[Reduction of angialgia during peripheral administration of oxaliplatin mixed with dexamethasone].

Seven patients who had been receiving capecitabine+oxaliplatin±bevacizumab(CapeOX±BV)therapy at our hospital between February 2010 and March 2011, had complained of angialgia during oxaliplatin(L-OHP)administration. Therefore, 3. 3 mg of dexamethasone(DEX)was added to their infusion solution. The patients were then asked to rate their angialgia severity using a numerical rating scale(NRS), when L-OHP in a 5% dextrose solution was administered with or without DEX. By changing the L-OHP in 5% dextrose solution without DEX to the solution containing 3. 3 mg of DEX, the mean NRS was improved to 2. 4 from 7. 1. These findings indicate that L-OHP in 5% dextrose solution mixed with 3. 3 mg of DEX seems to be useful in reducing angialgia during peripheral administration of L-OHP.

Development and characterization of a monoclonal antibody with cross-reactivity towards uracil and thymine, and its potential use in screening patients treated with 5-fluorouracil for possible risks.

BACKGROUND: Dihydropyrimidine dehydrogenase (DPD) is the initial and rate-limiting enzyme in catabolism of pyrimidines including 5-fluorouracil. There have been efforts to isolate a monoclonal antibody that will bind selectively to pyrimidine and can be used to measure the concentration of pyrimidine in blood and/or in urine that may reflect the activity of dihydropyrimidine dehydrogenase. However, the monoclonal antibodies selective to pyrimidine have not been available. METHODS: Using 1-carboxymethyl-uracil as a hapten, in which steric conformation of uracil is thought to be well maintained, extensive screening was done to isolate a monoclonal antibody specific to uracil. RESULTS: We established the first monoclonal antibody that reacted with uracil and thymine but not with pseudouridine, dihydrouracil, dihydrothymine, cytosine, uridine, or N-carbamyl-beta-alanine at the concentration of 100 microg/ml. CONCLUSIONS: The monoclonal antibody can be used to develop a simple screening assay for patients with dihydropyrimidine dehydrogenase deficiency. This may increase the safety of 5-fluorouracil treatment.

Pigmented Plaques Associated with Papillomavirus Infection in Dogs: Is this Epidermodysplasia Verruciformis?

Abstract- Two Pugs and two Miniature Schnauzers with multiple pigmented epidermal nevi were investigated. The four dogs had pigmented cutaneous maculae and plaques. Histopathological evaluation showed papillated or digitated epidermal hyperplasia with hypermelanosis and giant keratohyalin granules in the stratum granulosum. Immunohistochemical staining revealed papillomavirus group-specific antigen in the skin specimens from all four dogs. Electron microscopic study of the specimens from two dogs revealed numerous round viral particles within the nuclei of the keratinocytes in the upper stratum granulosum. It was suspected that papillomavirus was the etiologic agent of the lesions, and that Pugs and Miniature Schnauzers might be predisposed to infection. These findings indicate this canine dermatosis resembles epidermodysplasia verruciformis (EV) of humans, a rare chronic disease caused by human papillomavirus. The potential for transformation of the lesions to squamous cell carcinoma is also suspected and discussed. Résumé- Deux Carlins et deux Schnauzers nains présentant de multiples naevi épidermiques pigmentés sont examinés. Les quatre chiens présentent des macules et des plaques pigmentées. Les lésions histopathologiques montrent une hyperplasie épidermique papillaire ou digitée avec une hypermélanose et la présence de grains de kératohyaline dans le stratum corneum. Les colorations immunohistochimiques révèlent des antigènes spécifiques du groupe des papillomas virus dans les biopsies des quatre chiens. L'étude ultrastructurale à partir des biopsies de deux chiens montrent de nombreuses particules virales rondes dans les noyaux des kératinocytes des couches supérieures du stratum granulosum. II a été suspecté que le papilloma virus était l'agent causal des lésions et que les Carlins et les Schnauzers nains pouvaient être prédisposés à cette infection. Ces éléments font que cette dermatose observée chez le chien ressemble à l'épidermodysplasie verruciforme de l'homme, une dermatose chronique rare causée par un papilloma virus humain. La potentialité de transformation des lésions en épithélioma spinocellulaire est aussi suspectée et discutée. [Nagata, M., Nanko, H., Moriyama, A., Washizu, T., Ishida, T. Pigmented plaques associated with papilloma virus infection in dogs: Is this epidermodysplasia verruciformis? (Plaques hyperpigmentées associées à une infection à papilloma virus chez le chien: est-ce épidermodysplasie verruciforme?). Resumen- Se investigó dos perros de raza Pug y dos de raza Schnauzer Miniatura con múltiples nevos epidérmicos pigmentados. Los cuatro perros presentaban máculas y placas cutáneas pigmentadas. El estudio histológico mostró hiperplasia epitelial con papilas y digitaciones, así como hipermelanosis y gránules de queratohialina gigantes en el estrato granuloso. Las tinciones immunohistoquimicas detectaron antígeno grupo-específico de papilomavirus en las muestras de los cuatro animales. Mediante estudios de microscopía electrónica en muestras de dos de los perros se observaron numerosas particulas virales redondas en el núcleo de los queratinocitos más superficiales del estrato granuloso. Se sospechó que Papilomavirus era el agente etiológico de estas lesiones y que los Pugs y los Schnauzer Miniatura podrian estar predispuestos a la infección. Estos hallazgos indican que esta dermatosis canina es parecida a la Epidermodisplasia Verruciforme (EV) de la especie humana, una enfermedad crónica rara causada por el Papilomavirus Humano. Se sospecha y discute la posible transformación de estas lesiones a carcinoma de células planas. [Nagata, M., Nanko, H., Moriyama, A., Washizu, T., Ishida, T. Pigmented plaques associated with papilloma virus infection in dogs: Is this epidermodysplasia verruciformis? (Placas pigmentadas asociadas con infección por papillomavirus en el perro: se trata de epidermodisplasia verruciforme?).