RESUMO
Tree growth and survival are dependent on their ability to perceive signals, integrate them, and trigger timely and fitted molecular and growth responses. While ectomycorrhizal symbiosis is a predominant tree-microbe interaction in forest ecosystems, little is known about how and to what extent it helps trees cope with environmental changes. We hypothesized that the presence of Laccaria bicolor influences abiotic cue perception by Populus trichocarpa and the ensuing signaling cascade. We submitted ectomycorrhizal or non-ectomycorrhizal P. trichocarpa cuttings to short-term cessation of watering or ozone fumigation to focus on signaling networks before the onset of any physiological damage. Poplar gene expression, metabolite levels, and hormone levels were measured in several organs (roots, leaves, mycorrhizas) and integrated into networks. We discriminated the signal responses modified or maintained by ectomycorrhization. Ectomycorrhizas buffered hormonal changes in response to short-term environmental variations systemically prepared the root system for further fungal colonization and alleviated part of the root abscisic acid (ABA) signaling. The presence of ectomycorrhizas in the roots also modified the leaf multi-omics landscape and ozone responses, most likely through rewiring of the molecular drivers of photosynthesis and the calcium signaling pathway. In conclusion, P. trichocarpa-L. bicolor symbiosis results in a systemic remodeling of the host's signaling networks in response to abiotic changes. In addition, ectomycorrhizal, hormonal, metabolic, and transcriptomic blueprints are maintained in response to abiotic cues, suggesting that ectomycorrhizas are less responsive than non-mycorrhizal roots to abiotic challenges.
Assuntos
Micorrizas , Ozônio , Populus , Micorrizas/fisiologia , Simbiose , Sinais (Psicologia) , Raízes de Plantas/metabolismo , Ecossistema , Populus/genéticaRESUMO
Ozone (O3) is one of the most harmful and widespread air pollutants, affecting crop yield and plant health worldwide. There is evidence that O3 reduces the major limiting factor of photosynthesis, namely CO2 mesophyll conductance (gm), but there is little quantitative information of O3-caused changes in key leaf anatomical traits and their impact on gm. We exposed two O3-responsive clones of the economically important tree species Populus × canadensis Moench to 120 ppb O3 for 21 days. An anatomical diffusion model within the leaf was used to analyse the entire CO2 diffusion pathway from substomatal cavities to carboxylation sites and determine the importance of each structural and subcellular component as a limiting factor. gm decreased substantially under O3 and was found to be the most important limitation of photosynthesis. This decrease was mostly driven by an increased cell wall thickness and length of subcellular diffusion pathway caused by altered interchloroplast spacing and chloroplast positioning. By contrast, the prominent leaf integrative trait leaf dry mass per area was neither affected nor related to gm under O3. The observed relationship between gm and anatomy, however, was clone-dependent, suggesting that mechanisms regulating gm may differ considerably between closely related plant lines. Our results confirm the need for further studies on factors constraining gm under stress conditions.
RESUMO
The CO2 diffusion conductance within the leaf mesophyll (gm) is considered a major limiting factor of photosynthesis. However, the effects of the major secondary air pollutant ozone (O3) on gm have been poorly investigated. Eight genotypes of the economically important tree species Populus × canadensis Moench were exposed to 120 ppb O3 for 21 d. gm showed a genotype-dependent response to O3-induced oxidative stress and was a major limiting factor of net assimilation rate (Anet), ahead of stomatal conductance to CO2 (gsc) and of the maximum carboxylation capacity of the Rubisco enzyme (Vcmax) in half of the tested genotypes. Increased leaf dry mass per area (LMA) and decreased chlorophyll content were linked to the observed gm decrease, but this relationship did not entirely explain the different genotypic gm responses. Moreover, the oxidative stress defence metabolites ascorbate and glutathione were not related to O3 tolerance of gm. However, malondialdehyde probably mitigated the observed gm decrease in some genotypes due to its oxidative stress signalling function. The large variation of gm suggests different regulation mechanisms amongst poplar genotypes under oxidative stress.
