First record of Diamesus osculans (Vigors, 1825) (Coleoptera: Silphidae) colonization on a human corpse.

A partially skeletonized human corpse was found in bushes in Selangor, Malaysia in June 2020. Entomological evidence was collected during the autopsy and sent to the Department of Medical Microbiology and Parasitology, Faculty of Medicine, Universiti Teknologi MARA (UiTM) for minimum postmortem interval (PMImin) analysis. Standard protocols were applied when processing preserved and live insect specimens of both larval and pupal stages. Entomological evidence revealed that the corpse was colonized by Chrysomya nigripes Aubertin, 1932 (Diptera: Calliphoridae) and Diamesus osculans (Vigors, 1825) (Coleoptera: Silphidae). Chrysomya nigripes was chosen as the PMImin indicator as this fly species is an earlier colonizer compared to D. osculans beetle larvae which their presence is the indicative of late stage of decomposition. For the present case, the pupae of C. nigripes were the oldest insect evidence collected and based on the available developmental data, the estimated minimum PMI was established between 9 and 12 days. It is noteworthy to highlight that this is the first record of D. osculans colonization on a human corpse.

Black soldier fly (Diptera: Stratiomyidae) reduction of different sludges, subsequent safety, and research gaps.

One of the many waste components that end up in landfills is sludge, an organic waste that Black Soldier Flies (BSF) may be capable of reducing or removing along with potential pathogens from the environment. Throughout this review, knowledge gaps were evaluated as well as indications that BSF can positively impact the environment and economy when it comes to reducing sludge. This review first aimed to establish consistency across assessed papers (n = 41). Second, previous research on the efficiency and capability of sludge to be reduced by BSF both in isolation and with other substrates (co-digestion) was assessed. Subsequently, this review also evaluated the resulting safety of both the BSF and remaining sludge after consumption. Through this review, reduction difficulties and gaps in research and industry were evaluated. Unfortunately, complications come from the lack of policy for industry status as well as the need for further research. Therefore, further research is required on the consumption of different sludges. In particular, sludge should be tested with co-substrates to decrease waste removal problems. Sludge lacks crucial nutrients and contains extracellular polymeric substances preventing BSFL consumption. This review could potentially be helpful in future research to formulate a methodology for better BSFL consumption and production of insect biomass by sludge pre-treatment. This could lead to the implementation of the new policy in sludge management.