Insights into the biogeochemical transformation, environmental impacts and biochar-based soil decontamination of antimony.

Every year, a significant amount of antimony (Sb) enters the environment from natural and anthropogenic sources like mining, smelting, industrial operations, ore processing, vehicle emissions, shooting activities, and coal power plants. Humans, plants, animals, and aquatic life are heavily exposed to hazardous Sb or antimonide by either direct consumption or indirect exposure to Sb in the environment. This review summarizes the current knowledge about Sb global occurrence, its fate, distribution, speciation, associated health hazards, and advanced biochar composites studies used for the remediation of soil contaminated with Sb to lessen Sb bioavailability and toxicity in soil. Anionic metal(loid) like Sb in the soil is significantly immobilized by pristine biochar and its composites, reducing their bioavailability. However, a comprehensive review of the impacts of biochar-based composites on soil Sb remediation is needed. Therefore, the current review focuses on (1) the fundamental aspects of Sb global occurrence, global soil Sb contamination, its transformation in soil, and associated health hazards, (2) the role of different biochar-based composites in the immobilization of Sb from soil to increase biochar applicability toward Sb decontamination. The review aids in developing advanced, efficient, and effective engineered biochar composites for Sb remediation by evaluating novel materials and techniques and through sustainable management of Sb-contaminated soil, ultimately reducing its environmental and health risks.

Distribution, source identification, water quality, and risk assessment of trace elements in the surface-groundwater-sediments multifunctional system in Guohe River Basin.

Within the Huaihe River Basin, Guohe River, as its second-largest tributary, serves as a critical water supply source. Recent industrial and agricultural advancements have led to increased trace element contamination, adversely impacting the water quality within Guohe River Basin. Therefore, this study aimed to investigate the distribution characteristics, sources, water quality and risk assessment of trace elements in the surface water, groundwater, and sediments across the basin. The results showed that the spatial distribution of trace elements in the surface water and groundwater of Guohe River Basin was that most of the high concentrations appeared in Qiaocheng District of Bozhou City, the mean concentration of Fe in Guohe River sediments was the highest, the mean concentration of Sb was the lowest. The PMF source analysis results showed that the main source of trace elements in Guohe River Basin was natural geological processes, followed by human activities. The sodium adsorption ratio (SAR) indicated that the surface water samples of Guohe River in two seasons had high sodium and salinity hazards. The water quality index (WQI) showed that surface water and groundwater samples in the northwestern of Guohe River Basin had poor water quality. The results of the risk assessment showed that As and Mn posed great ecological risks to surface water and groundwater, respectively, and that F- was the pollutant with the most potential health risk hazard in the basin. The Geo-accumulation index (Igeo) results showed that Cd, Se and As should be taken seriously as the main contaminants of the sediments in Guohe River Basin. KEYWARDS: Trace elements; Source analysis; Sodium adsorption ratio; Water quality index; Risk assessment; Geo-accumulation index.

Speciation Characterization and Environmental Stability of Arsenic in Arsenic-Containing Copper Slag Tailing.

The increasing presence of arsenic-containing impurities within Cu ores can adversely affect the smelting process and aggravate the environmental impact of slag tailing. This study investigates the geochemical, mineralogical, and chemical speciation characteristics to better understand the association and environmental stability of metal(loid)s in copper slag tailing. The results indicate that the predominant chemical compositions of the selected slag tailing are Fe2O3 (54.8%) and SiO2 (28.1%). These tailings exhibit potential for multi-elemental contamination due to elevated concentrations of environmentally sensitive elements. Mineral phases identified within the slag tailings include silicate (fayalite), oxides (magnetite and hematite), and sulfides (galena, sphalerite, arsenopyrite, and chalcopyrite). The consistent presence of silicate, iron, arsenic, and oxygen in the elemental distribution suggests the existence of arsenic within silicate minerals in the form of Si-Fe-As-O phases. Additionally, arsenic shows association with sulfide minerals and oxides. The percentages of arsenite (As(III)) and arsenate (As(V)) within the selected slag tailings are 59.4% and 40.6%, respectively. While the slag tailings are deemed non-hazardous due to the minimal amounts of toxic elements in leachates, proper disposal measures should be taken due to the elevated carbonate-bound levels of As and Cu present in these tailings.

Study on the solidification performance and mechanism of heavy metals by sludge/biomass ash ceramsites, biochar and biomass ash.