Assuntos
Ozônio , Populus , Dióxido de Carbono/metabolismo , Genótipo , Células do Mesofilo/fisiologia , Estresse Oxidativo , Ozônio/metabolismo , Ozônio/farmacologia , Fotossíntese , Folhas de Planta/metabolismo , Populus/metabolismoRESUMO
Ozone induces stomatal sluggishness, which impacts photosynthesis and transpiration. Stomatal responses to variation of environmental parameters are slowed and reduced by ozone and may be linked to difference of ozone sensitivity. Here we determine the ozone effects on stomatal conductance of each leaf surface. Potential causes of this sluggish movement, such as ultrastructural or ionic fluxes modification, were studied independently on both leaf surfaces of three Euramerican poplar genotypes differing in ozone sensitivity and in stomatal behaviour. The element contents in guard cells were linked to the gene expression of ion channels and transporters involved in stomatal movements, directly in microdissected stomata. In response to ozone, we found a decrease in the stomatal conductance of the leaf adaxial surface correlated with high calcium content in guard cells compared with a slight decrease on the abaxial surface. No ultrastructural modifications of stomata were shown except an increase in the number of mitochondria. The expression of vacuolar H(+) /Ca(2+) -antiports (CAX1 and CAX3 homologs), ß-carbonic anhydrases (ßCA1 and ßCA4) and proton H(+) -ATPase (AHA11) genes was strongly decreased under ozone treatment. The sensitive genotype characterized by constitutive slow stomatal response was also characterized by constitutive low expression of genes encoding vacuolar H(+) /Ca(2+) -antiports.
Assuntos
Ozônio/farmacologia , Estômatos de Plantas/anatomia & histologia , Estômatos de Plantas/fisiologia , Populus/genética , Populus/fisiologia , Elementos Químicos , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Genótipo , Microdissecção , Estômatos de Plantas/genética , Estômatos de Plantas/ultraestrutura , Populus/efeitos dos fármacosRESUMO
The occurrence of high-ozone concentrations during drought episodes is common considering that they are partially caused by the same meteorological phenomena. It was suggested that mild drought could protect plants against ozone-induced damage by causing the closure of stomata and preventing the entry of ozone into the leaves. The present experiment attempts to create an overview of the changes in cellular processes in response to ozone, mild drought and a combined treatment based on the use of 2D-DiGE to compare the involved proteins, and a number of supporting analyses. Morphological symptoms were worst in the combined treatment, indicating a severe stress, but fewer proteins were differentially abundant in the combined treatment than for ozone alone. Stomatal conductance was slightly lowered in the combined treatment. Shifts in carbon metabolism indicated that the metabolism changed to accommodate for protective measures and changes in the abundance of proteins involved in redox protection indicated the presence of an oxidative stress. This study allowed identifying a set of proteins that changed similarly during ozone and drought stress, indicative of crosstalk in the molecular response of plants exposed to these stresses. The abundance of other key proteins changed only when the plants are exposed to specific conditions. Together this indicates the coexistence of generalized and specialized responses to different conditions.
Assuntos
Ozônio/farmacologia , Folhas de Planta/metabolismo , Proteínas de Plantas/metabolismo , Populus/metabolismo , Proteoma/metabolismo , Adaptação Fisiológica , Análise por Conglomerados , Desidratação/metabolismo , Secas , Folhas de Planta/anatomia & histologia , Populus/anatomia & histologia , Proteômica , Estresse FisiológicoRESUMO
Oxidative stress caused by ozone (O3 ) affects plant development, but the roles of specific redox-homeostatic enzymes in O3 responses are still unclear. While growth day length may affect oxidative stress outcomes, the potential influence of day length context on equal-time exposures to O3 is not known. In Arabidopsisâ Col-0, day length affected the outcome of O3 exposure. In short-days (SD), few lesions were elicited by treatments that caused extensive lesions in long days (LD). Lesion formation was not associated with significant perturbation of glutathione, ascorbate, NADP(H) or NAD(H). To investigate roles of two genes potentially underpinning this redox stability, O3 responses of mutants for cytosolic NADP-isocitrate dehydrogenase (icdh) and glutathione reductase 1 (gr1) were analysed. Loss of ICDH function did not affect O3 -induced lesions, but slightly increased glutathione oxidation, induction of other cytosolic NADPH-producing enzymes and pathogenesis-related gene 1 (PR1). In gr1, O3 -triggered lesions, salicylic acid accumulation, and induction of PR1 were all decreased relative to Col-0 despite enhanced accumulation of glutathione. Thus, even at identical irradiance and equal-time exposures, day length strongly influences phenotypes triggered by oxidants of atmospheric origin, while in addition to its antioxidant function, the GR-glutathione system seems to play novel signalling roles during O3 exposure.
