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1.
Skin Health Dis ; 3(5): e270, 2023 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-37799366

RESUMO

Aplasia cutis congenita (ACC) is defined as complete or partial loss or absence of skin at birth and it can occur on any part of the body, but most commonly on the scalp. Single offspring with ACC have been reported in most case reports, but cases in twins are rarely reported. Here, we report two cases of ACC, monozygotic twin boys presented with scattered skin absence over the scalp vertex after birth. All the lesions presented as ulcers with no hair and healed with scars, otherwise, the twins were well developed mentally and physically. In addition, the whole exome sequencing of the twins and their parents might provide diagnosis and classification assistance.

2.
J Sep Sci ; 35(2): 256-62, 2012 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-25939902

RESUMO

Recycling counter-current chromatography (CCC) together with step-gradient CCC and medium-pressure liquid chromatography (MPLC) was employed to separate nine anthraquinone compounds from Cassia obtusifolia L. in this study. The results showed that recycling CCC is a powerful tool for compounds that are difficult to separate with common elution mode. CCC was the better option for crude material while MPLC had advantage for the final tuning. The combination of recycling CCC and MPLC could simplify the method exploring process in the separation process. The structures of these compounds were identified according to their mass spectra, by (1)H-NMR and compared with standard compounds.


Assuntos
Antraquinonas/isolamento & purificação , Cassia/química , Distribuição Contracorrente/métodos , Antraquinonas/química , Cromatografia Líquida de Alta Pressão/métodos , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/isolamento & purificação , Espectroscopia de Ressonância Magnética , Plantas Medicinais/química , Sementes/química , Solventes , Espectrometria de Massas por Ionização por Electrospray
3.
Arch Pharm (Weinheim) ; 345(12): 945-56, 2012 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22949330

RESUMO

A series of 4ß-[(4-substituted)-1,2,3-triazol-1-yl]podophyllotoxin congeners were synthesized by employing click chemistry and further evaluated for their antitumor activity by MTT assay. Among them, six congeners (10, 11, 12, 13, 22, and 24) exhibited approximately 100-fold more potent inhibitory activity against four tumor cell lines (HepG2, MKN-45, NCI-H1993, and B16) than etoposide as positive control. Docking studies on binding in the ATPase domain of topoisomerase II revealed perfect docking of four congeners in the active site. Furthermore, the podophyllotoxin congeners 10, 11, 12, and 13 induced cell cycle arrest of HepG2 cells at the G(2) /M phase in a concentration-dependent manner, assessed by flow cytometric analysis, highlighting that they exert their antitumor activity via HepG2 cell apoptosis.


Assuntos
Antineoplásicos/síntese química , Podofilotoxina/análogos & derivados , Podofilotoxina/síntese química , Triazóis/síntese química , Antineoplásicos/química , Antineoplásicos/farmacologia , Ciclo Celular/efeitos dos fármacos , Química Click , Ensaios de Seleção de Medicamentos Antitumorais , Células Hep G2 , Humanos , Concentração Inibidora 50 , Simulação de Acoplamento Molecular , Estrutura Molecular , Podofilotoxina/farmacologia , Relação Estrutura-Atividade , Triazóis/química , Triazóis/farmacologia
4.
Immunol Res ; 68(3): 177, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32529461

RESUMO

The order of authors' affiliations in the published version of this article was not consistent with the order in the submitted manuscript due to typesetting.

5.
Immunol Res ; 67(2-3): 202-211, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-31292886

RESUMO

B7-H3 is a cell surface molecule in the immunoglobulin superfamily that has been shown to perform both immunological and non-immunological functions. It has also been found that vascular endothelial growth factor (VEGF) is an important molecule in the modulation of endothelial cell behavior. In this study, we analyzed the serum expression of B7-H3 in 113 rheumatoid arthritis and systemic lupus erythematous patients using the ELISA and found a positive correlation between B7-H3 and VEGF. Next, we investigated the involvement of B7-H3 in angiogenesis using human umbilical vein endothelial cells (HUVECs) with transient knockdown of B7-H3 and an in vivo Matrigel model. Data from the in vitro experiments showed that B7-H3 increased cell proliferation, migration, and tube formation, and correlated with the expression of VEGF. Furthermore, B7-H3 affected the formation of functional vascular networks in Matrigel plugs, which were dissected from mice injected with different HUVECs. Our data suggest that B7-H3 promotes angiogenesis through the enhancement of VEGF secretion. This is the first study proposing a significant role for B7-H3 in the promotion of angiogenesis and may provide further understanding of this gene's biological function.


