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1.
J Gen Virol ; 103(11)2022 11.
Artigo em Inglês | MEDLINE | ID: mdl-36399124

RESUMO

Currently, many viruses are classified based on their genome organization and nucleotide/amino acid sequence identities of their capsid and replication-associated proteins. Although biological traits such as vector specificities and host range are also considered, this later information is scarce for the majority of recently identified viruses, characterized only from genomic sequences. Accordingly, genomic sequences and derived information are being frequently used as the major, if not only, criteria for virus classification and this calls for a full review of the process. Herein, we critically addressed current issues concerning classification of viruses in the family Betaflexiviridae in the era of high-throughput sequencing and propose an updated set of demarcation criteria based on a process involving pairwise identity analyses and phylogenetics. The proposed framework has been designed to solve the majority of current conundrums in taxonomy and to facilitate future virus classification. Finally, the analyses performed herein, alongside the proposed approaches, could be used as a blueprint for virus classification at-large.


Assuntos
Flexiviridae , Vírus , Flexiviridae/genética , Genoma Viral , Vírus/genética , Filogenia , Sequenciamento de Nucleotídeos em Larga Escala
2.
Virol J ; 19(1): 93, 2022 05 26.
Artigo em Inglês | MEDLINE | ID: mdl-35619142

RESUMO

BACKGROUND: Phylogenetic studies indicate bats as original hosts of SARS-CoV-2. However, it remains unclear whether other animals, including pets, are crucial in the spread and maintenance of COVID-19 worldwide. METHODS: In this study, we analyzed the first fatal case of a SARS-CoV-2 and FeLV co-infection in an eight-year-old male cat. We carried out a clinical evaluation and several laboratory analyses. RESULTS: As main results, we observed an animal presenting severe acute respiratory syndrome and lesions in several organs, which led to the animal's death. RT-qPCR analysis showed a SARS-CoV-2 as the causative agent. The virus was detected in several organs, indicating a multisystemic infection. The virus was found in a high load in the trachea, suggesting that the animal may have contribute to the transmission of the virus. The whole-genome sequencing revealed an infection by SARS-CoV-2 Gamma VOC (P.1), and any mutations indicating host adaptation were observed. CONCLUSION: Our data show that FeLV-positive cats are susceptible to SARS-CoV-2 infection and raise questions about the potential of immunocompromised FeLV-positive cats to act as a reservoir for SARS-CoV-2 new variants.


Assuntos
COVID-19 , SARS-CoV-2 , Animais , Humanos , Vírus da Leucemia Felina , Masculino , Filogenia , SARS-CoV-2/genética
3.
Arch Virol ; 164(8): 2175-2178, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31102051

RESUMO

Sweet potato chlorotic stunt virus (SPCSV; genus Crinivirus, family Closteroviridae), is an economically important pathogen of sweet potato. In the present work, the nucleotide sequences of two RNA segments of SPCSV (isolate SPCSV-UNB-01) were determined by MiSeq Illumina sequencing of samples of sweet potato plants grafted onto Ipomoea setosa. A comparative analysis of the genome organization of SPCSV-UNB-01 and other SPCSV sequences showed that RNA1 was lacking p22, and p5.1 and that p5.2. was absent in RNA2, indicating a unique genomic pattern. SPCSV-UNB-01 contained longer p6 and p5 regions, with little similarity to orthologous sequences. Sequence comparison did not reveal any previously identified functional domains within these open reading frames (ORFs). No recombination or rearrangement events were detected. Phylogenetic analysis suggested the possibility of separate entries of SPCSV into South America based on the genetic distance between SPCSV-UNB-01 and the Peruvian isolate m2-47. Samples from northeastern Brazil (State of Pernambuco) were positive for SPCSV when tested using specific primers for the major coat protein (CP) gene. This is the first full-length genome sequence of SPCSV-UNB-01 from Brazil.