Assuntos
Proteínas de Arabidopsis/genética , Arabidopsis/enzimologia , Citosol/enzimologia , Glutationa Redutase/genética , Isocitrato Desidrogenase/genética , Mutação/genética , Ozônio/farmacologia , Fotoperíodo , Arabidopsis/efeitos dos fármacos , Arabidopsis/genética , Arabidopsis/fisiologia , Proteínas de Arabidopsis/metabolismo , Ácido Ascórbico/metabolismo , Citosol/efeitos dos fármacos , Ecótipo , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Técnicas de Inativação de Genes , Glutationa/metabolismo , Glutationa Redutase/metabolismo , Isocitrato Desidrogenase/metabolismo , NAD/metabolismo , NADP/metabolismo , Oxirredução/efeitos dos fármacos , Fenótipo , Fosfoenolpiruvato Carboxilase/metabolismo , Análise de Componente Principal , RNA Mensageiro/genética , RNA Mensageiro/metabolismoRESUMO
NADPH is a pivotal molecule in oxidative stress, during which it is potentially produced by several cytosolic NADP-linked dehydrogenases. This study investigated the response and functional importance of the major leaf cytosolic NADP-malic enzyme in Arabidopsis (NADP-ME2) during oxidative stress. Data from both microarray and targeted quantitative PCR analyses showed that NADP-ME2 transcripts accumulated in response to ozone or in mutants undergoing intracellular oxidative stress. To test the functional importance of this response, loss-of-function nadp-me2 mutants were obtained and the effects of oxidative stress of intracellular and extracellular origin were tested. Despite much decreased leaf NADP-ME activity, nadp-me2 showed a wild-type phenotype when exposed to ozone. Introduction of the nadp-me2 mutations into the catalase-deficient cat2 background did not alter growth inhibition or lesions triggered by intracellular oxidative stress. Similarly, loss of NADP-ME2 function had little effect on cat2-triggered changes in glutathione or NADPH. While single nadp-me2 mutations produced slight effects on basal resistance to one type of bacteria, they did not affect resistance induced by the cat2 mutation. Taken together, the results suggest that, although NADP-ME2 induction is part of the response to oxidative stress, the enzyme is not an essential determinant of the outcome of such stress.
Assuntos
Proteínas de Arabidopsis/genética , Arabidopsis/genética , Regulação da Expressão Gênica de Plantas , Malato Desidrogenase/genética , Estresse Oxidativo , Ozônio/toxicidade , Arabidopsis/enzimologia , Arabidopsis/microbiologia , Proteínas de Arabidopsis/metabolismo , Malato Desidrogenase/metabolismo , Mutação , Oxirredução , Folhas de Planta/enzimologia , Folhas de Planta/genética , Folhas de Planta/microbiologia , Pseudomonas syringae/genética , Pseudomonas syringae/fisiologiaRESUMO
Cell capacity for cytosolic NADPH regeneration by NADP-dehydrogenases was investigated in the leaves of two hybrid poplar (Populus deltoides × Populus nigra) genotypes in response to ozone (O3 ) treatment (120 ppb for 17 days). Two genotypes with differential O3 sensitivity were selected, based on visual symptoms and fallen leaves: Robusta (sensitive) and Carpaccio (tolerant). The estimated O3 flux (POD0 ), that entered the leaves, was similar for the two genotypes throughout the treatment. In response to that foliar O3 flux, CO2 assimilation was inhibited to the same extent for the two genotypes, which could be explained by a decrease in Rubisco (EC 4.1.1.39) activity. Conversely, an increase in PEPC (EC 4.1.1.31) activity was observed, together with the activation of certain cytosolic NADP-dehydrogenases above their constitutive level, i.e. NADP-G6PDH (EC 1.1.1.49), NADP-ME (malic enzyme) (EC 1.1.1.40) and NADP-ICDH (NADP-isocitrate dehydrogenase) (EC1.1.1.42). However, the activity of non-phosphorylating NADP-GAPDH (EC 1.2.1.9) remained unchanged. From the 11th fumigation day, NADP-G6PDH and NADP-ME profiles made it possible to differentiate between the two genotypes, with a higher activity in Carpaccio than in Robusta. At the same time, Carpaccio was able to maintain high levels of NADPH in the cells, while NADPH levels decreased in Robusta O3 -treated leaves. All these results support the hypothesis that the capacity for cells to regenerate the reducing power, especially the cytosolic NADPH pool, contributes to improve tolerance to high ozone exposure.
