Accumulation and distribution of arsenic and cadmium in winter wheat (Triticum aestivum L.) at different developmental stages.

Arsenic (As) and cadmium (Cd) are known to be toxic to humans, and elevated concentrations have been documented in food crops worldwide. However, little is known regarding their uptake, translocation, and distribution in wheat plants during plant development. A series of experiments were conducted to investigate the spatial distribution and dynamics of As and Cd in two wheat cultivars (cv. JN12 and JM85; the latter is a low grain Cd accumulator) at different developmental stages. Root concentrations of As decreased by 84%, and those of Cd by 67%, from tillering to maturity. In contrast, As concentrations in the stems increased 3.1-fold. A significant decrease in root As accumulation was observed at the mature stage, whereas root Cd accumulation decreased largely at the elongation stage. The concentrations of Cd in all leaves and As in new leaves increased as plant growth advanced. However, As concentrations in old leaves decreased significantly from grain filling to maturity. In both cultivars, the upward transfer toward younger parts of shoots was greater in the case of Cd than of As. The remobilization of As and Cd from stems and roots differed between the two cultivars. Arsenic concentrations in rachis, glumes, and grain in JM85 were significantly higher than those in JN12, whereas As concentrations in roots and stems did not differ between the cultivars. Grain Cd was significantly higher in JN12 than in JM85, but Cd concentrations in rachis and glumes were similar between the cultivars. The difference in grain Cd concentration between the two cultivars depended on root and stem Cd remobilization and redistribution from rachis to glumes and grain; in contrast, accumulation of As in grain was influenced by As remobilization from the leaves and stem to the spike.

Phytochelatins play key roles for the difference in root arsenic accumulation of different Triticum aestivum cultivars in comparison with arsenate uptake kinetics and reduction.

In the previous studies, we have found that arsenic (As) accumulation in roots of bread wheat (Triticum aestivum L.) seedlings were significantly different among different wheat cultivars, and As(V) tolerant wheat cultivars have much higher capacities of root As accumulation. However, the reason for the difference remains unclear. Four wheat cultivars with high (MM45 and FM8) or low (QF1 and HM29) levels of arsenic (As) accumulation were selected to investigate the relationship between root As(V) uptake kinetics and root As accumulation. MM45 and HM29 were also used to examine As(V) reduction ability and non-protein thiol (cysteine [Cys], glutathione [GSH], and phytochelatins [PCs]) concentrations in wheat seedlings. MM45 had the lowest Michaelis-Menten constant (Km) and maximum influx rate (Vmax). No difference in the Km values was found among the three other cultivars. No difference in As(V) reduction capacity was observed between MM45 and HM29. GSH and PC2 were significantly induced by 10 µM As(V) in roots of wheat seedlings, particularly in MM45. Synthesis of GSH and PCs was completely suppressed in the presence of l-buthionine sulfoximine (BSO), a specific inhibitor of γ-glutamylcysteine synthetase. BSO markedly decreased the As tolerance of wheat seedlings and decreased the accumulation of As in roots, but increased As accumulation in shoots. No significant difference in As concentrations was found between MM45 and HM29 under the BSO treatment. GSH and PCs are the reason why As accumulation and As(V) tolerance differ in roots of different wheat cultivars.