Assuntos
Crinivirus/genética , Crinivirus/isolamento & purificação , Genoma Viral/genética , Brasil , Crinivirus/classificação , Ipomoea batatas/virologia , Fases de Leitura Aberta/genética , Filogenia , Doenças das Plantas/virologia , RNA Viral/genética , Proteínas Virais/genética
4.
Virol J ; 15(1): 24, 2018 01 26.
Artigo em Inglês | MEDLINE | ID: mdl-29373979

RESUMO

The Tomato chlorotic spot virus (TCSV) was first reported in the 1980s, having its occurrence limited to Brazil and Argentina. Due to an apparent mild severity in the past, molecular studies concerning TCSV were neglected. However, TCSV has disseminated over the USA and Caribbean countries. In Dominican Republic TCSV has been recently reported on important cultivated crops such as pepper and beans. In this work, we provide the first complete genome of a TCSV isolate from symptomatic plants in Dominican Republic, which was obtained by high-throughput sequencing. In addition, three dsRNA viruses from different virus families were identified coinfecting these plants Bell pepper endornavirus (BPEV), Southern tomato virus (STV) and Pepper cryptic virus 2 (PCV-2). Phylogenetic analysis showed that the Dominican Republic TCSV isolate has a close relationship with other TCSV isolates and a reassortant isolate between TCSV and Groundnut ringspot virus (GRSV), all found in USA. BPEV, STV and PCV-2 isolates from Dominican Republic were close related to corresponding American isolates. The possible biological implications of these virus-mixed infections are discussed.


Assuntos
Coinfecção , Genoma Viral , Doenças das Plantas/virologia , Vírus de RNA/classificação , Vírus de RNA/genética , Tospovirus/classificação , Tospovirus/genética , Verduras/virologia , República Dominicana , Sequenciamento de Nucleotídeos em Larga Escala , Fenótipo , Filogenia , Vírus de RNA/isolamento & purificação , RNA de Cadeia Dupla , RNA Viral , Tospovirus/isolamento & purificação
5.
Arch Virol ; 162(1): 317-319, 2017 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-27730382

RESUMO

The complete genome sequence of a new virus infecting yam plants exhibiting mosaic symptom in Brazil was determined. The genome of this virus is composed of two molecules of positive-sense RNAs of 5979 and 3809 nucleotides in length, excluding the poly(A) tails. One large open reading frame (ORF) in each genomic segment (RNA1-ORF1 and RNA2-ORF2) was predicted. The highest amino acid sequence similarity in the Pro-Pol core region of RNA1 and the CP region of RNA2 was observed with chocolate lily virus A (a putative member of the family Secoviridae), with 54.6 and 27.7 % identity, respectively. This virus is thus likely to be a new member of the family Secoviridae, and we propose the tentative name "dioscorea mosaic-associated virus" (DMaV) for this virus.


Assuntos
Dioscorea/virologia , Genoma Viral , Vírus de Plantas/isolamento & purificação , Vírus de RNA/isolamento & purificação , RNA Viral/genética , Análise de Sequência de DNA , Brasil , Análise por Conglomerados , Fases de Leitura Aberta , Filogenia , Doenças das Plantas/virologia , Vírus de Plantas/classificação , Vírus de Plantas/genética , Vírus de RNA/classificação , Vírus de RNA/genética , Homologia de Sequência de Aminoácidos
6.
BMC Bioinformatics ; 17(Suppl 18): 489, 2016 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-28105914

RESUMO

BACKGROUND: Tospovirus is a plant-infecting genus within the family Bunyaviridae, which also includes four animal-infecting genera: Hantavirus, Nairovirus, Phlebovirus and Orthobunyavirus. Compared to these members, the structures of Tospovirus proteins still are poorly understood. Despite multiple studies have attempted to identify candidate N protein regions involved in RNA binding and protein multimerization for tospovirus using yeast two-hybrid systems (Y2HS) and site-directed mutagenesis, the tospovirus ribonucleocapsids (RNPs) remains largely uncharacterized at the molecular level and the lack of structural information prevents detailed insight into these interactions. RESULTS: Here we used the nucleoprotein structure of LACV (La Crosse virus-Orthobunyavirus) and molecular dynamics simulations to access the structure and dynamics of the nucleoprotein from tospovirus GRSV (Groundnut ringspot virus). The resulting model is a monomer composed by a flexible N-terminal and C-terminal arms and a globular domain with a positively charged groove in which RNA is deeply encompassed. This model allowed identifying the candidate amino acids residues involved in RNA interaction and N-N multimerization. Moreover, most residues predicted to be involved in these interactions are highly conserved among tospoviruses. CONCLUSIONS: Crucially, the interaction model proposed here for GRSV N is further corroborated by the all available mutational studies on TSWV (Tomato spotted wilt virus) N, so far. Our data will help designing further and more accurate mutational and functional studies of tospovirus N proteins. In addition, the proposed model may shed light on the mechanisms of RNP shaping and could allow the identification of essential amino acid residues as potential targets for tospovirus control strategies.