Assuntos
NADP/metabolismo , Ozônio/metabolismo , Populus/enzimologia , Dióxido de Carbono/metabolismo , Clorofila/metabolismo , Genótipo , NAD/metabolismo , Fosfoenolpiruvato Carboxilase/metabolismo , Populus/genética , Ribulose-Bifosfato Carboxilase/metabolismoRESUMO
After reaching phytotoxic levels during the last century, tropospheric ozone (O3) pollution is likely to remain a major concern in the coming decades. Despite similar injury processes, there is astounding interspecific-and sometimes intraspecific-foliar symptom variability, which may be related to spatial and temporal variation in injury dynamics. After characterizing the dynamics of physiological responses and O3 injury in the foliage of hybrid poplar in an earlier study, here we investigated the dynamics of changes in the cell structure occurring in the mesophyll as a function of O3 treatment, time, phytotoxic O3 dose (POD0), leaf developmental stage, and mesophyll layer. While the number of Hypersensitive Response-like (HR-like) lesions increased with higher O3 concentrations and POD0, especially in older leaves, most structural HR-like markers developed after cell death, independent of the experimental factors. The pace of degenerative Accelerated Cell Senescence (ACS) responses depended closely on the O3 concentration and POD0, in interaction with leaf age. Changes in total chlorophyll content, plastoglobuli and chloroplast shape pointed to thylakoid membranes in chloroplasts as being especially sensitive to O3 stress. Hence, our study demonstrates that early HR-like markers can provide reasonably specific, sensitive and reliable quantitative structural estimates of O3 stress for e.g. risk assessment studies, especially if they are associated with degenerative and thylakoid-related injury in chloroplasts from mesophyll.
Assuntos
Alcaloides , Ozônio , Populus , Toxinas Biológicas , Morte Celular , Senescência Celular , TilacoidesRESUMO
Populus tremula L. x P. alba L. (Populus x canescens (Aiton) Smith), clone INRA 717-1-B4, saplings were subjected to 120 ppb ozone exposure for 28 days. Chloroplasts were isolated, and the membrane proteins, solubilized using the detergent 1,2-diheptanoyl-sn-glycero-3-phosphocholine (DHPC), were analyzed in a difference gel electrophoresis (DiGE) experiment comparing control versus ozone-exposed plants. Extrinsic photosystem (PS) proteins and adenosine triphosphatase (ATPase) subunits were detected to vary in abundance. The general trend was a decrease in abundance, except for ferredoxin-NADP(+) oxidoreductase (FNR), which increased after the first 7 days of exposure. The up-regulation of FNR would increase NAPDH production for reducing power and detoxification inside and outside of the chloroplast. Later on, FNR and a number of PS and ATPase subunits decrease in abundance. This could be the result of oxidative processes on chloroplast proteins but could also be a way to down-regulate photochemical reactions in response to an inhibition in Calvin cycle activity.
