Characteristics of Staphylococcus aureus biofilm matured in tryptic soy broth, low-fat milk, or whole milk samples along with inactivation by 405 nm light combined with folic acid.

In the present study, the characteristics of Staphylococcus aureus biofilms matured in tryptic soy broth (TSB), low-fat milk, or whole milk samples were identified along with their resistance to 405 nm light with or without folic acid. Phenotypic properties of carbohydrate and protein contents in extracellular polymeric substance (EPS) of S. aureus biofilms matured in different conditions were identified. The carbohydrate content was higher in the biofilm matured in low-fat milk (1.27) than the samples matured in whole milk (0.58) and TSB (0.10). Protein content in the EPS of biofilm was higher in the sample matured in whole milk (6.59) than the samples matured in low-fat milk (3.24) and TSB (2.08). Moreover, the maturation condition had a significant effect on the membrane lipid composition of the biofilm, producing more unsaturated fatty acids in biofilm matured in milk samples. These changes in biofilm matured in milk samples increased the resistance of S. aureus to 405 nm light in the presence of folic acid (LFA). Additionally, transcriptomic analysis was conducted to identify the response of S. aureus biofilm to LFA treatment. Several genes related to DNA and protein protection from oxidative stress along with biofilm accumulation were overexpressed in the LFA-treated biofilms. These results indicate the maturation of S. aureus biofilm in various samples and the biofilms responses to bactericidal treatments.

Inactivation Kinetics and Membrane Potential of Pathogens in Soybean Curd Subjected to Pulsed Ohmic Heating Depending on Applied Voltage and Duty Ratio.

The aim of this research was to investigate the efficacy of the duty ratio and applied voltage in the inactivation of pathogens in soybean curd by pulsed ohmic heating (POH). The heating rate of soybean curd increased rapidly as the applied voltage increased, although the duty ratio did not affect the temperature profile. We supported this result by verifying that electrical conductivity increased with the applied voltage. Escherichia coli O157:H7, Salmonella enterica serovar Typhimurium, and Listeria monocytogenes in soybean curd were significantly (P < 0.05) inactivated by more than 1 log unit at 80 Vrms (root mean square voltage). To elucidate the mechanism underlying these results, the membrane potential of the pathogens was examined using DiBAC4(3) [bis-(1,3-dibutylbarbituric acid)trimethine oxonol] on the basis of a previous study showing that the electric field generated by ohmic heating affected the membrane potential of cells. The values of DiBAC4(3) accumulation increased under increasing applied voltage, and they were significantly (P < 0.05) higher at 80 Vrms, while the duty ratio had no effect. In addition, morphological analysis via transmission electron microscopy showed that electroporation and expulsion of intracellular materials were predominant at 80 Vrms Moreover, electrode corrosion was overcome by the POH technique, and the textural and color properties of soybean curd were preserved. These results substantiate the idea that the applied voltage has a profound effect on the microbial inactivation of POH as a consequence of not only the thermal effect, but also the nonthermal effect, of the electric field, whereas the duty ratio does not have such an effect.IMPORTANCE High-water-activity food products, such as soybean curd, are vulnerable to microbial contamination, which causes fatal foodborne diseases and food spoilage. Inactivating microorganisms inside food is difficult because the transfer of thermal energy is slower inside than it is outside the food. POH is an adequate sterilization technique because of its rapid and uniform heating without causing electrode corrosion. To elucidate the electrical factors associated with POH performance in the inactivation of pathogens, the effects of the applied voltage and duty ratio on POH were investigated. In this study, we verified that a high applied voltage (80 Vrms) at a duty ratio of 0.1 caused thermal and nonthermal effects on pathogens that led to an approximately 4-log-unit reduction in a significantly short time. Therefore, the results of this research corroborate database predictions of the inactivation efficiency of POH based on pathogen control strategy modeling.

Application of low frequency pulsed ohmic heating for inactivation of foodborne pathogens and MS-2 phage in buffered peptone water and tomato juice.