Industrial by-products are stored in large quantities in the open, leading to wasted resources and environmental pollution, and the natural environment is similarly faced with phosphate depletion and serious water and soil pollution. This study uses these by-products to produce a new sludge/biomass ash ceramsite that will be used to adsorb nitrogen and phosphorus from wastewater, and solidify heavy metals in the soil while releasing Olsen P. The sludge/biomass ash ceramsites are made using sewage sludge and biomass ash in a certain ratio calcined at high temperatures and modified for the adsorption of nitrogen and phosphorus from wastewater. Sludge/biomass ash ceramsites before and after phosphorus adsorption, biochar and biomass ash were compared to analyze their heavy metal adsorption capacity and potential as phosphate fertilizer. After phosphorus adsorption, the sludge/biomass ash ceramsites released effective phosphorus steadily and rapidly in the soil, with a greater initial release than biochar and biomass ash, and the ceramsites were in a granular form that could be easily recycled. Biochar and biomass residue, due to their surface functional groups, are better at solidifying heavy metals than sludge/biomass ash ceramsites. Biochar, biomass ash and sludge/biomass ash ceramsites significantly reduced the concentrations of Cd, Cu, Pb and Zn in the soil. Correlation analysis demonstrated that there was a synergistic relationship between the increase in soil Olsen P content and the change in pH, with the increase in soil Olsen P content and the increase in pH contributing to heavy metal solidification.

Synthesis of ordered mesoporous silica from biomass ash and its application in CO2 adsorption.

It is possible to achieve high-value utilization of solid wastes and lower the cost of mesoporous silica synthesis by synthesizing mesoporous silica from solid wastes. In this study, silica was extracted using the alkali fusion method using biomass ash as the starting material. Biomass ash based mesoporous silica was successfully prepared by hydrothermal method with silicon extract solution as silicon source. The optimum conditions for preparation were determined as follows: addition of cetyltrimethylammonium bromide was 0.45g, hydrothermal temperature was 120 °C, hydrothermal time was 24h. The prepared mesoporous silicon was systematically characterized, and the results showed that high surface area (495 m2/g) and ordered pore structure appeared in the synthesized mesoporous silica materials. The synthesized mesoporous silica showed excellent CO2 adsorption performance (0.749 mmol/g) at 25 °C and 1 bar. According to the calculation of adsorption isotherm and thermodynamics, non-linear Freundlich model can fit the adsorption isotherm better and the adsorption heat of mesoporous silica is less than 20 kJ/mol, which belongs to physical adsorption. After five cycles of CO2 adsorption, the adsorption property was still above 90%, and the CO2/N2 adsorption selectivity reached 396.6, showing good regeneration performance and adsorption selectivity. This research can provide a new possibility for the high-value exploitation of biomass ash and reducing the cost of synthetic mesoporous silica.

Role of biochar-based free radicals in immobilization and speciation of metals in the contaminated soil-plant environment.

The structure of biochar produced at various pyrolysis temperatures influences metal geochemical behavior. Here, the impact of wheat straw-derived biochar (300, 500, and 700 °C) on the immobilization and transformation of metals in the contaminated soil-plant system was assessed. The findings of the sequential extraction revealed that biochar additives had a substantial influence on the speciation of Cr, Ni, Pb, and Zn in the contaminated soil. The lowest F1 (exchangeable and soluble fraction) + F2 (carbonate fraction) accounted for Cr (44%) in WB-300, Ni (43.87%) in WB-500, Pb (43.79%), and Zn (49.78%) in WB-700 with applied amendments of their total amounts. The characterization results indicated that high pyrolysis temperatures (300-700 °C) increased the carbon-containing groups with the potential to adsorb metals from the soil-plant environment. The bioconcentration and translocation factors (BCF and TF) were less than 1, indicating that metal concentration was restricted to maize roots and translocation to shoots. Reactive oxygen species (ROS) intracellularly influence metal interactions with plants. Electron paramagnetic resonance (EPR) was performed to determine hydroxyl radical generation (•OH) in plant segments to assess the dominance of free radicals (FRs). Consequently, the formation of •OH significantly depends on the pyrolysis temperature and the interaction with a contaminated soil-plant environment. Thus, metal transformation can be effectively decreased in the soil-plant environment by applying WB amendments.

