Phytoremediation of nickel and zinc using Jatropha curcas and Pongamia pinnata from the soils contaminated by municipal solid wastes and paper mill wastes.

The primary source of soil pollution is a complex mixture of numerous inorganic and organic compounds (including chlorinated compounds, nutrients, and heavy metals, etc.). The presence of all of these compounds makes remediation and cleanup difficult. In this study, the phytoremediation ability of Jatropha curcas and Pongamia pinnata was tested to remove nickel (Ni) and Zinc (Zn) from paper mill and municipal landfill contaminated soils, to understand the uptake potential and to estimate the accumulation pattern of Ni and Zn in the vegetative parts of the plant. The experiments were carried out in pots (3 kg capacity) and the different combinations of soil were made by mixing the contaminated soil with a reference soil (forest soil) as T0, T25, T50, T75 and T100. The plant biomass, chlorophyll content, proline, nitrate reductase activity and metal removal efficiency (%)were determined after 120 DAS (i.e., the days after sowing). The results of the study showed that with increasing metal stress, there is a reduction in the above-ground biomass content in both the plant species with a slightly less impact on the root biomass. Over a period of 4 months, J. curcas and P. pinnata removed 82-86% and 93-90% Ni, respectively. The removal of Zn was significantly less as compared to Ni as most of the Zn remained in the belowground part (roots) and in the soil. Besides, the phytostabilization capacities of the plants were calculated on the basis of their tolerance index (TI), bioaccumulation factor (BAF) and translocation factor (TF). The low BAF and TF values with increasing heavy metals (HMs) content indicates its higher phytostabilization capacity in the root and rhizospheric region as compared to phytoaccumulation.

Optimization of laccase enzyme extraction from spent mushroom waste of Pleurotus florida through ANN-PSO modeling: An ecofriendly and economical approach.

The cardinal focus of this study is to optimize the best reaction conditions for maximizing laccase activity from spent mushroom waste (SMW) of Pleurotus florida. Optimization process parameters were studied by the modeling techniques, artificial neural networking (ANN) embedded in particle swarm optimization (PSO), and response surface model (RSM). The best topology of ANN-PSO architecture was obtained on 4-10-1. The R2, IOA, MSE, and MAE values of the ANN model were obtained as 0.98785, 0.9939, 0.0023, and 0.0251 while, that of the RSM model were obtained as 0.74290, 0.9210, 0.0244, and 0.1110 respectively. The higher values of R2, IOA, and lower values of MSE and MAE of the ANN-PSO model depict that ANN-PSO outperformed compared to RSM and also verified the effectiveness of the ANN-PSO model. The ANN-PSO model performance demonstrates the robustness of the technique in optimizing laccase activity in SMW of P. florida. The optimization results revealed that pH 4.5, time 3 h, solid: solution ratio 1:5, and ABTS concentration of 1 mM was optimal for achieving maximum laccase activity at temperature 30 °C. The enzymatic activity of crude laccase enzyme was obtained as 1.185 U ml-1 without loss of enzyme activity. Additionally, crude laccase enzyme was 1.74 fold partially purified, and 83.54% of the enzyme was yielded. Out of all the independent process variables, ABTS and pH had an influence on laccase activity. Therefore, we anticipate that the findings of this investigation will reduce the ambiguity in maximizing laccase activity and ease the screening process. This study also highlights the comparative cost evaluation of crude laccase enzyme extracted from P. florida and commercial enzymes. There is a great potential for the utilization of the laccase enzyme extracted from SMW and using it for the degradation of recalcitrant micropollutants. Thus, SMW promises a cost-effective and sustainable approach leading towards circular economy.

Decoding the complete chloroplast genome of Cissus quadrangularis: insights into molecular structure, comparative genome analysis and mining of mutational hotspot regions.

