RESUMO
Glioma is one of the most common and aggressive malignant primary brain tumors with high recurrence rate and mortality rate and heavily depends on the angiogenesis. LncRNA H19 has many diverse biological functions, including the regulation of cell proliferation, differentiation and metabolism. Here, we aimed to investigate the molecular mechanism of lncRNA H19 affecting angiogenesis in glioma, which could help to uncover potential target for glioma therapy. RT-qPCR analysis was performed to detect the expression of lncRNA H19 and miR-138 in HEB, U87, A172 and U373 cell lines. MTT assay was used to evaluate the cell viability. To evaluate the migration and invasion after lncRNA H19 knockdown, Transwell and wound healing assay were employed. After lncRNA H19 knockdown, protein expression of HIF 1α and VEGF was detected by western blot and tube formation was assessed. For the prediction and validation of the interaction between lncRNA H19 and miR-138, bioinformatics and luciferase assay were performed. We investigated the regulatory roles and downstream molecular mechanisms of lncRNA H19 in glioma by knockdown H19, which inhibited the proliferation, migration and angiogenesis of glioma cells. Moreover, miR-138 acted as a target of H19 as detected by luciferase reporter assays. Meanwhile, HIF-1α was also a target of miR-138 and miR-138 could also regulate the proliferation, migration and angiogenesis of glioma cells by targeting HIF-1α and affecting the expression of VEGF in turn. Our findings identified an upregulated lncRNA H19 in glioma cells, which could promote proliferation, migration, invasion and angiogenesis via miR-138/HIF-1α axis as a ceRNA. This study provided a new opportunity to advance our understanding in the potential mechanism of lncRNA in glioma angiogenesis.
Assuntos
Glioma/patologia , Subunidade alfa do Fator 1 Induzível por Hipóxia/genética , MicroRNAs/genética , Neovascularização Patológica/genética , RNA Longo não Codificante/genética , Fator A de Crescimento do Endotélio Vascular/genética , Linhagem Celular Tumoral , Glioma/genética , HumanosRESUMO
Patients with severe alveolar ridge defects cannot be directly implanted and repaired, which seriously affects their quality of life. Onlay bone grafting is the main solution for severe alveolar ridge bone defect reconstruction, among which autogenous block bone grafting is the most widely used and is also the focus of clinical research on bone tissue reconstruction. This article expounds the characteristics and basic principles of autogenous bone block grafting, and comprehensively analyzes the selection of autogenous bone donor site, the principles of surgical operation, and the progress of bone graft techniques. In order to help surgeons make correct clinical decisions, increase the predictability of surgical effects, and improve the level of clinical diagnosis and treatment.
Assuntos
Processo Alveolar , Aumento do Rebordo Alveolar , Transplante Ósseo , Humanos , Transplante Ósseo/métodos , Aumento do Rebordo Alveolar/métodos , Processo Alveolar/anormalidades , Processo Alveolar/cirurgia , Transplante Autólogo , Procedimentos de Cirurgia Plástica/métodos , Perda do Osso Alveolar/cirurgia , Sítio Doador de TransplanteRESUMO
Five trace metals (Cd, Cu, Fe, Mn, and Zn) in the hair of healthy subjects(n = 10) and patients with benign prostatic hypertrophy (n = 17) and prostatic cancer (n = 18) were determined using atomic absorption spectrophotometry. The results were that zinc level in patients with prostatic carcinoma [(79.1 +/- 37.3) microgram.g-1] was significantly lower than that in patients with benign prostatic hypertrophy [(129 +/- 26.7) microgram.g-1] and normal controls [(152 +/- 31.5) microgram.g-1] (All P < 0.01); Cd, Cu, Fe, and Mn levels in the hair showed no statistic difference among the three groups (P > 0.05). It is suggested that the low zinc level in the hair might provide an important clue for diagnosing prostatic carcinoma at the early stage.