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In this study, phosphoric acid activation was employed to synthesize nitrogen-doped mesoporous activated carbon (designated as MR1) from Lentinus edodes (shiitake mushroom) residue, while aiming to efficiently remove acetaminophen (APAP), carbamazepine (CBZ), and metronidazole (MNZ) from aqueous solutions. We characterized the physicochemical properties of the produced adsorbents using scanning electron microscopy (SEM), nitrogen adsorption isotherms, and X-ray photoelectron spectroscopy (XPS). MR1, MR2, and MR3 were prepared using phosphoric acid impregnation ratios of 1, 2, and 3 mL/g, respectively. Notably, MR1 exhibited a significant mesoporous structure with a volume of 0.825 cm3/g and a quaternary nitrogen content of 2.6%. This endowed MR1 with a high adsorption capacity for APAP, CBZ, and MNZ, positioning it as a promising candidate for water purification applications. The adsorption behavior of the contaminants followed the Freundlich isotherm model, suggesting a multilayer adsorption process. Notably, MR1 showed excellent durability and recyclability, maintaining 95% of its initial adsorption efficiency after five regeneration cycles and indicating its potential for sustainable use in water treatment processes.
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Beyond its economic value, copper (Cu) serves as a valuable tracer of deep magmatic processes due to its close relationship with magmatic sulfide evolution and sensitivity to oxygen fugacity (fO2). However, determining Cu's oxidation state (+ 1 or + 2) in silicate melts, crucial for interpreting its behavior and reconstructing fO2 in the Earth's interior, has long been a challenge. This study utilizes X-ray Absorption Near Edge Structure spectroscopy to investigate the Cu oxidation state in hydrous mafic silicate melts equilibrated under diverse fO2 (- 1.8 to 3.1 log units relative to the Fayalite-Magnetite-Quartz buffer), temperature (1150-1300 °C), and pressure (1.0-2.5 GPa) conditions. Our results reveal that Cu predominantly exists as Cu+ across all fO2 conditions, with a minor Cu2+ component. This dominance of Cu+ persists even in relatively oxidized melts, highlighting its limited sensitivity to fO2 under upper mantle conditions. This significantly constrains the utility of Cu as an oxybarometer in hydrous silicate melts in the deep Earth. However, our findings suggest that Cu isotopes primarily reflect the interplay of sulfide segregation/accumulation during magmatic differentiation, shedding light on these fundamental processes in Earth's interior.
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Triclocarban (TCC) is a widely used antibacterial ingredient possessing acute toxicity effects; however, its chronic toxicity and underlying molecular mechanisms remain uncertain. Herein, we demonstrated that chronic TCC exposure affects the growth and development of adult zebrafish through inducing an intestinal flora disorder in the gut. The imbalance of intestinal flora caused functional barriers within the intestinal-brain-gonadal axis. This resulted in a series of anomalous nerve and motor behaviors, and reproductive toxicity as reflected in pathological damage to parental gonads and F1-larval developmental malformations. Abnormal development of F1 larvae was attributed to apoptosis induced by the up-regulation of circSGOL1. This up-regulation affected the activity and localization of the hnRNP A1 protein, which then promoted overexpression of pro-apoptotic related genes that ultimately lead to apoptosis during early embryonic development. Overall, these novel findings systematically elucidated the TCC toxicity mechanism in parent-offspring dyads, and provide important theoretical guidance for early risk warning and control of chronic TCC toxicity.