Cissus quadrangularis L., a member of the Vitaceae family, is an important medicinal plant with widespread application in Indian traditional medicines. C. quadrangularis L. whole chloroplast genome of 160,404 bp was assembled using a genome skimming approach from the whole genome library. The assembled chloroplast genome contained a large single-copy region (88,987 bp), a small single-copy region (18,621 bp), and pairs of inverted repeat regions (26,398 bp). It also comprised 133 genes, including 37 tRNAs, eight rRNAs, and 88 protein-coding genes. Aside from that, we annotated three genes atpH, petB, and psbL, as well as one duplicated copy of the ycf1 gene in C. quadrangularis L. that had previously been missing from the annotation of compared Cissus chloroplast genomes. Five divergent hotspot regions such as petA_psbJ (0.1237), rps16_trnQ-UUG (0.0913), psbC_trnS-UGA (0.0847), rps15_ycf1 (0.0788), and rps2_rpoC2 (0.0788) were identified in the investigation that could aid in future species discrimination. Surprisingly, we found the overlapping genes ycf1 and ndhF on the IRb/SSC junction, rarely seen in angiosperms. The results of the phylogenetic study showed that the genomes of the Cissus species under study formed a single distinct clade. The detailed annotations given in this study could be useful in the future for genome annotations of Cissus species. The current findings of the study have the potential to serve as a useful resource for future research in the field of population genetics and the evolutionary relationships in the Cissus genus. Supplementary Information: The online version contains supplementary material available at 10.1007/s12298-023-01312-w.

Synthesis and characterization of zinc derivatized 3, 5-dihydroxy 4', 7-dimethoxyflavone and its anti leishmaniasis activity against Leishmania donovani.

This study reports the synthesis and characterization of zinc derivatized 3,5-dihydroxy 4', 7- dimethoxyflavone (DHDM-Zn) compound for the development of new antileishmanial agents. The interaction studies of DHDM with zinc were carried out by UV spectra and fluorescence spectra analysis. Characterization of the complex was further accomplished by multi-spectroscopic techniques such as FTIR, Raman, HRMS, NMR, FESEM-EDX. The morphological and topographical studies of synthesized DHDM-Zn were carried out using FESEM with EDX. Further, it was demonstrated that DHDM-Zn exhibited an excellent in vitro antagonistic effect against the promastigote form of L. donovani. In addition, the possible mechanisms of promastigote L. donovani cell death, by involvement of derivatized compound in arrest of the cell cycle in the G1 phase and residual cell count reduction were investigated. Promastigote growth kinetics performed in the presence of the derivatized compound revealed a slow growth rate. The combination of growth kinetics and cell cycle analysis, made it possible to interpret and classify the cause of leishmanial cell death accurately. These results support that zinc derivatized complex (DHDM-Zn) might work as a lead compound for designing and developing a new antileishmanial drug.

Development of EST-SSR markers for Pongamia pinnata by transcriptome database mining: cross-species amplification and genetic diversity.

EST-SSR markers were developed from Pongamia pinnata transcriptome libraries. We have successfully utilised EST-SSRs to study the genetic diversity of Indian P. pinnata germplasms and transferability study on legume plants. P. pinnata is a non-edible oil, seed-bearing leguminous tree well known for its multipurpose benefits and acts as a potential source for medicine and biodiesel preparation. Moreover, the plant is not grazable by animal and wildly grown in different agro climatic condition of India. Recently, it is much used in reforestation and rehabilitation of marginal and coal mined land in different part of India. Due to increasing demand for cultivation, understanding of the genetic diversity is important parameter for further breeding and cultivation program. In this investigation, an attempt has been undertaken to develop novel EST-SSR markers by analyzing the assembled transcriptome from previously published Illumina libraries of P. pinnata, which is cross transferrable to legume plants. Twenty EST-SSR markers were developed from oil yielding and secondary metabolite biosynthesis genes. To our knowledge, this is the first EST-SSR marker based genetic diversity study on Indian P. pinnata germplasms. The genetic diversity parameter analysis of P. pinnata showed that the Gangetic plain and Eastern India are highly diverse compared to the Central Deccan and Western germplasms. The lowest genetic diversity in the Western region may be due to the pressure of lower precipitation, high-temperature stress and reduced groundwater availability. Nevertheless, the highest genetic diversity of Gangetic plain and Eastern India may be due to the higher groundwater availability, high precipitation, higher temperature fluctuations and growing by the side of glacier-fed river water. Thus, our study shows the evidence of natural selection on the genetic diversity of P. pinnata germplasms of the Indian subcontinent.

