Pyridinium-Based Strategy for a Bioorthogonal Conjugation-Assisted Purification Method for Profiling Cell Surface Proteome.

Cell surface proteins (CSPs) are valuable targets for therapeutic agents, but achieving highly selective CSP enrichment in cellular physiology remains a technical challenge. To address this challenge, we propose a newly developed sulfo-pyridinium ester (SPE) cross-linking probe, followed by two-step imaging and enrichment. The SPE probe showed higher efficiency in labeling proteins than similar NHS esters at the level of cell lysates and demonstrated specificity for Lys in competitive experiments. More importantly, this probe could selectively label the cell membranes in cell imaging with only negligible labeling of the intracellular compartment. Moreover, we successfully performed this strategy on MCF-7 live cells to label 425 unique CSPs from 1162 labeled proteins. Finally, we employed our probe to label the CSPs of insulin-cultured MCF-7, revealing several cell surface targets of key functional biomarkers and insulin-associated pathogenesis. The above results demonstrate that the SPE method provides a promising tool for the selective labeling of cell surface proteins and monitoring transient cell surface events.

Development of Lysine Crotonyl-Mimic Probe to Covalently Identify H3K27Cr Interacting Proteins.

Histone lysine crotonylation (Kcr) is one newly discovered acylation modification and regulates numerous pathophysiological processes. The binding affinity between Kcr and its interacting proteins is generally weak, which makes it difficult to effectively identify Kcr-interacting partners. Changing the amide of crotonyl to an ester increased reactivity with proximal cysteines and retained specificity for Kcr antibody. The probe "H3g27Cr" was designed by incorporating the ester functionality into a H3K27 peptide. Using this probe, multiple Kcr-interacting partners including STAT3 were successfully identified, and this has not been reported previously. Further experiments suggested that STAT3 possibly could form complexes with Histone deacetylase HDACs to downregulate the acetylation and crotonylation of Histone H3K27. Our unique design provided intriguing tools to further explore Kcr-interacting proteins and elucidate their working mechanisms.

Flavin catalyzed desulfurization of peptides and proteins in aqueous media.

A biomimetic method has been established for the chemo-selective desulfurization of cysteinyl peptides and proteins in aqueous media. The derivatives of biocatalytic cofactors, flavins, were found to be efficient photosensitizers in a thiyl-radical-mediated desulfurization of Cys. The reaction was conducted in an ultrafast manner with both polypeptides and proteins.

Sulfamethoxazole degradation by Pseudomonas silesiensis F6a isolated from bioelectrochemical technology-integrated constructed wetlands.

The antibiotic-degrading ability and mechanism of the bacteria in the novel and ecological bioelectrochemical technology-integrated constructed wetlands (BICW) remain unknown. In this study, the sulfamethoxazole (SMX) degrading strain Pseudomonas silesiensis F6a (F6a), which had high degradation efficiency, was firstly isolated from a substrate sample in BICW. The SMX degradation process of F6a follows pseudo first order kinetics. Four metabolic pathways and twelve degradation products were identified. Based on genomics and proteomics analysis, six key SMX-degrading genes, Gene4641 deoC, Gene0552 narI, Gene0546 luxS, Gene1753 nuoH, Gene0655 and Gene4650, were identified, which were mainly participated in C-S cleavage, S-N hydrolysis and isoxazole ring cleavage. Interestingly, we found the corresponding sulfonamides resistance genes were not detected in F6a, which may provide an evidence for low abundance of the sulfonamides resistance genes in BICW system. These findings would contribute to a better understanding of biotransformation of antibiotic in the BICW.

Perfluorohexanoic acid caused disruption of the hypothalamus-pituitary-thyroid axis in zebrafish larvae.