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Poluentes Químicos da Água , Peixe-Zebra , Animais , Peixe-Zebra/metabolismo , Regulação para Cima , Poluentes Químicos da Água/toxicidade , Gônadas , Desenvolvimento EmbrionárioRESUMO
The prevalence of microplastics (MPs), especially aged particles, interacting with contaminants like triclosan (TCS), raises concerns about their toxicological effects on aquatic life. This study focused on the impact of aged polyamide (APA) MPs and TCS on zebrafish lipid metabolism. APA MPs, with rougher surfaces and lower hydrophobicity, exhibited reduced TCS adsorption than unaged polyamide (PA) MPs. Co-exposure to PA/APA MPs and TCS resulted in higher TCS accumulation in zebrafish larvae, notably more with PA than APA. Larvae exposed to PA + TCS exhibited greater oxidative stress, disrupted lipid metabolism, and altered insulin pathway genes than those exposed to TCS. However, these negative effects were lessened in the APA + TCS group. Through miRNA-seq and miR-217 microinjection, it was revealed that PA + TCS co-exposure upregulated miR-217, linked to lipid metabolic disorders in zebrafish. Moreover, molecular docking showed stable interactions formed between PA, TCS, and the insulin signaling protein Pik3r2. This study demonstrated that PA and TCS co-exposure significantly inhibited the insulin signaling in zebrafish, triggering lipid metabolism dysregulation mediated by miR-217 upregulation, while APA and TCS co-exposure alleviated these disruptions. This research underscored the ecological and toxicological risks of aged MPs and pollutants in aquatic environments, providing crucial insights into the wider implications of MPs pollution.
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Metabolismo dos Lipídeos , MicroRNAs , Microplásticos , Triclosan , Poluentes Químicos da Água , Peixe-Zebra , Animais , Larva/efeitos dos fármacos , Metabolismo dos Lipídeos/efeitos dos fármacos , Microplásticos/toxicidade , MicroRNAs/metabolismo , MicroRNAs/genética , Triclosan/toxicidade , Poluentes Químicos da Água/toxicidadeRESUMO
Triclosan (TCS), a prevalent contaminant in aquatic ecosystems, has been identified as a potential threat to both aquatic biota and human health. Despite its widespread presence, research into the immunotoxic effects of TCS on aquatic organisms is limited, and the underlying mechanisms driving these effects remain largely unexplored. Herein, we investigated the developmental and immune toxicities of environmentally relevant concentrations of TCS in zebrafish, characterized by morphological anomalies, histopathological impairments, and fluctuations in cytological differentiation and biomarkers following both acute (from 6 to 72/120 hpf) and chronic exposure periods (from 30 to 100 dpf). Specifically, acute exposure to TCS resulted in a significant increase in innate immune cells, contrasted by a marked decrease in T cells. Furthermore, we observed that TCS exposure elicited oxidative stress and a reduction in global m6A levels, alongside abnormal expressions within the m6A modification enzyme system in zebrafish larvae. Molecular docking studies suggested that mettl3 might be a target molecule for TCS interaction. Intriguingly, the knock-down of mettl3 mirrored the effects of TCS exposure, adversely impacting the growth and development of zebrafish, as well as the differentiation of innate immune cells. These results provide insights into the molecular basis of TCS-induced immunotoxicity through m6A-RNA epigenetic modification and aid in assessing its ecological risks, informing strategies for disease prevention linked to environmental contaminants.