Assuntos
Nucleoproteínas/química , Tospovirus/química , Sequência de Aminoácidos , Sequência de Bases , Simulação de Dinâmica Molecular , Dados de Sequência Molecular , Nucleoproteínas/genética , Alinhamento de Sequência , Tospovirus/genética
7.
Mem Inst Oswaldo Cruz ; 110(6): 822-3, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-26517667

RESUMO

Bacillus thuringiensis is a ubiquitous Gram-positive and sporulating bacterium. Its crystals and secreted toxins are useful tools against larvae of diverse insect orders and, as a consequence, an alternative to recalcitrant chemical insecticides. We report here the draft genome sequence of B. thuringiensis147, a strain isolated from Brazil and with high insecticidal activity. The assembled genome contained 6,167,994 bp and was distributed in seven replicons (a chromosome and 6 plasmids). We identified 12 coding regions, located in two plasmids, which encode insecticidal proteins.


Assuntos
Bacillus thuringiensis/genética , DNA Bacteriano/análise , Inseticidas , Bacillus thuringiensis/classificação , Brasil , Plasmídeos/genética , Replicon/genética , Análise de Sequência de DNA
8.
BMC Genomics ; 15: 856, 2014 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-25280947

RESUMO

BACKGROUND: Cassava (Manihot esculenta) is the basic source for dietary energy of 500 million people in the world. In Brazil, Erinnyis ello ello (Lepidoptera: Sphingidae) is a major pest of cassava crops and a bottleneck for its production. In the 1980s, a naturally occurring baculovirus was isolated from E. ello larva and successfully applied as a bio-pesticide in the field. Here, we described the structure, the complete genome sequence, and the phylogenetic relationships of the first sphingid-infecting betabaculovirus. RESULTS: The baculovirus isolated from the cassava hornworm cadavers is a betabaculovirus designated Erinnyis ello granulovirus (ErelGV). The 102,759 bp long genome has a G + C content of 38.7%. We found 130 putative ORFs coding for polypeptides of at least 50 amino acid residues. Only eight genes were found to be unique. ErelGV is closely related to ChocGV and PiraGV isolates. We did not find typical homologous regions and cathepsin and chitinase homologous genes are lacked. The presence of he65 and p43 homologous genes suggests horizontal gene transfer from Alphabaculovirus. Moreover, we found a nucleotide metabolism-related gene and two genes that could be acquired probably from Densovirus. CONCLUSIONS: The ErelGV represents a new virus species from the genus Betabaculovirus and is the closest relative of ChocGV. It contains a dUTPase-like, a he65-like, p43-like genes, which are also found in several other alpha- and betabaculovirus genomes, and two Densovirus-related genes. Importantly, recombination events between insect viruses from unrelated families and genera might drive baculovirus genomic evolution.


Assuntos
Genoma Viral , Granulovirus/genética , Lepidópteros/virologia , Animais , Bases de Dados Genéticas , Granulovirus/classificação , Granulovirus/isolamento & purificação , Larva/virologia , Lepidópteros/crescimento & desenvolvimento , Manihot/parasitologia , Fases de Leitura Aberta/genética , Filogenia , Pirofosfatases/genética , Análise de Sequência de DNA , Proteínas Virais/classificação , Proteínas Virais/genética
9.
Virus Genes ; 49(3): 477-84, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25212430

