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The factors predicting the progression of coronavirus disease-2019 (COVID-19) from mild to moderate to critical are unclear. We retrospectively evaluated risk factors for disease progression in Japanese patients with COVID-19. Seventy-four patients with laboratory-confirmed COVID-19 were hospitalized in our hospital between February 20, 2020, and June 10, 2020. We excluded asymptomatic, non-Japanese, and pediatric patients. We divided patients into the stable group and the progression group (PG; requiring mechanical ventilation). We compared the clinical factors. We established the cutoff values (COVs) for significantly different factors via receiver operating characteristic curve analysis and identified risk factors by univariate regression. We enrolled 57 patients with COVID-19 (median age 52 years, 56.1% male). The median time from symptom onset to admission was 8 days. Seven patients developed critical disease (PG: 12.2%), two (3.5%) of whom died; 50 had stable disease. Univariate logistic analysis identified an elevated lactate dehydrogenase (LDH) level (COV: 309 U/l), a decreased estimated glomerular filtration rate (eGFR; COV: 68 ml/min), lymphocytopenia (COV: 980/µl), and statin use as significantly associated with disease progression. However, in the Cox proportional hazards analysis, lymphocytopenia at admission was not significant. We identified three candidate risk factors for progression to critical COVID-19 in adult Japanese patients: statin use, elevated LDH level, and decreased eGFR.
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COVID-19/diagnóstico , Estado Terminal , Adulto , Idoso , Biomarcadores , COVID-19/epidemiologia , Progressão da Doença , Feminino , Humanos , Japão , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Fatores de RiscoRESUMO
Seven South Pacific anguillid eel species live from New Guinea to French Polynesia, but their spawning areas and life histories are mostly unknown despite previous sampling surveys. A July-October 2016 research cruise was conducted to study the spawning areas and times, and larval distributions of South Pacific anguillid eels, which included a short 155°E station-line northeast of New Guinea and five long transects (5-25°S, 160°E-140°W) crossing the South Equatorial (SEC) and other currents. This survey collected nearly 4000 anguilliform leptocephali at 179 stations using an Isaacs-Kidd Midwater Trawl accompanied by 104 CTD casts. Based on mor-phometric observations and DNA sequencing, 74 anguillid leptocephali were collected, which in the southern areas included 29 larvae of six species: Anguilla bicolor pacifica, A. marmorata, A. australis, A. reinhardtii, A. megastoma, and A. obscura (all anguillid species of the region were caught except A. dieffenbachii). Small A. australis (9.0-16.8 mm) and A. reinhardtii (12.4, 12.5 mm) leptocephali were collected south of the Solomon Islands, other A. australis (10.8-12.0 mm) larvae were caught northwest of Fiji along with an A. obscura (20.0 mm) larva, and an A. marmorata (7.8 mm) larva was collected near Samoa. Considering collection sites, larval ages from otolith analysis, and westward SEC drift, multiple spawning locations occurred from south of the Solomon Islands and the Fiji area (16-20 days old larvae) to near Samoa (19 days old larva) during June and July in areas where high-salinity Subtropical Underwater (STUW, ~150 m depth) and the warm, low-salinity surface Fresh Pool were present. Five long hydrographic sections showed the strong Fresh Pool in the west and the STUW formation area in the east.
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[Purpose] Ultrasonography (US) is widely applied to measure the muscle size in the limbs, as it has relatively high portability and is associated with low costs compared with large clinical devices such as magnetic resonance imaging (MRI). However, the applicability of US for evaluating trunk muscle size is poorly understood. This study aimed to examine whether US-measured muscle thickness (MT) in the trunk abdominal and back muscles correlated with MT and muscle cross-sectional area (MCSA) measured by MRI. [Subjects and Methods] Twenty-four healthy young males participated in this study. The MT and MCSA in the subjects were measured by US and MRI in a total of 10 sites, including the bilateral sides of the rectus abdominis (upper, central, and lower parts), abdominal wall, and multifidus lumborum. [Results] The interclass correlation coefficients of US-measured MT on the total 10 sites showed excellent values (n=12, 0.919 to 0.970). The US-measured MT significantly correlated with the MRI-measured MT (r=0.753 to 0.963) and MCSA (r=0.634 to 0.821). [Conclusion] US-measured MT could represent a surrogate for muscle size measured by MRI. The application of US for evaluating trunk muscle size may be a useful tool in the clinical setting.