The purpose of this study was to inactivate foodborne pathogens effectively by ohmic heating in buffered peptone water and tomato juice without causing electrode corrosion and quality degradation. Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes were used as representative foodborne pathogens and MS-2 phage was used as a norovirus surrogate. Buffered peptone water and tomato juice inoculated with pathogens were treated with pulsed ohmic heating at different frequencies (0.06-1 kHz). Propidium iodide uptake values of bacterial pathogens were significantly (p < 0.05) larger at 0.06-0.5 kHz than at 1 kHz, and sub-lethal injury of pathogenic bacteria was reduced by decreasing frequency. MS-2 phage was inactivated more effectively at low frequency, and was more sensitive to acidic conditions than pathogenic bacteria. Electrode corrosion and quality degradation of tomato juice were not observed regardless of frequency. This study suggests that low frequency pulsed ohmic heating is applicable to inactivate foodborne pathogens effectively without causing electrode corrosion and quality degradation in tomato juice.

Inactivation of Cronobacter sakazakii in powdered infant formula with probiotics and metagenomic analysis.

In the present study, proper manual for powdered infant formula with probiotics (PIF-P) to prevent the contamination of Cronobacter sakazakii was investigated. First, the population of C. sakazakii and LAB in three different PIF-P samples were quantitatively analyzed after reconstituted with hydrothermal treatments. When C. sakazakii was inoculated into reconstituted infant formula with probiotics (RIF-P), it was immediately reduced below the detection limit by 60-65 °C hydrothermal treatment whereas reduction levels of LAB was 1-2 log CFU/g. When heat resistance of C. sakazakii inoculated to PIF-P with 4 h drying was compared with that inoculated to RIF-P samples, the heat resistance of C. sakazakii increased significantly after the inoculation in PIF-P with drying. Metagenomic analysis revealed that Lactobacillus and Bifidobacterium were dominant genus in all three groups and there was no significant difference in the microbial community of untreated PIF sample and hydrothermal treated samples. Supplementary Information: The online version contains supplementary material available at 10.1007/s10068-023-01503-x.

Impact and prospect of the fourth industrial revolution in food safety: Mini-review.

The fourth industrial revolution represented by big data and artificial intelligence (AI), already had a significant impact on the food industry. In this review, the impacts and prospects of the 4th industrial revolution in food safety were discussed. First, the general process and characteristics of AI application from data collection to visualization are covered. Additionally, various data collection and analysis methods are discussed, with emphasis on the collection of high variety, volume, and velocity data and visualization. Available literature presents examples of machine learning applications in food samples that are mostly associated with the classification of agricultural food items through convolutional neural networks. Based on these examples, the prospects of the 4th industrial revolution in food safety are categorized as follows: prediction of food safety risk, detection of foodborne pathogens, and food safety management. This mini-review will help understand the relationship between the 4th industrial revolution and food safety.

The combined effect of folic acid and 365-405 nm light emitting diode for inactivation of foodborne pathogens and its bactericidal mechanisms.

The inactivation of Escherichia coli O157:H7 ATCC 35150 and methicillin-resistant S. aureus ATCC 33591 by a light emitting diode (LED) was investigated in the present study. Various wavelengths of LEDs (365, 385, and 405 nm) were used individually or combined with folic acid (LEDF), and inactivation curves were analyzed using the Weibull model to compare the bactericidal effects. Reduction levels of pathogens by combination treatment of LED and 100 µM folic acid were significantly higher than those by individual LED treatment for the wavelength used in this study. It was confirmed that the reactive oxygen species produced by folic acid degradation accelerated the bactericidal effect of LEDs. When applied in apple juice, the same trend was observed by 405 nm treatment, although the inactivation rates were higher than those of buffer due to the low pH of apple juice. Moreover, injured cells were not observed except 30 J/cm2 LED, LEDF and 36 J/cm2 LEDF treatments. Mode of inactivation by LEDF was suggested as DNA damage along with membrane damages. Moreover, it was validated that genes related to antimicrobial resistance would be mutated by LEDF treatment. Therefore, LEDF can be used effectively to control foodborne pathogens in apple juice.