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Triclosan , Poluentes Químicos da Água , Animais , Humanos , Triclosan/toxicidade , Triclosan/metabolismo , Peixe-Zebra/metabolismo , Regulação para Baixo , Metilação de RNA , Ecossistema , Simulação de Acoplamento Molecular , Poluentes Químicos da Água/toxicidade , Poluentes Químicos da Água/metabolismoRESUMO
BACKGROUND: The function of hematopoietic stem cells (HSC) is regulated by HSC internal signaling pathways and their microenvironment. Chemokines and chemokine ligands play important roles in the regulation of HSC function. Yet, their functions in HSC are not fully understood. METHODS: We established Cxcr3 and Cxcl10 knockout mouse models (Cxcr3-/- and Cxcl10-/-) to analyze the roles of Cxcr3 or Cxcl10 in regulating HSC function. The cell cycle distribution of LT-HSC was assessed via flow cytometry. Cxcr3-/- and Cxcl10-/- stem/progenitor cells showed reduced self-renewal capacity as measured in serial transplantation assays. To study the effects of Cxcr3 or Cxcl10 deficient bone marrow microenvironment, we transplanted CD45.1 donor cells into Cxcr3-/-or Cxcl10-/- recipient mice (CD45.2) and examined donor-contributed hematopoiesis. RESULTS: Deficiency of Cxcl10 and its receptor Cxcr3 led to decreased BM cellularity in mice, with a significantly increased proportion of LT-HSC. Cxcl10-/- stem/progenitor cells showed reduced self-renewal capacity in the secondary transplantation assay. Notably, Cxcl10-/- donor-derived cells preferentially differentiated into B lymphocytes, with skewed myeloid differentiation ability. Meanwhile, Cxcr3-deficient HSCs demonstrated a reconstitution disadvantage in secondary transplantation, but the lineage bias was not significant. Interestingly, the absence of Cxcl10 or Cxcr3 in bone marrow microenvironment did not affect HSC function. CONCLUSIONS: The Cxcl10 and Cxcr3 regulate the function of HSC, including self-renewal and differentiation, adding to the understanding of the roles of chemokines in the regulation of HSC function.
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Diferenciação Celular , Quimiocina CXCL10 , Células-Tronco Hematopoéticas , Receptores CXCR3 , Animais , Receptores CXCR3/metabolismo , Receptores CXCR3/genética , Quimiocina CXCL10/metabolismo , Quimiocina CXCL10/genética , Células-Tronco Hematopoéticas/metabolismo , Células-Tronco Hematopoéticas/citologia , Camundongos , Camundongos Knockout , Camundongos Endogâmicos C57BL , Autorrenovação Celular , Hematopoese , Transplante de Células-Tronco HematopoéticasRESUMO
Malignant tumors remain a high-risk disease with high mortality all over the world. Among all the cancer treatments, surgery is the primary approach in the clinical treatment of tumors. However, tumor invasion and metastasis pose challenges for complete tumor resection, accompanied by high recurrence rates and reduced quality of life. Hence, there is an urgent need to explore effective adjuvant therapies to prevent postoperative tumor recurrence and relieve the pain of the patients. Nowadays, the booming local drug delivery systems which can be applied as postoperative adjuvant therapies have aroused people's attention, along with the rapid development in the pharmaceutical and biological materials fields. Hydrogels are a kind of unique carrier with prominent biocompatibility among a variety of biomaterials. Due to their high similarity to human tissues, hydrogels which load drugs/growth factors can prevent rejection reactions and promote wound healing. In addition, hydrogels are able to cover the postoperative site and maintain sustained drug release for the prevention of tumor recurrence. In this review, we survey controlled drug delivery hydrogels such as implantable, injectable and sprayable formulations and summarize the properties required for hydrogels used as postoperative adjuvant therapies. The opportunities and challenges in the design and clinical application of these hydrogels are also elaborated.
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Hidrogéis , Recidiva Local de Neoplasia , Humanos , Recidiva Local de Neoplasia/tratamento farmacológico , Hidrogéis/uso terapêutico , Qualidade de Vida , Sistemas de Liberação de Medicamentos , Materiais Biocompatíveis/uso terapêuticoRESUMO
Azithromycin (AZM) is a widely used antibiotic in both human and veterinary medicine, and its use has significantly increased during the COVID-19 pandemic. However, potential adverse effects of AZM on aquatic organisms have not been well studied. In this study, we explored the neurotoxicity of AZM in zebrafish and delved into its underlying mechanisms. Our results showed that AZM exposure resulted in a spectrum of detrimental effects in zebrafish, encompassing abnormal behaviors, damaged neuronal development, aberrant lateral line nervous system development, vascular malformations and perturbed expression of genes related to neural development. Moreover, we observed a concentration-dependent exacerbation of these neurotoxic manifestations with increasing AZM concentrations. Notably, AZM induced excessive cell apoptosis and oxidative stress damage. In addition, alterations in the expression levels of the genes involved in the VEGF/Notch signaling pathway were evident in AZM-exposed zebrafish. Consequently, we hypothesize that AZM may induce neurotoxicity by influencing the VEGF/Notch signaling pathway. To validate this hypothesis, we introduced a VEGF signaling inhibitor, axitinib, and a Notch signaling agonist, valproic acid, alongside AZM exposure. Remarkably, the administration of these rescue compounds significantly mitigated the neurotoxic effects induced by AZM. This dual verification provides compelling evidence that AZM indeed induces neurotoxicity during the early developmental stages of zebrafish, primarily through its interference with the VEGF/Notch pathway. Innovatively, our study reveals the molecular mechanism of AZM-induced neurotoxicity from the perspective of the close connection between blood vessels and nervous system. These findings provide new insights into the potential mechanisms underlying the neurotoxic effect of antibiotics and highlight the need for further investigation into the ecotoxicological effects of antibiotics on aquatic organisms and the potential risks to human health.
