RESUMO
Taste information from type III taste cells to gustatory neurons is thought to be transmitted via synapses. However, the molecular mechanisms underlying taste transduction through this pathway have not been fully elucidated. In this study, to identify molecules that participate in synaptic taste transduction, we investigated whether complexins (Cplxs), which play roles in regulating membrane fusion in synaptic vesicle exocytosis, were expressed in taste bud cells. Among four Cplx isoforms, strong expression of Cplx2 mRNA was detected in type III taste cells. To investigate the function of CPLX2 in taste transduction, we observed taste responses in CPLX2-knockout mice. When assessed with electrophysiological and behavioral assays, taste responses to some sour stimuli in CPLX2-knockout mice were significantly lower than those in wild-type mice. These results suggested that CPLX2 participated in synaptic taste transduction from type III taste cells to gustatory neurons. A part of taste information is thought to be transmitted via synapses. However, the molecular mechanisms have not been fully elucidated. To identify molecules that participate in synaptic taste transduction, we investigated complexins (Cplxs) expression in taste bud cells. Strong expression of Cplx2 mRNA was detected in taste bud cells. Furthermore, taste responses to some sour stimuli in CPLX2- knockout mice were significantly lower than those in wild-type mice. These suggested that CPLX2 participated in synaptic taste transduction.
Assuntos
Proteínas Adaptadoras de Transporte Vesicular/metabolismo , Proteínas do Tecido Nervoso/metabolismo , Papilas Gustativas/metabolismo , Paladar/fisiologia , Animais , Exocitose/fisiologia , Hibridização In Situ , Mecanotransdução Celular , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Sinapses/metabolismoRESUMO
Crystallization of eukaryotic membrane proteins is a challenging, iterative process. The protein of interest is often modified in an attempt to improve crystallization and diffraction results. To accelerate this process, we took advantage of a GFP-fusion yeast expression system that uses PCR to direct homologous recombination and gene cloning. We explored the possibility of employing more than one PCR fragment to introduce various mutations in a single step, and found that when up to five PCR fragments were co-transformed into yeast, the recombination frequency was maintained as the number of fragments was increased. All transformants expressed the model membrane protein, while the resulting plasmid from each clone contained the designed mutations only. Thus, we have demonstrated a technique allowing the expression of mutant membrane proteins within 5 days, combining a GFP-fusion expression system and yeast homologous recombination.
Assuntos
Clonagem Molecular/métodos , Proteínas de Membrana/biossíntese , Proteínas Recombinantes/biossíntese , Saccharomyces cerevisiae/genética , Animais , Proteínas de Fluorescência Verde/genética , Proteínas de Membrana/genética , Camundongos , Mutação , Reação em Cadeia da Polimerase , Biossíntese de Proteínas , Proteínas Recombinantes/genética , Recombinação Genética , Canais de Cátion TRPM/biossíntese , Canais de Cátion TRPM/genética , Transformação GenéticaRESUMO
Previous studies have shown that aging modifies taste sensitivity. However, the factors affecting the changes in taste sensitivity remain unclear. To investigate the cause of the age-related changes in taste sensitivity, we compared the peripheral taste detection systems in young and old mice. First, we examined whether taste sensitivity varied according to age using behavioral assays. We confirmed that the taste sensitivities to salty and bitter tastes decreased with aging. In other assays, the gustatory nerve responses to salty and sweet tastes increased significantly with aging, while those to bitter taste did not change. Thus, the profile of the gustatory nerve responses was inconsistent with the profile of the behavioral responses. Next, we evaluated the expressions of taste-related molecules in the taste buds. Although no apparent differences in the expressions of representative taste receptors were observed between the two age groups, the mRNA expressions of signaling effectors were slightly, but significantly, decreased in old mice. No significant differences in the turnover rates of taste bud cells were observed between the two age groups. Thus, we did not observe any large decreases in the expressions of taste-related molecules and turnover rates of taste bud cells with aging. Based on these findings, we conclude that changes in taste sensitivity with aging were not caused by aging-related degradation of peripheral taste organs. Meanwhile, the concentrations of several serum components that modify taste responses changed with age. Thus, taste signal-modifying factors such as serum components may have a contributing role in aging-related changes in taste sensitivity.
