Occurrence and distribution of micro/nanoplastics in soils and their phytotoxic effects: A review.

Some recent studies have reviewed the occurrence and phytotoxicity of micro/nanoplastics, but their distribution in the soil environment, mechanisms of uptake by roots and the mode of action are unclear. Thus, this review comprehensively represents the relative abundance of micro/nanoplastics in different soil types and their toxicities in plants with insights into their partitioning to different soil matrices, uptake mechanisms, and the mode of action. Partitioning of micro/nanoplastics to different soil matrices (like-soil particles, naturally occurring soil organic matters, pore waters and soil fauna) could modify their bioavailability to plants. The small micro/nanoplastic particles can be taken up by roots through the apoplastic and symplastic pathways. In this regard, cellular endocytosis and aquaporin might play a significant role. The shape of the polymers can also regulate their uptake, and the polymers with spherical shapes are more easily absorbed by roots than the polymers with other shapes. Bioaccumulation of micro/nanoplastic induces oxidative stress, which, in turn, causes alterations of gene expressions and different metabolic pathways responsible for plant growth, biomass production and synthesis of secondary metabolites.

Functional interplay between plastic polymers and microbes: a comprehensive review.

Escalated production of plastic, their worldwide distribution and persistent nature finally results into their environmental accumulation causing severe threats to the ecological environment and biotic health. Thus, development of suitable measurements for environmental remediation of plastic may be an urgent issue in this plastic age. Some recent reviews have categorized the microbial species able to degrade different plastic polymers and the different factors effecting bio-degradation of plastic are poorly understood. This review comprehensively discusses bio-degradation of traditional and biodegradable plastic polymers both in natural and biological environment (gut microbes and fungi) to understand different factors regulating their degradation, and also shows how degradation of plastic polymers under abiotic factors influence subsequent biological degradation. Different physicochemical modifications like - breaking large polymers into small fragments by pre-treatment, functional groups enrichment, identifying potent microbial species (consortia) and engineering microbial enzymes might be crucial for bio-degradations of plastic. Effects of micro/nanoplastic and other chemical intermediates, formed during the bio-degradation of plastic, on species composition, abundance, growth, metabolism and enzymatic systems of microbes involved in the bio-degradation of plastic should be determined in future research.

Perspectives and challenges of micro/nanoplastics-induced toxicity with special reference to phytotoxicity.

Plastic pollution has become a global concern for ecosystem health and biodiversity conservation. Concentrations of plastics are manifold higher in the terrestrial system than the aquatic one. Micro/nanoplastics (M/NP) have the ability to alter soil enzymatic system, soil properties and also affect soil borne microorganisms and earthworms. Despite, the knowhow regarding modulatory effects of plastics are acquired from the study on aquatic system and reports on their phytotoxic potentials are limited. The presence of cell wall that could restrict M/NP invasion into plant roots might be the putative cause of this limitation. M/NP inhibit plant growth, seed germination and gene expression; and they also induce cytogenotoxicity by aggravating reactive oxygen species generation. Dynamic behavior of cell wall; the pores formed either by cell wall degrading enzymes or by plant-pathogen interactions or by mechanical injury might facilitate the entry of into roots M/NP. This review also provides a possible mechanism of large sized microplastics-induced phytotoxicity especially for those that cannot pass through cell wall pores. As M/NP affect soil microbial community and soil parameters, it is hypothesized that they could have the potential to affect N2 fixation and research should be conducted in this direction. Reports on M/NP-induced toxicity mainly focused only on one polymer type (polystyrene) in spite of the toxicological relevancies of other polymer types like polyethylene, polypropylene etc. So, the assessment of phytotoxic potential of M/NP should be done using other plastic polymers in real environment as they are known to intract with other environmental stressors as well as can alter the the soil-microbe-plant interaction.

Analysing progress of sustainable development goal 6 in India: Past, present, and future.

