Antillean manatee (Trichechus manatus manatus Linnaeus, 1758) Tongue Morphology and Adaptive Herbivorous Implications.

Morphological study of the tongue is an interesting way of understanding evolutionary processes associated with feeding habits. Therefore, the aim of the present study was to describe the tongue morphology of the Antillean manatee and to understand possible morphological relationships with its way of capturing food. Macroscopic dissections and light and scanning electron microscopy analyses of seven manatee tongues were performed. The tongue in Antillean manatees is a muscular and robust organ, divided into apex, body, and root. It is firmly adhered to the floor of the oral cavity. Lingual papillae were distributed over the entire tongue surface. They were identified as filiform papillae concentrated in the apex. Fungiform papillae were present on the apex and lateral regions. Foliate papillae were located on the dorsolateral portion of the root. Lentiform papillae were located across the dorsal tongue surface. The mucosa was lined by a keratinized stratified squamous epithelium presenting compound tubuloacinar glands and taste buds in the foliate papillae. The tongue of the Antillean manatee is similar to other Sirenia species, both of which share a completely herbivorous diet.

TLR4 and TLR8 variability in Amazonian and West Indian manatee species from Brazil.

Amazonian (Trichechus inunguis) and West Indian (Trichechus manatus) manatees are aquatic mammals vulnerable to extinction found in the Amazon basin and the coastal western Atlantic. Toll-like receptors (TLR) play a key role in recognizing pathogen-associated molecular patterns using leucine-rich repeats (LRRs). We described the diversity of TLR4 and TLR8 genes in these two species of manatee. Amazonian manatee showed seven SNPs in TLR4 and the eight in TLR8, while West Indian manatee shared four and six of those SNPs, respectively. In our analysis, TLR4 showed one non-conservative amino acid replacement substitution in LRR7 and LRR8, on the other hand, TLR8 was less variable and showed only conserved amino acid substitutions. Selection analysis showed that only one TLR4 site was subjected to positive selection and none in TLR8. TLR4 in manatees did not show any evidence of convergent evolution compared to species of the cetacean lineage. Differences in TLR4 and TLR8 polymorphism may be related to distinct selection by pathogens, population reduction of West Indian manatees, or an expected consequence of population expansion in Amazonian manatees. Future studies combining pathogen association and TLR polymorphism may clarify possible roles of these genes and be used for conservation purposes of manatee species.

Brucella Infection Investigation in Cetaceans and Manatees in Northeast Brazil.

Among the bacterial infections that impair the health status of marine mammals, those caused by Brucella spp. are the most reported worldwide. Brucella infections in marine mammals can result in acute or chronic disease and are associated with variable clinical outcomes, depending on the organ involved during the infectious process, infection route, host immunity, and strain pathogenicity. Asymptomatic infections may also occur. The current study expands the investigation of Brucella infection in northeast Brazil by analyzing 19 dead, stranded cetaceans and 52 Antillean manatees Trichechus manatus manatus. The manatees included 8 dead, captive manatees and 44 live specimens, of which 10 were analyzed only after reintroduction into the wild as part of a rehabilitation program, 9 were analyzed both while in captivity or semi-captivity and after reintroduction, 20 were sampled only in captivity or semi-captivity, and 5 were free-living manatees. Serological tests were used to screen for antibodies against smooth Brucella spp. Whole blood, swabs, and tissue samples were screened for Brucella spp. DNA by PCR. Samples with positive PCR results were cultured for Brucella spp. isolation. All manatees yielded negative results in serological and molecular tests. Brucella spp. DNA was detected in the kidney of one adult Guiana dolphin Sotalia guianensis exhibiting necrosis in the liver. No growth of Brucella spp. was observed via microbiological culturing. This study is the first report of Brucella spp. DNA detection in cetaceans in the state of Pernambuco, and it highlights the importance of conducting systematic monitoring for the presence of Brucella infection in marine mammals along the Brazilian coast, especially in the northeast region, where several cases have been reported.

Novel insights on aquatic mammal MHC evolution: Evidence from manatee DQB diversity.

The low diversity in marine mammal major histocompatibility complex (MHC) appears to support the hypothesis of reduced pathogen selective pressure in aquatic systems compared to terrestrial environments. However, the lack of characterization of the aquatic and evolutionarily distant Sirenia precludes drawing more generalized conclusions. Therefore, we aimed to characterize the MHC DQB diversity of two manatee species and compare it with those reported for marine mammals. Our results identified 12 and 6 alleles in T. inunguis and T. manatus, respectively. Alleles show high rates of nonsynonymous substitutions, suggesting loci are evolving under positive selection. Among aquatic mammals, Pinnipeda DQB had smaller numbers of alleles, higher synonymous substitution rate, and a dN/dS ratio closer to 1, suggesting it may be evolving under more relaxed selection compared to fully aquatic mammals. This contradicts one of the predictions of the hypothesis that aquatic environments impose reduced pathogen pressure to mammalian immune system. These results suggest that the unique evolutionary trajectories of mammalian MHC may impose challenges in drawing ecoevolutionary conclusions from comparisons across distant vertebrate lineages.

Amazonian Manatees (Trichechus inunguis) inhabiting ans Equatorial Metropolis: historical records and mating activity near Belém, Northern Brazil

O peixe-boi das Índias Ocidentais Trichechus manatus Linnaeus, 1758 e o peixe-boi-amazônico T. inunguis (Natterer, 1883), ocorrem na Baía do Marajó e nas enseadas e canais próximos de Belém, Pará, Brasil. O Grupo de Estudos de Mamíferos Aquáticos da Amazônia (GEMAM) coordena desde 2005 uma rede colaborativa de informações sobre peixes-bois avistados e encalhados vivos ao longo do litoral do estado do Pará, incluindo os arredores e arquipélago de Belém. A presença do peixe-boi amazônico é confirmada por meio do resgate de diversos exemplares nas proximidades da grande Belém e registros visuais realizados durante monitoramento da área. As amostras desses peixes-bois estão alojadas nas coleções do MPEG e ICMBio, CMA. Em outubro de 2021, peixesbois- amazônicos foram avistados nadando muito próximo a uma praia no distrito de Mosqueiro, em Belém. Moradores e turistas fizeram imagens que evidenciam o comportamento de acasalamento relatado na literatura para Trichechus spp. As águas nessa área são normalmente muito túrbidas, e estes registros representam uma ótima oportunidade para entender esse comportamento. O recente evento de acasalamento sugere que os peixes-boi-amazônicos estão reocupando toda a região da grande Belém e seus arredores, bem como o litoral leste do estado do Pará. Os efeitos sinérgicos da pandemia de SARS-CoV-2, com a diminuição de pessoas nas praias, e o cenário atual de mudanças climáticas podem estar agindo combinadamente, o que seria uma esperança para esse sirênio ameaçado de extinção.

Debris ingestion by the Antillean Manatee (Trichechus manatus manatus).

The Antillean manatee inhabits coastal regions of North and Northeastern Brazil and currently is considered an endangered species in the country. Aiming to gather information for the development of public policies focusing on the conservation of manatees, the National Center for Research and Conservation of Aquatic Mammals of the Chico Mendes Institute for Biodiversity has been rescuing, rehabilitating and releasing these mammals since the 1980s. Over the last 36 years, 40 manatees were released by the CMA/ICMBio and four of them were rescued again due to debris ingestion. Two of these manatees died and the other two were taken back into captivity for a new rehabilitation process. The four mammals had confirmed diagnosis of plastic debris ingestion. These findings demonstrate that the environment where the manatees live after being released had a significant amount of garbage which may hinder the success of the species conservation in Brazil.