Assuntos
Eletroforese em Gel Bidimensional/métodos , NADP/biossíntese , Ozônio/efeitos adversos , Fotossíntese/genética , Folhas de Planta/metabolismo , Proteínas de Plantas/metabolismo , Populus/metabolismo , Proteômica/métodos , Detergentes/química , Ferredoxina-NADP Redutase/genética , Ferredoxina-NADP Redutase/metabolismo , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Oxirredução/efeitos dos fármacos , Ozônio/farmacologia , Éteres Fosfolipídicos/química , Fotossíntese/efeitos dos fármacos , Folhas de Planta/genética , Proteínas de Plantas/genética , Populus/genética , Análise de Componente Principal , Transdução de Sinais/efeitos dos fármacos , Tilacoides/genética , Tilacoides/metabolismoRESUMO
Ozone is a secondary air pollutant, which causes oxidative stress in plants by producing reactive oxygen species (ROS) starting by an external attack of leaf apoplast. ROS have a dual role, acting as signaling molecules, regulating different physiological processes and response to stress, but also inducing oxidative damage. The production of ROS in plant cells is compartmented and regulated by scavengers and specific enzyme pathways. Chronic doses of ozone are known to trigger an important increase of the respiratory process while decreasing photosynthesis. Mitochondria, which normally operate with usual levels of intracellular ROS, would have to play a prominent role to cope with an enhanced ozone-derived ROS production. It is thus needed to compile the available literature on the effects of ozone on mitochondria to precise their strategy facing oxidative stress. An overview of the mitochondrial fate in three steps is proposed, i) starting with the initial responses of the mitochondria for alleviating the overproduction of ROS by the enhancement of existing antioxidant metabolism and adjustments of the electron transport chain, ii) followed by the setting up of detoxifying processes through exchanges between mitochondria and the cell, and iii) ending by an accelerated senescence initiated by mitochondrial membrane permeability and leading to programmed cell death.
Assuntos
Mitocôndrias , Estresse Oxidativo , Ozônio , Fenômenos Fisiológicos Vegetais , Antioxidantes/metabolismo , Mitocôndrias/metabolismo , Estresse Oxidativo/fisiologia , Ozônio/farmacologia , Espécies Reativas de Oxigênio/metabolismoRESUMO
With background concentrations having reached phytotoxic levels during the last century, tropospheric ozone (O3) has become a key climate change agent, counteracting carbon sequestration by forest ecosystems. One of the main knowledge gaps for implementing the recent O3 flux-based critical levels (CLs) concerns the assessment of effective O3 dose leading to adverse effects in plants. In this study, we investigate the dynamics of physiological, structural, and morphological responses induced by two levels of O3 exposure (80 and 100 ppb) in the foliage of hybrid poplar, as a function of phytotoxic O3 dose (POD0) and foliar developmental stage. After a latency period driven by foliar ontological development, the gas exchanges and chlorophyll content decreased with higher POD0 monotonically. Hypersensitive response-like lesions appeared early during exposure and showed sigmoidal-like dynamics, varying according to leaf age. At current POD1_SPEC CL, notwithstanding the aforementioned reactions and initial visible injury to foliage, the treated poplars had still not shown any growth or biomass reduction. Hence, this study demonstrates the development of a complex syndrome of early reactions below the flux-based CL, with response dynamics closely determined by the foliar ontological stage and environmental conditions. General agreement with patterns observed in the field appears indicative of early O3 impacts on processes relevant, e.g., biodiversity ecosystem services before those of economic significance - i.e., wood production, as targeted by flux-based CL.
RESUMO
Tropospheric ozone is the main atmospheric pollutant that causes damages to trees. The estimation of the threshold for ozone risk assessment depends on the evaluation of the means that this pollutant impacts the plant and, especially, the foliar organs. The available results show that, before any visible symptom appears, carbon assimilation and the underlying metabolic processes are decreased under chronic ozone exposure. By contrast, the catabolic pathways are enhanced, and contribute to the supply of sufficient reducing power necessary to feed the detoxification processes. Reactive oxygen species delivered during ozone exposure serve as toxic compounds and messengers for the signaling system. In this review, we show that the contribution of genomic tools (transcriptomics, proteomics, and metabolomics) for a better understanding of the mechanistic cellular responses to ozone largely relies on spectrometric measurements.
