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1.
Proc Natl Acad Sci U S A ; 115(2): 361-366, 2018 01 09.
Artigo em Inglês | MEDLINE | ID: mdl-29279375

RESUMO

The wMel strain of Wolbachia can reduce the permissiveness of Aedes aegypti mosquitoes to disseminated arboviral infections. Here, we report that wMel-infected Ae. aegypti (Ho Chi Minh City background), when directly blood-fed on 141 viremic dengue patients, have lower dengue virus (DENV) transmission potential and have a longer extrinsic incubation period than their wild-type counterparts. The wMel-infected mosquitoes that are field-reared have even greater relative resistance to DENV infection when fed on patient-derived viremic blood meals. This is explained by an increased susceptibility of field-reared wild-type mosquitoes to infection than laboratory-reared counterparts. Collectively, these field- and clinically relevant findings support the continued careful field-testing of wMel introgression for the biocontrol of Ae. aegypti-born arboviruses.


Assuntos
Aedes/virologia , Vírus da Dengue/fisiologia , Dengue/virologia , Mosquitos Vetores/virologia , Wolbachia/fisiologia , Aedes/microbiologia , Animais , Dengue/sangue , Dengue/transmissão , Humanos , Modelos Logísticos , Mosquitos Vetores/microbiologia , Controle Biológico de Vetores/métodos , Fatores de Tempo , Viremia/sangue , Viremia/virologia
2.
Proc Natl Acad Sci U S A ; 110(22): 9072-7, 2013 May 28.
Artigo em Inglês | MEDLINE | ID: mdl-23674683

RESUMO

Dengue is the most prevalent arboviral disease of humans. The host and virus variables associated with dengue virus (DENV) transmission from symptomatic dengue cases (n = 208) to Aedes aegypti mosquitoes during 407 independent exposure events was defined. The 50% mosquito infectious dose for each of DENV-1-4 ranged from 6.29 to 7.52 log10 RNA copies/mL of plasma. Increasing day of illness, declining viremia, and rising antibody titers were independently associated with reduced risk of DENV transmission. High early DENV plasma viremia levels in patients were a marker of the duration of human infectiousness, and blood meals containing high concentrations of DENV were positively associated with the prevalence of infectious mosquitoes 14 d after blood feeding. Ambulatory dengue cases had lower viremia levels compared with hospitalized dengue cases but nonetheless at levels predicted to be infectious to mosquitoes. These data define serotype-specific viremia levels that vaccines or drugs must inhibit to prevent DENV transmission.


Assuntos
Aedes/virologia , Vírus da Dengue/genética , Dengue/epidemiologia , Dengue/transmissão , Dengue/virologia , Insetos Vetores/virologia , Animais , Sequência de Bases , Estudos de Coortes , Vírus da Dengue/classificação , Ensaio de Imunoadsorção Enzimática , Interações Hospedeiro-Patógeno , Humanos , Funções Verossimilhança , Modelos Genéticos , Dados de Sequência Molecular , Filogenia , Prevalência , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Análise de Sequência de DNA , Sorotipagem , Vietnã/epidemiologia , Viremia/epidemiologia
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