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1.
Adv Skin Wound Care ; 33(4): 217-220, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32195725

RESUMO

OBJECTIVE: To compare the efficacy of three topical agents commonly used in cutaneous wound healing. METHODS: Wound healing was studied in 29 participants, and each participant served as his or her own control. In each participant, three similarly sized and located seborrheic keratoses were removed by curettage. Resultant wounds were treated with either trolamine emulsion, manuka honey gel, or polymyxin-bacitracin ointment until the wounds were fully healed. RESULTS: Wounds treated with trolamine emulsion healed significantly faster than wounds treated with either manuka honey or polymyxin-bacitracin (15 vs 19 days; P < .001). CONCLUSIONS: Trolamine emulsion may be preferred in clinical practice to accelerate the healing time of clean, shallow wounds.


Assuntos
Bacitracina/administração & dosagem , Etanolaminas/administração & dosagem , Mel , Polimixina B/administração & dosagem , Pele/efeitos dos fármacos , Cicatrização/efeitos dos fármacos , Administração Cutânea , Emulsões/administração & dosagem , Géis/administração & dosagem , Humanos , Pomadas/administração & dosagem , Pele/lesões , Resultado do Tratamento
2.
Exp Dermatol ; 22(12): 781-4, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24131368

RESUMO

Grover's disease (GD) is a transient or persistent, monomorphous, papulovesicular, asymptomatic or pruritic eruption classified as non-familial acantholytic disorder. Contribution of autoimmune mechanisms to GD pathogenesis remains controversial. The purpose of this study was to investigate antibody-mediated autoimmunity in 11 patients with GD, 4 of which were positive for IgA and/or IgG antikeratinocyte antibodies by indirect immunofluorescence. We used the most sensitive proteomic technique for an unbiased analysis of IgA- and IgG-autoantibody reactivities. Multiplex analysis of autoantibody responses revealed autoreactivity of all 11 GD patients with cellular proteins involved in the signal transduction events regulating cell development, activation, growth, death, adhesion and motility. Semiquantitative fluorescence analysis of cultured keratinocytes pretreated with sera from each patient demonstrated decreased intensity of staining for desmoglein 1 and/or 3 and PCNA, whereas 4 of 10 GD sera induced BAD expression, indicating that binding of autoantibodies to keratinocytes alters expression/function of their adhesion molecules and activates apoptosis. We also tested the ability of GD sera to induce visible alterations of keratinocyte shape and motility in vitro but found no specific changes. Thus, our results demonstrated that humoral autoimmunity in GD can be mediated by both IgA and IgG autoantibodies. At this point, however, it is impossible to conclude whether these autoantibodies cause or are caused by the disease. Antidesmoglein antibodies may be triggered by exposure to immune system of sequestered antigens due to disintegration of desmosomes during primary acantholysis. Clarifying aetiology of GD will help improve treatment, which currently is symptomatic and of marginal effectiveness.


Assuntos
Acantólise/imunologia , Doenças Autoimunes/imunologia , Ictiose/imunologia , Dermatopatias/imunologia , Acantólise/diagnóstico , Idoso , Idoso de 80 Anos ou mais , Apoptose , Autoanticorpos/sangue , Autoimunidade/imunologia , Moléculas de Adesão Celular/imunologia , Desmossomos/metabolismo , Humanos , Ictiose/diagnóstico , Imunoglobulina A/sangue , Imunoglobulina G/sangue , Queratinócitos/citologia , Queratinócitos/imunologia , Pessoa de Meia-Idade
5.
Arch Dermatol Res ; 307(3): 275-80, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-25384437

RESUMO

Inflammation in atopic dermatitis is mediated in part by the chemokine CCL1 and its receptor, CCR8. Recombinant Molluscum contagiosum viral protein (rMC148p), a cc-chemokine homolog, inhibits CCL1-induced chemotaxis of cells expressing CCR8. rMC148p was prepared using the baculovirus/Sf9 insect cell expression system. The recombinant MC148 fusion protein (rMC148fp), rMC148-TAT-6xHis, was similarly prepared by adding base sequences onto the PCR primers to fuse TAT and 6xHis to rMC148p at the carboxyl terminus. rMC148fp retains the capacity of rMC148p to inhibit CCL1-induced chemotaxis. Furthermore, unlike rMC148p, topically applied rMC148fp penetrates stratum corneum of human neonatal foreskins and concentrates along the basal and lower spinous cell layers of the epidermis. rMC148fp may be a safe and effective agent in the treatment of atopic dermatitis and other CCR8-mediated disorders.


