Emotional experiences of ghosting.

Although ghosting (i.e., unilaterally ending a relationship by ceasing communication) has only recently entered the lexicon, it is a regularly used form of relationship dissolution. However, little research has examined the emotional experiences of ghosting, particularly the experiences of those on both sides of the ghosting process. In a multi-method study, participants who had both ghosted and been ghosted in previous romantic relationships (N = 80) provided narratives of their experiences and completed questionnaires. The narrative responses were analyzed by coders and by using LIWC. Ghosters and ghostees used similar overall levels of positively and negatively valenced words to describe their experiences, but ghosters were more likely to express guilt and relief, whereas ghostees were more likely to express sadness and hurt feelings. Ghostees also experienced more of a threat to their fundamental needs - control, self-esteem, belongingness, meaningful existence - than ghosters.

Predicting women's and men's reactions to geographic separation: relational interdependence matters.

Participant sex and a theorized correlate of biological sex, relational interdependence, were tested as relative predictors of the extent to which daters 1) missed and 2) used emotional support to cope when geographically separated from their dating partners. One hundred twenty-four daters completed multiple measures of missing and coping during their colleges' winter breaks. Results from regression analyses indicated that levels of missing and seeking emotional support differed as a function of biological sex. Further, relational interdependence was reliably associated with missing and seeking emotional support in theoretically consistent ways. Mediation analyses highlighted the importance of considering relational interdependence or other individual differences that covary with biological sex when studying close relationship phenomena.

Can you tell that I'm in a relationship? Attachment and relationship visibility on Facebook.

People often attempt to shape others' perceptions of them, but the role of romantic relationships in this process is unknown. The present set of studies investigates relationship visibility, the centrality of relationships in the self-images that people convey to others. We propose that attachment underlies relationship visibility and test this hypothesis across three studies in the context of Facebook. Avoidant individuals showed low desire for relationship visibility, whereas anxious individuals reported high desired visibility (Studies 1 and 2); however, similar motives drove both groups' actual relationship visibility (Study 1). Moreover, both avoidant individuals and their partners were less likely to make their relationships visible (Studies 1 and 3). On a daily basis, when people felt more insecure about their partner's feelings, they tended to make their relationships visible (Study 3). These studies highlight the role of relationships in how people portray themselves to others.

A linear relationship between crystal size and fragment binding time observed crystallographically: implications for fragment library screening using acoustic droplet ejection.

High throughput screening technologies such as acoustic droplet ejection (ADE) greatly increase the rate at which X-ray diffraction data can be acquired from crystals. One promising high throughput screening application of ADE is to rapidly combine protein crystals with fragment libraries. In this approach, each fragment soaks into a protein crystal either directly on data collection media or on a moving conveyor belt which then delivers the crystals to the X-ray beam. By simultaneously handling multiple crystals combined with fragment specimens, these techniques relax the automounter duty-cycle bottleneck that currently prevents optimal exploitation of third generation synchrotrons. Two factors limit the speed and scope of projects that are suitable for fragment screening using techniques such as ADE. Firstly, in applications where the high throughput screening apparatus is located inside the X-ray station (such as the conveyor belt system described above), the speed of data acquisition is limited by the time required for each fragment to soak into its protein crystal. Secondly, in applications where crystals are combined with fragments directly on data acquisition media (including both of the ADE methods described above), the maximum time that fragments have to soak into crystals is limited by evaporative dehydration of the protein crystals during the fragment soak. Here we demonstrate that both of these problems can be minimized by using small crystals, because the soak time required for a fragment hit to attain high occupancy depends approximately linearly on crystal size.

TUNABLE COMPLEMENT ACTIVATION BY PARTICLES WITH VARIABLE SIZE AND Fc DENSITY.

The complement system is an integral innate immune component that is made up of a cascade of enzymatic proteins that, once activated, results in lysis of invading pathogens, opsonization or recruitment of other innate and/or acquired immune responders, or some combination of the three. Due to the importance of the signal amplification and control points present in the cascade, complement is highly sensitive to subtle variations in initiation conditions, including nanoscale changes to molecular spacing. Using Fc-functionalized microparticles and nanoparticles, we find that activation requires a minimum threshold surface concentration of Fc of at least 20% surface coverage. This result indicates that a high surface density Fc is necessary for micro/nanoparticle complement activation through the classical pathway. In addition, the magnitude of the response was dependent on the size of the particle, with larger particles causing decreased activation. We hypothesize that a high density of Fc is needed to efficiently bind and closely appose molecular initiators of the complement cascade, from initiation to terminal complement complex formation. These fundamental studies of the interaction of microparticles and nanoparticles with the immune system suggest design rules for particle size and molecular density that impact immunostimulation through the complement system. Providing a therapeutic agent to modulate the complement response could aid a variety of treatment strategies. Engineered nanoparticles with controlled gaps between molecular activators could lead to new types of immunomodulatory agents.

Pocket-sized psychology studies: exploring daily diary software for palm pilots.

Daily dairies, also known as experience sampling methods (ESM) or everyday experience methods, are a common methodology utilized to provide insight into momentary psychological processes. Traditionally, such studies often have utilized paper-and-pencil surveys administered several times each day over a span of several days or weeks. However, advances in technology now allow these studies to be conducted using palmtop computers (i.e., personal digital assistants; PDAs). Three software packages for running these studies on the Palm operating system were explored and compared on a number of features Specifically, ESP (Experience Sampling Program, by Feldman Barrett & Barrett, 2001), iESP Version 3.2 (Intel Experience Sampling Program, by Intel Research Seattle & the University of Washington Computer Science and Engineering Department), and PMAT Version 2.0 (Purdue Momentary Assessment Tool, by the Military Family Research Institute at Purdue University) were examined, with their key features compared. These advances in software for running diary studies include a number of features that provide researchers with methods and information previously unavailable in diary studies and may expand the range of possibilities in diary study designs.