Parametric optimality of tray dried Musa balbisiana Colla blossom.

Musa balbisiana Colla blossom has enriched applications as a key constituent of dried vegetable formulations. With restricted prior art, the article addresses the optimality of tray drying characteristics of the blossom from both statistical design and drying kinetics perspective. The process variables in due course of optimization refer to moisture content, antioxidant activity and vitamin C for variation in drying time and temperature. Model fitness, analysis of variance based analysis and numerical optimization were considered during the statistical design of experiments. Drying kinetics involved fitness studies of alternate models, moisture diffusivity and process variable characteristics. Thereby, the sensitivity of both approaches to obtain optimal parameters associated with tray dried product have been targeted for a comparative assessment.

Assessment of genetic variation among wild Alpinia nigra (Zingiberaceae) population: an approach based on molecular phylogeny.

Genetic structure was evaluated among wild Alpinia nigra (Gaertn.) B.L. Burtt, populations. The information of genetic relatedness was developed using random amplified polymorphic DNA (RAPD), inter-simple sequence repeat (ISSR) and barcoding loci (plastid and mitochondrial). The order (high to low) of Shannon's information index (I) and Nei's gene diversity (h) from the populations was: "IIT Guwahati" > "Amingaon" > "Saraighat". Genetic diversity decreased and genetic differentiation increased among the three populations. We observed no isolation by distance thus lower amount of gene flow was observed. Narrow range of genetic distance among the three populations and appearance of two distinct clusters strengthened the geographical isolation in dendrogram and principal component analysis. No mutation among the three populations was observed for seven plastid loci and two mitochondrial tested suggesting the taxonomic homogeneity. The phylogeny based on nine barcoding loci supported our observation that individuals of IIT Guwahati were partially isolated from the outside populations. Our study will provide a backbone for developing strategies to resist habitat fragmentation of Zingiberaceous plants.

Does nature of livelihood regulate the urban community's vulnerability to climate change? Guwahati city, a case study from North East India.

There is dearth of studies on climate change based vulnerabilities of the urban people. It has been a matter of widespread debate whether nature of livelihood has any role to play in regulating the vulnerabilities of an individual. To find an answer, in a first ever attempt, this study tested three different approaches viz. Livelihood Vulnerability Index (LVI), LVI IPCC (Intergovernmental Panel on Climate Change) models and Climate Vulnerability Index (CVI) to estimate climate change vulnerability of urban communities from various livelihoods in Guwahati city, Assam, India. Guwahati is considered to be the gateway to the seven North Eastern states of India and therefore strategically very important for this region. A structured survey was conducted involving 200 stakeholders from various livelihood sectors viz. construction workers, perishable item sellers, farmers, taxi/auto driver/rickshaw puller/coolie, tea stall/fast food seller, gas cylinder deliverymen, street vendors/salespersons, traffic police/police, doctors and boatmen. Data was systematically aggregated and examined using the above-mentioned composite indices. The differential vulnerabilities were compared and results suggested that the farmers were the most vulnerable community by virtue of their high sensitivity towards health, economic losses, exacerbated by their poor adaptive capacity toward unpredictable climatic variations. Doctors were the least vulnerable owing to their higher levels of awareness and adaptive capacity. These results reiterated the importance of awareness and access to resources in regulating vulnerability. The vulnerability scores also revealed that LVI and Model II (M II) of LVI IPCC approaches were the two most suitable indices and could be used for comparative vulnerability analysis. These pragmatic approaches can be used to assess the community vulnerabilities and could stimulate robust Climate Smart Urban Planning (CSUP).

