A novel model for predicting prognosis in patients with idiopathic pulmonary fibrosis based on endoplasmic reticulum stress-related genes.

Idiopathic pulmonary fibrosis (IPF) is a progressive fibrotic disease of unknown pathogenic origin. Endoplasmic reticulum (ER) stress refers to the process by which cells take measures to ER function when the morphology and function of the reticulum are changed. Recent studies have demonstrated that the ER was involved in the evolution and progression of IPF. In this study, we obtained transcriptome data and relevant clinical information from the Gene Expression Omnibus database and conducted bioinformatics analysis. Among the 544 ER stress-related genes (ERSRGs), 78 were identified as differentially expressed genes (DEGs). These DEGs were primarily enriched in response to ER stress, protein binding, and protein processing. Two genes (HTRA2 and KTN1) were included for constructing an accurate molecular signature. The overall survival of patients was remarkably worse in the high-risk group than in the low-risk group. We further analyzed the difference in immune cells between high-risk and low-risk groups. M0 and M2 macrophages were significantly increased in the high-risk group. Our results suggested that ERSRGs might play a critical role in the development of IPF by regulating the immune microenvironment in the lungs, which provide new insights on predicting the prognosis of patients with IPF.

Impaired autophagy-accelerated senescence of alveolar type II epithelial cells drives pulmonary fibrosis induced by single-walled carbon nanotubes.

BACKGROUND: The rapid increase in production and application of carbon nanotubes (CNTs) has led to wide public concerns in their potential risks to human health. Single-walled CNTs (SWCNTs), as an extensively applied type of CNTs, have shown strong capacity to induce pulmonary fibrosis in animal models, however, the intrinsic mechanisms remain uncertain. RESULTS: In vivo experiments, we showed that accelerated senescence of alveolar type II epithelial cells (AECIIs) was associated with pulmonary fibrosis in SWCNTs-exposed mice, as well as SWCNTs-induced fibrotic lungs exhibited impaired autophagic flux in AECIIs in a time dependent manner. In vitro, SWCNTs exposure resulted in profound dysfunctions of MLE-12 cells, characterized by impaired autophagic flux and accelerated cellular senescence. Furthermore, the conditioned medium from SWCNTs-exposed MLE-12 cells promoted fibroblast-myofibroblast transdifferentiation (FMT). Additionally, restoration of autophagy flux with rapamycin significantly alleviated SWCNTs-triggered senescence and subsequent FMT whereas inhibiting autophagy using 3-MA aggravated SWCNTs-triggered senescence in MLE-12 cells and FMT. CONCLUSION: SWCNTs trigger senescence of AECIIs by impairing autophagic flux mediated pulmonary fibrosis. The findings raise the possibility of senescence-related cytokines as potential biomarkers for the hazard of CNTs exposure and regulating autophagy as an appealing target to halt CNTs-induced development of pulmonary fibrosis.

Interaction of biomass fuels use and metals exposure on hypertension: A prospective cohort study in rural areas along the Yangtze River, China.

Humans maybe simultaneously exposed to multiple-metals and indoor air pollution in daily life. However, limited prospective studies have assessed the interaction between multiple metals exposure and biomass fuels use on hypertension in China. The prospective cohort study in rural areas along the Yangtze River included 2625 adults in 2014-2015, and they were followed up till 2019. Among 1248 rural residents who were without hypertension at baseline, 377 hypertension events (30.21%) were observed after 4.5 years of average follow-up time. First-morning urine samples of residents were collected at baseline, the association between urinary metals level and hypertension were assessed using quantile g-computation. Additionally, we also examined the effect of biomass fuels use, fuels switching, and cookstove ventilation on the association of metals exposure with hypertension. Quantile g-computation analyses showed a positive joint effect of 17 metals on hypertension, with the odds ratio (OR) of 1.68 (95% CI: 0.89, 3.14) when increasing all seventeen metals by one quartile, and cadmium, lithium, copper contributed the largest positive weights. Biomass fuels use can interact with cadmium exposure on hypertension with OR for interaction of 1.28 (95%CI: 1.00, 1.73), and increase the association between copper, manganese and zinc exposure and systolic blood pressure, lithium exposure and diastolic blood pressure at the follow-up visit. Moreover, switching from biomass fuels to clean fuels during follow up, cookstove ventilation can alleviate the risk of higher blood pressure from metals exposure. In rural areas along the Yangtze River, China, biomass fuels use for cooking can interact with multiple-metals exposure on hypertension. Residents who switched from biomass fuels to clean fuels and who used ventilation had a lower risk of hypertension. Further cohort studies are needed to clarify the mechanism of combined effects of metals exposure and biomass fuels use on the human health.