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[Purpose] Recently, we demonstrated that the thicknesses of trunk muscles measured using ultrasonography were correlated strongly with the cross-sectional areas measured using magnetic resonance imaging in untrained subjects. To further explore the applicability of ultrasonography in the clinical setting, the present study examined the correlation between ultrasonography-measured thicknesses and magnetic resonance imaging-measured cross-sectional areas of trunk muscles in athletes with trained trunk muscles. [Subjects and Methods] The thicknesses and cross-sectional areas at total 10 sites of the bilateral sides of the upper, central, and lower parts of the rectus abdominis, abdominal wall, and multifidus lumborum in 30 male baseball batters were measured. [Results] Overall thicknesses and cross-sectional areas of the trunk muscles in baseball batters were higher than those in untrained subjects who participated in our previous study. The ultrasonography-measured thicknesses at all 10 sites of the trunk muscles correlated highly with the magnetic resonance imaging-measured cross-sectional areas in baseball batters. [Conclusion] These results suggest that the thicknesses of the trunk muscles measured using ultrasonography can be used as a surrogate marker for the cross-sectional area measured using magnetic resonance imaging, in athletes who have larger trunk muscles than that of untrained subjects.
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Although the lift force (F(L)) on a spinning baseball has been analyzed in previous studies, no study has analyzed such forces over a wide variety of spins. The purpose of this study was to describe the relationship between F(L) and spin for different types of pitches thrown by collegiate pitchers. Four high-speed video cameras were used to record flight trajectory and spin for 7 types of pitches. A total of 75 pitches were analyzed. The linear kinematics of the ball was determined at 0.008-s intervals during the flight, and the resultant fluid force acting on the ball was calculated with an inverse dynamics approach. The initial angular velocity of the ball was determined using a custom-made apparatus. Equations were derived to estimate the F(L) using the effective spin parameter (ESp), which is a spin parameter calculated using a component of angular velocity of the ball with the exception of the gyro-component. The results indicate that F(L) could be accurately explained from ESp and also that seam orientation (4-seam or 2-seam) did not produce a uniform effect on estimating F(L) from ESp.
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Aceleração , Beisebol , Reologia/métodos , Rotação , Equipamentos Esportivos , Estresse Mecânico , Ar , Simulação por Computador , Fricção , Modelos TeóricosRESUMO
We investigated the interaction between relaxation and contraction for remote, ipsilateral muscles of the hand and foot (wrist extensor/ankle dorsiflexor). Subjects sat in an armchair and were able to freely move their right hand and foot. They performed eight tasks: (1) wrist extension from a flexed (relaxed) position, (2) ankle dorsiflexion from a plantarflexed (relaxed) position, (3) wrist relaxation from an extended position, (4) ankle relaxation from a moderately dorsiflexed position. The remaining tasks involved (5) simultaneous performance of tasks 1 and 2, (6) 3 and 4, (7) 1 and 4, and (8) 2 and 3. Subjects performed each task as fast as possible after hearing a start signal. They were instructed not to activate antagonistic muscles in the tasks involving relaxation. When contraction of the wrist flexor/ankle dorsiflexor was concurrent with relaxation of the ipsilateral limb (ankle dorsiflexor/wrist extensor), the reaction time of contraction as observed in electromyography (EMG) activities in the wrist extensor/ankle dorsiflexor became longer and the EMG activities became smaller. Our findings suggest that muscle relaxation in one limb interferes with smooth movement of muscle contraction in the ipsilateral limb.
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Pé/fisiologia , Movimento/fisiologia , Contração Muscular/fisiologia , Relaxamento Muscular/fisiologia , Punho/fisiologia , Adulto , Tornozelo/fisiologia , Eletromiografia/métodos , Feminino , Humanos , Masculino , Adulto JovemRESUMO
The purpose of this study was to examine (a) the acute change in bat velocity (BV) following three types of warm-up procedures for baseball hitting (experiment 1), and (b) the effect of an 8-week training program of isometric contraction conditioning (ISO) on BV (experiment 2). In experiment 1, the BV of 24 collegiate baseball players was measured before and after one of the three warm-up procedures; five standard bat (mass = 850.5 g) dry swings (SBS), five weighted bat (mass = 850.5 g + 680.4 g) dry swings (WBS), and four sets of 5-second maximal voluntary isometric contractions mimicking the bat swing movement pattern (ISO). BV was measured just before ball-bat impact. Experiment 2 followed experiment 1 and used only the ISO warm-up. Twelve of the 24 subjects formed the experimental group and underwent an 8-week training program (3 days per week) of ISO training. Results of experiment 1 indicated (a) no significant change in post-SBS BV (-0.33 m·s-¹), (b) a significant decrease in post-WBS BV (-0.89 m·s-¹; p < 0.05), and (c) a significant increase in post-ISO BV (+0.39 m·s-¹; p < 0.05). In experiment 2, there was a significant increase in baseline BV after the 8-week training period (30.21 ± 1.83 m·s -¹ to 31.15 ± 1.57 m·s-¹). A comparison of BV before and after ISO warm-up revealed that change was significantly greater after the training period (100.17 ± 3.18% vs. 103.75 ± 1.91%). Our results suggest that warm-up with WBS does not increase BV and that using the ISO has both acute and chronic positive effects on BV as a warm-up procedure to improve BV.