The attainment of sustainable development in water and sanitation i.e. Sustainable Development Goal 6 (SDG 6) requires a comprehensive monitoring and knowledge base of social and economic water needs at national scale in such a way that should not cause negative environmental manifestations from regional to global scale. India holds a key position among developing economies with a complex interconnected web of fast-growing population, coupled with biophysical stress, social deprivation and economic inequality related to utilization, availability and access to water resources and sanitation facilities. This study addresses some of these challenges related to monitoring and implementation of the targets of the UN SDG 6 in India. Acknowledging the contribution of society and economy in sustainability paradigm, here we have chosen 28 indicators (clustered into 11 dimensions) under two major groups, concerning biophysical and social development aspects of water and sanitation. We have shown declining level of per capita biophysical water resource and slow to rapidly developing societal indicators related to SDG 6 in India. From past trends, we have calculated probable scenario of biophysical consumption of India up to 2050. We have also analysed the interrelationship of water and sanitation (SDG 6) with health (SDG 3) and end to poverty (SDG 1) in India. These show a positive impact of increasing per capita GDP on improving sanitation related indicators, which in turn positively influence to reduce water and sanitation related diseases, especially in children and aged population. This cumulative assessment framework contributes a tool to prioritize water resource appropriation and assessment of sanitation, management response and policy implementations to national level inclusive sustainability of water and sanitation sector, keeping in mind the societal and economic development scenario in India.

Seasonal ovarian development in relation to the gonadotropins, steroids, aromatase and steroidogenic factor 1 (SF-1) in the banded gourami, Trichogaster fasciata.

The endocrine regulation of gonadal development and annual variation of key sex steroids is the basic knowledge to understand the reproductive cycle of teleost fish. Present study was aimed to investigate the levels of gonadotropins in relation to the follicular development and plasma steroids during the reproductive cycle of female Trichogaster fasciata. Female fish were sampled and ovarian development is described histologically throughout the year in relation to the seasonal variations of gonadosomatic index (GSI); follicle stimulating hormone (FSH) and luteinizing hormone (LH); three key steroids for folliculogenesis and maturation i.e. testosterone (T), 17ß-estradiol (E2) and 17α20ßdihydroxy4pregnen3one (17,20ß-P). Relatively higher level of FSH was observed till the ovary reaches in late vitellogenic stage confirms that FSH regulates the early folliculogenesis of the ovary, whereas LH peak was observed in the postvitellogenic stage, which indicates that maturation and ovulation were controlled by LH. Seasonal steroid profiles show that both T and E2 reach its maximum level prior to the 17,20ß-P which attain its peak value in the month of August. Thus, single peak values of LH and 17,20ß-P coinciding with GSI peak, clearly indicates that T. fasciata breeds only once in a year. Furthermore, to elucidate the molecular basis of the reproductive cycle, this study analyzes the other key factors of ovarian function such as cyp19a1a gene expression, aromatase activity and SF-1 localization throughout the year. cyp19a1a gene expression and the aromatase activity were highest in vitellogenic stages indicate that relatively higher E2 production in this stage is regulated by FSH. Immunohistochemical localizations of aromatase and SF-1 in the cellular layer of oocytes demonstrated that aromatase is FSH-dependent and SF-1 could be regulated by both FSH and LH as relatively higher amount of aromatase was localized in the vitellogenic stage oocytes than the postvitellogenic and post germinal vesicle breakdown (post-GVBD) stages; whereas, high amount of SF-1 was observed in vitellogenic, postvitellogenic and post-GVBD stages. These data regarding the reproductive endocrinology of T. fasciata may be useful to understand the interaction between gonadotropins, steroids, aromatase and SF-1 in teleost fishes and may contribute to restoration of the ecologically important fish through artificial reproduction.

Molecular mechanism of mercury-induced reproductive impairments in banded gourami, Trichogaster fasciata.

Mercury is one of the key pollutants responsible for the degradation of natural aquatic ecosystems. Among the different forms of mercury that exist in the environment, mercuric chloride (HgCl2) is the dominant pollutant for freshwater environments as it is used as an ingredient in antiseptics, disinfectants and preservatives, insecticides, batteries and in metallurgical and photographic operations. Pollutant may exert their action on organisms or populations by affecting their normal endocrine function as well as reproduction. Thus, the present study tried to understand the effect of mercuric chloride (HgCl2) on reproductive function and to decipher the molecular mechanism of Hg-induced reproductive impairments of female Trichogaster fasciata. Both in vivo and in vitro experiments were performed by using ecologically relevant doses of HgCl2 and the resulting effects on follicular development, steroidogenic potentiality, aromatase activity, aromatase gene expression and steroidogenic factor-1 (SF-1) expression pattern were analysed. In vivo exposure to HgCl2 caused reproductive impairments as shown by the inhibitory role of HgCl2 on follicular development, steroid biosynthesis and SF-1 activity. In vitro experiments revealed that aromatase activity, steroidogenesis, aromatase and SF-1 expression were blocked by HgCl2. The results obtained from this study contribute to understand the molecular mechanism of HgCl2-induced reproductive impairment of T. fasciata.