Assuntos
Antígenos B7/metabolismo , Células Endoteliais/metabolismo , Neovascularização Fisiológica , Fator A de Crescimento do Endotélio Vascular/metabolismo , Artrite Reumatoide/sangue , Artrite Reumatoide/etiologia , Artrite Reumatoide/metabolismo , Antígenos B7/sangue , Antígenos B7/genética , Biomarcadores , Movimento Celular , Proliferação de Células , Células Cultivadas , Imunofluorescência , Células Endoteliais da Veia Umbilical Humana , Humanos , Lúpus Eritematoso Sistêmico/sangue , Lúpus Eritematoso Sistêmico/etiologia , Lúpus Eritematoso Sistêmico/metabolismo , Neovascularização Fisiológica/genética
7.
Onco Targets Ther ; 11: 7635-7642, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30464512

RESUMO

BACKGROUND: (PD-L2), a ligand of programmed cell death protein 1 (PD-1), is an inhibitory receptor of T cells and activated B cells. Many studies have focused on PD-L1, another ligand of PD-1, and the prognostic significance of PD-L1 has been reported in many tumors. However, the expression of PD-L2 in relation to clinical outcomes has not been fully investigated in cancer patients. PATIENTS AND METHODS: In this study, we investigated the expression of PD-L2 via immunohistochemistry (IHC) in the pathological specimens of 348 patients treated for colorectal cancer (CRC). RESULTS: Strong PD-L2 expression was found in the cancer tissues from 41% of the CRC patients who also had a high TNM stage and carcinoembryonic antigen (CEA) concentration. We also carried out functional studies in vitro, which showed that PD-L2 did not influence the growth of the CRC cell line HCT116, but increased cell invasion. CONCLUSION: Collectively, these findings suggest that PD-L2 may be a potential therapeutic target for CRC.

8.
J Immunol Res ; 2017: 5728512, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29423417

RESUMO

Both membrane-bound and soluble forms of costimulatory molecules play important roles in immune-regulatory networks. B7-H3, a member of the B7 family, has been found with aberrant expression in tumors and infectious disease. However, the significance of sB7-H3 expression in systemic lupus erythematosus (SLE) has not been investigated. Using the peripheral blood of 78 SLE patients, we established a comprehensive database containing clinical data and relevant laboratory tests. We found that sB7-H3 expression in SLE patients was significantly lower compared with the healthy individuals. In addition, sB7-H3 levels in the patients were positively correlated with the disease activity as indicated by SLE disease activity index score, rashes, fever, and inflammatory cytokines. Moreover, sB7-H3 was associated with the counts of red blood cells and hemoglobin. Our findings suggest that sB7-H3 might counteract the aberrant immune response and potentially serve as a monitoring indicator of disease progression and therapeutic target in SLE treatment.


Assuntos
Antígenos B7/genética , Eritrócitos/patologia , Lúpus Eritematoso Sistêmico/imunologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Antígenos B7/sangue , Citocinas/metabolismo , Progressão da Doença , Regulação para Baixo , Feminino , Hemoglobinas/metabolismo , Humanos , Mediadores da Inflamação/metabolismo , Masculino , Pessoa de Meia-Idade , Índice de Gravidade de Doença , Adulto Jovem
9.
J Chromatogr A ; 1295: 48-56, 2013 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-23618226

RESUMO

A general approach based on stable isotope labeling and UPLC/Q-TOF-MS analysis of in vivo novel metabolites of honokiol has been developed in our study. In this method, urine samples were collected after intravenous administration of mixture of regular and [(13)C6]-labeled honokiol at 1:1 ratio to healthy rats. The metabolites could be easily recognized by the determination of a chromatographically co-eluted pair of isotopomers (MS doublet peaks) with similar peak intensities and mass difference corresponding to that between isotope-labeled and non-isotope-labeled honokiol. A total of 51 metabolites were detected, 37 of which were tentatively identified based on mass accuracy (<5 ppm). Among them, 33 of honokiol metabolites were first reported with 5 metabolites belonging to phase I and other 32 metabolites belonging to phase II metabolites. Our results highlighted that the main phase I metabolic pathways of honokiol in rats were oxidation, and the phase II metabolic pathways were sulfation, glucuronidation, acetylation as well as amino acids conjugation. This was the first research focused on the biotransformation of honokiol in rats, and the identification of these metabolites might provide us essential information for further pharmacological and clinical studies of honokiol.


Assuntos
Compostos de Bifenilo/química , Cromatografia Líquida/métodos , Medicamentos de Ervas Chinesas/química , Lignanas/química , Magnolia/química , Espectrometria de Massas em Tandem/métodos , Animais , Biotransformação , Compostos de Bifenilo/metabolismo , Medicamentos de Ervas Chinesas/metabolismo , Marcação por Isótopo , Lignanas/metabolismo , Masculino , Ratos , Ratos Wistar
10.
Artigo em Inglês | MEDLINE | ID: mdl-23792368

RESUMO

Honokiol, a natural molecule isolated from Magnolia officinalis Rehd. et Wils., is widely known as an antitumor agent. In present work, an analysis of in vivo biotransformation and metabolites of honokiol has been performed by a combined method based on stable isotope cluster technique with honokiol-[(13)C6]-labeled and ultra-high performance liquid chromatography/quadrupole-time-of-flight-mass spectrometry (UHPLC/Q-TOF-MS). The metabolites could be easily identified by the determination of a chromatographically co-eluted pair of isotopomers (MS doublet peaks) with similar peak intensities and mass difference corresponding to that between isotope-labeled and non-isotope-labeled honokiol. A total of eighteen metabolites were detected and tentatively identified, fourteen of which were reported for the first time. The results indicated that the main metabolic pathways of honokiol in rats were hydroxylation, methylation, sulfation and glucuronidation. This study provided the first essential information on biotransformation and metabolites of honokiol in rats, which was very useful for further pharmacological and clinical studies of honokiol as a potent drug candidate.


