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Organophosphate hydrolase (OphB) gene from Pseudomonas sp. was transferred into Arabidopsis plants to observe the bioremediation ability and tolerance level of the transgenic plant to organophosphate pesticides contaminants. Gene transfer was observed by PCR of the transgenic Arabidopsis plants' genomic DNA. Expression of ophB gene and protein levels in the transgenic Arabidopsis plants was observed by western blot analysis. The transgenic plants were resistant and tolerant to chlorpyrifos (an organophosphate pesticide), as evidenced by a toxicity test, where the transgenic plants produced greater shoot and root biomass than that of wild type plants. The fresh weight of transgenic Arabidopsis plants' did not reduced significantly till 400 ppm chlorpyrifos treatment, but fresh weight of wild type Arabidopsis plants' significantly reduced by the application of 100 ppm chlorpyrifos. Moreover, in 600 ppm chlorpyrifos liquid culture, transgenic Arabidopsis plants' produced 1.34 g biomass from 100 seeds, but wild type Arabidopsis plants' produced only 0.24 g biomass from 100 seeds. This study indicates that transgenic Arabidopsis plants having ophB gene increase the tolerance level of organophosphate pesticides.
Assuntos
Arabidopsis , Clorpirifos , Praguicidas , Arabidopsis/genética , Arabidopsis/metabolismo , Arildialquilfosfatase/genética , Arildialquilfosfatase/metabolismo , Clorpirifos/metabolismo , Clorpirifos/toxicidade , Regulação da Expressão Gênica de Plantas , Compostos Organofosforados/metabolismo , Compostos Organofosforados/toxicidade , Praguicidas/metabolismo , Plantas Geneticamente Modificadas/genéticaRESUMO
Full profile of polychlorinated biphenyls (PCBs) in the coastal surface water from Bangladesh were analyzed by GC-MS/MS to explore the status of contamination, spatiotemporal distribution and to trace their potential sources. The total concentrations of dissolved PCBs (∑PCBs, sum of all congeners) varied from 32.17 to 160.7â¯ng/L and 46.45-199.4â¯ng/L in winter and summer, respectively, and the ranges were comparable to or higher than those recorded in the surface water from the coastal areas of India, China, Japan, Italy, Belgium and USA. The difference in the levels of PCBs between the two seasons was not statistically significant (pâ¯>â¯0.05). However, spatial distribution revealed that the areas with recent urbanization and industrialization (Chittagong, Cox's Bazar and Sundarbans) were more contaminated with PCBs than the unindustrialized area (Meghna Estuary). Lightly to moderately chlorinated (2-6 Cl) homologs dominated the PCB profiles. Our analyses (congener profile and homolog composition) elucidated that the past and on-going use of PCB-containing equipment (e.g. capacitors and transformers) as well as the anthropogenic activities such as urban developments, commercial and industrial establishments (e.g. ship breaking and port activities) might be the potential sources of PCB emission in Bangladesh. A set of congeners based on their detection frequencies and abundance were identified and categorized as potential environmental marker PCBs, which can be used for the future selective monitoring studies regarding reasonable limitations on full congener assessment. According to the existing national and international water quality guidelines/standards, PCB concentrations recorded in this study could potentially cause biological damage. Essentially, the findings of this first comprehensive report on the PCB contamination in the surface water in Bangladesh may provide a reference to future studies of these compounds in the Bay of Bengal.
Assuntos
Baías/química , Bifenilos Policlorados/análise , Poluentes Químicos da Água/análise , Bangladesh , Cromatografia Gasosa , Monitoramento Ambiental , Sedimentos Geológicos/química , Estações do Ano , Água do Mar/química , Extração em Fase Sólida , Espectrometria de Massas em TandemRESUMO
This study performs extended x-ray absorption fine structure (EXAFS) measurements for the S-K edge in the temperature range of 10 and 300 K in the transmission mode using a photodiode to detect the transmitted x-rays. It provides the first report of temperature variations in the structural parameters ofα-S. As the temperature increases from 10 to 300 K in the Fourier transform ofkχ(k)the first peak corresponding to the covalent bond of the eight-membered ring becomes slightly low anomalously despite thermal disturbances. However, as in normal materials, the second peak at 300 K decreases to approximately half of that at 10 K, which contains several intra- and inter-ring correlations. All structural parameters of the covalent bond obtained by nonlinear least squares fitting exhibit missing temperature variations. A value of zero for the asymmetric parameter in the EXAFS (C3) implies that the potential of the covalent bond is symmetric, and the constant value of the mean square relative displacement (MSRD) with temperature implies that the potential is extremely high. The Einstein model fitting for the temperature variation in the MSRD yields an Einstein temperature of 942 K and force constant (K) of 405 N m-1. The value ofKis the largest among those of chalcogen elements.
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The UPPS-P Impulsive Behavior Model and the various psychometric instruments developed and validated based on this model are well established in clinical and research settings. However, evidence regarding the psychometric validity, reliability, and equivalence across multiple countries of residence, languages, or gender identities, including gender-diverse individuals, is lacking to date. Using data from the International Sex Survey (N = 82,243), confirmatory factor analyses and measurement invariance analyses were performed on the preestablished five-factor structure of the 20-item short version of the UPPS-P Impulsive Behavior Scale to examine whether (a) psychometric validity and reliability and (b) psychometric equivalence hold across 34 country-of-residence-related, 22 language-related, and three gender-identity-related groups. The results of the present study extend the latter psychometric instrument's well-established relevance to 26 countries, 13 languages, and three gender identities. Most notably, psychometric validity and reliability were evidenced across nine novel translations included in the present study (i.e., Croatian, English, German, Hebrew, Korean, Macedonian, Polish, Portuguese-Portugal, and Spanish-Latin American) and psychometric equivalence was evidenced across all three gender identities included in the present study (i.e., women, men, and gender-diverse individuals).