Patient-related factors that influence coronary artery density in CCTA: a retrospective clinical study.

OBJECTIVES: The aim of this study was to investigate the relationship between various patient-related factors (physical and cardiac hemodynamic parameters) and the coronary artery density on coronary CT angiography (CCTA). METHODS: A total of 64 patients (female: male ratio, 24:40; age, 58.2 years±9.3, age range, 31-81 years; mean body weight, 65.3 kg±11.6, range 40-88 kg) were effectively enrolled in this approved retrospective study. Patient-related physical factors including height, body weight (BW), body mass index (BMI), systolic blood pressure (BPsys), diastolic blood pressure (BPdis) and blood pulse pressure (Bp) were recorded, measured and calculated prior to the administration of contrast media during the CCTA. Patient-related cardiac hemodynamic parameters, including heart rate (HR), myocardial mass (MM), cardiac output (CO), ejection fraction (EF), end-diastolic dimension (EDV), end-systolic volume (ESV) and stroke volume (SV), were analysed and recorded on the multimodality workplace (MMWP). The mean attenuation values of the left main artery (LMA) were measured and calculated. The correlation of the mean attenuation in the coronary arteries with the physical and hemodynamic parameters was evaluated. The correlations between the physical factors and hemodynamic parameters were also calculated. RESULTS: A significant negative linear correlation was found between the attenuation of the left main artery (LMA) and BW (P=.001), BMI (P=.006), CO (P=.008), EDV (P=.001) and MM (P<.001). Significant linear correlations were obtained between CO and HR (P<.001), EDV and BW (P=.001) and MM and BW (P<.001). CONCLUSION: Coronary artery attenuation depends on the patient's specific physical and cardiac function status.

Nozzle Wear in Abrasive Water Jet Based on Numerical Simulation.

Particle diameters and jet pressure in abrasive water jet (AWJ) are significant jet properties which deserve a better understanding for improving AWJ machining performance. Some influence factors have been verified regarding nozzle wear in abrasive water jet polishing application. A three-dimensional model of a nozzle is established to analyze the influence of internal multi-phase flow field distribution, which is based on Euler-Lagrange methodology. With the increase of jet pressure, the erosion rate decreases; with the increase of the diameter and mass flow rate of the erosion particles, the erosion speed increases as well. When the diameter of the outlet is worn to 1.6 mm, the pressure on the work piece caused by the abrasive water jet increases by more than double compared to the non-worn nozzle; when the diameter of the nozzle outlet is worn to 1.6 mm, the shear force is 2.5 times higher than the shear force when the diameter is 1.0, which means that the jet force is divergent when the diameter is 1.6 mm, and the damage of the work piece is very serious. The obtained results could improve polishing efficiency on the work piece, extend nozzle lifetimes, and guide the future design of AWJ nozzles.

Environmental and occupational risk factors for COPD and its prevalence among miners worldwide: a Mendelian randomization and meta-analysis study.