RESUMO

Brazil is one of the largest silk producers in the world. The domesticated silkworm (Bombyx mori) was formally introduced into the country in the twentieth century, and the state of Paraná is the main national producer. During larval stages, B. mori can be afflicted by many different infectious diseases, which lead to substantial losses in silk production. In this work, we describe the structure and complete genome sequence of the first non-Asian isolate of Bombyx mori nucleopolyhedrovirus (BmNPV), the most important silkworm pathogen. The BmNPV-Brazilian isolate is a nucleopolyhedrovirus with singly enveloped nucleocapsids within polyhedral occlusion bodies. Its genome has 126,861 bp with a G + C content of 40.4 %. Phylogenetic analysis clustered the virus with the Japanese strain (BmNPV-T3). As expected, we have detected intra-population variability in the virus sample. Variation along homologous regions (HRs) and bro genes was observed; there were seven HRs, deletion of bro-e, and division of bro-a into two ORFs. The study of baculoviruses allows for a better understanding of virus evolution providing insight for biological control of insect pests or protection against the pernicious disease caused by these viruses.


Assuntos
DNA Viral/química , DNA Viral/genética , Genoma Viral , Nucleopoliedrovírus/genética , Animais , Composição de Bases , Bombyx/virologia , Brasil , Análise por Conglomerados , Dados de Sequência Molecular , Nucleopoliedrovírus/isolamento & purificação , Filogenia , Análise de Sequência de DNA
10.
Virus Genes ; 49(1): 169-73, 2014 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-24756556

RESUMO

Pepper ringspot virus (PepRSV) is a tobravirus reported only in Brazil. Here, the sequences of the complete RNA 2 segments and the 3' end of the RNA 1 genomic regions of two new isolates from tomato plants were analyzed. The main ORF encodes the CP gene as other tobraviruses and termed ORF 1 of RNA 2. The second ORF was found only in one of the new isolates, although this gene was absent in the type isolate, CAM (collected in the 1960's). Interestingly, this ORF 2 gene did not show any nucleotide and amino acid sequence similarities with known 2b genes of tobraviruses, an essential gene of tobraviruses for nematodes-transmission. The 5'UTR sequence of RNA 2 segment of CAM isolate was previously reported showing two impaired direct repeats; however, the direct-repeats were absent in these new isolates. An additional ORF was predicted upstream of the CP gene. This putative protein possessed a transmembrane domain similar to the ORFN1 of RNA 2 of Tobacco rattle virus SYM isolate, although there was no sequence similarity. This is the first report on the diversity of the RNA 2 sequences of PepRSV.


Assuntos
Vírus de Plantas/genética , Vírus de Plantas/isolamento & purificação , Vírus de RNA/genética , Vírus de RNA/isolamento & purificação , RNA Viral/genética , Solanum lycopersicum/virologia , Brasil , Dados de Sequência Molecular , Fases de Leitura Aberta , Doenças das Plantas/virologia , Análise de Sequência de DNA , Homologia de Sequência , Proteínas Virais/genética
11.
Trop Plant Pathol ; : 1-9, 2023 Apr 28.
Artigo em Inglês | MEDLINE | ID: mdl-37362078

RESUMO

Frequent monitoring of emerging viruses of agricultural crops is one of the most important missions for plant virologists. A fast and precise identification of potential harmful viruses may prevent the occurrence of serious epidemics. Nowadays, high-throughput sequencing (HTS) technologies became an accessible and powerful tool for this purpose. The major discussion of this strategy resides in the process of sample collection, which is usually laborious, costly and nonrepresentative. In this study, we assessed the use of sewage water samples for monitoring the widespread, numerous, and stable plant viruses using HTS analysis and RT-qPCR. Plant viruses belonged to 12 virus families were found, from which Virgaviridae, Solemoviridae, Tymoviridae, Alphaflexiviridae, Betaflexiviridae, Closteroviridae and Secoviridae were the most abundant ones with more than 20 species. Additionally, we detected one quarantine virus in Brazil and a new tobamovirus species. To assess the importance of the processed foods as virus release origins to sewage, we selected two viruses, the tobamovirus pepper mild mottle virus (PMMoV) and the carlavirus garlic common latent virus (GarCLV), to detect in processed food materials by RT-qPCR. PMMoV was detected in large amount in pepper-based processed foods and in sewage samples, while GarCLV was less frequent in dried and fresh garlic samples, and in the sewage samples. This suggested a high correlation of virus abundance in sewage and processed food sources. The potential use of sewage for a virus survey is discussed in this study. Supplementary Information: The online version contains supplementary material available at 10.1007/s40858-023-00575-8.