Influence of biochar produced from negative pressure-induced carbonization on transformation of potentially toxic metal(loid)s concerning plant physiological characteristics in industrially contaminated soil.

Soil contamination and its subsequent impact on the food chain is a pressing challenge in the present day. The application of biochar has demonstrated a significant and positive effect on soil health, thereby enhancing plant growth and development. However, the application of biochar (BC) produced from negative pressure-induced carbonization to mitigate metal(loid) contamination is a new strategy that has been studied in current research. Results depicted that the application of biochar derived from the negative pressure carbonization (vacuum-assisted biochar (VBC) has a significant (p ≤ 0.05) positive impact on plant growth and physiological characteristics by influencing immobilization and speciation of metal(loid) in the soil system. Moreover, the interactive effect of VBC on physiological characteristics (photosynthesis, gas exchange, and chlorophyll contents) and antioxidant activities of maize (Zea mays L.) was significantly (p ≤ 0.05) positive by confining the translocation and movement of metal(loid)s to the aerial part of the maize plant. X-ray diffraction (XRD) provided information on the structural and chemical changes induced by the VBC-500 °C explaining metal(loid) adsorption onto mineral surfaces and complexation that can affect their mobility, availability, and toxicity in the contaminated soil. Fourier transform infrared spectroscopy (FTIR) further provided a more detailed understanding of the metal(loid)s and biochar complexation mechanisms influenced by VBC-based functional groups -OH, C-Hn, -COOH, CO, C-O-C, CC, C-O, C-H, OH, and C-C in the binding process. These results suggest that the application of biochar prepared at 500 °C under negative pressure-induced carbonization conditions to the soil is the most efficient way to reduce the uptake and transfer of metal(loid)s by influencing their mobility and availability in the soil-plant system.

Characterization of the Physical Chemistry Properties of Iron-Tailing-Based Ceramsite.

In order to deal with the problems of resource waste and environmental pollution caused by solid waste, iron tailings (mainly SiO2, Al2O3 and Fe2O3) were used as the main raw material to create a type of lightweight and high-strength ceramsite. Iron tailings, dolomite (industrial grade, purity 98%) and a small amount of clay were combined in a N2 atmosphere at 1150 °C. X-ray fluorescence spectrometry (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and a themogravimetric analysis (TGA) were performed and the specific surface area was analyzed to determine the strength and adsorption of the ceramsite. The results of the XRF showed that SiO2, CaO and Al2O3 were the main components of the ceramsite, with MgO and Fe2O3 also included. The results of the XRD and SEM-EDS showed that the ceramsite contained several kinds of minerals and was mainly composed of akermanite, gehlenite and diopside, and that the morphology of the internal structure of the ceramsite was mainly massive and contained a small number of particles. The ceramsite could be used in engineering practice to improve the mechanical properties of materials and meet the requirements of actual engineering for the strength of materials. The results of the specific surface area analysis showed that the inner structure of the ceramsite was compact and that there were no large voids. The voids were mainly medium and large, with a high stability and strong adsorption ability. The TGA results showed that the quality of the ceramsite samples will continue to increase within a certain range. According to the XRD experimental results and experimental conditions, it was speculated that in the part of the ore phase containing Al, Mg or Ca in the ceramsite, the elements underwent relatively complex chemical reactions with each other, resulting in the formation of an ore phase with a higher molecular weight. This research provides the basis of characterization and analysis for the preparation of high-adsorption ceramsite from iron tailings and promotes the high-value utilization of iron tailings for waste pollution control.

Spatial-temporal distribution, occurrence, water quality, and risk assessment of trace elements in ten rivers surrounding Chaohu Lake in China.

As one of the five great lakes in China, the Chaohu Lake Basin is the main water source for regional economic and ecological development in Hefei city and is considered a source of drinking water. The spatial-temporal distributions and occurrence of soluble trace elements were studied in the surrounding ten rivers in the Chaohu Lake Basin as well as water quality and risk assessment during the normal and wet seasons. Principal component analysis (PCA) showed that the main source of river pollution during the two seasons was the mining industry. High values of most elements were found in the northwestern rivers in the two seasons. The temporal changes in the elements showed that the distributions of As, Mn, Cd, and Cu in the two seasons were very different, but the trends of Ni, Co, and V were basically the same. The sodium adsorption ratio (SAR) showed that almost all river samples needed to be treated before irrigation, and the water quality index (WQI) showed that most samples were of excellent water quality for drinking. The ecological risk assessment results showed that the risks in the two seasons were all slight. The results of the health risk evaluation suggested that no noncarcinogenic risks were found in the normal season and that the carcinogenic risks from Cr and As reached their highest levels in the normal and wet seasons, respectively. This research can provide vital data for rational water control and water quality conservation, offer a scientific basis for ecological environment safety, and offer a reference for carcinogenic and noncarcinogenic health risks to regional residents.