Chronic obstructive pulmonary disease (COPD) is the third leading cause of death after cardiovascular disease and stroke, and its incidence is associated with genetic, environmental, and occupational factors. Miner is high-risk population for COPD, but the global prevalence of COPD in this group is inaccurate. In this study, the environmental and occupational risk factors for COPD were explored comprehensively with a two-sample Mendelian randomization study by combining genome-wide association data from two large global sample sizes of publicly available databases, UK Biobank (n = 503,317) and FinnGen (n = 193,638), as well as the prevalence of COPD among miners was investigated with meta-analysis followed a random-effects model including seven studies (16,033 miners in total). This study found that asthma, smoking, shift work, and workplace dust exposure may increase an individual's risk of COPD. The pooled prevalence of COPD among miners globally was 12% (95% CI: 8%, 18%), with higher prevalence of COPD among ex-smokers and dust-exposed individuals, and was significantly influenced by the method of diagnosis. Our findings suggest that there is currently a lack of practical criteria for diagnosing COPD in the physical examination and screening of miners. The actual prevalence of COPD may be underestimated due to the healthy worker effect and the phenomenon of job switching, and appropriate policies should be favored in the future to reduce the risk of COPD in miner.

[Evaluating radiation dose of 128-slice dual-source computed tomographic coronary angiography with different electrocardiogram pulsing models].

OBJECTIVE: To determine the effects of heart rate frequency (HRF) and heart rate variability (HRV) on radiation exposure and image quality in a large cohort of patients undergoing DSCT (dual-source computed tomograph) coronary angiography with three different electrocardiogram (ECG) pulsing models, to prospectively investigate CT image quality parameters by using different protocols and to calculate the radiation dose estimates for noninvasive coronary angiography performed by DSCT. METHODS: Over a 1-month period, 253 consecutive patients were recruited and categorized into 3 groups: Group A:low HRF (≤ 75 beats/min) with normal-minor heart rate variability (HRV) (mean interbeat difference (IBD), 0 - 3), coronary CT angiography proceeding with prospective ECG-triggered flash spiral scan; Group B: intermediate-high HRF (> 75 beats/min) with normal-minor HRV (IBD, 0 - 4), with prospective ECG-triggered sequence scan; Group C: intermediate-high HRF (> 75 beats/min) with moderate-severe HRV (IBD ≥ 5) with retrospective ECG-gated spiral scan. RESULTS: CT coronary angiography yielded excellent image quality in 87.7% of patients (222/253). No significant differences were found among three groups with different HRF and HRV in image quality and diagnostic performance. Radiation exposure was significantly higher in patients with low versus high HRF and in patients with severe versus normal HRV. Significant difference (P < 0.001) was found among the effective doses of group A (mean ± standard deviation, 0.602 ± 0.363 mSv), group B (1.253 ± 0.804 mSv) and group C (9.039 ± 5.657 mSv). CONCLUSION: The higher temporal resolution of dual-source spiral CT coronary angiography performed with adaptive ECG pulsing results in preserved diagnostic image quality and performance independent of HRF or HRV at the cost of limited dose reduction in arrhythmic patients.

Correlation of CT perfusion images with VEGF expression in solitary brain metastases.

OBJECTIVES: To obtain permeability surface (PS) values using multi-slice helical CT perfusion imaging and to evaluate the spatial distribution correlation between PS values and vascular endothelial growth factor (VEGF) expression in solitary brain metastases. METHODS: Imaging was performed on 21 patients, PS values being calculated from the central, border and peripheral parts of tumours. VEGF expression was determined by immunohistochemical staining. RESULTS: Rim enhancement was found in 16 cases, the border of the tumour featuring PS elevation with high VEGF expression in 13 cases. In the 5 cases with nodular enhancement, the border and the central part had high permeability and VEGF expression was high in all cases, the correlation being significant (P<0.01) . CONCLUSION: VEGF expression in brain metastases positively correlates with PS values from CT perfusion imaging, so that the latter can be used in the surveillance of angiogenic activity in brain metastases.