In vitro induction of oocyte maturation and steroidogenesis by gonadotropins, insulin, calcitonin and growth factor in an estuarine flat head grey mullet, Mugil cephalus L.

In this article, an in vitro investigation was carried out to ascertain the roles of hormones and growth factor in the inductions of oocyte maturation and steroidogenesis of the postvitellogenic follicles in an Indian estuarine grey mullet, Mugil cephalus L. Oocyte maturation was evaluated by scoring the germinal vesicle breakdown (GVBD) percent of the postvitellogenic follicles. All the sex [17α,20ß-dihydroxy-4-pregnane-3-one (DHP), estradiol 17ß (E2), progesterone (P), 17α-OH progesterone (17-OH-P) and testosterone] and other [bovine-insulin and salmon-calcitonin, human chorionic gonadotropin (hCG), luteinizing hormone (LH) or hCG+DHP] hormones and insulin-like growth factor-I (IGF-I) significantly increased GVBD% in 9 h culture. DHP had a maximum effect (75 %) compared to other effectors. Some effectors (hCG: 82.14 %, LH: 78.94 %, hCG plus DHP: 81.81 %, E2: 80 % and IGF-I: 74.19 %) including DHP (79 %) further increased GVBD% in 15-h culture. All the hormones (except DHP) and IGF-I increased DHP, E2 and testosterone productions by the postvitellogenic ovarian follicles in vitro. DHP and testosterone productions were increased with the increase of incubation time from 9 h through 15 h. E2 production was not further increased beyond 12 h. DHP production was highest by hCG compared to other effectors. The hCG of all the test compounds was most effective in both the induction of GVBD% and steroid production. DHP is the most potent inducer of oocyte maturation in Indian estuarine flat head grey mullet. Involvement of estrogen in mullet oocyte maturation is indicated. hCG, like DHP, is equally potent and induces oocyte maturation via DHP production in vitro. hCG with DHP has synergistic action on oocyte maturation in mullet ovary. Interplay of several hormones (hCG, LH, and probably E2 and testosterone) and IGF-I on oocyte maturation is suggested in the mullet.

Toxicological effects of nodularin on the reproductive endocrine system of female zebrafish (Danio rerio).

Nodularin is a potent cyanotoxin that has been detected in aquatic environments as well as in the body of aquatic organisms throughout the world, but its effects on the reproductive system are yet to be explored. The present study investigated the toxic effects of environmentally relevant concentrations of nodularin on the reproductive endocrine system of female zebrafish (Danio rerio). After exposure to nodularin for 14 days, decreased gonadosomatic Index (GSI), germinal vesicle breakdown (GVBD), and decreased level of follicle-stimulating hormone (FSH), luteinizing hormone (LH), 17ß-estradiol (E2) level and increased testosterone (T) content in female zebrafish suggested that nodularin may disrupt both oocyte growth and maturation. In support of this data, alteration in different marker gene expression on the hypothalamic-pituitary-gonadal-liver (HPGL) axis was observed. Transcriptional levels of genes related to steroidogenesis including cytochrome P450 aromatase (cyp19a1a) in the ovary and primary vitellogenin genes (vtg1, vtg2, and vtg3) in the liver were down-regulated and marker genes for oxidative stress (sod, cat, and gpx) were up-regulated on HPGL axis. These findings revealed for the first time that nodularin is a potent endocrine-disrupting compound posing oxidative stress and causes reproductive endocrine toxicity in female zebrafish, emphasizing the importance of assessing its environmental risks.

Evaluating the potential of daily intake of polystyrene microplastics via drinking water in inducing PCOS and its ovarian fibrosis progression using female zebrafish.