Perfluorohexanoic acid (PFHxA) has been recognized as an alternative to the wide usage of perfluorooctanoate (PFOA) and perfluorooctane sulfonate (PFOS) in the fluoropolymer industry for years. PFHxA has been frequently detected in the environment due to its wide application. However, the ecological safety of PFHxA, especially its toxicological effects on aquatic organisms, remains obscure. In the present study, PFHxA at different concentrations (0, 0.48, 2.4, and 12 mg/L) was added to the culture medium for zebrafish embryo/larval exposure at 96 h postfertilization (hpf). Zebrafish larvae showed a slow body growth trend and changes in thyroid hormone levels (THs) upon PFHxA exposure, indicating the interference effect of PFHxA on fish larval development. Moreover, the transcription levels of genes related to the hypothalamic-pituitary-thyroid (HPT) axis were also analyzed. The gene expression level of thyroid hormone receptor ß (trß) was upregulated in a dose-dependent manner. Exposure to 0.48 mg/L PFHxA increased the expression levels of the thyrotrophic-releasing hormone (trh) and thyroid hormone receptor α (trα). Significant increases in corticotrophin-releasing hormone (crh) and transthyretin (ttr) gene expression were also observed when the zebrafish larvae were treated with 12 mg/L PFHxA, except iodothyronine deiodinases (dio1), which decreased obviously at that point. There were significant declines in the transcription of both thyroid-stimulating hormone ß (tshß) and uridinediphosphate-glucuronosyltransferase (ugt1ab) upon exposure to 2.4 mg/L PFHxA. In addition, PFHxA induced a dose-related inhibitory effect on the transcription of sodium/iodide symporter (nis). Finally, the thyroid status will be destroyed after exposure to PFHxA, thus leading to growth impairment in zebrafish larvae.

Investigation of Measurement Accuracy of Bridge Deformation Using UAV-Based Oblique Photography Technique.

This paper investigates the measurement accuracy of unmanned aerial vehicle-based oblique photography (UAVOP) in bridge deformation identifications. A simply supported concrete beam model was selected and measured using the UAVOP technique. The influences of several parameters, such as overall flight altitude (h), local shooting distance (d), partial image overlap (λ), and arrangement of control points, on the quality of the reconstructed three-dimensional (3D) beam model, were presented and discussed. Experimental results indicated that the quality of the reconstructed 3D model was significantly improved by the fusion overall-partial flight routes (FR), of which the reconstructed model quality was 46.7% higher than those with the single flight route (SR). Despite the minimal impact of overall flight altitude, the reconstructed model quality prominently varied with the local shooting distance, partial image overlap, and control points arrangement. As the d decreased from 12 m to 8 m, the model quality was improved by 48.2%, and an improvement of 42.5% was also achieved by increasing the λ from 70% to 80%. The reconstructed model quality of UAVOP with the global-plane control points was 78.4% and 38.4%, respectively, higher than those with the linear and regional control points. Furthermore, an optimized scheme of UAVOP with control points in global-plane arrangement and FR (h = 50 m, d = 8 m, and λ = 80%) was recommended. A comparison between the results measured by the UAVOP and the total station showed maximum identification errors of 1.3 mm. The study's outcomes are expected to serve as potential references for future applications of UAVOP in bridge measurements.

Nitrogen removal from summer to winter in a field pilot-scale multistage constructed wetland-pond system.

A pilot-scale multistage constructed wetland-pond (MCWP) system with a "pre-ecological oxidation pond, two-stage horizontal subsurface flow constructed wetland (HSCW) and surface flow constructed wetland (SFCW) as the core and postsubmerged plant pond" as the process was used to treat actual polluted river water in the field, and the variation in nitrogen removal from summer to winter was investigated. The results showed that the average total nitrogen (TN) removal efficiency in the MCWP was approximately 40.74%. The significant positive correlation between the daily highest temperature and the TN removal efficiency of the whole system was fitted with a nonlinear curve (R2 = 0.7192). The TN removal load rate in the HSCWs was 2.7-3.7 times that in the SFCW. The SFCW, which had high-density plants (35 plants/m2), increased the proportion of nitrogen removed by plant harvesting and microbial function. The TN transformed by Iris pseudacorus L. accounted for 54.53% in the SFCW. Furthermore, bacteria completed the nitrogen cycle in the SFCW through a variety of nitrogen removal pathways. This research not only investigated the TN removal performance in an MCWP system but also made it possible to predict the TN removal efficiency according to the daily highest temperature from summer to winter in the field.

Effect of garlic essential oil in 97 patients hospitalized with covid-19: A multi-center experience.

