The biphasic activity of autophagy and heat shock protein response in peripheral blood mononuclear cells following acute resistance exercise in resistance-trained males.

PURPOSE: Autophagy and heat shock protein (HSP) response are proteostatic systems involved in the acute and adaptive responses to exercise. These systems may upregulate sequentially following cellular stress including acute exercise, however, currently few data exist in humans. This study investigated the autophagic and HSP responses to acute intense lower body resistance exercise in peripheral blood mononuclear cells (PBMCs) with and without branched-chain amino acids (BCAA) supplementation. METHODS: Twenty resistance-trained males (22.3 ± 1.5 yr; 175.4 ± .7 cm; 86.4 ± 15.6 kg) performed a bout of intense lower body resistance exercise and markers of autophagy and HSP70 were measured immediately post- (IPE) and 2, 4, 24, 48, and 72 h post-exercise. Prior to resistance exercise, 10 subjects were randomly assigned to BCAA supplementation of 0.22 g/kg/d for 5 days pre-exercise and up to 72 h following exercise while the other 10 subjects consumed a placebo (PLCB). RESULTS: There were no difference in autophagy markers or HSP70 expression between BCAA and PLCB groups. LC3II protein expression was significantly lower 2 and 4 h post-exercise compared to pre-exercise. LC3II: I ratio was not different at any time point compared to pre-exercise. Protein expression of p62 was lower IPE, 2, and 4 h post-exercise and elevated 24 h post-exercise. HSP70 expression was elevated 48 and 72 h post-exercise. CONCLUSIONS: Autophagy and HSP70 are upregulated in PBMCs following intense resistance exercise with autophagy increasing initially post-exercise and HSP response in the latter period. Moreover, BCAA supplementation did not affect this response.

The effect of repetition tempo on cardiovascular and metabolic stress when time under tension is matched during lower body exercise.

PURPOSE: To investigate the effect of repetition tempo on cardiovascular and metabolic stress when time under tension (TUT) and effort are matched during sessions of lower body resistance training (RT). METHODS: In a repeated-measures, cross-over design, 11 recreationally trained females (n = 5) and males (n = 6) performed 5 sets of belt squats under the following conditions: slow-repetition tempo (SLOW; 10 reps with 4-s eccentric and 2-s concentric) and traditional-repetition tempo (TRAD; 20 reps with 2-s eccentric and 1-s concentric). TUT (60 s) was matched between conditions and external load was adjusted so that lifters were close to concentric muscular failure at the end of each set. External load, total volume load (TVL), impulse (IMP), blood lactate, ratings of perceived exertion (RPE), HR, and muscle oxygenation were measured. RESULTS: Data indicated that TVL (p < 0.001), blood lactate (p = 0.017), RPE (p = 0.015), and HR (p < 0.001) were significantly greater during TRAD while external load (p = 0.030) and IMP (p = 0.002) were significantly greater during SLOW. Whether it was expressed as minimal values or change scores, muscle oxygenation was not different between protocols. CONCLUSION: When TUT is matched, TVL, cardiovascular stress, metabolic stress, and perceived exertion are greater when faster repetition tempos are used. In contrast, IMP and external load are greater when slower repetition tempos are used.

Aerobic Adaptations to Resistance Training: The Role of Time under Tension.

Generally, skeletal muscle adaptations to exercise are perceived through a dichotomous lens where the metabolic stress imposed by aerobic training leads to increased mitochondrial adaptations while the mechanical tension from resistance training leads to myofibrillar adaptations. However, there is emerging evidence for cross over between modalities where aerobic training stimulates traditional adaptations to resistance training (e.g., hypertrophy) and resistance training stimulates traditional adaptations to aerobic training (e.g., mitochondrial biogenesis). The latter is the focus of the current review in which we propose high-volume resistance training (i.e., high time under tension) leads to aerobic adaptations such as angiogenesis, mitochondrial biogenesis, and increased oxidative capacity. As time under tension increases, skeletal muscle energy turnover, metabolic stress, and ischemia also increase, which act as signals to activate the peroxisome proliferator-activated receptor gamma coactivator 1-alpha, which is the master regulator of mitochondrial biogenesis. For practical application, the acute stress and chronic adaptations to three specific forms of high-time under tension are also discussed: Slow-tempo, low-intensity resistance training, and drop-set resistance training. These modalities of high-time under tension lead to hallmark adaptations to resistance training such as muscle endurance, hypertrophy, and strength, but little is known about their effect on traditional aerobic training adaptations.