Stress response of Salmonella Montevideo adapted to red pepper powders at various humidities and resistance to near-infrared heating.

In the present study, the stress responses of Salmonella Montevideo adapted to red pepper powder with various humidities were investigated. The resistance of S. Montevideo to near-infrared heating increased significantly when adapted to red pepper powder. Moreover, the pathogen turned to a viable but noncultureable (VBNC) state after storage in red pepper powder at 30 °C with 40 % relative humidity (RH). Therefore, transcriptomic analysis was conducted for pathogens adapted to red pepper powder at 30 °C and 40 % RH for 4 h (VBNC transformation starting point). Compared to the transcriptome from the pathogen grown in tryptic soy broth at 37 °C, a total of 1,268 differentially expressed genes (DEGs) were observed with the criteria of ΙlogFCΙ ≥ 2 and false discovery rate ≤ 0.05, including 229 upregulated and 1,039 downregulated genes. It was confirmed that heat shock protein-related genes such as dnaK and dnaJ were highly overexpressed in the pathogen adapted to red pepper powder. Moreover, genes contributing to the invasion and internalization of S. Montevideo to red pepper powder were upregulated. Finally, higher gene expression levels of the type III secretion system (ssaS, ssaL, sseG, and pipB) with lower sample humidity were observed with quantitative real-time PCR. These results indicated that genomic changes contribute to phenotypic changes, such as heat resistance changes and transformation to the VBNC state.

Inactivation of Salmonella enterica Serovar Typhimurium and Staphylococcus aureus in Rice by Radio Frequency Heating.

ABSTRACT: The objectives of this study were to determine the effect of the milling degree (MD) of rice (Oryza sativa L.) on the heating rate, pathogen inactivation (Salmonella Typhimurium and Staphylococcus aureus), and color change resulting from radio frequency (RF) heating. Rice samples inoculated with pathogens were placed in a polypropylene jar and subjected to RF heating for 0 to 75 s. The heating rate of rice with a 2% MD was the highest during RF heating, followed by those with a 0, 8, and 10% MD; the reduction of pathogens showed the same trend. The reductions of pathogen levels in rice with MDs of 0 and 2% were significantly higher than those observed for rice with MDs of 8 and 10% under the same treatment conditions. For example, log reductions of Salmonella Typhimurium in rice by 55-s RF heating were 3.64, 5.19, 2.18, and 1.80 for MDs of 0, 2, 8, and 10%, respectively. At the same treatment conditions, log reductions of S. aureus were 2.77, 5.08, 1.15, and 0.90 for MDs of 0, 2, 8, and 10%, respectively. The color of rice measured according to L*, a*, and b* was not significantly altered after RF heating, regardless of the MD. Therefore, the MD of rice should be considered before RF heating is applied to inactivate foodborne pathogens.

Real-time analysis and predictability of the health functional food market using big data.

This study conducted a real-time analysis of the health functional food market using big data. To assess the scope of big data in market analysis, big data of the health food category were compared and analyzed with actual market data. Data were first collected using a program to obtain data, through application programming interfaces, followed by SPSS to compare and analyze the actual market index and shopping search word data. The correlation between the online search data and the actual market was high, indicating that online search data can be used to predict the trend of the actual market. Various types of data, such as items and major functional ingredients, can be collected and analyzed through the program developed for this study, which is also used to predict the market trend. The results demonstrate how APIs can be used to predict market size in the food industry effectively.

Recent (2011-2017) foodborne outbreak cases in the Republic of Korea compared to the United States: a review.