To observe the synergistic effect of garlic essential oil in patients with novel coronavirus disease (COVID-19), in addition to the routine treatment, we used garlic essential oil in COVID-19 patients with mild to moderate symptoms and compared their results to those of patients who did not receive the essential oil. We conducted a quasi-experimental study with COVID-19 patients from 3 hospitals. In the experimental group, 97 patients received garlic essential oil combined with conventional treatment. In the control group, 100 patients received only the conventional treatment for COVID-19. The effectiveness and safety of the garlic essential oil were assessed. Compared to the control group, the group receiving garlic essential oil showed a shorter duration of symptoms, shorter time to negative nucleic acid testing (NAT) results and shorter time to improvement on the computed tomography (CT). In the same period, the experimental group showed an increase in the rate of the disappearance of symptoms and the improvement rates of NAT and CT. Due to its effectiveness and safety in patients with COVID-19, garlic essential oil is recommended as a preventive measure or a supportive therapy during the COVID-19 pandemic.

Evaluation of the acute toxicity and neurodevelopmental inhibition of perfluorohexanoic acid (PFHxA) in zebrafish embryos.

Perfluorohexanoic acid (PFHxA), a widely used emerging alternative for 8-carbon PFAAs, has been detected at a high level in the water environment. While its toxicity and environmental health risk are still largely unknown in aquatic life. The present study aimed to evaluated the possible developmental neurotoxicity induced by PFHxA exposure (0, 0.48, 2.4, and 12 mg/L for 120 h) in the zebrafish embryo. Here, both developmental endpoints, neurotransmitters concentrations, locomotor behavior were analyzed. No significant effects on mortality, malformation rate, and growth delay were detected in the low dose treatment groups except for in the high dose group (12 mg/L). A significant increase in swimming speed were noted in the 0.48 mg/L group. Other changes including neurotransmitters concentrations and green fluorescent protein (GFP) expression in Tg (HuC-GFP) zebrafish larvae were significantly increased in 12 mg/L group. Beyond that, genes related to neurodevelopment were significantly decreased in larvae. Moreover, downregulations of protein expression levels of α1-tubulin, elavl3, and gap43 were identified. These results demonstrate that the PFAAs alternative PFHxA have no significant neurodevelopmental effects on zebrafish larvae under acute low-dose exposure, while, it is important to note that PFHxA perform inhibiting effects on neurotransmitter and central nervous system under a relatively high dose. This in vivo study could provide reliable toxicity information for risk assessments of PFHxA on aquatic ecosystems. CAPSULE: PFHxA have no significant neurodevelopmental effects on zebrafish larvae under acute low-dose exposure, while exposed with relatively high-dose, could induced the alternations of neurotransmitter concentrations as well as the genes involved in the early developmental stages of zebrafish, leading to the impairment of the nervous system in zebrafish larvae.

Performance and bacterial community dynamics of hydroponically grown Iris pseudacorus L. during the treatment of antibiotic-enriched wastewater at low/normal temperature.

Antibiotics are widely detected in the water environment, posing a serious threat to the health of humans and animals. The effect of levofloxacin (LOFL) on pollutant removal and the difference in the influence mechanisms at normal and low temperatures in constructed wetlands are worth discussing. A hydroponic culture experiment was designed with Iris pseudacorus L. at low and normal temperatures. LOFL (0-100 µg/L) was added to the systems. The results indicated that the removal of pollutants was affected most by temperature, followed by LOFL concentration. At the same concentration of LOFL, the pollutant removal rate was significantly higher at normal temperature than at low temperature. Low concentrations of LOFL promoted the degradation of pollutants except TN under normal-temperature conditions. Compared with the results at low temperature, the bacterial community richness was higher and the diversity of bacterial communities was lower under normal-temperature conditions. The genera and the function of bacteria were greatly affected by antibiotic concentration, temperature and test time. A series of microorganisms resistant to antibiotics and low temperature were identified in this study. The results will provide valuable information and a reference for our understanding of the ecological effects of LOFL.

The application of steel slag in a multistage pond constructed wetland to purify low-phosphorus polluted river water.

To investigate the effect of a constructed wetland (CW) with steel slag as the filler on water contaminated by low phosphorus levels, a multistage pond CW system was designed in this study. Low-phosphorus polluted river water was used as the research object. This study explored the effects of using steel slag as a CW filler on phosphorus removal and the total phosphorus (TP) purification effect of the wetland system. The results showed that the TP removal rates in the ecological pond, oxidation pond, surface flow wetlands and submerged plant pond were 5.17%, 8.02%, 21.56%, and 16.31%, respectively. Intermittent increases in phosphorus concentration were observed in the reactors and were caused by the decay of plant tissues, which released pollutants. Because steel slag was added to the filler, the TP concentrations in the effluent of the first- and second-level horizontal subsurface CWs increased by 151.13% and 16.29%, respectively, compared to the influent concentration. The 20th to 40th days of the test run was a period of rapid phosphorus release of the system. The use of steel slag has a potential risk of phosphorus release when applied in CWs used to purify low-phosphorus contaminated water bodies.