Metabolic Profile of Reciprocal Supersets in Young, Recreationally Active Women and Men.

ABSTRACT: Realzola, RA, Mang, ZA, Millender, DJ, Beam, JR, Bellovary, BN, Wells, AD, Houck, JM, and Kravitz, L. Metabolic profile of reciprocal supersets in young, recreationally active females and males. J Strength Cond Res 36(10): 2709-2716, 2022-Reciprocal supersets (RSSs) are a time-efficient style of resistance exercise (RE) that consist of performing 2 consecutive exercises with opposing muscle groups while limiting rest times between them. Previous research in men indicates a RSS has an increased physiological response when compared with traditional RE (TRAD). No between-sex comparison of metabolic data for RSSs exists. The purpose of this study was to create a metabolic profile for RSSs in men and women. Eighteen resistance-trained individuals underwent 2 bouts of volume-load equated RE: RSS and TRAD. Reciprocal superset exercises were split into 3 clusters: (a) hexagonal bar deadlift superset with leg press, (b) chest press superset with seated row, and (c) overhead dumbbell press superset with latissimus dorsi pull-downs. The TRAD protocol, doing the same exercises, emulated hypertrophy emphasis training. Oxygen uptake (V̇ o2 ), heart rate (HR), blood lactate ([BLa]), rate of perceived exertion (RPE), and excess post-exercise oxygen consumption (EPOC) were measured. Aerobic and anaerobic energy expenditure were estimated using V̇ o2 and lactate, respectively. The level of significance set for this study was p ≤ 0.05. Regardless of sex, a RSS elicited significantly greater average V̇ o2 , HR, [BLa], RPE, and anaerobic and aerobic energy expenditures, and was completed in a shorter time compared with TRAD ( p ≤ 0.05). When compared with women, men had significantly greater EPOC, average [BLa], and anaerobic and aerobic energy expenditures during RSSs ( p ≤ 0.05). The average [BLa] and aerobic energy expenditure of the men were also significantly greater than the women during TRAD ( p ≤ 0.05). This study suggests that a RSS is a metabolically demanding RE session that may elicit increases in musculoskeletal, cardiorespiratory, and physiological adaptations while decreasing the duration of exercise.

Comparison of Treadmill and Cycle Ergometer Exercise During Cardiac Rehabilitation: A Meta-analysis.

OBJECTIVE: To compare treadmill versus cycling-based exercise in cardiac rehabilitation (CR) on functional capacity (FC) outcomes. DATA SOURCE: Databases were searched for randomized studies using single modality continuous exercise. STUDY SELECTION: Studies implemented a continuous cycling or treadmill protocol for patients with either coronary artery disease (CAD) or chronic heart failure (CHF). The effect of single modality exercise on FC (VO2peak) was analyzed. Differences in the effect of CR on FC was assessed between the mode subgroup (cycling vs treadmill) and disease state subgroup (CAD vs CHF) within both the cycling and treadmill groups. DATA EXTRACTION: Data were extracted from 23 studies including 600 patients (mean age 60y, 86% men). DATA SYNTHESIS: There was a significant difference in effect size between studies that used cycling, Hedges' g=0.85 (95% confidence interval [95% CI], 0.52-1.17; k=13) and studies that used treadmill exercise, Hedges' g=0.46 (95% CI, 0.22-0.70; k=8). Within cycling studies (n=14), FC was higher among CAD patients, Hedges' g=1.03 (95% CI, 0.65-1.42; k=9) compared to those with CHF, Hedges' g=0.40 (95% CI, 0.09-0.71; k=4, P<.001). Conversely, among treadmill studies (n=9), FC was higher among CHF patients, Hedges' g=0.94 (95% CI, 0.23-1.65; k=2) compared to CAD, Hedges' g=0.33 (95% CI, 0.19-0.47; k=5; P<.01). CONCLUSIONS: According to identified studies, when cycling was the primary mode of exercise in CR, there was larger change in FC compared to treadmill exercise. In addition, CAD patients experienced greater gains in FC when cycling was the primary mode of exercise in CR, while CHF patients benefited more from treadmill-based exercise programs.