Polystyrene microplastics, extensively considered endocrine disrupting chemicals, disturb the reproductive system of living organisms. Polycystic ovary syndrome (PCOS), the reproductive endocrinopathy, is longstanding concern due to its eternal impacts as reproductive disorder and infertility. Despite several reports in reproductive and endocrine toxicity, there is inadequate literature regarding the daily intake of polystyrene-microplastics via drinking water in causing PCOS and leading to ovarian fibrosis in long-term. The present study investigated whether daily consumption of polystyrene-microplastics at doses equivalent to human exposure can cause PCOS and progress to ovarian fibrosis, using female zebrafish as model. Resembling letrozole-PCOS zebrafish model, daily intake of polystyrene-microplastics displayed hallmark PCOS pathophysiology; like excess body weight and %Gonadosomatic index, decreased Follicle Stimulating Hormone and ß-estradiol, increased Luteinising Hormone, brain and ovarian Testosterone (39.3% and 75% respectively). Correspondingly, ovarian histology revealed more developing (stage I and II) oocytes and less mature oocytes alongwith cystic lesions; like follicular membrane disorganization, zona pellucida invagination, theca hypertrophy, basophilic granular accumulation and oocyte buddings. Lipid deposition in intestinal and ovarian tissues was evidenced and increased fasting blood glucose manifesting insulin resistance. The expression of PCOS biomarkers (tox3, dennd1a, fem1a) was significantly disturbed. Polystyrene microplastics played vital role in inducing PCOS further enhancing oxidative stress, which positively influences inflammation and aggravate ovarian mitophagy, shedding light on its ability to harshen PCOS into ovarian fibrosis, which is characterized by collagen deposition and upregulation of pro-fibrogenic biomarker genes. These findings illustrate the potential of daily microplastics intake via drinking water in triggering PCOS and its progression to ovarian fibrosis.

Luteinizing hormone stimulation of in vitro ovulation in brook trout (Salvelinus fontinalis) involves follicle contraction and activation of proteolytic genes.

Luteinizing hormone (LH) is an essential hormone for the stimulation of the ovulatory process in vertebrates. However, little is known in fish regarding the different mechanisms induced by LH during ovulation that facilitate the rupture of the follicle wall and the subsequent expulsion of the mature oocyte. In this study, the effects of salmon LH (sLH) on in vitro ovulation were investigated in brook trout (Salvelinus fontinalis) isolated follicles. sLH significantly stimulated in vitro ovulation and contraction of brook trout preovulatory follicles. In order to investigate the possible involvement of proteolytic events in the ovulatory action of LH, the expression of genes known to have a crucial role in the degradation of follicle wall structure was examined. Our results show that sLH clearly stimulated the mRNA expression levels of matrix metalloproteinases (MMPs; including mmp2 and mmp19) and other enzymes with proteolytic action during ovulation, such as a disintegrin and metalloproteinase with thrombospondin-like motifs 1 (adamts1) and plasminogen (plg), in brook trout preovulatory follicles. In addition, the expression of mmp2, adamts1 and plg increased in brook trout follicles during the progression of LH-induced ovulation. Interestingly, the expression of tissue inhibitor of matrix metalloproteinase 2 (timp2), a known regulator of MMP2 activity, paralleled that of mmp2, suggesting the existence of a controlled mechanism of MMP2 action. Therefore, the known increase in proteolytic activity during ovulation in fish could be the result of the stimulation of the expression of proteolytic enzymes by LH in preovulatory follicles. We propose that LH may stimulate ovulation in brook trout follicles by stimulating proteolysis of the follicle wall and by stimulating follicle contraction.

Involvement of PI3 kinase and MAP kinase in IGF-I and insulin-induced ovarian steroidogenesis in common carp Cyprinus carpio.

Previously, we observed that in vitro steroidogenesis in intact ovarian follicles of common carp Cyprinus carpio can alone be induced by recombinant human insulin-like growth factor (IGF-I) and bovine insulin (b-insulin) and this induction was gonadotropin-independent. To investigate early signal transduction components involved in this process, the possible role of phosphatidylinositol 3-kinase (PI3 kinase) during ovarian steroidogenesis was examined. IGF-I and b-insulin induced testosterone and 17ß-estradiol production in carp ovarian theca and granulosa cells in short-term coincubation and this induction was significantly inhibited by Wortmannin and LY294002, two mechanistically different specific inhibitors of PI3 kinase. IGF-I and b-insulin were shown to activate PI3 kinase from 30 min onwards with a maximum at 90 min. In this study, we found the involvement of mitogen-activated protein kinase (MAP kinase) in the regulation of IGF-I- and b-insulin-induced steroidogenesis in carp ovary. An antagonist of mitogen-activated protein kinase kinase1/2 (MEK1/2) markedly attenuated IGF-I- and b-insulin-induced steroid production. Cells treated with IGF-I and b-insulin stimulated ERK1/2-dependent phosphorylation of extracellular signal regulated protein kinase1/2 (ERKs1/2) in a time-dependent manner, which was significantly attenuated in presence of MEK1/2 inhibitor. PI3 kinase inhibitors strongly attenuated phosphorylation and activation of MAP kinase, which was increased during IGF-I and b-insulin-induced steroidogenesis. Taken together, these results suggest that PI3 kinase is an initial component of the signal transduction pathway which precedes the MAP kinase during IGF-I- and b-insulin-induced steroidogenesis in C. carpio ovarian follicles.