Response of Cyperus involucratus to sulfamethoxazole and ofloxacin-contaminated environments: Growth physiology, transportation, and microbial community.

Plant-microbe is a complementary coupling system for antibiotics removing in constructed wetlands (CWs), but how plant and rhizosphere microbiomes respond to antibiotics exposure and the occurrence of ARGs in this microenvironment have seldom been researched. Thus, the response of the plant-microbe coupling system to different levels of antibiotics (sulfamethoxazole (SMZ) and ofloxacin (OFL)) was investigated. The results showed that two antibiotic stressors have hormetic effects on plant growth, physiology, and microbial community evolution, and the antibiotic toxic effects presented as SMZ + OFL > SMZ > OFL. Antibiotic accumulation in the plants was in the order of roots > stems > leaves. Notably, the root attachments affected antibiotic transportation. The accumulation of antibiotics in the under-ground parts affected the rhizosphere microbial community structure, and the microorganisms were more sensitive to SMZ + OFL than the plants, with inflection points of 0.5 mg L-1 and 1 mg L-1, respectively. Pseudomonas was highly resistant to antibiotics, while Acidovorax and Devosia may play a role in antibiotic degradation. Correlation analysis and network analysis showed that antibiotic enrichment and the bacterial community contributed significantly to the abundance of antibiotic-resistant genes (ARGs), further revealing the co-occurrence of int1, ARGs, and the potential bacterial hosts.

Occurrence of typical antibiotics and source analysis based on PCA-MLR model in the East Dongting Lake, China.

The antibiotics residues in freshwater lakes are being highlighted around the world because of high potential threat to environment and human health. Understanding the current state of antibiotics and potential sources in lakes are important. The potential sources of antibiotics (Sewage treatment plants (STPs)), livestock and poultry farms and fishponds in the East Dongting was studied. Compared with other surface water bodies, the concentration of antibiotic in the East Dongting Lake was at a moderate or low level. Ten of 12 antibiotics likely originated from veterinary applications in livestock and poultry farms, especially in swine farms, and concentrations at these sources (ND-1240.41 ng L-1) were 1-3 orders of magnitude higher than in the effluent of local sewage treatment plants and fishponds. Based on a principal component analysis with multiple linear regression (PCA-MLR) model, we estimated source contributions of 79.95% for livestock and poultry farms, 0.27% for STPs, and 19.79% for aquaculture source and livestock and poultry farms. Overall, the predominance of sulfonamides and livestock and poultry farms in the East Dongting Lake has been identified, which can provide important information for regulating their veterinary use and environmental management.

Isotopic fractionation of particulate organic matter and its biogeochemical implication in the littoral zone of Lake Taihu, China.

Signatures of stable isotope ratios have previously been used to trace the source and transport of particulate organic matter (POM) in freshwater and marine ecosystems. In this study, water columns were collected at 22 sites in the littoral zone of Lake Taihu in 2014 to investigate the distribution and concentration of nutrients and the stable isotope signatures of POM, and their potential interrelation. Generally, mean concentration of nitrogen forms (NH4+-N) showed substantial variation, probably because they had received large amounts of wastewater from various local industrial enterprises. Source analysis by stable carbon and nitrogen isotopic ratios showed that the main POM sources were phytoplankton. Contrasting correlations were obtained between δ15N and N concentrations in effluent river mouths when compared with influent river mouths. In effluent river mouths, there was a significant positive correlation between δ15N and nitrogen concentration (total nitrogen and ammonia-nitrogen), in contrast with the negative correlation in influent river mouths. According to these results, more factors should be taken into consideration when stable carbon and nitrogen isotopes of POM are used to assess the feeding relationship between consumers and prey, as well as the energy flow pathways that support the lake pelagic food webs.

ELS1, a novel MATE transporter related to leaf senescence and iron homeostasis in Arabidopsis thaliana.