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This study aimed to determine the prevalence of and factors associated with suicidal ideation among university students in Bangladesh. We collected cross-sectional data from 407 students aged between 18 and 27 years at Jahangirnagar University (Dhaka, Bangladesh), using a convenience sampling technique. Of the total sample, 13.8% reported having suicidal ideation during the past 12 months. In multiple logistic regression analysis, being female, being a fifth-year student, lower socioeconomic status, exposure to traumatic events, family suicide history, and depression were associated with suicidal ideation. The findings emphasize the need for appropriate support services for university students with a focus on mental health wellbeing and suicide prevention.
Assuntos
Estudantes , Ideação Suicida , Adolescente , Adulto , Bangladesh/epidemiologia , Estudos Transversais , Feminino , Humanos , Masculino , Prevalência , Fatores de Risco , Estudantes/psicologia , Universidades , Adulto JovemRESUMO
Antimicrobial resistance (AMR) is a major health crisis globally. Migratory birds could be a potential source for antibiotic resistant (ABR) bacteria. Not much is known about their role in the transmission of ABR in Bangladesh. In this study, a total of 66 freshly dropped fecal materials of migratory birds were analyzed. Bacterial isolation and identification were based on cultural properties, biochemical tests, and polymerase chain reaction (PCR). The disk diffusion method was employed to evaluate antibiogram profiles. By PCR, out of 66 samples, the detection rate of Enterococcus spp. (60.61%; 95% confidence interval: 48.55-71.50%) was found significantly higher than Salmonella spp. (21.21%; 95% CI: 13.08-32.51%) and Vibrio spp. (39.40%; 95% CI: 28.50-51.45%). Enterococcus isolates were frequently found resistant (100-40%) to ampicillin, streptomycin, meropenem, erythromycin, and gentamicin; Salmonella isolates were frequently resistant (72-43%) to chloramphenicol, tetracycline, ampicillin, streptomycin, and erythromycin; and Vibrio spp. isolates were frequently resistant (77-31%) to vancomycin, ampicillin, erythromycin, tetracycline, and streptomycin. In addition, 60% (95% CI: 44.60-73.65%) Enterococcus spp., 85.71% (95% CI: 60.06-97.46%) Salmonella spp., and 76.92% (95% CI: 57.95-88.97%) Vibrio spp. isolates were multi-drug resistant (MDR) in nature. Three isolates (one from each bacterium) were found resistant against six classes of antibiotics. The bivariate analysis revealed strong associations (both positive and negative) between several antibiotic pairs which were resistant to isolated organisms. To the best of our knowledge, this is the first study in detecting MDR Enterococcus spp., Salmonella spp., and Vibrio spp. from migratory birds travelling to Bangladesh. Frequent detection of MDR bacteria from migratory birds travelling to Bangladesh suggests that these birds have the potential to carry and spread ABR bacteria and could implicate potential risks to public health. We recommend that these birds should be kept under an AMR surveillance program to minimize the potential risk of contamination of the environment with ABR as well as to reduce their hazardous impacts on health.
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Online activities such as online gaming and social media use have become very popular and for most individuals, engagement in such activities is highly enjoyable. However, for a small minority of individuals, such behaviors can lead to problematic internet use. There are many screening instruments that assess problematic internet use including the nine-item Internet Disorder Scale-Short-Form (IDS9-SF). The present study translated the IDS9-SF into Bangla and investigated its psychometric properties. The sample comprised 534 adolescents aged 13 to 18 years (61.0% male; mean age 15.7 years (SD = 1.5) from selected schools (secondary and higher secondary) in Dhaka City. The study included confirmatory factor analysis, Cronbach's alpha, and item-total correlation. Results demonstrated that the Bangla IDS9-SF had very good internal validity (Cronbach's alpha = .82), and that IDS score was positively and significantly correlated with scores on depression and anxiety scales. A confirmatory factor analysis showed the model had an excellent fit to the data. Therefore, the Bangla IDS9-SF appears to be a valid and reliable instrument that may be employed in further research on problematic internet use in Bangladesh.
Assuntos
Comportamento Aditivo/psicologia , Transtorno de Adição à Internet/psicologia , Psicometria , Inquéritos e Questionários/normas , Adolescente , Bangladesh , Feminino , Humanos , Masculino , Programas de Rastreamento , Escalas de Graduação Psiquiátrica , Reprodutibilidade dos TestesRESUMO
Heavy metals are known to cause deleterious effects on human health through food chain. Human health risks were evaluated from consumption of heavy metal contaminated fish from Buriganga River in Bangladesh. Whole body of five fish species (Puntius ticto, Puntius sophore, Puntius chola, Labeo rohita and Glossogobius giuris) were analyzed which contained various concentrations of Cd, As, Pb, Cr, Ni, Zn, Se, Cu, Mo, Mn, Sb, Ba, V and Ag. Concentrations of Mn, Zn, Se and Pb in all fish species were above the Food Safety Guideline (FSG) by WHO/FAO. Assessment of noncarcinogenic health hazard by target hazard quotient (THQ) indicated no concern from consumption of these fish except for Mn. However, all metals together may affect human health as revealed by hazard index (HI). The target cancer risk (TR) values suggested carcinogenic risk from Ni and As. Taken together it can be concluded that there is potential human health risk in consuming fish from river Buriganga.