Assuntos
Ozônio/metabolismo , Plantas/genética , Plantas/metabolismo , Populus/metabolismo , Poluentes Atmosféricos/metabolismo , Regulação da Expressão Gênica de Plantas , Metabolômica/métodos , Oxirredução , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Populus/genética , Proteômica/métodosRESUMO
Populus tremula L. × Populus alba L. (Populus ×c anescens (Aiton) Smith) - clone INRA 717-1-B4 saplings (50 cm apex to base and carrying 19 leaves on average) - were followed for 28 days. Half of the trees were grown in charcoal-filtered air while the other half were exposed to 120 ppb ozone for 11 h a day during the light period. The expanding leaf number 4 was tagged at the beginning of the experiment and finished expansion between 7 and 14 days. These leaves were harvested weekly for biochemical and proteome analyses using quantitative bidimensional electrophoresis (DiGE). Independent of the ozone treatment, all the analyses allowed a distinction between expanding and adult leaves. The results indicate that during the expansion phase (Days 0-7) the enzymatic machinery of the leaves is set up, and remains dynamically stable in the adult leaves (Days 14-28). Although ozone had no apparent effect on expanding leaves, the metabolic stability in fully expanded leaves observed in ozone-free plants was disturbed after 2 weeks of exposure and a stress-induced response became apparent.
Assuntos
Poluentes Atmosféricos/toxicidade , Ecossistema , Ozônio/efeitos adversos , Folhas de Planta/efeitos dos fármacos , Populus/efeitos dos fármacos , Plântula/efeitos dos fármacos , Clorofila/metabolismo , Eletroforese , Fotossíntese/efeitos dos fármacos , Complexo de Proteínas do Centro de Reação Fotossintética/efeitos dos fármacos , Complexo de Proteínas do Centro de Reação Fotossintética/metabolismo , Folhas de Planta/enzimologia , Folhas de Planta/crescimento & desenvolvimento , Proteínas de Plantas/metabolismo , Populus/enzimologia , Populus/crescimento & desenvolvimento , Proteômica/métodos , Plântula/enzimologia , Plântula/crescimento & desenvolvimento , Estresse Fisiológico/efeitos dos fármacos , Fatores de TempoRESUMO
Tropospheric ozone (O3) is the main secondary pollutant and considered to be the most damaging for growth and productivity. O3 is well known to induce oxidative stress and Reactive Oxygen Species accumulation in leaf tissues. Several mechanisms have been suggested to enable trees to cope with such stress; however, their relative contribution to O3 tolerance is still unclear. Here, ten Euramerican poplar genotypes (Populus deltoidesâ¯×â¯nigra) were investigated regarding their response to 120â¯ppb of O3 for 3â¯weeks in order to determine main mechanisms and identify the key traits and strategies linked to a better tolerance to O3-induced oxidative stress. Results showed that ascorbate peroxidase and ascorbate regeneration through monodehydroascorbate reductase are the main determinants of O3 tolerance in Euramerican poplar, in protecting photosynthesis capacity from oxidative stress and therefore, maintaining growth and productivity. Besides, stomatal closure was harmful in sensitive genotypes, suggesting that avoiding strategy can be further deleterious under chronic ozone. Finally, O3-induced early senescence appeared essential when up scaling leaf-level mechanistic response to whole-plant productivity, in fine-tuning resource reallocation and photosynthesis area.