12.
Viruses ; 14(4)2022 03 23.
Artigo em Inglês | MEDLINE | ID: mdl-35458389

RESUMO

Tocantins is a state in the cross-section between the Central-West, North and Northeast regions of Brazilian territory; it is a gathering point for travelers and transportation from the whole country. In this study, 9493 genome sequences, including 241 local SARS-CoV-2 samples (collected from 21 December 2020, to 16 December 2021, and sequenced in the MinION platform) were analyzed with the following aims: (i) identify the relative prevalence of SARS-CoV-2 lineages in the state of Tocantins; (ii) analyze them phylogenetically against global SARS-CoV-2 sequences; and (iii) hypothesize the viral dispersal routes of the two most abundant lineages found in our study using phylogenetic and phylogeographic approaches. The performed analysis demonstrated that the majority of the strains sequenced during the period belong to the Gamma P.1.7 (32.4%) lineage, followed by Delta AY.99.2 (27.8%), with the first detection of VOC Omicron. As expected, there was mainly a dispersion of P.1.7 from the state of São Paulo to Tocantins, with evidence of secondary spreads from Tocantins to Goiás, Mato Grosso, Amapá, and Pará. Rio de Janeiro was found to be the source of AY.99.2 and from then, multiple cluster transmission was observed across Brazilian states, especially São Paulo, Paraiba, Federal District, and Tocantins. These data show the importance of trade routes as pathways for the transportation of the virus from Southeast to Northern Brazil.


Assuntos
COVID-19 , SARS-CoV-2 , Brasil/epidemiologia , COVID-19/epidemiologia , Genômica , Humanos , Filogenia , SARS-CoV-2/genética
13.
Virus Res ; 302: 198494, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-34174341

RESUMO

Anophelinae is a widely dispersed Culicidae subfamily that may carry a unique virome. Here we herein report the set of viruses found in 323 salivary glands of 16 anopheline species sampled at Upper Pantanal, Chapada dos Guimarães National Park and Coxipó river basin, South Central Mato Grosso, Brazil, pooled (n = 11) and subjected to high throughput sequencing. Metagenomics revealed the presence of nine viral sequences belonging to novel viruses from seven viral families: Purunga is a putative novel orbivirus sharing 74% and 65% aa identity, respectively, with the VP1 and VP3 segments of Changuinola serogroup, Jaracatiá flavivirus shares 60% amino-acid (aa) identity with Aedes flavivirus. Coxipó dielmovirus and Chapada dielmovirus shared 51% and 39% aa identity with Merida virus. Coloiado-orthomyxo like virus is 57.1-64.8% identical at aa level to Aedes albonnulatus orthomyxo-like virus. Mujica picorna-like virus shares 49% aa identity with Flen picorna-like virus and Chiquitos virus is 50% similar to Ista virus, both from Picornavirales order. Cerrado partiti-like-virus shares 75-86% aa identity with Atrato partiti-like virus 2. We also found the S and L segments of Anopheles triannulatus orthophasmavirus (92% identity) in Anopheles lutzi from Chapada dos Guimarães. The identification of these putative novel viruses underscore the wide dispersion of viruses in culicid hosts contributing to extensions on mosquito virome descriptions.


Assuntos
Filogenia , Animais , Brasil , Humanos
14.
Virus Res ; 293: 198257, 2021 02.
Artigo em Inglês | MEDLINE | ID: mdl-33309914

