RESUMO
Noroviruses, a major cause of acute gastroenteritis outbreaks worldwide, are constantly evolving. This ability is reflected in the speed and efficiency with which these viruses spread and remain in the human population. The present study reports the detection of a novel recombination event among norovirus genotypes in Brazil in 2008. A strain detected in a stool sample from a child with norovirus-associated gastroenteritis, residing in an African-descendant semi-closed community of Pará State, was characterized as a novel intergenotype recombinant, GII.7/GII.20, as determined by partial sequencing and SimPlot analysis.
Assuntos
População Negra , Infecções por Caliciviridae/virologia , Gastroenterite/virologia , Norovirus/classificação , Norovirus/genética , Vírus Reordenados , Sequência de Bases , Brasil/epidemiologia , Infecções por Caliciviridae/epidemiologia , Fezes/virologia , Gastroenterite/epidemiologia , Humanos , Lactente , Dados de Sequência Molecular , FilogeniaRESUMO
Using cDNA microarray assays we have observed a clear difference in the gene expression pattern between bone marrow stromal cells obtained from healthy children (CT) and from pediatric patients with either myelodysplastic syndromes (MDS) or acute myeloid leukemia (AML) associated with MDS (MDS-AML). The global gene function profiling analysis indicated that in the pediatric MDS microenvironment the disease stages may be characterized mainly by underexpression of genes associated with biological processes such as transport. Furthermore, a subset of downregulated genes related to endocytosis and protein secretion was able to discriminate MDS from MDS-AML.