Comparison of pollution status and source apportionment for PCBs and OCPs of indoor dust from an industrial city.

In this study, the pollution status of polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCPs) was investigated in indoor and outdoor dust from three different functional areas of Hefei, China. The relationship between the concentrations of PCBs and OCPs and different influencing factors in dwellings was studied. The results showed that the concentrations of PCBs and OCPs were higher in samples from dwellings with higher smoking frequency, lower cleaning frequency, higher floors and smaller household size. The results of Spearman's correlation coefficient analysis indicated that PCBs and OCPs were not consistently associated with each other, while sources of low-chlorinated PCBs and high-chlorinated PCBs were different. Scanning electron microscopy (SEM) shows the shape of indoor dust was a mixture of blocky, flocculated, spherical structures, and irregular shapes. The results of principal component analysis (PCA) and positive matrix factorization model (PMF) showed that the PCBs and OCPs of indoor dust came from both indoor and outdoor sources between local and regional transport. Carbon (δ13C) and Nitrogen (δ15N) stable isotope results indicate or show that the indoor dust (δ13C: - 24.37‰, δ15N: 6.88‰) and outdoor dust (δ13C: - 12.65‰, δ15N: 2.558‰) is derived from fossil fuel, coal combustion, road dust, fly ash, C4 biomass and soil. Potential source contribution factor (PSCF) and concentration weighted-trajectory analysis suggest that sources of pollutants were local and regional transport from surrounding provinces and marine emissions. The average daily dose (adult: 8.20E-04, children: 2.37E-03) of pollutants and the carcinogenic risks (adult: 1.23E-02, children: 2.65E-02) were relatively greater for children than adults. This study demonstrates the utility of SEM to characterize indoor dust morphology while combining PMF, PSCF, and stable isotope methods in identifying indoor PCBs and OCPs sources and regions.

Chromium in Chinese coals: geochemistry and environmental impacts associated with coal-fired power plants.

Chromium (Cr), one of the prime hazardous trace elements in coals, may engender adverse effects on eco-environment and threaten human health during utilization of coal. Based on the samples obtained in our laboratory and published literature, the abundance and modes of occurrence of Cr in Chinese coals, and the environmental impacts associated with coal-fired power plants (CFPPs) were elucidated in this study. With a total of 1397 sets of data, the mean concentration of Cr in Chinese coals was calculated as 21.33 µg/g by the "reserve-concentration" weighted calculation method. Spatially, the average Cr contents increased gradually from North China to South China. Temporally, coals from T3, E-N and P2 were relatively enriched in Cr compared to the other geological time. The Cr concentration in coal varied with different coal ranks. The geological factors accounted for Cr enrichment in coals could be divided into the primary, secondary and epigenetic processes. Higher percentages of organically Cr occurred in low-rank coals, while inorganically associated Cr was mainly found in clay minerals. After coal combustion, most of Cr was enriched in solid wastes (e.g., fly ash and bottom ash). The leaching of Cr from solid wastes in the rainy season (especially acid rain) needs to be a concern for CFPPs. It was estimated that the atmospheric emission of Cr from CFPPs increased annually from 2015 to 2019 and reached approximately 159 tons in 2019.

Material flow analysis and characteristics of multiple-source organic solid wastes from a perspective of ecological network analysis: A case study in Hefei, China.