Metabolic effects of two high-intensity circuit training protocols: Does sequence matter?

BACKGROUND/OBJECTIVE: The integration of high-intensity interval training (HIIT) and circuit weight training (CWT) is seamless and practical for meeting recommended exercise guidelines. The purpose of this study was to determine the ideal combination of HIIT and CWT to elicit desired acute cardiorespiratory and metabolic responses in variables such as energy expenditure (EE), oxygen consumption (VO2), heart rate (HR), blood lactate (BLa-), excess post-exercise oxygen consumption (EPOC), rating of perceived exertion (RPE), and enjoyment. METHODS: Fourteen trained males (25.7 ±â€¯4.4 yr) completed two exercise protocols matched for volume and recovery periods. On one day, participants performed six HIIT bouts prior to three rounds of a nine exercise CWT protocol (HIC). The second day (separated by ≥ 72 h) consisted of three rounds of three mini-circuits (three exercises per circuit) integrated with three HIIT bouts between the first and second and second and third mini-circuits (TRI). VO2, HR, and EE were monitored throughout both protocols. EPOC for a 20-min duration, [BLa-] (five time points), RPE, and enjoyment were measured post-exercise. RESULTS: Energy expenditure was significantly higher during the HIC compared to the TRI protocol (p = .012), as well as EPOC (p = .034). [BLa-] was significantly greater immediate-, 5min-, 10min- and 20min-post-exercise following HIC as compared to TRI. Mean values for HIC and TRI were similar (p > .05) for HR and RPE. CONCLUSION: Performing HIIT prior to CWT elicits a higher metabolic perturbation compared to the TRI protocol. Although a significant EE difference was detected between the two trials, the practical difference (∼20 kcal) between protocols indicates both protocols are similarly effective for caloric expenditure, metabolic and cardiorespiratory response.

Telemedicine-Based Health Coaching Is Effective for Inducing Weight Loss and Improving Metabolic Markers.

BACKGROUND: To assess the efficacy of health coaching (HC) delivered through videoconferencing (VC) to favorably change physical activity (PA), weight, and metabolic markers in adults with high body mass index (BMI). MATERIALS AND METHODS: Thirty adults (BMI ≥30 kg/m2) were randomly assigned to one of three groups: VC, in-person (IP), or control group (CG). Participants received wireless watches and weight scales to sync with their personal smartphones; recorded data were wirelessly uploaded to a secure database. Participants assigned to VC and IP received individualized HC by a multidisciplinary team (registered dietitian, exercise physiologist, and medical doctor) based on data uploaded over the 12-week intervention. Steps/day and weight loss were analyzed through analyses of covariance. RESULTS: Within- and between-group changes in weight (kg), glucose, insulin, hemoglobin A1c (HbA1c), and Homeostasis Model Assessment estimate of insulin resistance (HOMA-IR) were analyzed through analyses of variance. Weight loss was greater (p < 0.05) for VC (8.23 ± 4.5 kg; 7.7%) than IP (3.2 ± 2.6 kg; 3.4%) and CG (2.9 ± 3.9 kg; 3.3%), respectively. Steps/day were significantly higher in VC than IP at week 4 and VC was significantly higher than the CG at weeks 6, 8, 9, and 11 (p ≤ 0.05). No within- or between-group differences were found for glucose, insulin, or HbA1C. HOMA-IR decreased for VC only (p ≤ 0.05). CONCLUSIONS: Our innovative, multidisciplinary, telemedicine HC delivered through VC led to more favorable changes in weight loss, PA (steps/day), and HOMA-IR than IP or no HC. VC may be an economical approach to improve health and promote behavior change in obese adults. CLINICAL TRIAL REGISTRATION NUMBER: ClinicalTrials.gov identifier NCT03278951.

Gait Retraining From Rearfoot Strike to Forefoot Strike does not change Running Economy.