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The main objective of this study was to determine the effect of filter pore size and fiber diameter on the performance of the baghouse filters for ultra-low emission. In this study, three kinds of conventional polyester filter (depth filtration media) and two kinds of polytetrafluoroethylene membrane-coated polyester filter (surface filtration media), having various filter pore sizes and fiber diameters, were tested to determine the performance of static and dynamic filtration. In order to determine the static filtration performance, the filtration resistance and the filtration efficiency of the clean filter media were measured by the arrestance method. The dynamic filtration performance experiments were conducted to determine the dynamic resistances, dust depositions, and dynamic filtration efficiencies of the dust-containing filter media under the condition of dust airflow filtration through a pulse-cleaning cycle. In the dynamic filtration performance experiments, the size of 50% test dust was less than 2.5 µm, and the mass mean aerodynamic diameter of the dust was 1.5 µm. The filtration velocity was 2 mâmin-1, and the dust concentration was 18.4 gâm-3. The static filtration performance experiments showed that the filter pore size greatly affected the filtration resistance and the filtration efficiency of the fabric structure of the surface filtration media. In the depth filtration media, the filtration efficiency and the filtration resistance of the fabric structure were improved when the filter pore size and the fiber diameter were smaller in magnitude. For all the five filter media, smaller the pore size of the filter media, greater was the filtration precision (for fine particles, such as PM2.5) of the fabric structure. In the dynamic filtration performance experiments, the filter pore size and the fiber diameter of the depth filtration media affected the dynamic filtration resistance and the dynamic filtration efficiency of the depth filtration media by affecting the deposition rate of dust in the depth filtration media; however, the filter pore size of the surface filtration media affected the blocking rate of dust in the membrane micropores, thus influencing the dynamic filtration resistance and the dynamic filtration efficiency of the surface filtration media.
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Poluição do Ar/prevenção & controle , Filtração/instrumentação , Poeira , Tamanho da Partícula , Poliésteres , PorosidadeRESUMO
Batch equilibrium experiments were used to reveal cyromazine adsorption on five kinds of soils, namely Ali-Perudic Ferrosols collected from Yingtan of Jiangxi, Udic Argosols collected from Nanjing and Gleyic-Stagnic Anthrosols collected from Changshu of Jiangsu, Ustic Cambosols collected from Fengqiu of Henan, and Udic Isohumosols collected from Hailun of Heilongjiang. Results show that the experimental data are best described by the Freundlich and Langmuir model, while fitted successfully by the linear model. Different adsorption behaviors of Cyromazine are observed in the five tested soils, with the lgK(f) values varying from 1.6505 (cambosols), 1.6715 (argosols) and 1.7153 (ferrosols) to 2.4579 (anthrosols) and 2.6557 (isohumosols). Moreover, the Kf values are in a positive correlation to the OM of the soil (r = 0.989) but significantly negative correlated to soil pH (r = -0.938). The free energy of sorption ranged from -20.8 to -23.0 kJ/mol, indicated that the adsorption could be largely attributed to the physical adsorption.