Benzothiazole-based novel fluorescence probe sensing 1, 3-diaminopropane.

Amines are extensively present in biological systems and are abundantly used in research, industries and agriculture. Systematic detection and quantification of certain amines can help us in food quality control and diagnosis of many diseases. A Schiff base probe HL was designed and successfully synthesized. It was proposed as a sensor for the exclusive detection of 1, 3- diaminopropane through turn-on fluorescence response in a variety of solvents including water. Micromolar limits of detection was achieved in all these solvents. Mechanism of detection was proposed by investigating mass spectrometric and NMR results. These were corroborated with DFT/TD-DFT calculations. Spiking experiments performed in various real water samples revealed the potential of the sensor to be used in day-to-day applications. Paper strip experiments demonstrated the suitability of the probe for real-life applications.

Real time sensor for Fe3+, Al3+, Cu2+ & PPi through quadruple mechanistic pathways using a novel dipodal quinoline-based molecular probe.

A quinoline-based small molecular probe, H2L was designed, synthesized and characterized by different spectroscopic methods. It was utilized as a multi-responsive probe for the detection of Fe3+, Al3+, Cu2+ and PPi. It showed very selective instant turn-on fluorimetric response towards Fe3+and Al3+ with a detection limit in nanomolar range. Solutions of H2L containing Fe3+ or Al3+ could sequentially sense PPi by a turn-off mechanism. Also, H2L could determine the presence of Cu2+ very selectively among a series of other metal ions by a sharp change in colour. Detection of Cu2+ through colorimetry was further investigated by systematic UV-Vis studies and the potential of H2L to act as a potential colorimetric sensor for Cu2+ was suitably established. Filter-paper strip experiments were conducted to demonstrate the practical utility of the proposed sensor. Potential applications of H2L as a sensor for pH in the acidic range has also been explored.

Poly-beta-hydroxybutyrate accumulation in Nostoc muscorum: effects of metabolic inhibitors.

Poly-beta-hydroxybutyrate (PHB) accumulation in Nostoc muscorum was studied in presence of various metabolic inhibitors. Supplementation of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) was found to suppress PHB accumulation in phosphate-limited N. muscorum under photoautotrophic growth condition. PHB accumulation increased up to 21% and 17% from an initial PHB content of 8.5% of dry weight, respectively, under carbonylcyanide m-chlorophenylhydrazone (CCCP) and dicyclohexylcarbodiimide (DCCD) treatment, whereas 2,4 -dinitrophenol (DNP) supplementation depicted insignificant effect on PHB pool of the test cyanobacterium. Supplementation of l-methionine-dl-sulfoximine (MSX) and azaserine was also found to increase PHB accumulation in N(2) -fixing and NH(4)(+) -grown N. muscorum, but not in NO(3)(-) -grown cells. The stimulatory action of monofluoroacetate on PHB accumulation was suppressed in presence of alpha-ketoglutarate and DCMU. Interestingly, 2,3 -butanedione supplementation was not only found inhibitory for accumulation of PHB in P-deficient, N-deficient and chemoheterotrophically grown N. muscorum but suppression of PHB synthesis was also evident in control cultures in presence of 2,3 -butanedione. The possible mechanisms are discussed.

Process optimization for poly-beta-hydroxybutyrate production in a nitrogen fixing cyanobacterium, Nostoc muscorum using response surface methodology.

A five-level-four-factor central composite rotary design was employed to find out the interactive effects of four variables, viz. concentrations of acetate, glucose and K2HPO4, and dark incubation period on poly-beta-hydroxybutyrate (PHB) production in a N2-fixing cyanobacterium, Nostoc muscorum. Acetate, glucose and dark incubation period exhibited positive impacts on PHB yield. Using response surface methodology (RSM), a second order polynomial equation was obtained by multiple regression analysis. A yield of 45.6% of dry cell weight (dcw) was achieved at reduced level of nutrients, i.e. 0.17% acetate, 0.16% glucose and 5 mg l(-1) K2HPO4 at a dark incubation period of 95 h as compared to 41.6% PHB yield in 0.4% acetate, 0.4% glucose and 40 mg l(-1) K2HPO4 at a dark incubation period of 168 h under single factor optimization strategy.

Structure and properties of bivalent nickel and copper complexes with pyrazine-amide-thioether coordination: stabilization of trivalent nickel.

Acyclic pyrazine-2-carboxamide and thioether containing hexadentate ligand 1,4-bis[o-(pyrazine-2-carboxamidophenyl)]-1,4-dithiobutane (H(2)bpzctb), in its deprotonated form, has afforded light brown [Ni(II)(bpzctb)](1)(S=1) and green [Cu(II)(bpzctb)](2)(S=1/2) complexes. The crystal structures of 1.CH(3)OH and 2.CH(2)Cl(2) revealed that in these complexes the ligand coordinates in a hexadentate mode, affording examples of distorted octahedral M(II)N(2)(pyrazine)N'(2)(amide)S(2)(thioether) coordination. Each complex exhibits in CH(2)Cl(2) a reversible to quasireversible cyclic voltammetric response, corresponding to the Ni(III)/Ni(II)(1) and Cu(II)/Cu(I)(2) redox process. The E(1/2) values reveal that the complexes of bpzctb(2-) are uniformly more anodic by approximately 0.2 V than those of the corresponding complexes with the analogous pyridine ligand, 1,4-bis[o-(pyridine-2-carboxamidophenyl)]-1,4-dithiobutane (H(2)bpctb), attesting that compared to pyridine, pyrazine is a better stabilizer of the Ni(ii) or Cu(i) state. Coulometric oxidation of the previously reported complex [Ni(II)(bpctb)] and 1 generates [Ni(III)(bpctb)](+) and [Ni(III)(bpzctb)](+) species, which exhibit a LMCT transition in the 470--480 nm region and axial EPR spectra corresponding to a tetragonally elongated octahedral geometry. Complex 2 exhibits EPR spectra characteristic of the d(z(2)) ground state.