High doses of nano-polystyrene aggravate the oxidative stress, DNA damage, and the cell death in onions.

Plastic pollution has been reported to negatively impact global biodiversity and ecosystem health. However, the molecular mechanisms of nano-plastics in plants are unidentified, especially their negative impacts on genomic stability. This study for the first time showed that nano-polystyrene leads to cell death in plants by subjugating the cellular antioxidant defence mechanisms through the aggravated production of ROS, which in turn could induce the DNA damage impairing the genetic regulation of the corresponding DNA repair pathway. To validate the proposed hypothesis, the DNA damage potential of nano-polystyrene and the expression levels of key genetic regulators of the DNA damage repair pathway (such as - CYCA/B, CDKA, SOG1, MYB transcription factors, and RAD51) have been assessed in onion roots after 72 h exposure with three ecologically relevant concentrations (25, 50, and 100 µg ml-1) of 100 nm nano-polystyrene. In addition, imbalance in redox homeostasis (oxidative stress), cell viability, and nuclear aberrations such as - the frequency of micronucleus and bi-nucleate cells that are directly linked to the DNA damages have been checked to point out the cause and effect of nano-polystyrene-induced DNA damage. Results showed a significant increase in oxidative stress in each treatment concentrations of nano-polystyrene. However, ROS generated at 100 µg ml-1 nano-polystyrene dose subdues the antioxidant defence system and induces cell death. These observations may be ascribed to the accumulation damaged DNA and the down-regulation of repair pathway-associated genes, as observed in this treatment group. Conversely, the observed DNA damage and the reduced expressions of genes would be a mere consequence of reduced cellular viability.

Melatonin mediated activation of MAP kinase pathway may reduce DNA damage stress in plants: A review.

Melatonin is an important biomolecule found in diverse groups of organisms. Under different abiotic stresses, the synthesis of melatonin is markedly increased suggesting pivotal roles of melatonin in plants enduring stresses. Being an endogenous signaling molecule with antioxidant activity, melatonin alters many physiological responses and is found to be involved in regulating DNA damage responses. However, the molecular mechanisms of melatonin in response to DNA damage have not yet been studied. The present review aims to provide insights into the molecular mechanisms of melatonin in response to DNA damage in plants. We propose that the MAP kinase pathway is involved in regulating melatonin dependent response of plants under DNA damage stress. Where melatonin might activate MAPK via H2 O2 or Ca2+ dependent pathways. The activated MAPK in turn might phosphorylate and activate SOG1 and repressor type MYBs to mitigate DNA damage under abiotic stress.

Toxicological impacts of nanopolystyrene on zebrafish oocyte with insight into the mechanism of action: An expression-based analysis.

Many studies have investigated the negative impacts of microplastics on teleost fishes with very little or no evidence of their mechanism of action. This scenario entreats us to investigate the toxicities of nanopolystyrene in zebrafish oocyte with emphasis on the mechanism of action. In the present study, the cellular levels of mRNA transcripts of different genetic markers (such as: sod, gpx, nrf2, inos, ucp2, and atp6 (redox-sensitive markers); nfkß, tnfα, il-10, ikß, gdf9, and bmp15 (immune markers); gadd45, rad51, p53 and bcl2 (DNA damage and apoptotic)) have been quantified by real-time PCR after 6 h of incubation of isolated oocyte with different doses of nanopolystyrene viz. P0 (control i.e. no polystyrene in culture medium), P1 (100 ng/ml), and P2 (400 ng/ml). Results showed that both the treatment concentrations of nanopolystyrene induce oxidative stress with % DPPH = 30.75, 31.61, and 32.43% for P0, P1, and P2, respectively. Increase in oxidative stress in oocytes with increasing doses of nanopolystyrene was also observed in TBARS assay with MDA content 0.12 and 0.21 µM for P1 and P2, respectively as compaired to the control 0.08 µM. This increased oxidative stress can regulate the expression pattern (upregulation/downregulation) of selected genes leading to different toxic effects like - oxidative stress, immunotoxicity, and apoptosis in oocytes, which suggests the impairment of reproductive functions by nanopolystyrene.