The multidrug and toxic compound extrusion (MATE) transporters mediate the coupled exchange of organic substrates and monovalent cations have been recently implicated in various plant biological activities. In this work, we isolated a dominant mutant from an Arabidopsis activation-tagging mutant pool. This mutant exhibits pleiotropic phenotype including early flowering, dwarf and bushy architecture, minified lateral organs and early leaf senescence, and is therefore designated early leaf senescence 1-Dominaint (els1-D). Genotyping assays showed that els1-D is a gain-of-function mutant of a novel MATE transporter gene, ELS1, which encodes a close homolog of the previously reported ADP1, BCD1 and DTX50. Further investigations revealed that the overexpression of ELS1 reduces iron content in els1-D, and the accelerated senescence of the detached els1-D leaves can be recovered by exogenous iron supply. In addition, we also found that ELS1 is an iron responsive gene. Based on these findings, we proposed that ELS1 is related to leaf senescence and iron homeostasis in Arabidopsis.

The role of GSH in microcystin-induced apoptosis in rat liver: Involvement of oxidative stress and NF-κB.

Microcystins (MCs) are potent and specific hepatotoxins produced by cyanobacteria in eutrophic waters, representing a health hazard to animals and humans. The objectives of this study are to determine the relationship between oxidative stress and NF-κB activity in MC-induced apoptosis in rat liver and the role of glutathione (GSH). Sprague-Dawley rats were intraperitoneally (i.p.) injected with microcystin-LR (MC-LR) at 0.25 and 0.5 LD50 with or without pretreatment of buthionine-(S,R)-sulfoximine (BSO), a specific GSH synthesis inhibitor. MC-LR induced time-dependent alterations of GSH levels in rat liver. Increased malondialdehyde (MDA) and significant changes of antioxidant enzymes including GSH peroxidase (GPX) and GSH reductase (GR) were also observed, particularly at 24 h post-exposure. The results indicated that acute exposure to MC-LR induced oxidative stress, and GSH depletion (BSO pretreatment) enhanced the level of oxidative stress. Furthermore, the modulation of pro-apoptotic gene p53 and Bax and anti-apoptotic gene Bcl-2 was observed in 0.5 LD50 group at 24 h, and the alteration was more pronounced by BSO injection before MC-LR treatment, suggesting that GSH played a protective role against MC-induced toxicity. Additionally, electrophoretic mobility shift assay (EMSA) showed that NF-κB was induced at 0.25 LD50 but inhibited at 0.5 LD50 . The above results indicated that the possible crosstalk of oxidative stress and NF-κB activity was associated with MC-LR-induced hepatocytes apoptosis in vivo. Our data will provide a new perspective for understanding the mechanisms of MC-induced liver injury.

The role of glutathione detoxification pathway in MCLR-induced hepatotoxicity in SD rats.

In the present study, we investigated the role of glutathione (GSH) and its related enzymes in Sprague Dawley (SD) rats subjected to microcystin-leucine-arginine (MCLR)-induced hepatotoxicity. SD rats were intraperitoneally (i.p.) injected with MCLR after pretreating with or without buthionine-(S,R)-sulfoximine (BSO), an inhibitor of GSH synthesis. The depletion of GSH with BSO enhanced MCLR-induced oxidative stress, resulting in more severe liver damage and higher MCLR accumulation. Similarly, the contents of malondialdehyde (MDA), total GSH (T-GSH), oxidized GSH (GSSG) and GSH were significantly enhanced in BSO pretreated rats following MCLR treatment. The study showed that the transcription of GSH-related enzymes such as glutathione-S-transferase (GST), γ-glutamylcysteine synthetase (γ-GCS), glutathione reductase (GR) varied in different ways (expect for glutathione peroxidase (GPx), whose gene expression was induced in all treated groups) with or without BSO pretreatment before MCLR exposure, suggesting an adaptative response of GSH-related enzymes at transcription level to combat enhancement of oxidative stress induced by MCLR when pretreated with BSO. These data suggested the tissues with low GSH concentration are highly vulnerable to MCLR toxicity and GSH was critical for the detoxification in MCLR-induced hepatotoxicity in vivo.

Impact of nutritional support on immunity, nutrition, inflammation, and outcomes in elderly gastric cancer patients after surgery.