This study analyzes and compares foodborne disease outbreaks reported in the Republic of Korea (KR) and the United States (US) during 2011-2017. The foodborne outbreaks data in the KR and the US were collected from the Ministry of Food and Drug Safety and from the Surveillance for Foodborne Disease Outbreaks United States, respectively. The average number of outbreaks and illness population were higher in the US than in the KR, but the KR's illness ratio considering population size was 2.4 times higher than that of the US. When the sites of outbreaks compared, the number of illness was the highest at schools in the KR whereas outbreaks at restaurants were more frequent in the US. In the KR, bacterial infections were the primary cause of outbreaks while bacterial and viral infections accounted for the largest share of outbreaks in the US. Specifically, pathogenic E. coli presented a significant risk in the KR whereas Salmonella was the most prevalent in the US. These results indicate that the main microbiological targets for detection and control in the KR should differ from the US, which should be considered for developing food safety related policies.

Development of sustained release formulations of chlorine dioxide gas for inactivation of foodborne pathogens on produce.

Formulations for the sustained release of chlorine dioxide (ClO2) gas were developed, and their gas-producing profiles and antimicrobial effects against Escherichia coli O157:H7 and Salmonella Typhimurium were evaluated in spinach leaves and tomatoes under different relative humidity (RH) conditions. Sodium chlorite (NaClO2) and citric acid were used to generate ClO2 gas, and the generation rate and maximum ClO2 gas concentration were controlled using diatomaceous earth (DE) and calcium chloride (CaCl2). Under 90% RH conditions, sustained release of ClO2 gas was achieved in presence of DE. When 12 g of DE was added to the mixture, the ClO2 gas concentration remained constant at 18 ± 1 ppmv for approximately 28 h. At 50% RH, addition of CaCl2 was effective in maintaining a constant ClO2 gas concentration. When 0.05 g of CaCl2 was added to mixtures containing 0.5 g of DE, ClO2 gas concentration remained constant at 11 ± 1 ppmv for approximately 26 h. Treatment with 30 ppmv of ClO2 gas at 90% RH achieved more than 6.16 and 5.48 log reductions of E. coli O157:H7 and S. Typhimurium on spinach leaves (in 15 min), and more than 6.78 and 6.34 log reductions of the same in tomatoes (in 10 min). The sustained release formulations for ClO2 gas, developed in this study, could facilitate the use of ClO2 gas as an antimicrobial agent in the food industry.

Outbreaks, Germination, and Inactivation of Bacillus cereus in Food Products: A Review.

ABSTRACT: Bacillus cereus has been reported as a foodborne pathogen worldwide. Although food processing technologies to inactivate the pathogen have been developed for decades, foodborne outbreaks related to B. cereus have occurred. In the present review, foodborne outbreaks, germination, inactivation, and detection of B. cereus are discussed, along with inactivation mechanisms. B. cereus outbreaks from 2003 to 2016 are reported based on food commodity, number of cases, and consequent illnesses. Germination before sporicidal treatments is highlighted as an effective way to inactivate B. cereus, because the resistance of the pathogen increases significantly following sporulation. Several germinants used for B. cereus are listed, and their efficacies are compared. Finally, recently used interventions with sporicidal mechanisms are identified, and rapid detection methods that have been developed are discussed. Combining two or more interventions, known as the hurdle technology concept, is suggested to maximize the sporicidal effect. Further study is needed to ensure food safety and to understand germination mechanisms and sporicidal resistance of B. cereus.

Antibacterial and in vitro antidementia effects of aronia (Aronia melanocarpa) leaf extracts.

This study investigated the antibacterial and in vitro antidementia effects of aronia (Aronia melanocarpa) leaf extracts from 3 cultivars (Nero, Viking, and McKenzie) collected at three different stages of maturity (young, harvest, and old). Bacillus cereus was susceptible to the old leaves of cultivars McKenzie and Nero, whereas Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria innocua were not inhibited by any of the extracts. Growth of B. cereus was inhibited by cv. McKenzie, resulting in increased lag time, whereas Nero had both an inhibitory and an inactivation effect. Except for cv. Viking at harvest stage, the acetylcholinesterase and butyrylcholinesterase inhibitory activity of aronia leaf extracts were about 60-70 and 70-80%, respectively. Therefore, aronia leaf is a natural resource with a potentially potent antidementia effect, besides antibacterial activity.