Co-occurrence of co-contaminants: Cyanotoxins and microplastics, in soil system and their health impacts on plant - A comprehensive review.

Cyanotoxins (CTX) and micro/nanoplastics (M/NP) are ubiquitously distributed in every environmental compartment. But the distribution, abundance and associated ecological risks of CTX are still poorly understood in soil system. On the other hand, M/NP could serve as vectors for persistent organic/inorganic pollutants in the natural environment through the sorption of pollutants onto them. Thus, co-occurrence of CTX and M/NP in soils suggests the sorption of CTX onto M/NP. So, major aim of this review is to understand the relevance of CTX and M/NP in soils as co-contaminants, possible interactions between them and ecological risks of CTX in terms of phytotoxicity. In this study, we comprehensively discuss different sources and fate of CTX and the sorption of CTX onto M/NP in soil system, considering the partition coefficient of different phases of soil and mass balance. Phytotoxicity of CTX, CTX mixture and co-contaminants has also been discussed with insights on the mechanism of action. This study indicates the need for the evaluation of sorption between co-contaminants, especially CTX and M/NP, and their phytotoxicity assessment using environmentally relevant concentrations.

Critical analysis of biophysicochemical parameters for qualitative improvement of phytogenic nanoparticles.

Conventional chemical approaches for synthesizing nanoparticles (NPs) may restrict their applicability as they are not eco-friendly, energetically efficient and often involve toxic reducing/capping agents; but phytonanotechnology enabled the synthesis of safe, inexpensive, highly biocompatible NPs. In this regard, thorough understanding of green components and the modulatory effects of different reaction conditions on the physicochemical parameters of green synthesized NPs would be a prerequisite, which is not depicted elsewhere. This review critically analyzes the relevant reaction conditions from their mechanistic viewpoints in plant-based synthesis of NPs arising fundamental issues which need to be determined carefully. The size, stability and surface chemistry of phytogenic NPs may be fabricated as a function of multiple interconnected reaction parameters and the plant species used. The therapeutic potential of phytogenic NPs may depend on the plant species used; and so the meticulous understanding of physicochemical parameters and the family wise shorting of elite plant species may potentially benefit the theranostic future of plant-based NPs.

Toxic effects of cyanotoxins in teleost fish: A comprehensive review.

The phenomenon of eutrophication leads to the global occurrence of algal blooms. Cyanotoxins as produced by many cyanobacterial species can lead to detrimental effects to the biome due to their stability and potential biomagnification along food webs. Therefore, understanding of the potential risks these toxins pose to the most susceptible organisms is an important prerequisite for ecological risks assessment of cyanobacteria blooms. Fishes are an important component of aquatic ecosystems that are prone to direct exposure to cyanotoxins. However, relatively few investigations have focused on measuring the toxic potentials of cyanotoxins in teleost fishes. This review comprehensively describes the major toxicological impacts (such as hepatotoxicity, neurotoxicity, immune toxicity, reproductive toxicity and cytogenotoxicity) of commonly occurring cyanotoxins in teleost fishes. The present work encompasses recent research progresses with special emphasis on the basic molecular mechanisms by which different cyanotoxins impose their toxicities in teleost fishes. The major research areas, which need to be focused on in future scientific investigations, have also been highlighted. Protein kinase inhibition, transcriptional dysregulation, disruption of redox homeostasis and the induction of apoptotic pathways appear to be the key drivers of the toxicological effects of cyanotoxins in fish. Analyses also showed that the impacts of cyanotoxins on specific reproductive processes are relatively less described in teleosts in comparison to mammalian systems. In fact, as compared to other toxicological effects of cyanotoxins, their reproductive toxicity (such as impacts on oocyte development, maturation and their hormonal regulation) is poorly understood in fish, and thus requires further studies. Furthermore, additonal studies characterizing the molecular mechanisms responsible for the cellular uptake of cyanotoxins need to be investigated.

