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1.
J AOAC Int ; 98(1): 62-70, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25857880

RESUMO

A ready-made dry medium method for coliform count, the Medi·Ca CC method, was compared to the Violet Red Bile Agar method (Bacteriological Analytical Manual, Chapter 4, Enumeration of Escherichia coli and the Coliform Bacteria, Section G) for nine raw foods from four food categories: raw ground pork, raw lamb, raw ground chicken, raw tuna fillet, raw salmon fillet, raw shrimp, fresh peeled banana, fresh cut pineapple, and fresh cut apple. The 95% confidence interval for the mean difference between the two methods at each contamination level for seven matrixes from all four categories fell within the range of -0.50 to 0.50, and no statistical difference was observed at all three contamination levels for four matrixes from three categories. These results demonstrated that the Medi·Ca CC method is a reasonable alternative to the reference method for raw meat, raw poultry, raw fish, and fresh fruits.


Assuntos
Contagem de Colônia Microbiana/métodos , Enterobacteriaceae/isolamento & purificação , Carne/microbiologia , Animais , Galinhas , Microbiologia de Alimentos , Frutas/química , Salmão , Ovinos , Suínos , Atum
2.
J AOAC Int ; 101(5): 1522-1533, 2018 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-29673418

RESUMO

A ready-made dry medium method for Staphylococcus aureus count, the Medi·Ca SA method incubated at 35 or 37°C, was compared with the Baird-Parker method (AOAC Official MethodSM 975.55) for 11 food matrices: raw beef, raw ground beef, raw lamb, cooked ham, raw salmon, frozen prawn, fresh chilled pasta, pasteurized milk, natural cheese, cream puff, and potato salad. The mean difference between the two methods at each contamination level for each matrix was <0.5 log10, and the 95% confidence intervals on the mean differences fell within the range of -0.50 to 0.50. Standard deviation of repeatability and RSDr values of the Medi·Ca SA method were generally the same level as those of the Baird-Parker method, and r2 ranged from 0.98 to 1.00. Product consistency and stability studies showed little variability between productions lots and a shelf-life of 16 months. Incubation time within the range of 22-26 h and variations to the sample volume did not adversely affect the results. These results showed that the Medi·Ca SA method is a reasonable alternative to the reference method for selected food matrices and makes it possible to simultaneously detect and enumerate S. aureus in only 24 h.


Assuntos
Contagem de Colônia Microbiana/métodos , Análise de Alimentos/métodos , Staphylococcus aureus/isolamento & purificação , Animais , Bovinos , Contagem de Colônia Microbiana/instrumentação , Laticínios/microbiologia , Desenho de Equipamento , Análise de Alimentos/instrumentação , Microbiologia de Alimentos , Humanos , Leite/microbiologia , Carne Vermelha/microbiologia , Salmão/microbiologia , Ovinos , Intoxicação Alimentar Estafilocócica/microbiologia , Suínos
3.
J AOAC Int ; 100(2): 474-484, 2017 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-28118573

RESUMO

A ready-made dry medium method for Escherichia coli and coliform count, the Medi·Ca EC method, was compared with the most probable number (MPN) method using Brilliant Green Lactose Bile broth and E. coli broth (AOAC INTERNATIONAL Official MethodSM 966.24) for seven food matrixes: raw beef, raw pork, raw frozen pork, raw lamb, raw salmon, frankfurter sausage, and cooked ham. The mean difference between the two methods at each contamination level for each matrix was <0.5 log10, and the 95% confidence intervals for the mean differences fell within the range of -0.5 to 0.5, with the exception of a few cases in the independent laboratory study. sr and RSDr values of the Medi·Ca EC method were generally lower than those of the MPN method, and r2 ranged from 0.91 to 0.99. Product consistency and stability studies showed little variability between production lots and the shelf-life of 20 months. An incubation time within the range of 22-26 h did not adversely affect the results; however, variations in sample volume did affect final counts. These results showed that the Medi·Ca EC method is a reasonable alternative to the reference method for the selected food matrixes and makes it possible to simultaneously detect and enumerate E. coli and coliform in only 24 h.


Assuntos
Enterobacteriaceae/isolamento & purificação , Carne Vermelha/microbiologia , Alimentos Marinhos/microbiologia , Carga Bacteriana , Meios de Cultura , Contaminação de Alimentos , Estatística como Assunto
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