Assuntos
Vias Aferentes/fisiologia , Envelhecimento/fisiologia , Papilas Gustativas/fisiologia , Percepção Gustatória/fisiologia , Paladar/fisiologia , Animais , Animais Recém-Nascidos , Canais de Cálcio/genética , Canais de Cálcio/metabolismo , Nervo da Corda do Tímpano/fisiologia , Relação Dose-Resposta a Droga , Regulação da Expressão Gênica no Desenvolvimento , Canal de Potássio KCNQ1/genética , Canal de Potássio KCNQ1/metabolismo , Masculino , Camundongos , Motivação , Fosfolipase C beta/genética , Fosfolipase C beta/metabolismo , Receptores de Superfície Celular/genética , Receptores de Superfície Celular/metabolismo , Receptores Acoplados a Proteínas G/genética , Receptores Acoplados a Proteínas G/metabolismo , Transdução de Sinais/efeitos dos fármacos , Transdução de Sinais/genética , Estimulação Química , Transducina/genética , Transducina/metabolismoRESUMO
Taste cells release neurotransmitters to gustatory neurons to transmit chemical information they received. Sweet, umami, and bitter taste cells use ATP as a neurotransmitter. However, ATP release from sour taste cells has not been observed so far. Instead, they release serotonin when they are activated by sour/acid stimuli. Thus it is still controversial whether sour taste cells use ATP, serotonin, or both. By reverse transcription-polymerase chain reaction and subsequent in situ hybridization (ISH) analyses, we revealed that of 14 serotonin receptor genes only 5-HT3A and 5-HT3B showed significant/clear signals in a subset of neurons of cranial sensory ganglia in which gustatory neurons reside. Double-fluorescent labeling analyses of ISH for serotonin receptor genes with wheat germ agglutinin (WGA) in cranial sensory ganglia of pkd1l3-WGA mice whose sour neural pathway is visualized by the distribution of WGA originating from sour taste cells in the posterior region of the tongue revealed that WGA-positive cranial sensory neurons rarely express either of serotonin receptor gene. These results suggest that serotonin receptors expressed in cranial sensory neurons do not play any role as neurotransmitter receptor from sour taste cells.
Assuntos
Gânglios Sensitivos/metabolismo , Receptores de Serotonina/metabolismo , Crânio/inervação , Animais , Expressão Gênica , Masculino , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Receptores Purinérgicos P2X2/genética , Receptores Purinérgicos P2X2/metabolismo , Receptores de Serotonina/genética , Receptores 5-HT3 de Serotonina/genética , Receptores 5-HT3 de Serotonina/metabolismo , Células Receptoras Sensoriais/metabolismo , PaladarRESUMO
Polycystic kidney disease (PKD) 2L1 protein is a member of the transient receptor potential (TRP) ion channel family. In circumvallate and foliate papillae, PKD2L1 is coexpressed with PKD1L3. PKD2L1 and PKD1L3 interact through their transmembrane domain and the resulting heteromer PKD1L3/PKD2L1 owns a unique channel property called 'off-responses' to acid stimulation, although PKD2L1 does not own this property by itself. To define the pharmacological properties of the PKD1L3/PKD2L1 channel, we developed a new method to effectively evaluate channel activity using human embryonic kidney 293T cells in which the channel was heterologously expressed. This method was applied to screen substances that potentially regulate it. We found that capsaicin and its analogs, which are TRPV1 agonists, inhibited the response to acid stimuli and that the capsaicin inhibition was reversible with an IC(50) of 32.5 µm. Capsaicin and its analogs are thus useful tools for physiological analysis of PKD1L3/PKD2L1 function.
Assuntos
Capsaicina/farmacologia , Receptores de Superfície Celular/antagonistas & inibidores , Fármacos do Sistema Sensorial/farmacologia , Canais de Cátion TRPP/antagonistas & inibidores , Animais , Canais de Cálcio/metabolismo , Células Cultivadas , Células HEK293 , Humanos , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Dados de Sequência Molecular , Receptores de Superfície Celular/metabolismo , Canais de Cátion TRPP/metabolismo , Papilas Gustativas/efeitos dos fármacos , Língua/metabolismoRESUMO
Taste loss is a major cause of morbidity in patients undergoing head and neck irradiation. Previous studies have reported the alteration of the four basic tastes in patients with head and neck cancer during radiotherapy. However, only a few studies have been conducted on the effects of irradiation on the function of umami taste, a novel and basic taste recently recognized. In a prospective study, 52 patients undergoing radical head and neck irradiation were assessed for taste loss. Taste ability was measured by the taste threshold for umami quality using the whole-mouth taste method in patients before, during, and immediately after radiotherapy. Umami taste declined of the 3rd week after the start of radiotherapy and improved of the 8th week.