Assuntos
Compostos de Bifenilo/metabolismo , Cromatografia Líquida de Alta Pressão/métodos , Lignanas/metabolismo , Espectrometria de Massas/métodos , Animais , Compostos de Bifenilo/sangue , Compostos de Bifenilo/química , Glucuronídeos/sangue , Glucuronídeos/química , Glucuronídeos/metabolismo , Glutamatos/sangue , Glutamatos/química , Glutamatos/metabolismo , Marcação por Isótopo , Lignanas/sangue , Lignanas/química , Masculino , Redes e Vias Metabólicas , Ratos , Ratos Sprague-Dawley
11.
J Med Chem ; 55(22): 9958-72, 2012 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-23025244

RESUMO

Nonalcoholic fatty liver disease (NAFLD), one of chronic liver diseases, seems to be rising as the obesity epidemic continues. In this study, 54 novel (thio)barbituric acid derivatives have been synthesized and evaluated for pharmacological activity. 7h exhibited potent glucose-lowering effects on insulin-resistant HepG2 cells and regulated adiponectin and leptin expression in 3T3-L1 adipocytes. Oral administration of 7h at 25 mg kg(-1) day(-1) for 4 weeks improved the progression of high fat diet-induced NAFLD by reducing the weight of body, liver, and fat, as well as modulating serum levels of fasting glucose, insulin, triglycerides, LDL-c, ALT, adiponectin and hepatic contents of triglycerides, total cholesterol. H&E stainings revealed that 7h blocked fat deposition in liver and the increase of adipocyte number and size in adipose tissues from NAFLD. Furthermore, treatment with 7h alleviated the obese clinical symptoms, recovered serum biomarkers to appropriate ranges, and improved glucose tolerance by OGTT and IGTT in DIO mice.


Assuntos
Adipócitos/efeitos dos fármacos , Barbitúricos/farmacologia , Dieta Hiperlipídica/efeitos adversos , Fígado Gorduroso/tratamento farmacológico , Obesidade/etiologia , Piperidinas/farmacologia , Pirimidinas/farmacologia , Células 3T3-L1 , Adipócitos/citologia , Adiponectina/metabolismo , Alanina Transaminase/metabolismo , Animais , Barbitúricos/síntese química , Barbitúricos/farmacocinética , Peso Corporal/efeitos dos fármacos , Colesterol/metabolismo , Modelos Animais de Doenças , Fígado Gorduroso/etiologia , Fígado Gorduroso/metabolismo , Feminino , Glucose/metabolismo , Teste de Tolerância a Glucose , Células Hep G2 , Humanos , Insulina/metabolismo , Resistência à Insulina , Leptina/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Hepatopatia Gordurosa não Alcoólica , Piperidinas/síntese química , Piperidinas/farmacocinética , Pirimidinas/síntese química , Pirimidinas/farmacocinética , Ratos , Ratos Sprague-Dawley , Ratos Wistar , Distribuição Tecidual , Triglicerídeos/metabolismo
12.
Fitoterapia ; 83(8): 1402-8, 2012 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22902267

RESUMO

The aim of this study is to investigate the cytotoxic and apoptotic effects of constituents from the seeds of Millettia pachycarpa Benth. Fourteen compounds (1-14) including one novel chalcone (10) were isolated as active principles from Chinese herbal medicine M. pachycarpa Benth. Their structures were identified by using spectroscopic methods. All isolates were then evaluated for their cytotoxic effects against several cancer cell lines (HepG2, C26, LL2 and B16) with cisplatin as a positive control. And their apoptosis-inducing effects were tested against HeLa-C3 cells with taxol as a positive control. Both studies showed that compounds 1, 2, 7 and 10 demonstrated significant cytotoxic and apoptotic effects against cancer cells. Moreover, in the apoptosis assay the novel chalcone (10) showed strong apoptosis inducing effects at a concentration of 2µM within 36h. It was found to be the most potent apoptotic inducer of the compounds isolated from M. pachycarpa Benth.


Assuntos
Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/farmacologia , Apoptose/efeitos dos fármacos , Millettia/química , Linhagem Celular Tumoral , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/farmacologia , Humanos , Estrutura Molecular
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