Assuntos
Quimiocina CCL1/metabolismo , Quimiocinas CC/administração & dosagem , Dermatite Atópica/terapia , Epiderme/efeitos dos fármacos , Imunoterapia , Receptores CCR8/metabolismo , Proteínas Recombinantes de Fusão/administração & dosagem , Administração Tópica , Animais , Baculoviridae/genética , Quimiotaxia/efeitos dos fármacos , Protocolos Clínicos , Clonagem Molecular , Dermatite Atópica/imunologia , Epiderme/imunologia , Prepúcio do Pênis/citologia , Humanos , Recém-Nascido , Masculino , Molusco Contagioso/genética , Molusco Contagioso/imunologia , Proteínas Recombinantes de Fusão/genética , Células Sf9 , Spodoptera
6.
Cutis ; 93(1): 40-2, 2014 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-24505583

RESUMO

A 14-year-old patient with severe widespread atopic dermatitis (AD) and concurrent molluscum contagiosum virus (MCV) presented with MCV papules that were surrounded by dermatitis-free zones. It appeared that components inhibited the expression of AD, with inhibitory effects that persisted throughout 6 years of follow-up, even in the presence of a Staphylococcus aureus infection, which was proven by skin culture.


Assuntos
Dermatite Atópica/virologia , Molusco Contagioso/virologia , Vírus do Molusco Contagioso/isolamento & purificação , Adolescente , Dermatite Atópica/patologia , Seguimentos , Humanos , Índice de Gravidade de Doença , Infecções Cutâneas Estafilocócicas/diagnóstico , Staphylococcus aureus/isolamento & purificação
7.
Int J Dermatol ; 45(4): 352-6, 2006 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-16650156

RESUMO

BACKGROUND: Atopic dermatitis (AD) is characterized both by inflammation and by abnormally low ceramide levels in the stratum corneum. Tacrolimus, effective in the treatment of AD, inhibits transcription and release of inflammatory cytokines operative in AD, but it is unknown if tacrolimus normalizes ceramide levels. METHODS: Stratum corneum ceramides of seven subjects with lesional AD were measured before and after treatment with tacrolimus ointment. RESULTS: Treatment with tacrolimus ointment did not increase the ceramide fractions toward or to normal. CONCLUSION: These findings suggest that tacrolimus ointment is effective in the treatment of atopic dermatitis due to its anti-inflammatory actions rather than to any restorative effect on stratum corneum ceramides.


Assuntos
Ceramidas/metabolismo , Dermatite Atópica/metabolismo , Imunossupressores/uso terapêutico , Pele/metabolismo , Tacrolimo/uso terapêutico , Adolescente , Adulto , Dermatite Atópica/tratamento farmacológico , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Pomadas , Projetos Piloto , Valores de Referência
8.
Int J Dermatol ; 45(4): 357-60, 2006 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-16650157

RESUMO

BACKGROUND: The study of skin barrier constituents may require collection of much stratum corneum. Existing methods are inadequate and/or difficult. METHODS: A simple and safe method to harvest stratum corneum from glabrous human skin derives from the bathing practices of people in Azerbaijan. The method requires water immersion of the subject for 30 min immediately followed by vigorous scrubbing of skin with a moist rough crepe mitten. RESULTS: This scrubbing method causes the separation of large amounts of stratum corneum which is easily harvested. CONCLUSION: The method facilitates study of stratum corneum components, including intercellular lipids.


Assuntos
Banhos , Imersão , Pele/patologia , Adulto , Idoso , Azerbaijão , Banhos/instrumentação , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
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