Gait retraining is a method for management of patellofemoral pain, which is a common ailment among recreational runners. The present study investigated the effects of gait retraining from rearfoot strike to forefoot strike on running economy, heart rate, and respiratory exchange ratio immediately post-retraining and one-month post-retraining in recreational runners with patellofemoral pain. Knee pain was also measured. Sixteen participants (n=16) were randomly placed in the control (n=8) or experimental (n=8) group. A 10-minute treadmill RE test was performed by all subjects. The experimental group performed eight gait retraining running sessions where foot strike pattern was switched from rearfoot strike to forefoot strike, while the control group received no intervention. There were no significant differences for running economy (p=0.26), respiratory exchange ratio (p=0.258), or heart rate (p=0.248) between the groups. Knee pain reported on a visual analog scale was also significantly reduced (p<0.05) as a result of retraining. The present study demonstrates that retraining from rearfoot strike to forefoot strike did not affect running economy up to one-month post-retraining while reducing running-related patellofemoral pain.

The Effects of Eccentric Contraction Duration on Muscle Strength, Power Production, Vertical Jump, and Soreness.

Mike, JN, Cole, N, Herrera, C, VanDusseldorp, T, Kravitz, L, and Kerksick, CM. The effects of eccentric contraction duration on muscle strength, power production, vertical jump, and soreness. J Strength Cond Res 31(3): 773-786, 2017-Previous research has investigated the effects of either eccentric-only training or comparing eccentric and concentric exercise on changes related to strength and power expression, but no research to date has investigated the impact of altering the duration of either the concentric or the eccentric component on these parameters. Therefore, the purpose of this study was to assess the duration of eccentric (i.e., 2-second, 4-second vs. 6-second) muscle contractions and their effect on muscle strength, power production, vertical jump, and soreness using a plate-loaded barbell Smith squat exercise. Thirty college-aged men (23 ± 3.5 years, 178 ± 6.8 cm, 82 ± 12 kg, and 11.6 ± 5.1% fat) with 3.0 ± 1.0 years of resistance training experience and training frequency of 4.3 ± 0.9 days per week were randomized and assigned to 1 of 3 eccentric training groups that incorporated different patterns of contraction. For every repetition, all 3 groups used 2-second concentric contractions and paused for 1 second between the concentric and eccentric phases. The control group (2S) used 2-second eccentric contractions, whereas the 4S group performed 4-second eccentric contractions and the 6S group performed 6-second eccentric contractions. All repetitions were completed using the barbell Smith squat exercise. All participants completed a 4-week training protocol that required them to complete 2 workouts per week using their prescribed contraction routine for 4 sets of 6 repetitions at an intensity of 80-85% one repetition maximum (1RM). For all performance data, significant group × time (G × T) interaction effects were found for average power production across all 3 sets of a squat jump protocol (p = 0.04) while vertical jump did not reach significance but there was a trend toward a difference (G × T, p = 0.07). No other significant (p > 0.05) G × T interaction effects were found for the performance variables. All groups showed significant main effects for time in 1RM (p < 0.001), vertical jump (p = 0.004), peak power (p < 0.001), and average power (p < 0.001). Peak velocity data indicated that the 6S group experienced a significant reduction in peak velocity during the squat jump protocol as a result of the 4-week training program (p = 0.03). Soreness data revealed significant increases across time in all groups at both week 0 and week 4. Paired sample t-tests revealed greater differences in soreness values across time in the 2S group. The results provide further evidence that resistance training with eccentrically dominated movement patterns can be an effective method to acutely increase maximal strength and power expression in trained college age men. Furthermore, longer eccentric contractions may negatively impact explosive movements such as the vertical jump, whereas shorter eccentric contractions may instigate greater amounts of soreness. These are important considerations for the strength and conditioning professional to more fully understand that expressions of strength and power through eccentric training and varying durations of eccentric activity can have a significant impact for populations ranging from athletes desiring peak performance.

The Effect of Rest Interval Length on Upper and Lower Body Exercises in Resistance-Trained Females.