RESUMO

Forage crops occupy large areas of tropical pastures for cattle feeding in Brazil. The use of stylos (Stylosanthes spp.) in these pastures, which are leguminous shrubs, has increased in the country due to their outstanding nutritional value and for being an efficient and alternative source for nitrogen fixation in the soil. In recent years, virus-like mosaic symptoms on S.guianensis leaves have often been observed in the field, indicating possible virus-like pathogen infections. In an effort to identify the causal agent, virus semi-purification protocol was performed using symptomatic S. guianensis leaves collected at EMBRAPA Beef Cattle Research Center. Total RNA extracted from this semi-purified preparation was submitted to high-throughput sequencing, which revealed complete genome sequences of novel viruses of the family Potyviridae. These viruses, tentatively named stylo mosaic-associated virus 1 (StyMaV-1) and stylo mosaic-associated virus 2 (StyMaV-2), shared 73 % CP aa identity and 77 % polyprotein aa identity with each other and, after that, being closest related to blackberry virus Y, genus Brambyvirus (only 41 % CP aa identity). Based on ICTV genus demarcation criteria, StyMaV-1 and StyMaV-2 represent new species of a new genus within the family Potyviridae. StyMaV-1 and StyMaV-2 are also not efficiently transmitted to other plant species by mechanical inoculation.


Assuntos
Potyviridae , Animais , Brasil , Bovinos
15.
Infect Genet Evol ; 80: 104179, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-31917361

RESUMO

The World Health Organization (WHO), reports that rabies causes tens of thousands of deaths every year killing humans, non-human primates and other animals. Rabies continues to be a public health issue, despite the existence of effective vaccines. The dogs remain the primary reservoir and transmitter of rabies to humans globally. In the Americas, bats are regarded as the second most common source of rabies virus to humans. The vampire bat Desmodus rotundus has been identified as a natural reservoir of rabies virus (RABV) in this region. The complete genome of the RABV variant maintained by populations of vampire bats D. rotundus has rarely been reported. In this study, we sequenced and analyzed the genome of a RABV variant detected in D. rotundus. The sample, collected from an endemic area in São Paulo State, was phylogenetically compared with the genome of the standard sample for species Rabies virus as well as other samples belonging to terrestrial and bat-associated cycles of rabies transmission, available in GenBank. Distinct patterns linked to the genetic lineage were identified. These data can aid in the understanding of the molecular epidemiology of this virus and the epidemiological importance of this species in the transmission of the RABV.


Assuntos
Genoma Viral , Filogenia , Vírus da Raiva/classificação , Vírus da Raiva/genética , Raiva/virologia , Sequência de Aminoácidos , Animais , Biologia Computacional/métodos , Cães , Genômica/métodos , Humanos , Raiva/epidemiologia , Raiva/transmissão
16.
Arch Virol ; 154(10): 1685-7, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-19701602

RESUMO

Human parvovirus B19 is the only member of the genus Erythrovirus that causes human disease. Recent findings of several strains with considerable sequence divergence from B19 have suggested a new classification for parvovirus genotypes as 1 (B19), 2 (A-6 and LaLi) and 3 (V9). In their overall DNA sequence, the three genotypes differ by approximately 10%. Here, we report the isolation of a genotype-3-related strain named BR543 during a prospective study conducted in Sao Paulo, Brazil. Analysis of the nearly full-length genome sequence of BR543 indicates that this B19 variant sequence clusters with Gh2768, a strain from Ghana belonging to subtype 3b, and showed mostly synonymous substitutions.


Assuntos
Erythrovirus/genética , Sequência de Bases , Brasil , Variação Genética/genética , Genoma Viral/genética , Genótipo , Humanos , Dados de Sequência Molecular , Infecções por Parvoviridae/virologia , Parvovirus B19 Humano/genética , Filogenia
17.
Viruses ; 11(10)2019 10 17.
Artigo em Inglês | MEDLINE | ID: mdl-31627274

RESUMO

Viruses are ubiquitous and diverse microorganisms arising as a result of interactions within their vertebrate and invertebrate hosts. Here we report the presence of different viruses in the salivary glands of 1657 mosquitoes classified over 28 culicinae species from the North region of the Brazilian Pantanal wetland through metagenomics, viral isolation, and RT-PCR. In total, 12 viruses were found, eight putative novel viruses with relatively low similarity with pre-existing species of viruses within their families, named Pirizal iflavirus, Furrundu phlebovirus, Pixé phlebovirus, Guampa vesiculovirus, Chacororé flavivirus, Rasqueado orbivirus, Uru chuvirus, and Bororo circovirus. We also found the already described Lobeira dielmorhabdovirus, Sabethes flavivirus, Araticum partitivirus, and Murici totivirus. Therefore, these findings underscore the vast diversity of culicinae and novel viruses yet to be explored in Pantanal, the largest wetland on the planet.