Comparative analysis of contextual bias around the translation initiation sites in plant genomes.

Nucleotide distribution around translation initiation site (TIS) is thought to play an important role in determining translation efficiency. Kozak in vertebrates and later Joshi et al. in plants identified context sequence having a key role in translation efficiency, but a great variation regarding this context sequence has been observed among different taxa. The present study aims to refine the context sequence around initiation codon in plants and addresses the sampling error problem by using complete genomes of 7 monocots and 7 dicots separately. Besides positions -3 and +4, significant conservation at -2 and +5 positions was also found and nucleotide bias at the latter two positions was shown to directly influence translation efficiency in the taxon studied. About 1.8% (monocots) and 2.4% (dicots) of the total sequences fit the context sequence from positions -3 to +5, which might be indicative of lower number of housekeeping genes in the transcriptome. A three base periodicity was observed in 5' UTR and CDS of monocots and only in CDS of dicots as confirmed against random occurrence and annotation errors. Deterministic enrichment of GCNAUGGC in monocots, AANAUGGC in dicots and GCNAUGGC in plants around TIS was also established (where AUG denotes the start codon), which can serve as an arbiter of putative TIS with efficient translation in plants.

3, 5-Dihydroxy 4', 7-dimethoxyflavone-DNA interaction study for nucleic acid detection and differential cell staining.

The present study is focused on application of a natural compound, 3, 5-dihydroxy 4', 7-dimethoxyflavone (DHDM) from a medicinal plant Alpinia nigra for nucleic acid detection and differential cell staining. DHDM was found to interact with nucleic acid and forms complex, which was investigated for various applications. It was successfully utilized to visualize plasmid, genomic, and ds-linear DNA in agarose gel electrophoresis without affecting the DNA mobility in the gel. Fluorescence of DHDM increased several fold upon binding to dsDNA. Photostability of the compound was assessed and showed photobleaching effect that decreased gradually over time. Application of the compound was further extended to differential cell staining. When observed in fluorescence microscope, DHDM stained the dead cells and differentiated them from live cells in the case of bacterial, yeast, and mammalian cells. Higher concentration of the compound was found to be less cytotoxic to cancerous cells. Nucleic acid staining dyes like Ethidium bromide (EtBr), Propidium iodide (PI), etc. are carcinogens and environmental pollutants and therefore DHDM a natural compound, is a major benefit and thus can serve as an alternative to the current dyes.

The whole chloroplast genome of Mesua ferrea: Insight into the dynamic pattern of evolution and its comparison with species from recently diverged families.

Mesua ferrea is an important source of timber, oil and herbal medicines. In the present investigation, we assembled the whole chloroplast genome of M. ferrea of size 161.4 kb. The genome contained 86 protein-coding genes, 38 tRNAs, 8 rRNA genes and exhibited a characteristic quadripartite structural orientation, with two inverted repeats (27,614 bp) separated by an LSC (88,746 bp) region and an SSC (27,614 bp) (17,470 bp). Interestingly, no gene loss was identified in the M. ferrea genome, contrary to what has been observed in other Clusioid species. We compared the chloroplast genome of M. ferrea with the chloroplast genome of Bonnetia and Garcinia belonging to Bonnetiaceae and Clusiaceae families. Overall, the compared genomes possess a similar synteny of gene order except for a small inversion in Garcinia species. M. ferrea has the largest chloroplast genome size in Clusioid clade owing to the lengthening of the LSC, IR, and non-coding regions. Substantial differences were observed in population of simple sequence repeats (SSRs) and RNA editing sites among the studied genomes. A comparative assessment of chloroplast genomes revealed five highly divergence regions: rpl32, trnS-GCU_trnG-UCC, petN-psbM, psbZ_trnG-GCC and ccsA_ndhD among the analyzed sequences. Phylogenetic analyses and sequence homology search indicate that M. ferrea is closely related to the Garcinia species.