The purpose of this study was to investigate the effects of SHORT (1 min) and LONG (3 min) rest intervals (RI) on total volume lifted (TVL), repetition performance, fatigue index (FI), and blood lactate [La] during upper body (chest press) and lower body (leg press) exercise with low-intensity (75% of a 10-RM) in trained female lifters. Fourteen females (mean ± SD, age = 22.9 ± 5.4 years, training experience = 5.2 ± 2.5 years, height = 166.1 ± 6.9 cm, weight = 61.3 ± 5.1 kg, body fat % = 21.7 ± 3.3%) participated in this randomized, repeated-measures, cross-over design study. They performed four sets to failure on chest press (CP) and leg press (LP) under two conditions (SHORT and LONG RIs) in a counterbalanced manner. Paired-samples t-tests were used to analyze mean differences for TVL in CP and LP, separately. A 2 (exercise) x 2 (rest interval) repeated measures ANOVA was used to analyze mean differences in FI and average [La] values. A 2 (rest interval) x 4 (sets) repeated measures ANOVA was used to analyze mean differences in repetitions completed for each exercise. TVL for SHORT was significantly less when compared to LONG for both exercises. There was no significant difference in average [La] between RIs despite a greater FI in SHORT compared to LONG for both exercises. Lastly, [La] was higher during LP compared to CP irrespective of RI length. These results suggest that longer RIs are better for female lifters who want to optimize TVL with low-intensity resistance training. Metabolic stress, as measured by blood lactate, was greater during lower-body exercise.

Exercise-Based Cardiac Rehabilitation Improves Cognitive Function Among Patients With Cardiovascular Disease.

PURPOSE: To investigate the effects of cardiac rehabilitation (CR) exercise training on cognitive performance and whether the changes are associated with alterations in prefrontal cortex (PFC) oxygenation among patients with cardiovascular disease. METHODS: Twenty (men: n = 15; women: n = 5) participants from an outpatient CR program were enrolled in the study. Each participant completed a cognitive performance test battery and a submaximal graded treadmill evaluation on separate occasions prior to and again upon completion of 18 individualized CR sessions. A functional near-infrared spectroscopy (fNIRS) device was used to measure left and right prefrontal cortex (LPFC and RPFC) oxygenation parameters (oxyhemoglobin [O2Hb], deoxyhemoglobin [HHb], total hemoglobin [tHb], and oxyhemoglobin difference [Hbdiff]) during the cognitive test battery. RESULTS: Patients showed improvements in cardiorespiratory fitness (+1.4 metabolic equivalents [METs]) and various cognitive constructs. A significant increase in PFC oxygenation, primarily in the LPFC region, occurred at post-CR testing. Negative associations between changes in cognition (executive function [LPFC O2Hb: r = -0.45, P = .049; LPFC tHb: r = -0.49, P = .030] and fluid composite score [RPFC Hbdiff: r = -0.47, P = .038; LPFC Hbdiff: r = -0.45, P = .048]) and PFC changes were detected. The change in cardiorespiratory fitness was positively associated with the change in working memory score (r = 0.55, P = .016). CONCLUSION: Cardiovascular disease patients enrolled in CR showed significant improvements in multiple cognitive domains along with increased cortical activation. The negative associations between cognitive functioning and PFC oxygenation suggest an improved neural efficiency.

Exercise-Based Cardiac Rehabilitation Modulates Prefrontal Cortex Oxygenation during Submaximal Exercise Testing in Cardiovascular Disease Patients.

The purpose of this study was to investigate if prefrontal cortex (PFC) oxygenation during incremental exercise is altered among cardiovascular disease (CVD) patients who completed 6 weeks of exercise-based cardiac rehabilitation (CR). Nineteen (male = 14, female = 5; 65.5 ± 11.5 years) participants from an outpatient CR program were enrolled in the study. Each participant completed a submaximal graded treadmill evaluation at intake and again upon completion of 18 individualized CR sessions. Functional near-infrared spectroscopy (fNIRS) imaging was used to measure left- and right- PFC (LPFC and RPFC) oxygenation parameters during the submaximal exercise evaluations. Patients showed improvements in cardiorespiratory capacity (pre 5.5 ± 2.5 vs. post 6.9 ± 2.8 metabolic equivalents (METs)). A significant decrease in LPFC and RPFC oxygenation was observed during the post-CR exercise test compared to pre-CR. CVD patients enrolled in 6 weeks of CR showed significant improvements in functional capacity along with decreased cortical oxygenation during submaximal exercise. Exercise training may cause distribution of cortical resources to motor regions that support sustained exercise.

Acute Aerobic Exercise Based Cognitive and Motor Priming: Practical Applications and Mechanisms.