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Desempenho Atlético/fisiologia , Beisebol/fisiologia , Contração Isométrica/fisiologia , Educação Física e Treinamento/métodos , Equipamentos Esportivos , Análise de Variância , Humanos , Masculino , Adulto JovemRESUMO
The present study investigated the relationship of performance variables and anthropometric measurements on baseball ability in 164 youth baseball players (age: 6.4-15.7 years). To evaluate their baseball performance, ball speeds in pitching and batting were recorded and kinetic energies of the pitched and hit balls were calculated. To record anthropometric and physical fitness characteristics, height and weight were measured and a battery of physical fitness tests covering standing long jump, side steps, sit-ups, 10-m sprint, trunk flexion, back strength, and grip strengths of both hands were conducted. The results of a multiple regression analysis revealed several significant predictors: age, body mass index (BMI), standing long jump, 10-m sprint, and grip strength for pitched ball kinetic energy and age, BMI, standing long jump, and back strength for hit ball kinetic energy. This study provides scientific evidence that relates certain specific physical performance tests and body characteristics with high achievement in the actual performance of pitching and batting. Youth players, their parents, coaches, and trainers would benefit by addressing these characteristics when planning training programs to improve the baseball performance of youth players.
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Desempenho Atlético/fisiologia , Beisebol/fisiologia , Adolescente , Antropometria , Fenômenos Biomecânicos , Criança , Humanos , Masculino , Aptidão Física/fisiologiaRESUMO
The effectiveness of fastballs of equivalent speed can differ; for example, one element of this difference could be due to the effect of rate and orientation of ball spin on launched ball trajectory. In the present experiment, baseball batters' accuracy in hitting fastballs with different backspin rates at a constant ball velocity of 36 m/s was examined. Thirteen skilled baseball players (professionals, semiprofessionals, and college varsity players) participated in the study. The movements of bat and ball were recorded using two synchronized high-speed video cameras. The Pearson product-moment correlation coefficient (r) was calculated and used to analyze the relationship between ball backspin rate and the vertical distance between ball center and sweet spot at the moment of ball-bat impact. Ball backspin rate was positively correlated with increases in the distance from the optimal contact point of the swung bat (sweet spot) to the actual point of contact (r = .38, P < .001). Batters were most effective at the usual backspin rate for the ball velocity used. The decrease in accuracy of the batter's swing that was observed when the fastball's backspin deviated from the usual rate likely occurred because experienced batters predict ball trajectory from perceived ball speed.
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Desempenho Atlético/fisiologia , Beisebol/fisiologia , Equipamentos Esportivos , Extremidade Superior/fisiologia , Aceleração , Fenômenos Biomecânicos , Humanos , Masculino , Gravação em Vídeo , Adulto JovemRESUMO
The present study investigated bat control of skilled baseball batters during tee batting, faced with an intentional change in target position. Twelve, skilled, male baseball players (M age = 24 yr., SD = 4) participated in the study. Participants were instructed to hit a baseball off a tee 0.8 m from the ground (Hitting condition), and also to deliberately swing just above the ball (Air Swing condition). The task for the participants was to perform, in alternate order, 15 swings at a real baseball on a stationary tee and 15 swings at an imaginary ball that was said to be on top of the real baseball. The participants were instructed to hit the ball toward center field just as they would hit in a game. While most participants could hit the real ball in the sweet area of the bat, only one participant did so in the Air Swing condition. Average distances from the center of the sweet area to ball center at the moment of ball-bat contact in the Air Swing condition (85 mm) were significantly greater than the distance in the Hitting condition (38 mm). The larger error in hitting an imaginary ball in the sweet area could be due to perceptual changes following the batter's altered focus, expectation of a lack of contact, and/or lack of feedback from the swing. It was suggested that baseball batters should be aware of the possible error in hitting accurately when they intentionally shifted the target.
