Histological and transcriptomic effects of 17α-methyltestosterone on zebrafish gonad development.

BACKGROUND: Sex hormones play important roles in teleost ovarian and testicular development. In zebrafish, ovarian differentiation appears to be dictated by an oocyte-derived signal via Cyp19a1a aromatase-mediated estrogen production. Androgens and aromatase inhibitors can induce female-to-male sex reversal, however, the mechanisms underlying gonadal masculinisation are poorly understood. We used histological analyses together with RNA sequencing to characterise zebrafish gonadal transcriptomes and investigate the effects of 17α-methyltestosterone on gonadal differentiation. RESULTS: At a morphological level, 17α-methyltestosterone (MT) masculinised gonads and accelerated spermatogenesis, and these changes were paralleled in masculinisation and de-feminisation of gonadal transcriptomes. MT treatment upregulated expression of genes involved in male sex determination and differentiation (amh, dmrt1, gsdf and wt1a) and those involved in 11-oxygenated androgen production (cyp11c1 and hsd11b2). It also repressed expression of ovarian development and folliculogenesis genes (bmp15, gdf9, figla, zp2.1 and zp3b). Furthermore, MT treatment altered epigenetic modification of histones in zebrafish gonads. Contrary to expectations, higher levels of cyp19a1a or foxl2 expression in control ovaries compared to MT-treated testes and control testes were not statistically significant during early gonad development (40 dpf). CONCLUSION: Our study suggests that both androgen production and aromatase inhibition are important for androgen-induced gonadal masculinisation and natural testicular differentiation in zebrafish.

A molecular extraction process for vanadium based on tandem selective complexation and precipitation.

Recycling vanadium from alternative sources is essential due to its expanding demand, depletion in natural sources, and environmental issues with terrestrial mining. Here, we present a complexation-precipitation method to selectively recover pentavalent vanadium ions, V(V), from complex metal ion mixtures, using an acid-stable metal binding agent, the cyclic imidedioxime, naphthalimidedioxime (H2CIDIII). H2CIDIII showed high extraction capacity and fast binding towards V(V) with crystal structures showing a 1:1 M:L dimer, [V2(O)3(C12H6N3O2)2]2-, 1, and 1:2 M:L non-oxido, [V(C12H6N3O2)2] ̶ complex, 2. Complexation selectivity studies showed only 1 and 2 were anionic, allowing facile separation of the V(V) complexes by pH-controlled precipitation, removing the need for solid support. The tandem complexation-precipitation technique achieved high recovery selectivity for V(V) with a selectivity coefficient above 3 × 105 from synthetic mixed metal solutions and real oil sand tailings. Zebrafish toxicity assay confirmed the non-toxicity of 1 and 2, highlighting H2CIDIII's potential for practical and large-scale V(V) recovery.

Facile fabrication of a 2D/2D CoFe-LDH/g-C3N4 nanocomposite with enhanced photocatalytic tetracycline degradation.

The widespread use of tetracycline (TC) in medicine and agriculture has caused severe pollution problems in the environment. In this work, a nanocomposite comprising of CoFe-layered double hydroxides grown on graphitic carbon nitride nanosheets (CoFe-LDH/g-C3N4) with a notable two-dimensional/two-dimensional (2D/2D) heterostructure was synthesized through a facile co-precipitation method. The CoFe-LDH/g-C3N4 nanocomposite displayed significantly improved visible-light-driven photocatalytic activity towards TC degradation, compared to pristine g-C3N4 and CoFe-LDH alone. The enhanced activation efficiency was a result of intimate interfacial contact, enlarged the surface area, broadened visible-light absorbance, and enhanced photogenerated electron transfer. The scavenging experiments showed that holes (h+) and superoxide radical anions (‧O2-) played a crucial role in TC degradation. Factors including the type of TCs, initial concentration of TC, presence of ions, and the type of water matrix were investigated to evaluate the practical feasibility of the nanocomposites for TC removal from antibiotics-contaminated water. The repeated tests showed that the nanocomposites possessed good stability and recyclability. This study demonstrated the feasibility of achieving photocatalytic activity enhancement of g-C3N4 through the formation of a 2D-2D heterostructure between LDHs and g-C3N4.

A Mini Review on Persulfate Activation by Sustainable Biochar for the Removal of Antibiotics.

Antibiotic contamination in water bodies poses ecological risks to aquatic organisms and humans and is a global environmental issue. Persulfate-based advanced oxidation processes (PS-AOPs) are efficient for the removal of antibiotics. Sustainable biochar materials have emerged as potential candidates as persulfates (Peroxymonosulfate (PMS) and Peroxydisulfate (PDS)) activation catalysts to degrade antibiotics. In this review, the feasibility of pristine biochar and modified biochar (non-metal heteroatom-doped biochar and metal-loaded biochar) for the removal of antibiotics in PS-AOPs is evaluated through a critical analysis of recent research. The removal performances of biochar materials, the underlying mechanisms, and active sites involved in the reactions are studied. Lastly, sustainability considerations for future biochar research, including Sustainable Development Goals, technical feasibility, toxicity assessment, economic and life cycle assessment, are discussed to promote the large-scale application of biochar/PS technology. This is in line with the global trends in ensuring sustainable production.

Constructing novel graphitic carbon nitride-based nanocomposites - From the perspective of material dimensions and interfacial characteristics.

Two-dimensional (2D) graphitic carbon nitride (g-C3N4), a fascinating metal-free conjugated polymer, has garnered immense interest in the fields of solar power generation and environmental remediation. The construction of g-C3N4-based nanocomposites with materials of various dimensions can further improve their photocatalytic activities by surface area enlargement, bandgap tuning, heterojunction formation, etc. In this paper, we comprehensively reviewed the design, synthesis, and functionalities of g-C3N4-based nanocomposites based on their applications in hydrogen evolution, CO2 reduction, and pollutants removal. We provided detailed analyses on the integration of 2D g-C3N4 with zero-, one-, two-, and three-dimensional materials with a focus on their interfacial characteristics and functional improvement. This review aims to stimulate fresh ideas on the interfacial engineering of g-C3N4-based nanocomposites to broaden their future applications.

Enhanced visible light-driven photodegradation of tetracycline by salicylic acid-modified graphitic carbon nitride and toxicity assessment.

The tetracycline (TC) in water has led to serious concern. Graphitic carbon nitride (g-C3N4) photocatalysts were produced via copolymerization of mono-benzene ring-mediated precursors (urea, melamine, and dicyandiamide) involving salicylic acid (SA) for TC degradation. The SA-modified g-C3N4 samples showed improved visible light absorbance, transfer and separation of photogenerated electrons, and prospective photocatalytic application in TC degradation. As a result, the optimal SA-modified g-C3N4 (2 wt% of SA) using urea (CNU-SA-2) showed 2 times higher TC degradation than that of pristine g-C3N4. The process of TC degradation was evaluated by the reduction of antibacterial activity and extensively studied by varying the types of TC, initial pH values, co-existing anions, and natural organic materials. In addition, the catalyst could be reused for at least four cycles, indicating good reusability. The main active species were revealed to be h+ and ·O2- by scavenging experiments and electron spin resonance. The CNU-SA-2 photocatalyst and TC intermediates during degradation had no adverse impact on zebrafish embryos. This work could provide a design strategy and a perspective on the practical application of g-C3N4-based photocatalysts for the treatment of wastewater containing antibiotics.