The numerous resources are consumed and a large number of multiple-source organic solid wastes are discharged during urban metabolic, which put pressures to environment. This study combined material flow analysis and ecological network analysis to comprehensively analyse the metabolism of multiple-source organic solid waste in Hefei. Analysing current metabolic characteristics of urban multiple-source organic solid waste and relationships between the components of metabolic system can help urban environmental management. The internal material flows of the system were analysed by a metabolic network containing 5 nodes and 18 pathways, which revealed metabolic characteristics of multiple-source organic solid waste in Hefei. The study showed that the metabolic system of multiple-source organic solid waste was relatively stable though distribution of internal flows changed in 8 years. The metabolic environment and agricultural sector covered a relatively large weight in this model. The control and exploitation relationships dominated the metabolic system. The urban metabolic mechanism reflected in this research can improve efficiency of urban metabolic resource, optimize waste management and provide data support for sustainable urban development. This study provided scientific guidance for metabolic research in Hefei and similar cities in China's rapid economic development.

Green synthesis of ceramsite from industrial wastes and its application in selective adsorption: Performance and mechanism.

The increasing requirement and consumption of coal has resulted in a large accumulation of coal gangue. The reuse and recycling of coal gangue have become a high priority for sustainable development. A sustainable and efficient ceramsite adsorbent was prepared for copper ions adsorption by using coal gangue, coal fly ash, and copper slag as the main materials. The appropriate performance of the ceramsite could be obtained at a mixture of coal gangue, coal fly ash, and copper slag at a weight ratio of 3:4:1. The optimal sintering temperature and time were 1050 °C and 20 min, respectively. The main crystalline phases of ceramsite were quartz, mullite, and anorthite. Many micropores are connecting the interior on the surface of ceramsite under scanning electron microscope. The maximum copper ions adsorption capacity reached up to 20.6 mg/g at 303 K when pH and time were 5 and 1440 min, respectively. The adsorption kinetics and isotherm could be described by the pseudo-second-order model and Freundlich model, respectively. The adsorption mechanisms of Cu2+ with ceramsite were attributed to Cu(OH)2 precipitation formed on the alkaline surface of ceramsite and complexation reactions occurred between the O-containing groups (including C-O, Fe-O, and Si-O) from ceramsite and Cu2+. The prepared ceramsite may be also applied to other heavy metal wastewater treatments.

Serum non-coding RNAs for diagnosis and stage of liver fibrosis.

BACKGROUND: All chronic liver diseases could lead to liver fibrosis. Accurate diagnosis and stage of fibrosis were important for the medical determination, management, and therapy. Liver biopsy was considered to be the gold criteria of fibrosis diagnosis. However, liver biopsy was an invasive method with some drawbacks. Non-invasive tests for liver fibrosis included radiologic method and serum-based test. Radiologic examination was influenced by obesity, cost, and availability. Serum-based test was widely used in the screening and diagnostic of liver fibrosis. However, the accuracy was still needed to be improved. METHODS: Recent studies showed serum non-coding RNAs: microRNA, long non-coding RNA(lncRNA), and circular RNA(circRNA), which have the potentiality to be non-invasive markers for liver fibrosis. The recent progress was summarized in this review. RESULTS: These studies showed serum non-coding RNAs exerted a good diagnostic performance for liver fibrosis. A panel that included several non-coding RNAs could increase the accuracy of single marker. CONCLUSIONS: Serum microRNAs, lncRNAs, and circRNAs could be potential non-invasive markers for diagnosis and stage of liver fibrosis. More high-quality clinical study is needed for further research.

Morpho-chemical characterization and source apportionment of potentially toxic metal(oid)s from school dust of second largest populous city of Pakistan.

Interior settled dust is one of the greatest threats of potentially toxic metal(oid)s (PTMs) exposure to the children, especially in the school environment. Therefore, it is more worthy of having in-depth knowledge of compositional characteristics of school dust. Forty schools were selected of Lahore city for dust sampling. The school dust was analyzed to determine the PTMs (As, Cd, Cr, Cu, Ge, Mo, Ni, Pb, Sb, Sn, Sr, V, and Zn) concentrations using ICP-MS. The morphological characteristics, PTMs speciation, and mineralogy of school dust were examined using SEM with EDS, XPS, and XRD, respectively. Moreover, the geo-accumulation index (Igeo), potential ecological risk index (PERI), and multivariate statistical analysis were employed to assess the pollution levels, ecological risk, and source identification of PTMs, respectively. The Igeo indicated a heavily-extreme pollution level of Cd (Igeo = 4.92), moderate-heavy pollution of Zn (Igeo = 3.22), and Pb (Igeo = 2.78), and slight-moderate pollution of Cr (Igeo = 1.62), and Cu (Igeo = 1.53). The ecological risk has been found extremely high for Cd and moderately high for Pb and As, while potential ecological risk found extremely high posed by cumulatively all selected PTMs. Multivariate statistical analysis showed that sources of PTMs comprise of natural processes as well as several anthropogenic processes like vehicular emissions, agricultural and industrial activities. The SEM, XRD, and XPS analyses demonstrated the presence of airborne particles and PTMs containing minerals with several toxic chemical species in school dust. This study can help to develop strategies to reduce school indoor pollution and hence to establish an eco-friendly learning environment for children.