Acute exercise stimulates brain regions involved in motor and cognitive processes. Recent research efforts have explored the benefits of aerobic exercise on brain health and cognitive functioning with positive results reported for both healthy and neurocognitively impaired individuals. Specifically, exercise positioned near therapeutic (both behavioral and physical) activities may enhance outcomes associated with treatment outcomes (e.g., depression or motor skill) through neural plasticity promoting mechanisms (e.g., increased brain flow and oxygenation). This approach has been termed "exercise priming" and is a relatively new topic of exploration in the fields of exercise science and motor control. The authors report on physiological mechanisms that are related to the priming effect. In addition, parameters related to the exercise bout (e.g., intensity, duration) and the idea of combining exercise and therapeutic rehabilitation are explored. This exercise-based priming concept has the potential to be applied to many areas such as education, cognitive therapy, and motor rehabilitation.

Evaluation of the BOD POD for estimating percent body fat in collegiate track and field female athletes: a comparison of four methods.

This investigation examined the accuracy of the BOD POD on a group of Division I collegiate track and field female athletes (N = 30). Hydrostatic weighing (HW) was used as the gold standard method. Body density (Db) values obtained from the BOD POD (Db BP) were compared with those determined by HW (Db HW). Both Db values were converted to percent body fat (%BF) using the Siri equation for comparison. Percent body fat values obtained from the BOD POD (BF BP) were also compared with those obtained from dual-energy X-ray absorptiometry (DXA, BF DXA) and skinfold (SF, BF SF). The validity of the BOD POD was assessed using repeated-measures analysis of variance (ANOVA), and the relationship between the methods was examined through Pearson correlation. Average Db BP was 0.00890 g x cm(-3) lower (p < 0.05) than Db HW, resulting in a significant overestimation of %BF (p < 0.05) by the BOD POD. Values for BFDXA and BFBP also differed significantly (p < 0.05). On the other hand, BFSF and BF BP were not significantly different. The correlation between percent body fat values obtained from HW (BFHW) and BF BP was good (r = 0.88, SEE = 2.30) as well as between BF SF and BF BP (r = 0.85, SEE = 2.05). Conversely, the correlation between BFDXA and BF BP was poor (r = 0.25, SEE = 5.73). The strong correlation between BF BP and BF HW presented here suggests that the BOD POD has the potential to be used as a body composition analysis tool for female athletes. The advantages of the BOD POD over HW encourage further investigation of this instrument. However, the fact that the BOD POD and SF results did not differ significantly might suggest that the SF could be used in its place until a better rate of accuracy for this instrument is established.

Understanding the factors that effect maximal fat oxidation.

Lipids as a fuel source for energy supply during submaximal exercise originate from subcutaneous adipose tissue derived fatty acids (FA), intramuscular triacylglycerides (IMTG), cholesterol and dietary fat. These sources of fat contribute to fatty acid oxidation (FAox) in various ways. The regulation and utilization of FAs in a maximal capacity occur primarily at exercise intensities between 45 and 65% VO2max, is known as maximal fat oxidation (MFO), and is measured in g/min. Fatty acid oxidation occurs during submaximal exercise intensities, but is also complimentary to carbohydrate oxidation (CHOox). Due to limitations within FA transport across the cell and mitochondrial membranes, FAox is limited at higher exercise intensities. The point at which FAox reaches maximum and begins to decline is referred to as the crossover point. Exercise intensities that exceed the crossover point (~65% VO2max) utilize CHO as the predominant fuel source for energy supply. Training status, exercise intensity, exercise duration, sex differences, and nutrition have all been shown to affect cellular expression responsible for FAox rate. Each stimulus affects the process of FAox differently, resulting in specific adaptions that influence endurance exercise performance. Endurance training, specifically long duration (>2 h) facilitate adaptations that alter both the origin of FAs and FAox rate. Additionally, the influence of sex and nutrition on FAox are discussed. Finally, the role of FAox in the improvement of performance during endurance training is discussed.

Hemodynamic and metabolic responses to self-paced and ramp-graded exercise testing protocols.