Global transcriptome analysis reveals partial estrogen-like effects of karanjin in MCF-7 breast cancer cells.

Karanjin, an abundantly occurring furanoflavonoid in edible and non-edible legumes, exerts diverse biological effects in vivo, and in vitro. Its potential as an anticancer agent is gaining traction following recent demonstrations of its anti-proliferative, cell cycle inhibitory, and pro-apoptotic effects. However, the genomic correlates of these activities are not known. In the present study we delineated the transcriptomic footprint of 10 µM karanjin in MCF-7 breast cancer cells, using next generation sequencing technology (RNA-seq). We show that karanjin-modulated gene-expression repertoire is enriched in several hallmark gene sets, which include early estrogen-response, and G2/M checkpoint genes. Genes modulated by karanjin overlapped with those modulated by 1 nM 17ß-estradiol (E2), or 1 µM tamoxifen. The results suggest partial estrogen-like activity of karanjin, thereby presenting a caveat to its anticancer potential. Further investigations into its mechanisms of action are warranted to ascertain the true potential of karanjin in anticancer, or endocrine therapy.

A Review on Application of DNA Barcoding Technology for Rapid Molecular Diagnostics of Adulterants in Herbal Medicine.

The rapid molecular diagnostics of adulterants in herbal medicine using DNA barcoding forms the core of this meticulously detailed review, based on two decades of data. With 80% of the world's population using some form of herbal medicine, authentication, quality control, and detection of adulterants warrant DNA barcoding. A combined group of keywords were used for literature review using the PubMed, the ISI Web of Knowledge, Web of Science (WoS), and Google Scholar databases. All the papers (N = 210) returned by the search engines were downloaded and systematically analyzed. Detailed analysis of conventional DNA barcodes were based on retrieved sequences for internal transcribed spacer (ITS) (412,189), rbcL (251,598), matK (210,835), and trnH-psbA (141,846). The utility of databases such as The Barcode of Life Data System (BOLD), NCBI, GenBank, and Medicinal Materials DNA Barcode Database (MMDBD) has been critically examined for the identification of unknown species from known databases. The current review gives an overview of the ratio of adulterated to authentic drugs for some countries along with the state of the art technology currently being used in the identification of adulterated medicines. In this review, efforts were made to systematically analyze and arrange the research and reviews on the basis of technical progress. The review concludes with the future of DNA-based herbal medicine adulteration detection, forecasting the reliance on the metabarcoding technology. DNA barcoding technology for differentiating adulterated herbal medicine.

Bacterial strains found in the soils of a municipal solid waste dumping site facilitated phosphate solubilization along with cadmium remediation.

Phosphate solubilizing bacteria (PSB) that can withstand high cadmium (Cd) stress is a desired combination for bioremediation. This study evaluated the Cd bioremediation potential of four PSB strains isolated from the contaminated soils of a municipal solid waste (MSW) discarding site (Guwahati, India). PSB strains were cultured in Pikovskaya (PVK) media, which led to higher acid phosphatase (ACP) activity and the release of organic acid. Optical density (OD) measurements were performed to determine the growth pattern of PSB; furthermore, Cd uptake by PSB was evaluated using infrared spectroscopy (IR) and X-Ray Diffraction (XRD) analyses. The 16S rRNA taxonomic analysis revealed that all the four promising PSB strains belonged to either Bacillus sp. or Enterobacter sp. One strain (SM_SS8) demonstrated higher tolerance towards Cd (up to 100 mg L-1). Flow cytometry analysis revealed 70.92%, 46.93% and 20.4% viability of SM_SS8 in 10, 50 and 100 mg L-1, respectively in PVK media containing Cd. This study has therefore substantiated the bioremediation of Cd from polluted soil by the PSB isolates. Thus, experimental results revealed a potential combo benefit, phosphate solubilization along with Cd remediation.