Study of the micromorphology and health risks of arsenic in copper smelting slag tailings for safe resource utilization.

Slag tailings are produced by "cooling-grinding-ball milling-flotation" and other processes of slag, while slag is produced by the flash smelting of the original ore. The utilization and environmental hazards of arsenic in slag tailings have become a focus of attention. This study on slag tailings reveals the presence of arsenic in copper smelting tailings from the mineralogy and leaching perspectives, and the noncarcinogenic and carcinogenic risks of arsenic to the human body were assessed by using the USEPA health risk model. The surface particles of the slag tailings were unevenly dispersed, and the mineral crystals were relatively complete. A small amount of secondary minerals had grown on the mineral surface. Most of the fine particles adhered to the surface of the main mineral to form inclusions. The mineral composition of the slag tailings was dominated by maghemite (Fe3O4) and fayalite (Fe2SiO4), and the arsenic-bearing minerals were unevenly distributed, where As (Ⅴ) fine particles were embedded in maghemite, amorphous phase and fayalite. There was a large amount of residual arsenic in the slag tailing particles, and the leaching content of arsenic in the toxicity leaching procedure was always lower than the limit of 5 mg/L. The health risk to the exposed population was evaluated by the USEPA health risk model. Since the exposed population in the industrial land is mainly adults, it is determined that the tailings will not cause harm to children's health. In this evaluation, the exposure duration (length of service of the workers) of 30 years, exposure frequency of 314 d/y and body weight of 60 kg (average weight of the workers) were taken as the parameters of three exposure pathways: hand-oral ingestion, respiratory system inhalation and skin contact. Therefore, longer activity time of the workers in the tailing workshop corresponds to a higher HI (hazard index). Although the arsenic in the slag tailings had a certain degree of bioavailability, it was not sufficient to adversely affect human health.

Synthesis of Calcium Silicate Hydrate from Coal Gangue for Cr(VI) and Cu(II) Removal from Aqueous Solution.

Both the accumulation of coal gangue and potentially toxic elements in aqueous solution have caused biological damage to the surrounding ecosystem of the Huainan coal mining field. In this study, coal gangue was used to synthesize calcium silicate hydrate (C-S-H) to remove Cr(VI) and Cu(II)from aqueous solutions and aqueous solution. The optimum parameters for C-S-H synthesis were 700 °C for 1 h and a Ca/Si molar ratio of 1.0. Quantitative sorption analysis was done at variable temperature, C-S-H dosages, solution pH, initial concentrations of metals, and reaction time. The solution pH was precisely controlled by a pH meter. The adsorption temperature was controlled by a thermostatic gas bath oscillator. The error of solution temperature was controlled at ± 0.3, compared with the adsorption temperature. For Cr(VI) and Cu(II), the optimum initial concentration, temperature, and reaction time were 200 mg/L, 40 °C and 90 min, pH 2 and 0.1 g C-S-H for Cr(VI), pH 6 and 0.07 g C-S-H for Cu(II), respectively. The maximum adsorption capacities of Cr(VI) and Cu(II) were 68.03 and 70.42 mg·g-1, respectively. Furthermore, the concentrations of Cu(II) and Cr(VI) in aqueous solution could meet the surface water quality standards in China. The adsorption mechanism of Cu(II) and Cr(VI) onto C-S-H were reduction, electrostatic interaction, chelation interaction, and surface complexation. It was found that C-S-H is an environmentally friendly adsorbent for effective removal of metals from aqueous solution through different mechanisms.

Geochemical Anomaly Characteristics of Cd in Soils around Abandoned Lime Mines: Evidence from Multiple Technical Methods.