Recent examinations have shown lower maximal oxygen consumption during traditional ramp (RAMP) compared with self-paced (SPV) graded exercise testing (GXT) attributed to differences in cardiac output. The current study examined the differences in hemodynamic and metabolic responses between RAMP and SPV during treadmill exercise. Sixteen recreationally trained men (aged23.7 ± 3.0 years) completed 2 separate treadmill GXT protocols. SPV consisted of five 2-min stages (10 min total) of increasing speed clamped by the Borg RPE6-20 scale. RAMP increased speed by 0.16 km/h every 15 s until volitional exhaustion. All testing was performed at 3% incline. Oxygen consumption was measured via indirect calorimetry; hemodynamic function was measured via thoracic impedance and blood lactate (BLa-) was measured via portable lactate analyzer. Differences between SPV and RAMP protocols were analyzed as group means by using paired-samples t tests (R Core Team 2017). Maximal values for SPV and RAMP were similar (p > 0.05) for oxygen uptake (47.1 ± 3.4 vs. 47.4 ± 3.4 mL·kg-1·min-1), heart rate (198 ± 5 vs. 200 ± 6 beats·min-1), ventilation (158.8 ± 20.7 vs. 159.3 ± 19.0 L·min-1), cardiac output (26.9 ± 5.5 vs. 27.9 ± 4.2 L·min-1), stroke volume (SV) (145.9 ± 29.2 vs. 149.8 ± 25.3 mL·beat-1), arteriovenous oxygen difference (18.5 ± 3.1 vs. 19.7 ± 3.1 mL·dL-1), ventilatory threshold (VT) (78.2 ± 7.2 vs. 79.0% ± 7.6%), and peak BLa- (11.7 ± 2.3 vs. 11.5 ± 2.4 mmol·L-1), respectively. In conclusion, SPV elicits similar maximal hemodynamic responses in comparison to RAMP; however, SV kinetics exhibited unique characteristics based on protocol. These results support SPV as a feasible GXT protocol to identify useful fitness parameters (maximal oxygen uptake, oxygen uptake kinetics, and VT).

Effect of Branched-Chain Amino Acid Supplementation on Recovery Following Acute Eccentric Exercise.

This study investigated the effect of branched-chain amino acid (BCAA) supplementation on recovery from eccentric exercise. Twenty males ingested either a BCAA supplement or placebo (PLCB) prior to and following eccentric exercise. Creatine kinase (CK), vertical jump (VJ), maximal voluntary isometric contraction (MVIC), jump squat (JS) and perceived soreness were assessed. No significant (p > 0.05) group by time interaction effects were observed for CK, soreness, MVIC, VJ, or JS. CK concentrations were elevated above baseline (p < 0.001) in both groups at 4, 24, 48 and 72 hr, while CK was lower (p = 0.02) in the BCAA group at 48 hr compared to PLCB. Soreness increased significantly from baseline (p < 0.01) in both groups at all time-points; however, BCAA supplemented individuals reported less soreness (p < 0.01) at the 48 and 72 hr time-points. MVIC force output returned to baseline levels (p > 0.05) at 24, 48 and 72 hr for BCAA individuals. No significant difference between groups (p > 0.05) was detected for VJ or JS. BCAA supplementation may mitigate muscle soreness following muscle-damaging exercise. However, when consumed with a diet consisting of ~1.2 g/kg/day protein, the attenuation of muscular performance decrements or corresponding plasma CK levels are likely negligible.

VO2max, protocol duration, and the VO2 plateau.

PURPOSE: The purpose of this study was to compare VO2max, VO2-time slopes at the end of the protocol (last 30 s), and the presence of a VO2 plateau (VO2-time slope < 0.05 L.min(-1) during the last 30 s) across four protocol durations (5, 8, 12, and 16 min) during incremental cycling exercise to VO2max. METHODS: Eight male (23.8 +/- 3.2 yr) and eight female (26.0 +/- 8.9 yr) subjects of moderate to high fitness levels participated in the study. RESULTS: VO2max was significantly higher in men than in women for each protocol duration, with main effect means of 4.23 versus 2.84 L.min(-1), respectively. For women, VO2max did not differ between any protocol duration. For men, VO2max for the 8-min protocol (4.44 +/- 0.39 L.min(-1)) was significantly higher than for all other protocol durations. Analysis of covariance, using the highest VO2max as the covariate, removed all protocol-duration significance for men. The VO2 slope for the final 30 s of each test was significantly lower for the 16-min protocol compared with the 5-min protocol, for both men and women. The ventilation threshold across four protocols was similar, at approximately 76% of VO2max for both men and women. CONCLUSIONS: The protocol duration of tests to VO2max should be between 8 and 10 min for healthy, moderately to highly trained subjects.