RESUMO
To investigate the characteristics of rhesus monkey embryonic stem cells and to promote their clinical application, the differentiation and proliferation of rosettes neural stem cells from GFP marked rhesus monkey embryonic stem cells were studied The results showed that: 1) A stable and high-efficient neural differentiation system was established. More than 95% of the embryonic stem cells were differentiated into neural stem cells on the 12(th) days of differentiation; 2) the rosettes neural stem cells differentiated from the rhesus monkey embryonic stem cells could maintain their rosettes-shape by proliferating with bFGF/EGF; 3) the neural stem cells could differentiate into neurons after transplanted into the rhesus monkey brain. In conclusion, the rosettes neural stem cells differentiated from rhesus monkey embryonic stem cells could maintain their characteristics after proliferation with bFGF/EGF and they could survive and differentiate into neurons after transplanted into the rhesus monkey brain, which strongly supports the clinical application of neural stem cells in the future.
Assuntos
Diferenciação Celular , Proliferação de Células , Modelos Animais de Doenças , Células-Tronco Embrionárias/citologia , Células-Tronco Embrionárias/transplante , Macaca mulatta , Doenças do Sistema Nervoso/terapia , Animais , Células Cultivadas , Humanos , Macaca mulatta/fisiologia , Sistema Nervoso/citologia , Doenças do Sistema Nervoso/cirurgia , Neurônios/citologia , Transplante HomólogoRESUMO
To perform electrophysiological recording and other investigations on transplanted neural cells in vivo, we used mechanical damage to establish a special traumatic brain injury model that could distinguish transplanted cells from host cells. The morphology of the trauma-induced holes in the cortex of the rat brain was regular. The model was stable and repeatable. Neural stem cells were transplanted into the trauma-induced hole, and were able to survive for a long time. Most of the transplanted cells differentiated into neurons, and only a small amount turned into glia cells. There was a clear boundary between the host cells and the transplanted cells. Single cell electrophysiological recording on transplanted neural cells were detected in vivo. This study established a stable and repeatable traumatic brain injury model, which could be used to conduct in vivo electrophysiological recording research on transplanted neural cells.
Assuntos
Lesões Encefálicas/terapia , Neurônios/transplante , Animais , Lesões Encefálicas/fisiopatologia , Células Cultivadas , Modelos Animais de Doenças , Fenômenos Eletrofisiológicos , Feminino , Humanos , Masculino , Neurônios/química , Neurônios/fisiologia , Ratos , Ratos Sprague-Dawley , Transplante de Células-Tronco , Células-Tronco/química , Células-Tronco/fisiologia , TransplantesRESUMO
In the present study, the differences between addictive memory and ordinary memory were investigated by morphine-induced and food-induced conditioned place preference (CPP) in adolescent and adult mice. The result showed that: 1) morphine-induced CPP could be established in adult mice but not in adolescent mice. 2) food-induced CPP could be established both in adolescent and adult mice. This study indicated that between adolescent and adult mice, there was no difference in ordinary memory, but for addictive memory, they were quite different. Our data suggested that the development of addictive memory and ordinary memory system in mouse brain might not be paralleled.