Occurrence, geochemical fraction, ecological and health risk assessment of cadmium, copper and nickel in soils contaminated with municipal solid wastes.

Unscientific municipal solid waste (MSW) dumping provokes heavy metal (HM) associated ecological and human health hazards through heightened bioavailability and bioaccumulation. In this study, we focused on three important HMs Cadmium (Cd), Copper (Cu) and Nickel (Ni) and their geochemical fractions, to enable clutter free data management, analysis and interpretation. Stratified random soil sampling was carried out from twenty different locations around a Ramsar site (Deepor Beel) in Guwahati, India. The spatial concentration profiles of Cd, Cu and Ni were determined by data elicited from geochemical fractionation and the Geographic Information System (GIS). Ecological and health risks indices were used to evaluate the severity of soil pollution and assess the level of health risks. All the three HMs thus evaluated, conformed to the potential bioavailable category. Cd (54.59%) was associated mostly with the carbonate bound fraction (F3), while 25.53% of Cu and 40.60% Ni were associated with the exchangeable fraction (F2). Significant contamination levels and higher ecological risks posed by these metals were in the order Cd > Ni > Cu. Children were found to be more vulnerable towards Cd associated health risks whereas, Ni posed threats to both adults and children. Cu posed no risk to human health. Geochemical fractionation and different indices played a critical role in the integrated assessment of soil pollution, ecological and health risk assessment, and provided an empirical basis for the sustainable future planning and comprehensive adaptive management practices for MSW.

Therapeutic potential of labdane diterpene isolated from Alpinia nigra: detailed hemato-compatibility and antimicrobial studies.

(E)-labda-8(17), 12-diene-15,16-dial has been isolated from the seeds of Alpinia nigra that is unsuitable for oral administration and evident from in silico studies. The present investigation therefore deals with understanding the effect of this compound on RBCs for intravenous administration. No prominent hemolytic effect of compound at a concentration of ≤0.4 mg/ml was found whereas higher concentrations perforated RBC membrane. The molecule showed remarkable inhibitory potential against Gram negative bacteria (concentration ≥0.025 mg/ml) causing cell lysis. In case of pathogenic yeast Candida albicans although growth was inhibited (concentration ≥ 0.0025 mg/ml), growth kinetic study revealed that the diterpene significantly delayed fungal growth (concentration 0.005-0.020 mg/ml) by preventing substrate uptake and was able to extend its lag phase in a dose-dependent manner. This study tries to unveil the mechanism of action of this diterpene on microorganisms with differential cell wall compositions.

Deciphering the dynamics of glomalin and heavy metals in soils contaminated with hazardous municipal solid wastes.

Heavy metals (HMs) accumulation in the soils poses risks towards the environment and health. Glomalin related soil protein (GRSP) produced by arbuscular mycorrhizal fungi (AMF) has metal-sorption and soil aggregation properties and is critical in the survival of plants and AMF. For the first time, this study attempted to examine the GRSP mediated bio-stabilization of HMs in soils contaminated with municipal solid wastes (MSW). The content and interrelationship of GRSP and HMs, along with soil physicochemical properties were studied in 20 different soil samples from the dumping site. Higher amount of GRSP indicated potential bio-stabilization of HMs at some sites. GRSP exhibited weak positive correlation with essential (Zn, Cu) and toxic HMs (Cd, Ni). Cr and Mn were possibly sequestered in AMF structures and thus found to be negatively correlated with GRSP. The positive correlation observed between GRSP and soil nutrients like N, P and soil organic carbon (SOC) indicating potential of AMF-GRSP in sustaining soil health. Results revealed that AMF residing at contaminated sites produced higher amount of GRSP potentially to bio-stabilize the HMs, and reduce their bioavailability and also facilitate SOC sequestration.