Assuntos
Poluentes Atmosféricos/metabolismo , Genótipo , Estresse Oxidativo , Ozônio/metabolismo , Populus/fisiologia , Fotossíntese , Folhas de Planta/fisiologia , Populus/genéticaRESUMO
Stresses such as wounding or atmospheric pollutant exposure have a significant impact on plant fitness. Since it has been widely described that the metabolome directly reflects plant physiological status, a way to assess this impact is to perform a global metabolomic analysis. In this study, we investigated the effect of two abiotic stresses (mechanical wounding and ozone exposure) on parsnip metabolic balance using a liquid chromatography-mass spectrometry-based untargeted metabolomic approach. For this purpose, parsnip leaves were submitted to an acute ozone exposure or were mechanically wounded and sampled 24, 48, and 72 h post-treatment. Multivariate and univariate statistical analyses highlighted numerous differentially-accumulated metabolic features as a function of time and treatment. Mechanical wounding led to a more differentiated response than ozone exposure. We found that the levels of coumarins and fatty acyls increased in wounded leaves, while flavonoid concentration decreased in the same conditions. These results provide an overview of metabolic destabilization through differentially-accumulated compounds and provide a better understanding of global plant metabolic changes in defense mechanisms.
RESUMO
Ozone (O3) and drought increase tree oxidative stress. To protect forest health, we need to improve risk assessment, using metric model such as the phytotoxic O3 dose above a threshold of yâ¯nmol·m-2·s-1 (PODy), while taking into account detoxification mechanisms and interacting stresses. The impact of drought events on the effect of O3 pollution deserves special attention. Water deficit may decrease O3 entrance into the leaves by reducing stomatal opening; however, water deficit also induces changes in cell redox homeostasis. Besides, the behaviour of the cell antioxidative charge in case of stress combination (water deficit and O3) still remains poorly investigated. To decipher the response of detoxification mechanisms relatively to the Halliwell-Asada-Foyer cycle (HAF), we exposed poplar saplings (Populus nigraâ¯×â¯deltoides) composed of two genotypes (Carpaccio and Robusta), to various treatments for 17â¯days, i.e. i) mild water deficit, ii) 120â¯ppb O3, and iii) a combination of these two treatments. Ozone similarly impacted the growth of the two genotypes, with an important leaf loss. Water deficit decreased growth by almost one third as compared to the control plants. As for the combined treatment, water deficit protected the saplings from leaf ozone injury, but with an inhibitory effect on growth. The pool of total ascorbate was not modified by the different treatments, while the pool of total glutathione increased with POD0. We noticed a few differences between the two genotypes, particularly concerning the activity of monodehydroascorbate reductase and glutathione reductase relatively to POD0. The expression profiles of genes coding for the dehydroascorbate reductase and glutathione reductase isoforms differed, probably in link with the putative localisation of ROS production in response to water deficit and ozone, respectively. Our result would argue for a major role of MDHAR, GR and glutathione in the preservation of the redox status.
Assuntos
Ácido Ascórbico/metabolismo , Secas , Ozônio/efeitos adversos , Populus/metabolismo , Expressão Gênica/efeitos dos fármacos , Genótipo , Glutationa/metabolismo , Inativação Metabólica , Estresse Oxidativo , Populus/enzimologia , Populus/genética , Água/metabolismoRESUMO
The impact of ozone (O3) pollution events on the plant drought response needs special attention because spring O3 episodes are often followed by summer drought. By causing stomatal sluggishness, O3 could affect the stomatal dynamic during a subsequent drought event. In this context, we studied the impact of O3 exposure and water deficit (in the presence or in the absence of O3 episode) on the stomatal closure/opening mechanisms relative to irradiance or vapour pressure deficit (VPD) variation. Two genotypes of Populus nigra x deltoides were exposed to various treatments for 21 days. Saplings were exposed to 80â¯ppb/day O3 for 13 days, and then to moderate drought for 7 days. The curves of the stomatal response to irradiance and VPD changes were determined after 13 days of O3 exposure, and after 21 days in the case of subsequent water deficit, and then fitted using a sigmoidal model. The main responses under O3 exposure were stomatal closure and sluggishness, but the two genotypes showed contrasting responses. During stomatal closure induced by a change in irradiance, closure was slower for both genotypes. Nonetheless, the genotypes differed in stomatal opening under light. Carpaccio stomata opened more slowly than control stomata, whereas Robusta stomata tended to open faster. These effects could be of particular interest, as stomatal impairment was still present after O3 exposure and could result from imperfect recovery. Under water deficit alone, we observed slower stomatal closure in response to VPD and irradiance, but faster stomatal opening in response to irradiance, more marked in Carpaccio. Under the combined treatment, most of the parameters showed antagonistic responses. Our results highlight that it is important to take genotype-specific responses and interactive stress cross-talk into account to improve the prediction of stomatal conductance in response to various environmental modifications.