BACKGROUND: Postoperative rehabilitation of elderly patients with gastric cancer has always been the focus of clinical attention. Whether the intervention by a full-course nutritional support team can have a positive impact on the postoperative immune function, nutritional status, inflammatory response, and clinical outcomes of this special population has not yet been fully verified. AIM: To evaluate the impact of full-course nutritional support on postoperative comprehensive symptoms in elderly patients with gastric cancer. METHODS: This is a retrospective study, including 60 elderly gastric cancer patients aged 70 years and above, divided into a nutritional support group and a control group. The nutritional support group received full postoperative nutritional support, including individualized meal formulation, and intravenous and parenteral nutrition supplementation, and was regularly evaluated and adjusted by a professional nutrition team. The control group received routine postoperative care. RESULTS: After intervention, the proportion of CD4+ lymphocytes (25.3% ± 3.1% vs 21.8% ± 2.9%, P < 0.05) and the level of immunoglobulin G (12.5 G/L ± 2.3 G/L vs 10.2 G/L ± 1.8 G/L, P < 0.01) were significantly higher in the nutritional support group than in the control group; the changes in body weight (-0.5 kg ± 0.8 kg vs -1.8 kg ± 0.9 kg, P < 0.05) and body mass index (-0.2 ± 0.3 vs -0.7 ± 0.4, P < 0.05) were less significant in the nutritional support group than in the control group; and the level of C-reactive protein (1.2 mg/L ± 0.4 mg/L vs 2.5 mg/L ± 0.6 mg/L, P < 0.01) and WBC count (7.2 × 109/L ± 1.5 × 109/L vs 9.8 × 109/L ± 2.0 × 109/L, P < 0.01) were significantly lower in the nutritional support group than in the control group. In addition, patients in the nutritional support group had a shorter hospital stay (10.3 d ± 2.1 d vs 14.8 d ± 3.6 d, P < 0.05) and lower incidence of infection (15% vs 35%, P < 0.05) in those of the control group. CONCLUSION: The intervention by the nutritional support team has a positive impact on postoperative immune function, nutritional status, inflammatory response, and clinical outcomes in elderly patients with gastric cancer.

Development and trends of constructed wetland substrates over the past 30 years: a literature visualization analysis based on CiteSpace.

Constructed wetland substrates (CWSs) have received considerable attention owing to their importance in adsorbing and degrading pollutants, providing growth attachment points for microorganisms, and supporting wetland plants. There are differences in the configurations and functions of constructed wetlands (CWs) for treating different water bodies and sewage, resulting in a wide variety of substrates. Research on the application and mechanism of CWSs is not sufficiently systematic. Therefore, the current research advancements and hotspots must be identified. Hence, we used CiteSpace to analyze 1955 English publications from the core collection database of the Web of Science to assess the current state of the CWS research field. Based on the cooperative network analysis, the roles of various countries, institutions, and authors in research on CWSs were reviewed. Keyword co-occurrence and cluster analyses were used to discuss the transformation of CWSs from removing traditional pollutants to emerging pollutants and the transition from incorporating natural substrates to artificial substrates. Finally, we underscored the need for more emphasis to be placed on the collocation and application of the CWSs at different latitudes. Furthermore, the substrate micro-interface process and its effects on the interaction patterns of pollutants and microorganisms should be thoroughly investigated to provide theoretical guidance for the development of wetland applications and mechanisms.

Disclosing the intrinsic nature of efficient removal of antibiotics in N/S dual-doped porous carbon-based materials: The manipulation of internal electric field.

N/S co-doping has emerged as a prevailing strategy for carbon-based adsorbents to facilitate the antibiotic removal efficiency. Nevertheless, the underlying interplay among N, S, and their adjacent vacancy defects remains overlooked. Herein, we present a novel in situ strategy for fabricating pyridinic-N dominated and S dual-doped porous carbon adsorbent with rich vacancy defects (VNSC). The experimental results revealed that N (acting as the electron donor) and S (acting as the electron acceptor) form an internal electric field (IEF), with a stronger IEF generated between pyridinic-N and S, while their adjacent vacancy defects activate carbon π electrons, thus enhancing the charge transfer of the IEF. Density functional theory (DFT) calculations further demonstrated that the rich charge transfer in the IEF facilitated the π-π electron donor-acceptor (EDA) interaction between VNSC and tetracycline (TC) as well as norfloxacin (NOR), and thus is the key to adsorption performance of VNSC. Consequently, VNSC exhibited high adsorption capacities toward TC (573.1 mg g-1) and NOR (517.0 mg g-1), and its potential for environmental applications was demonstrated by interference, environmentally relevant concentrations, fixed-bed column, and regeneration tests. This work discloses the natures of adsorption capacity for N/S dual-doped carbon-based materials for antibiotics.