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Desempenho Atlético/fisiologia , Beisebol/fisiologia , Teste de Esforço/métodos , Adulto , Objetivos , Humanos , Intenção , Masculino , Adulto JovemRESUMO
The tropical Celebes eel, Anguilla celebesensis, has a short migration between its spawning and growth habitats. Its spawning areas were hypothesized to be in Tomini Bay and the Celebes Sea after collecting their small leptocephali. However, there is no information about the silver eel oceanic spawning migration behavior of A. celebesensis. To better understand their short-distance spawning migration behavior, four large female silver eels (Eel 1-4) were equipped with pop-up satellite archival tags (PSATs) and released near the mouth of the Poso River in Tomini Bay of Sulawesi Island on 22 February (Eel 1-3) and 11 March 2010 (Eel 4). All PSATs ascended in Tomini Bay and transmitted their data. Eel 3 and 4 provided clear records of consistent diel vertical migration (DVM: eight days-Eel 3, 13 days-Eel 4) with daytime dives to mean depths of 444.7 m (Eel 3) and 539.0 m (Eel 4), where mean temperatures were 9.1°C (Eel 3) and 7.7°C (Eel 4), and nighttime ascents to mean depths of 132.8 m (Eel 3) and 112.4 m (Eel 4), where mean temperatures were 20.6°C (Eel 3) and 23.4°C (Eel 4). Eel 3 and 4 started to dive to deeper water around nautical dawn and swam up to shallower water around sunset. During nighttime, both eels swam in deeper and colder water during nights with moonlight than during nights without moonlight, and there was a negative linear relationship between experienced water temperatures with the moon in the sky and the lunar age for the eels. The A. celebesensis daily rhythm of DVM behaviors was similar to spawning-migration DVM behaviors of other anguillid species. Essential life history characteristics of A. celebesensis appear to be a short migration between freshwater growth habitat and ocean spawning habitat, and high GSI values with advanced gonadal development in downstream-migrating silver eels.
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To understand the spawning ecology of the Japanese eel, the spawning time of this species was estimated based on measurements of the ascending speed of eggs and previously obtained data. Two types of water temperature parameters were calculated assuming an arbitrary spawning time. The 'incubation temperature' of 53 eggs collected in the spawning area was estimated based on the developmental stage of each egg and experimentally determined relationships between water temperature and incubation duration. The 'experienced temperature' of eggs ascending in the water column after spawning was estimated based on an ascending egg speed of 3.69 m/h and spawning depth of 230 m determined from a pop-up satellite archival tag release experiment on silver eels conducted in the same area. The incubation and experienced temperatures of the eggs coincided only at 20:20-22:30 h, 3 days prior to the new moon. This period is only a few hours after the diel vertical migration of Japanese eels in the evening, when adults move up from a depth of ~800 m (approximately 5 °C) to shallower waters of 200-250 m depth (approximately 20 °C). Our findings will facilitate improvements in aquaculture techniques and the detection of eel spawning events in the open ocean.
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Anguilla/fisiologia , Migração Animal/fisiologia , Animais , Oceanos e Mares , Reprodução/fisiologia , TemperaturaRESUMO
Successful baseball hitting involves a combination of highly trained perceptual skills and forceful bat swing motions. The purpose of the present study was to quantify the horizontal movement of the head and eyes while baseball batters hit a fastball to clarify a visual strategy for this highly trained interceptive task. Six collegiate baseball players hit a fastball that was launched from a pitching machine. The ball speed was 31.9 m·s-1 for the Slow Ball Task and 40.3 m·s-1 for the Fast Ball Task. Horizontal head movements were analysed using images that were captured by two high-speed video cameras. The Horizontal eye movement was recorded with electrooculography. The angular speed of the horizontal head and eye movements during hitting were divided into four time periods (I-40 = 21-40% of total ball-flight, I-60 = 41-60% of total ball-flight, I-80 = 61-80% of total ball-flight, I-100 = 81-100% of total ball-flight) and analysed using analysis of variance and a Tukey post-hoc multiple-comparison. In the Slow Ball Task, the horizontal angular velocity of the head during I-80 was significantly faster than that during I-40 (p < 0.05). In the Fast Ball Task, the horizontal angular velocity of the head during I-80 was significantly faster than that during I-40 and I-60 (p < 0.05). These results indicated that the tracking motion of the head became faster as the launched ball came close to the batters, but there was no change in the angular tracking motion of the eyes. Therefore, rapid eye movement may not be suitable to accurately estimate the ball's future location during fastball hitting based on the eye-centered coordinates. Our findings suggest that conventional vision training with a wide range of saccadic or smooth-pursuit eye movements does not reflect the characteristics of tracking strategies during baseball hitting.