Inactivation of Bacillus cereus Spores on Stainless Steel by Combined Superheated Steam and UV-C Irradiation Treatment.

Bacillus cereus spore contamination on food contact surfaces is of great concern in the food industry. Thus, in the present study, superheated steam (SHS) was used alone or combined with UV-C irradiation for inactivation of B. cereus spores inoculated on stainless steel coupons. Temperatures higher than 250°C were needed to effectively inactivate B. cereus spores by SHS treatment alone, while a synergistic bactericidal effect resulted from the sequential treatment of SHS before or after UV-C irradiation. The increased dipicolinic acid ratio obtained by the combined treatment had a significant role in the synergistic bactericidal effect. Therefore, the combined treatment of SHS and UV-C could be used effectively to inactivate B. cereus on stainless steel. It is recommended to use hurdle technology with reduced energy consumption to ensure microbiological safety on food contact surfaces.

Numerical analysis of rectangular type batch ohmic heater to identify the cold point.

The objective of this research was to precisely simulate the temperature distribution and inactivation of Escherichia coli O157:H7 by batch ohmic heating pasteurization of orange juice based on a time-dependent numerical model. A finite element method (FEM) embedded with pathogen inactivation codes using Java language simultaneously solved electric heating, k-ε turbulent flow, and heat transfer equations and dealt with natural heat losses through the walls and air as the boundary conditions. The simulated temperature distribution and populations of E. coli O157:H7 did not differ from the experimental data for every treatment time within a relative error of 6.0%. A cold point problem was observed in the bottom corner, which was more severe for large orange juice samples, leading to an increased treatment time in order to ensure a 5-log reduction of E. coli O157:H7.

Reactive-oxygen-species-mediated mechanism for photoinduced antibacterial and antiviral activities of Ag3PO4.

Cubic-shaped Ag3PO4 crystals with a mean size of 1 µm were synthesized by a precipitation method from a mixed solution of AgNO3, Na2HPO4, and triethanolamine. The antibacterial activities against Escherichia coli, Listeria innocua, and Pseudomonas syringae DC3000 in both the absence and presence of Ag3PO4 under dark conditions and in the presence of Ag3PO4 under red-light (625 nm) and blue-light (460 nm) irradiation were examined. The concentrations of reactive oxygen species (ROS) were also measured in the antibacterial action of the Ag3PO4 against Escherichia coli. The photoinduced enhancement of the Ag3PO4 antibacterial activity under blue-light irradiation is explained by the formation of ROS during the antibacterial action of the Ag3PO4. Moreover, the antiviral activity of Ag3PO4 against amphotropic 10A1 murine leukemia virus enhanced under blue-light irradiation via ROS production. These results provide an insight into extended bio-applications of Ag3PO4.

Susceptibility of Escherichia coli O157:H7 grown at low temperatures to the krypton-chlorine excilamp.

This study was conducted to investigate the resistance of Escherichia coli O157:H7 to 222-nm krypton-chlorine(KrCl) excilamp and 254-nm low-pressure Hg lamp (LP lamp) treatment according to growth temperature. As growth temperature decreased, lag time of E. coli O157:H7 significantly increased while the growth rate significantly decreased. Regardless of growth temperature, the KrCl excilamp showed higher disinfection capacity compared to the LP lamp at stationary growth phase. KrCl excilamp treatment showed significantly higher reduction as growth temperature decreased. Conversely, reduction levels according to growth temperature were not significantly different when the pathogen was subjected to LP lamp treatment. Inactivation mechanisms were evaluated by the thiobarbituric acid reactive substances (TBARS) assay and SYBR green assay, and we confirmed that lipid oxdiation capacity following KrCl excilamp treatment increased as growth temperature decreased, which was significantly higher than that of LP lamp treated samples regardless of growth temperature. DNA damage level was significantly higher for LP Hg lamp treated samples compared to those subjected to the KrCl excilamp, but no significant difference pursuant to growth temperature was observed. At the transcriptional level, gene expression related to several metabolic pathways was significantly higher for the pathogen grown at 15 °C compared that of 37 °C, enabling it to adapt and survive at low temperature, and membrane lipid composition became altered to ensure membrane fluidity. Consequently, resistance of E. coli O157:H7 to the KrCl excilamp decreased as growth temperature decreased because the ratio of unsaturated fatty acid composition increased at low growth temperature resulting in higher lipid oxidation levels. These results indicate that KrCl excilamp treatment should be determined carefully considering the growth temperature of E. coli O157:H7.