Lime mines are a potential source of pollution, and the surrounding soil environment is generally at threat, especially in abandoned lime mines. This paper focuses on the study area in eastern Anhui, attempting to analyze whether Cd enrichment is related to abandoned mines. On the basis of geological investigation, this study systematically used XRD, XRF, GTS and universal Kriging interpolation to determine the distribution law of Cd in the study area, and evaluated the potential ecological risk of Cd. The results showed that the main mineral types of soil samples of red clastic rock soil parent material (RdcPm) and soil samples of carbonate soil parent material (CPm) were not completely the same. Correlation analysis showed that CaO, MgO and Cd were positively correlated with the CPm. Human activities led to the accumulation of Cd in the study area. High Cd was mainly concentrated in the northwest of the study area, which was correlated with abandoned mines and soil parent materials. The study area was dominated by slight potential risks, although some areas had medium potential risks and high potential risks. All potential high risks were in the CPm field. This study provides a scientific basis for the comprehensive utilization and development planning of soil in the study area.

Geochemical fractionation and spectroscopic fingerprinting for evaluation of the environmental transformation of potentially toxic metal(oid)s in surface-subsurface soils.

The contamination of soil by toxic metal(oid)s has emerged as a major concern worldwide, particularly in developing countries. A metals behavior in the soil environment is influenced by organic matter, mineral phases, and oxidation states in which a particular metal exists. However, the spectroscopic evidence of metal(oid)s interactions in soil with organic matter and mineral phases can induce an extensive understanding. The surface and sub-surface soils (0-50 cm) from four sites of upper Indus basin, Pakistan, were collected and analyzed by using FTIR (Fourier-transform infrared spectroscopy), XRD (X-ray diffraction) and XPS (X-ray photoelectron spectroscopy) in addition to ICP-MS (inductively coupled plasma mass spectrometry) and geochemical fractionation. Geochemical fractionation of metal(oid)s indicated that As, Cu, Ni, Pb, and Zn were mostly found in the potentially bioavailable fractions. However, an increase in the residual fraction was observed from top to bottom. The absorption bands of FTIR spectra were divided into three spectral regions 700-400, 1700-800, and 3700-2800 cm-1. The soil was found rich in organic matter and capable of retaining metals as abundant peaks were observed in the mid-infrared region. The mineralogical analysis of soil samples testified silicon oxides and zeolite as major mineral phases. The XPS spectra showed broad peaks of As(III), As2O3, As4S4, PbO2, and PbCo3. The study concludes that the source identification of metal(oid)s in the upper Indus is crucial to find out the particular source of contamination in the soil.

Tanshinone I regulates autophagic signaling via the activation of AMP-activated protein kinase in cancer cells.

Tanshinone I, one of the components of Salvia miltiorrhiza Bunge, exhibits anti-tumor ability and induces autophagy. But the mechanisms are not fully understood. This study aims to investigate whether AMP-activated protein kinase dependent pathway is involved in the autophagic signaling regulation and its relationship with tumor suppression. Breast cancer cells (MDA-MB-231, MCF-7) and hepatocellular carcinoma cells (HepG2) were treated with Tanshinone I or vehicle. Acridine orange dyeing and transmission electron microscopy were employed to evaluate autophagic cells. MTT and Cell Counting Kit-8 assays were used to detect the effect of Tanshinone I combined with autophagy inhibitors on cell proliferation. Western blot was used to detect the expression levels of Beclin1 and LC3-I/II, as well as the phosphorylation of AMPKα and ULK1. Our results showed that Tanshinone I suppressed proliferation of HepG2, MDA-MB-231 and MCF-7 cancer cell lines. LC3-II and P62 were induced by Tanshinone I in all three cancer cell lines. But autophagic flux analysis showed that Tanshinone I treatment induced autophagy only in MDA-MB-231, which was also proved by transmission electron microscopy. Tanshinone I upregulated the phosphorylation of AMPKα and its downstream ULK1. AMP-activated protein kinase inhibitor compound C attenuated Beclin 1 and LC3-II expression induced by Tanshinone I in HepG2. In MDA-MB-231, compound C surprisingly induced LC3-II upregulation which is independent of AMPKα activation. Under this circumstance, treatment of Tanshinone I combined with compound C significantly inhibited MDA-MB-231 proliferation, compared with Tanshinone I treatment alone. This study demonstrates that Tanshinone I could induce cancer cell death and regulate autophagy signaling in breast cancer and hepatic carcinoma cells. Activation of AMPKα was found to be involved in autophagic signaling regulation by Tanshinone I.