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Desempenho Atlético , Beisebol , Movimentos Oculares , Movimentos da Cabeça , Destreza Motora , Atletas , Desempenho Atlético/fisiologia , Beisebol/fisiologia , Fenômenos Biomecânicos , Movimentos Oculares/fisiologia , Lateralidade Funcional , Movimentos da Cabeça/fisiologia , Humanos , Destreza Motora/fisiologia , Adulto JovemRESUMO
Takatoshi Higuchi, Shun Watanabe, Ryotaro Manabe, Tsuyoshi Kaku, Akihiro Okamura, Yoshiaki Yamada, Michael J. Miller, and Katsumi Tsukamoto (2018) Japanese eels Anguilla japonica were tagged in order to understand their behavior in their spawning area. Three silver eels (EEL-A, B, C: TL792, 898, 992 mm) were tagged with pop-up satellite archival transmitting tags (PSATs) and released at different locations near/in their spawning area along the southern part of the West Mariana Ridge. EEL-A showed premature tag pop-up with mostly disordered records and the EEL-C tag did not pop up, while EEL-B showed stable diel vertical migrations during 31 of the 43 days it was tracked. EEL-B swam in shallower layers (411-182 m) during nighttime and deeper layers (563-885 m) during daytime. The mean nighttime swimming depth ± SD of EEL-B was significantly deeper during the full moon (342.4 ± 6.8 m) than the new moon (274.8 ± 16.9 m) and was positively correlated with the moon's altitude. EEL-B reached its maximum depths (851.1 ± 22.8 m) and minimum water temperatures (4.9 ± 0.1°C) during the sun culmination (sun at its highest point in the sky) of each day. The daytime water temperature varied between 4.7 and 5.2°C, staying at an almost constant 5°C. The eel started to dive to deeper water around nautical twilight (sun altitude: -11.6 ± 4.6°) and rise shallower around sunset (sun altitude: -0.8 ± 1.4°); sun altitude and swimming depth were correlated during the dives at dawn and ascents up at dusk. These results suggest that the regular diel vertical migrations of Japanese eels are strictly regulated by both light intensity and the lower limit of water temperature.
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The purpose of the present study was to examine the relationships between bat swing speed (BSS), muscle thickness, and muscle thickness asymmetry in collegiate baseball players. Twenty-four collegiate baseball players participated in this study. Maximum BSS in hitting a teed ball was measured using a motion capture system. The muscle thicknesses of the trunk (upper abdominal rectus, central abdominal rectus, lower abdominal rectus, abdominal wall, and multifidus lumborum), upper limb, and lower limb were measured using a B-mode ultrasonography. Lateral asymmetry between each pair of muscles was determined as the ratio of the thickness of the dominant side to that of the non-dominant side. Statistically significant positive correlations were observed between BSS and muscle thicknesses of the abdominal wall and multifidus lumborum on the dominant side (r = 0.426 and 0.431, respectively; p < 0.05), whereas only trends against this significance were observed between BSS and muscle thicknesses on the non-dominant side. No statistical correlations were found between BSS and the lateral asymmetry of any muscles. These findings indicate the importance of the trunk muscles for bat swing, and the lack of association between BSS and lateral asymmetry of muscle size.
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The contribution of visual information about a pitched ball to the accuracy of baseball-bat contact may vary depending on the part of trajectory seen. The purpose of the present study was to examine the relationship between hitting accuracy and the segment of the trajectory of the flying ball that can be seen by the batter. Ten college baseball field players participated in the study. The systematic error and standardized variability of ball-bat contact on the bat coordinate system and pitcher-to-catcher direction when hitting a ball launched from a pitching machine were measured with or without visual occlusion and analyzed using analysis of variance. The visual occlusion timing included occlusion from 150 milliseconds (ms) after the ball release (R+150), occlusion from 150 ms before the expected arrival of the launched ball at the home plate (A-150), and a condition with no occlusion (NO). Twelve trials in each condition were performed using two ball speeds (31.9 m·s-1 and 40.3 m·s-1). Visual occlusion did not affect the mean location of ball-bat contact in the bat's long axis, short axis, and pitcher-to-catcher directions. Although the magnitude of standardized variability was significantly smaller in the bat's short axis direction than in the bat's long axis and pitcher-to-catcher directions (p < 0.001), additional visible time from the R+150 condition to the A-150 and NO conditions resulted in a further decrease in standardized variability only in the bat's short axis direction (p < 0.05). The results suggested that there is directional specificity in the magnitude of standardized variability with different visible time. The present study also confirmed the limitation to visual information is the later part of the ball trajectory for improving hitting accuracy, which is likely due to visuo-motor delay.