Sustainable improvement of soil health utilizing biochar and arbuscular mycorrhizal fungi: A review.

Conservation of soil health and crop productivity is the central theme for sustainable agriculture practices. It is unrealistic to expect that the burgeoning crop production demands will be met by a soil ecosystem that is increasingly unhealthy and constrained. Therefore, the present review is focused on soil amendment techniques, using biochar in combination with arbuscular mycorrhizal fungi (AMF), which is an indispensable biotic component that maintains plant-soil continuum. Globally significant progress has been made in elucidating the physical and chemical properties of biochar; along with its role in carbon sequestration. Similarly, research advances on AMF include its evolutionary background, functions, and vital roles in the soil ecosystem. The present review deliberates on the premise that biochar and AMF have the potential to become cardinal to management of agro-ecosystems. The wider perspectives of various agronomical and environmental backgrounds are discussed. The present state of knowledge, different aspects and limitations of combined biochar and AMF applications (BC + AMF), mechanisms of interaction between biochar and AMF, effects on plant growth, challenges and future opportunities of BC + AMF applications are critically reviewed. Given the severely constrained nature of soil health, the roles of BC + AMF in agriculture, bioremediation and ecology have also been examined. In spite of the potential benefits, the functionality and dynamics of BC + AMF in soil are far from being fully elucidated.

Speciation, contamination, ecological and human health risks assessment of heavy metals in soils dumped with municipal solid wastes.

The main aim of this work is to assess the extent of soil contamination, potential ecological and health risks associated with the disposal of municipal solid waste (MSW) near a Ramsar site in Assam, India. Soil samples were collected and analysed for three heavy metals (HMs), namely, chromium (Cr), manganese (Mn) and zinc (Zn). The sources of HMs and their pollution levels were evaluated using different indices. The results demonstrated that Cr contamination was high near the metal scrap segregations unit within the dumping site, otherwise, the ecological risks associated with Zn and Mn were found to be low. The speciation of Cr and Zn were associated with the Fe-Mn oxide bound (F4) fraction, accounting 44.23% and 30.68%, respectively, whereas Mn (52.55%) was associated with the exchangeable fraction (F2). The fate and origin of HMs were assessed using mobility and enrichment factors and 16 out of the 20 sampling sites fell under the category of heavily polluted category for Cr, while others which were nearby the metal segregation units fell under the strongly to extremely polluted category. In few sites, significant enrichment was observed for Zn and minimal to moderate enrichment for Mn, respectively. Health risk assessment results indicated that Cr posed higher threat to human health through ingestion.

The Role of Transposable Elements in Pongamia Unigenes and Protein Diversity.

Pongamia pinnata (also called Millettia pinnata), a non-edible oil yielding tree, is well known for its multipurpose benefits and acts as a potential source for medicine and biodiesel preparation. Due to increase in demand for cultivation, understanding of genetic diversity is an important parameter for further breeding and cultivation programme. Transposable elements (TEs) are a major component of plant genome but still, their evolutionary significance in Pongamia remains unexplored. In view to understand the role of TEs in genome diversity, Pongamia unigenes were screened for the presence of TE cassettes. Our analysis showed the presence of all categories of TE cassettes in unigenes with major contribution of long terminal repeat-retrotransposons towards unigene diversity. Interestingly, the insertion of some TEs was also observed in both organellar genomes. The study of insertion of TEs in coding sequence is of great interest as they may be responsible for protein diversity thereby influencing the phenotype. The present investigation confirms the exaptation phenomenon in pyruvate decarboxylase (PDC) gene where the entire exon sequence was derived from Ty3-gypsy like retrotransposon. The study of PDC protein revealed the translation of gypsy element into protein. Furthermore, the phylogenetic study confirmed the diversity in PDC gene due to insertion of the gypsy element, where the PDC genes with and without gypsy insertion were clustered separately.