Assuntos
Poluentes Atmosféricos/toxicidade , Ozônio/toxicidade , Estômatos de Plantas/efeitos dos fármacos , Populus/efeitos dos fármacos , Água/metabolismo , Secas , Genótipo , Modelos Teóricos , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/genética , Estômatos de Plantas/genética , Populus/genética , Estações do Ano , Especificidade da Espécie , Pressão de VaporRESUMO
Plants are frequently exposed to adverse environmental conditions such as drought and ozone (O3). Under these conditions, plants can survive due to their ability to adjust their metabolism. The aim of the present study was to compare the detoxification mechanisms of three oak species showing different O3 sensitivity and water use strategy. Two-year-old seedlings of Quercus ilex, Q. pubescens and Q. robur were grown under the combination of three levels of O3 (1.0, 1.2 and 1.4 times the ambient O3 concentration) and three levels of water availability (on average 100, 80 and 42% of field capacity i.e. well-watered, moderate drought and severe drought, respectively) in an O3 Free Air Controlled Exposure facility. Ozone and drought induced the accumulation of reactive oxygen species (ROS) and this phenomenon was species-specific. Sometimes, ROS accumulation was not associated with membrane injury suggesting that several antioxidative defence mechanisms inhibited or alleviated the oxidative damage. Both O3 and drought increased total carotenoids that were able to prevent the peroxidation action by free radicals in Q. ilex, as confirmed by unchanged malondialdehyde by-product values. The concomitant decrease of total flavonoids may be related to the consumption of these compounds by the cell to inhibit the accumulation of hydrogen peroxide. Unchanged total phenols confirmed that Q. ilex has a superior ability to counteract oxidative conditions. Similar responses were found in Q. pubescens, although the negative impact of both factors was less efficiently faced than in the sympatric Q. ilex. In Q. robur, high O3 concentrations and severe drought induced a partial rearrangement of the phenylpropanoid pathways. These antioxidative mechanisms were not able to protect the cell structure (as confirmed by ROS accumulation) suggesting that Q. robur showed a lower degree of tolerance than the other two species.
Assuntos
Poluentes Atmosféricos/toxicidade , Antioxidantes/metabolismo , Ozônio/toxicidade , Quercus/fisiologia , Secas , Folhas de Planta , Estresse FisiológicoRESUMO
Young poplar trees (Populus tremula Michx. x Populus alba L. clone INRA 717-1B4) were subjected to 120 ppb of ozone for 35 days in phytotronic chambers. Treated trees displayed precocious leaf senescence and visible symptoms of injury (dark brown/black upper surface stippling) exclusively observed on fully expanded leaves. In these leaves, ozone reduced parameters related to photochemistry (Chl content and maximum rate of photosynthetic electron transport) and photosynthetic CO(2) fixation [net CO(2) assimilation, Rubisco (ribulose-1,5-bisphosphate carboxylase oxygenase) activity and maximum velocity of Rubisco for carboxylation]. In fully expanded leaves, the rate of photorespiration as estimated from Chl fluorescence was markedly impaired by the ozone treatment together with the activity of photorespiratory enzymes (Rubisco and glycolate oxidase). Immunoblot analysis revealed a decrease in the content of serine hydroxymethyltransferase in treated mature leaves, while the content of the H subunit of the glycine decarboxylase complex was not modified. Leaves in the early period of expansion were exempt from visible symptoms of injury and remained unaffected as regards all measured parameters. Leaves reaching full expansion under ozone exposure showed potential responses of protection (stimulation of mitochondrial respiration and transitory stomatal closure). Our data underline the major role of leaf phenology in ozone sensitivity of photosynthetic processes and reveal a marked ozone-induced inhibition of photorespiration.