Assuntos
Culicidae/virologia , Genoma Viral , Vírus de Insetos/classificação , Áreas Alagadas , Animais , Brasil , Vírus de DNA/classificação , Feminino , Sequenciamento de Nucleotídeos em Larga Escala , Metagenômica , Filogenia , Vírus de RNA/classificação , Glândulas Salivares/virologia
18.
Viruses ; 11(7)2019 07 03.
Artigo em Inglês | MEDLINE | ID: mdl-31277203

RESUMO

Baculoviruses are capable of infecting a wide diversity of insect pests. In the 1990s, the Dione juno nucleopolyhedrovirus (DijuNPV) was isolated from larvae of the major passionfruit defoliator pest Dione juno juno (Nymphalidae) and described at ultrastructural and pathological levels. In this study, the complete genome sequence of DijuNPV was determined and analyzed. The circular genome presents 122,075 bp with a G + C content of 50.9%. DijuNPV is the first alphabaculovirus completely sequenced that was isolated from a nymphalid host and may represent a divergent species. It appeared closely related to Orgyia pseudotsugata multiple nucleopolyhedrovirus (OpMNPV) and other Choristoneura-isolated group I alphabaculoviruses. We annotated 153 open reading frames (ORFs), including a set of 38 core genes, 26 ORFs identified as present in lepidopteran baculoviruses, 17 ORFs unique in baculovirus, and several auxiliary genes (e.g., bro, cathepsin, chitinase, iap-1, iap-2, and thymidylate kinase). The thymidylate kinase (tmk) gene was present fused to a dUTPase (dut) gene in other baculovirus genomes. DijuNPV likely lost the dut portion together with the iap-3 homolog. Overall, the genome sequencing of novel alphabaculoviruses enables a wide understanding of baculovirus evolution.


Assuntos
Borboletas/virologia , Nucleopoliedrovírus/classificação , Nucleopoliedrovírus/isolamento & purificação , Passiflora , Filogenia , Animais , Baculoviridae/classificação , Baculoviridae/genética , Composição de Bases , Sequência de Bases , Evolução Biológica , Mapeamento Cromossômico , Genoma Viral , Larva/virologia , Mariposas/virologia , Nucleopoliedrovírus/genética , Nucleopoliedrovírus/ultraestrutura , Fases de Leitura Aberta , Análise de Sequência de DNA , Sequenciamento Completo do Genoma
19.
Virus Res ; 253: 62-67, 2018 07 15.
Artigo em Inglês | MEDLINE | ID: mdl-29885325

RESUMO

The eucalyptus brown looper, Thyrinteina arnobia (Stoll, 1782) (Lepidoptera: Geometridae), is the main lepidopteran defoliator of eucalyptus plantations in Brazil. Outbreaks of this insect pest are common in Brazil and can affect the productivity of planted forests severely. T. arnobia caterpillars from a laboratory colony with viral infection symptoms were analyzed by electron microscopy that revealed polyhedral occlusion bodies (OBs) with several icosahedral virus particles embedded. Analysis of its genetic material showed ten segments of dsRNA, which confirmed this virus as a possible member of the genus Cypovirus. Phylogenetic analysis of the whole genome sequence revealed its close relationship with other isolates of Cypovirus 14 species and according to these results we proposed the name Thyrinteina arnobia cypovirus 14 (TharCPV-14) for this new virus isolate. Further research will be necessary in order to analyze the potential of this virus as a biopesticide.


Assuntos
Mariposas/virologia , Reoviridae/genética , Reoviridae/isolamento & purificação , Animais , Brasil , Eucalyptus/parasitologia , Genoma Viral , Genômica , Filogenia , Reoviridae/classificação
20.
Genome Announc ; 5(40)2017 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-28982994

RESUMO

The complete genome sequence of a novel bastrovirus was determined. The genome has 5,875 nucleotides and shares 56% nucleotide identity with a bastrovirus-like virus found in bat feces.

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