Inactivation of Staphylococcus aureus Biofilms on Food Contact Surfaces by Superheated Steam Treatment.

The objective of this study was to compare the inactivation efficacy of saturated steam (SS) and superheated steam (SHS) on Staphylococcus aureus biofilms on food contact surfaces, including type 304 stainless steel coupons with No. 4 finish (STS No. 4), type 304 stainless steel coupons with 2B finish (STS 2B), high-density polyethylene (HDPE), and polypropylene (PP). In addition, the effects of the surface characteristics on the inactivation efficacy were evaluated. Biofilms were formed on each food contact coupon surface using a three-strain cocktail of S. aureus. Five-day-old biofilms on STS No. 4, STS 2B, HDPE, and PP coupons were treated with SS at 100°C and SHS at 125 and 150°C for 2, 4, 7, 10, 15, and 20 s. Among all coupon types, SHS was more effective than SS in inactivating the S. aureus biofilms. S. aureus biofilms on steel coupons were more susceptible to most SS and SHS treatments than the biofilms on plastic coupons. S. aureus biofilms on HDPE and PP coupons were reduced by 4.00 and 5.22 log CFU per coupon, respectively, after SS treatment (100°C) for 20 s. SS treatment for 20 s reduced the amount of S. aureus biofilm on STS No. 4 and STS 2B coupons to below the detection limit. With SHS treatment (150°C), S. aureus biofilms on HDPE and PP needed 15 s to be inactivated to below the detection limit, while steel coupons only needed 10 s. The results of this study suggest that SHS treatment has potential as a biofilm control intervention for the food industry.

Inactivation of Bacillus cereus ATCC 14579 Spore on Garlic with Combination Treatments of Germinant Compounds and Superheated Steam.

HIGHLIGHTS: Germinant compounds were combined with SHS to inactivate B. cereus spores. B. cereus spores were inactivated effectively by SHS after Ala+IMP treatment. Inactivation trend was analyzed by the Weibull model, and t3 d values were determined. Sublethal injury was not observed by SHS after Ala+IMP treatment.

Inactivation dynamics of 222 nm krypton-chlorine excilamp irradiation on Gram-positive and Gram-negative foodborne pathogenic bacteria.

The object of this study was to elucidate the bactericidal mechanism of a 222 nm Krypton Chlorine (KrCl) excilamp compared with that of a 254 nm Low Pressure mercury (LP Hg) lamp. The KrCl excilamp had higher bactericidal capacity against Gram-positive pathogenic bacteria (Staphylococcus aureus and L. monocytogenes) and Gram-negative pathogenic bacteria (S. Typhimurium and E. coli O157:H7) than did the LP Hg lamp when cell suspensions in PBS were irradiated with each type of UV lamp. It was found out that the KrCl excilamp induced cell membrane damage as a form of depolarization. From the study of respiratory chain dehydrogenase activity and the lipid peroxidation assay, it was revealed that cell membrane damage was attributed to inactivation of enzymes related to generation of membrane potential and occurrence of lipid peroxidation. Direct absorption of UV radiation which led to photoreaction through formation of an excited state was one of the causes inducing cell damage. Additionally, generation of ROS and thus occurrence of secondary damage can be another cause. The LP Hg lamp only induced damage to DNA but not to other components such as lipids or proteins. This difference was derived from differences of UV radiation absorption by cellular materials.