': Effects of prior exercise on cycling tolerance.

Thirteen healthy male subjects (age 28 ±â€¯7 years) performed tests for critical power and W' determination and two square-wave high-intensity exercises until exhaustion either with prior very-heavy intensity cycling (EXP) or without (CON). Prior exercise bout induced a depletion of 60 % of W'. After 10 min of recovery, W' reconstitution was not fully achieved (∼ 92 %). Time to exhaustion and Δ blood lactate concentration were significantly lower in EXP compared to CON (595 ±â€¯118 s vs. 683 ±â€¯148 s; 3.5 ±â€¯1.2 mmol.L-1 vs. 8.8 ±â€¯2.3 mmol.L-1; p < 0.05, respectively). Oxygen uptake (VO2) and heart rate were significantly higher in EXP, during the first 150 s of exercise (p < 0.05). The carbon dioxide production kinetics was significantly slower in EXP (mean response time = 87.8 ±â€¯17.8 s vs. 73.7 ±â€¯16.6 s in CON; p < 0.05). Thus, prior exercise impairs high-intensity cycling performance which can partly be explained by physiological disturbances linked to W' depletion.

Impaired dopamine metabolism is linked to fatigability in mice and fatigue in Parkinson's disease patients.

Fatigue is a common symptom of Parkinson's disease that compromises significantly the patients' quality of life. Despite that, fatigue has been under-recognized as symptom, its pathophysiology remains poorly understood, and there is no adequate treatment so far. Parkinson's disease is characterized by the progressive loss of midbrain dopaminergic neurons, eliciting the classical motor symptoms including slowing of movements, muscular rigidity and resting tremor. The dopamine synthesis is mediated by the rate-limiting enzyme tyrosine hydroxylase, which requires tetrahydrobiopterin as a mandatory cofactor. Here, we showed that reserpine administration (1 mg/kg, two intraperitoneal injections with an interval of 48 h) in adult Swiss male mice (8-10 weeks; 35-45 g) provoked striatal depletion of dopamine and tetrahydrobiopterin, and intolerance to exercise. The poor exercise performance of reserpinized mice was not influenced by emotional or anhedonic factors, mechanical nociceptive thresholds, electrocardiogram pattern alterations or muscle-impaired bioenergetics. The administration of levodopa (100 mg/kg; i.p.) plus benserazide (50 mg/kg; i.p.) rescued reserpine-induced fatigability-like symptoms and restored striatal dopamine and tetrahydrobiopterin levels. Remarkably, it was observed, for the first time, that impaired blood dopamine metabolism inversely and idependently correlated with fatigue scores in eighteen idiopathic Parkinson's disease patients (male n = 13; female n = 5; age 61.3 ± 9.59 years). Altogether, this study provides new experimental and clinical evidence that fatigue symptoms might be caused by the impaired striatal dopaminergic neurotransmission, pointing to a central origin of fatigue in Parkinson's disease.

Phase Angle Is Related to 10 m and 30 m Sprint Time and Repeated-Sprint Ability in Young Male Soccer Players.

OBJECTIVE: To examine the association between phase angle (PhA) and bioelectrical impedance vector analysis (BIVA) and components of physical performance in male youth soccer players. DESIGN: Cross-sectional. METHODS: Sixty-two players from two professional soccer academies were recruited. Electrical bioimpedance was used to obtain the PhA and BIVA. Body fat (BF) and lean soft tissue mass (LSTM) were measured by dual-energy X-ray absorptiometry. All players completed physical tests including the standing long jump (SLJ), Carminatti's test (peak speed at the end of the test, PST-CAR), 10 m and 30 m straight-line sprints, and repeated-sprint ability (RSA) test (RSAbest and RSAmean times). RESULTS: Adjusting for chronological age, BF, and LSTM, multiple regression analysis outputs showed that PhA remained inversely related to RSAmean (ß = -0.362; p < 0.001), RSAbest (ß = -0.239; p = 0.020), 10 m (ß = -0.379; p = 0.012), and 30 m (ß = -0.438; p < 0.001) sprint times, while the association with PST-CAR and SLJ performance were statistically non-significant. In addition, BIVA showed that differences in confidence ellipses were found between athletes in the reference population and the study sample (p < 0.05). The tolerance ellipses indicated that the athletes in the present study had more total body water (TCW) and lower proportions of intracellular water (ICW) to extracellular water (ECW). The reference population had more TCW and ICW/ECW. CONCLUSIONS: Our results suggest that young soccer players with higher PhA values, indicating better cell integrity and functionality, have better performance in typical anaerobic running activities, such as sprinting speed and RSA performance, adjusted to age and body composition characteristics.