Interaction of plastic particles with heavy metals and the resulting toxicological impacts: a review.

Interactions of plastic particles with different organic/inorganic pollutants including heavy metals impact their ecotoxicological potential, and proper understanding in this regard is important for their ecological risk assessment. However, many studies have reported the interactions between micro-/nanoplastics (MNPs) and heavy metals (HMs), but the most prevalent interactive forces and factors monitoring their interactions are still not clear. So, the present review represents the mechanisms of interactions with special emphasis on major interactive forces and biophysicochemical and environmental factors influencing trace element's adsorption onto the surface of MNPs. Electrostatic interaction and pore-filling mechanism can best explain the HMs adsorption to MNPs. A number of biophysicochemical factors (such as biofilm, size, crystallinity, and surface charge) and environmental factors (such as pH, salt, and temperature) act together for mediating interactions and ecotoxicities of MNPs and HMs in the real environment. From a toxicological point of view, the synergistic mode of action may be more active in animals, whereas the antagonistic activity may be prevalent in plants. Besides polymer density, biofilm formation and agglomeration property of MNPs can control the vertical distribution of MNPs along the water column. Finally, the ecotoxicological potential of MNPs in the natural environment can be considered as a function of spatiotemporal variation in abiotic (including MNPs and heavy metals) and biotic components. This review will be helpful in the detail understanding of ecotoxicological risk assessment of MNPs in relation to their interaction with heavy metals.

Functions of interleukin-6 in ovulation of female climbing perch, Anabas testudineus.

In mammals, interleukin 6 (IL-6) has an important function during ovulation, however, the functions of IL-6 in fish have not been elucidated. In the present study, there was quantification of de novo synthesis of ovarian IL-6 and tumor necrosis factor-alpha (TNFα) in control and hCG-treated fish and results were compared with those from an in vitro study where there was evaluation of the regulatory functions of gonadotropins and TNFα of IL-6 secretions. Relatively greater concentrations of ovarian IL-6 at the post-GVBD (post-germinal vesicle breakdown) stage indicates IL-6 modulates ovulatory processes. The hCG-induced increase in relative abundance of IL-6 (in vitro) mRNA transcript and secretion from the ovary were attenuated when there was administration of the inhibitor of TNFα secreting enzyme, TAPI-I, which indicates TNFα modulates IL-6 secretion. Treatments with IL-6 induced a marked increase in ovulation rate in vitro when there was induction of activating matrix metalloproteinase (MMP). Furthermore, treatment with IL-6 resulted in production of prostaglandin as indicated by the IL-6 induced increase in the abundance of ptgs2 mRNA transcript in the ovary of Anabas testudineus. Furthermore, results indicate the source of IL-6 in the ovary is the granulosa cells with secretion of IL-6 being induced by the additions of hCG and TNFα in the medium. There was also an IL-6-induced increase in abundance of receptors (IL-6 Rα and gp130) to which it binds indicating IL-6 autoregulates this population of receptors. Results from this study, for the first time, elucidate the reproductive functions of IL-6 in a teleost fish.

Analysis of Youths' Perspective in India On and During the Pandemic of COVID-19.

Objective: In this COVID-19 pandemic, there are not many sound studies focusing on the extensive socioeconomic impact ushered in with this disaster. This work aims to understand the thought of the youth, their opinions and understanding of various aspects of the COVID-19 pandemic. Methodology: Using a combined qualitative-quantitative approach, Q-method, we tried to assess people's discernment from different perspectives. This was done through a questionnaire survey method during the national-level lockdown 1.0 in India. Results: We have differentiated the perceptions of youth respondents into seven factors, including six subdimensions, on COVID-19 pandemic (viz., science, society, environment, economy, politics, and religion). The choices and opinions have been segregated into two major groups: quantitative and qualitative. Conclusion: This work yielded a firsthand ground-level insight into the comprehensive yet diverse responses from youths regarding the COVID-19 pandemic in India. There are various topics that arise from this study, for example, misinformation, misinterpretation of science, dubious nature of faith in governance and policy, turbid understanding of strategy, polarization of opinion, and so forth. Following this work of identification, the next steps would be to understand how to mitigate the problems toward betterment in the COVID-19 pandemic situation or similar widespread crisis events in the foreseeable future.