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Desempenho Atlético/fisiologia , Beisebol/fisiologia , Desempenho Psicomotor , Humanos , Fenômenos Mecânicos , Fatores de Tempo , Percepção Visual , Adulto JovemRESUMO
PURPOSE: Dynamic visual acuity (DVA) is defined as the ability to discriminate the fine parts of a moving object. DVA is generally better in baseball players than that in nonplayers. Although the better DVA of baseball players has been attributed to a better ability to track moving objects, it might be derived from the ability to perceive an object even in the presence of a great distance between the image on the retina and the fovea (retinal error). However, the ability to perceive moving visual stimuli has not been compared between baseball players and nonplayers. METHODS: To clarify this, we quantitatively measured abilities of eye movement and visual perception using moving Landolt C rings in baseball players and nonplayers. RESULTS: Baseball players could achieve high DVA with significantly faster eye movement at shorter latencies than nonplayers. There was no difference in the ability to perceive moving object's images projected onto the retina between baseball players and nonplayers. CONCLUSIONS: These results suggest that the better DVA of baseball players is primarily due to a better ability to track moving objects with their eyes rather than to improved perception of moving images on the retina. This skill is probably obtained through baseball training.
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Beisebol/fisiologia , Movimentos Oculares/fisiologia , Acuidade Visual , Percepção Visual/fisiologia , Análise de Variância , Humanos , Masculino , Percepção de Movimento/fisiologia , Visão Ocular/fisiologia , Adulto JovemRESUMO
PURPOSE: In this study, we analyzed the direction of the spin axis angles and the spin rate of baseballs pitched by elite collegiate and professional pitchers. METHOD: The video image of a ball being pitched was taken from the period just before release until 200 ms after release with a high-speed video camera at a rate of 1000 frames per second. A custom-made device was used to analyze the spin axis angle and the spin rate. RESULTS AND CONCLUSION: There were no significant differences in the direction of the spin axis angles or the spin rate between collegiate and professional pitchers. A significant correlation was obtained between spin rate and ball speed; that is, the higher the ball speed, the greater the spin rate. In addition, the spin rate deviated more across subjects than did ball speed. For all subjects, the azimuth and elevation of spin axis were 19° ± 14° and -32° ± 9°, respectively. Some of the pitchers were able to put a characteristic spin on their fastball; the nature of this spin could be related to their pitching success.
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Beisebol/fisiologia , Rotação , Adulto , Atletas , Humanos , Masculino , Análise e Desempenho de Tarefas , Adulto JovemRESUMO
Cystic embryoid bodies with visceral yolk-sac-like structure (cystic EB-Vs) are used as a model for the study of early extraembryonic tissue formation containing visceral endoderm-like derivatives. In this study, we optimized the cell density of embryonic stem (ES) cells for developing cystic EB-Vs in a low-adherence 96-well plate. When ES cells were seeded at a density of 4000 cells/well, the cystic EB-Vs were most efficiently developed from ES cells via forming multicellular spherical aggregates called embryoid bodies (EBs). The suspension culture in the low-adherence plate was preferable for developing EBs into cystic EB-Vs rather than the attachment culture in the plate coated with 0.1% gelatin. The seeding cell density of 4000 cells/well was always superior to 1000 cells/well in the efficiency of cystic EB-V development. Because the high-cell density culture generally raises the limitation of oxygen and nutrient supplies, we investigated the effects of low-oxygen and low-nutrient conditions on the development of cystic EB-Vs. It was found that low oxygen tension was not a factor for promoting the development of cystic EB-Vs. It was suggested that a low-nutrient medium is preferred for developing cystic EB-Vs rather than a sufficient-nutrient medium. In conclusion, the suspension culture in the low-adherence 96-well plate seeded with 4000 ES cells/well was optimum for developing cystic EB-Vs. The low-nutrient condition may be one of the factors for promoting the development of cystic EB-Vs.