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The remote delivery of optical reference with highly stable oscillation frequency and carrier phase can eliminate the need of digital signal processing for the estimation of these parameters in optical communication. The distribution distance of the optical reference has been limited, however. In this paper, an optical reference distribution over 12,600â km is achieved while maintaining low-noise characteristics, using an ultra-narrow-linewidth laser as a reference source and a fiber Bragg grating filter for noise removal. The distributed optical reference enables 10 GBaud, 5 wavelength-division-multiplexed dual-polarization 64QAM data transmission without using carrier phase estimation, which significantly reduces off-line signal processing time. In the future, this method can enable all coherent optical signals in the network to be synchronized to a common reference ideally, thereby improving overall energy efficiency and cost.
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As COVID-19 continues to spread, infection risk on public transport is concerning. Air exchange rates (ACH) and advection-diffusion of CO2 and particles were determined in a route bus to evaluate the infection risk. ACH increased with bus speed whether windows were open or closed, and ACH were greater when more windows were open. With two open windows, ACH was greater when a front and rear window were open than when two rear windows were open. With both front and rear ventilation fans set to exhaust, ACH was more than double that when both were set to supply. With air conditioning (AC) off, CO2 and particles spread proportionally at the same rate from a source, whereas with the AC on, the spread rate of particles was about half that of CO2 , because particles might be trapped by a prefilter on the AC unit. Infection risk can be reduced by equipping AC unit with an appropriate filter. Calculations with a modified Wells-Riley equation showed that average infection risk was reduced by 92% in the moving bus with windows open comparing to with windows closed. When the bus was moving with windows closed, exhaust fan operation reduced the average risk by 35%.
Assuntos
Poluição do Ar em Ambientes Fechados , COVID-19 , Aerossóis , Poluição do Ar em Ambientes Fechados/análise , Dióxido de Carbono , Humanos , VentilaçãoRESUMO
Brassinosteroid (BR) signalling is known to be coordinated with light signalling in above ground tissue. Many studies focusing on the shade avoidance response in above ground tissue or hypocotyl elongation in darkness have revealed the contribution of the BR signalling pathway to these processes. We previously analysed the expression of DWARF 4 (DWF4), a key BR biosynthesis enzyme, and revealed that light perception in above ground tissues triggered DWF4 accumulation in root tips. To determine the required wavelength of light and photoreceptors responsible for this regulation, we studied DWF4-GUS marker plants grown in several monochromatic light conditions. We revealed that monochromatic blue LED light could induce DWF4 accumulation in primary root tips and root growth as much as white light, whereas monochromatic red LED could not. Consistent with this, a cryptochrome1/2 double mutant showed retarded root growth under white light whereas a phytochromeA/B double mutant did not. Taken together, our data strongly indicated that blue light signalling was important for DWF4 accumulation in root tips and root growth. Furthermore, DWF4 accumulation patterns in primary root tips were not altered by auxin or sugar treatment. Therefore, we hypothesize that blue light signalling from the shoot tissue is different from auxin and sugar signalling.
Assuntos
Proteínas de Arabidopsis/metabolismo , Brassinosteroides/metabolismo , Criptocromos/metabolismo , Sistema Enzimático do Citocromo P-450/metabolismo , Raízes de Plantas/crescimento & desenvolvimento , Arabidopsis , Escuridão , Regulação da Expressão Gênica de Plantas , Transdução de Sinal Luminoso/fisiologia , Raízes de Plantas/metabolismoRESUMO
This paper discusses spatially diverse optical vector network analysis for space division multiplexing (SDM) component and system characterization, which is becoming essential as SDM is widely considered to increase the capacity of optical communication systems. Characterization of a 108-channel photonic lantern spatial multiplexer, coupled to a 36-core 3-mode fiber, is experimentally demonstrated, extracting the full impulse response and complex transfer function matrices as well as insertion loss (IL) and mode-dependent loss (MDL) data. Moreover, the mode-mixing behavior of fiber splices in the few-mode multi-core fiber and their impact on system IL and MDL are analyzed, finding splices to cause significant mode-mixing and to be non-negligible in system capacity analysis.
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We investigate a high-capacity, space-division-multiplexed (SDM) transmission system using self-homodyne detection (SHD) in multi-core fiber (MCF). We first investigate SHD phase noise cancellation with both kHz and MHz range linewidths for both quadrature-phase shift-keyed (QPSK) and 16 quadrature-amplitude modulation (16QAM) signals, finding that phase noise cancellation in SHD enabled transmission with MHz linewidth lasers that resulted in error floors when using intradyne detection. We then demonstrate a high throughput SHD transmission system using low-cost, MHz linewidth distributed feedback lasers. We transmit a CW pilot-tone on a single core of a 10.1 km MCF span with the remaining 18 cores used to transmit 125 wavelength-division multiplexed (WDM) QPSK and polarization-division-multiplexed (PDM)-QPSK signals with 50 GHz channel spacing at 25 GBd. For PDM transmission and assuming a 7% forward-error correction overhead this is equivalent 210 Tb/s transmission with a SE of 33.4 b/s/Hz. High-capacity transmission is achieved despite high inter-core crosstalk, broad transmitter linewidth and narrow channel spacing, showing that combining SHD with MCF enables high throughput, low-cost transmission in next-generation optical networks.
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We report the development of a space division multiplexed (SDM) transmission system consisting of a 19-core fiber and 19-core Erbium-doped fiber amplifier (EDFA). A new 19-core fiber with an improved core arrangement was employed to achieve a low aggregated inter-core crosstalk of -42 dB at 1550 nm over 30 km. The EDFA uses shared free-space optics for pump beam combining and isolation, thus is SDM transparent and has some potential for cost reduction. 19.6 dB to 23.3 dB gain and 6.0 dB to 7.0 dB noise figure were obtained for each SDM channel at 1550 nm. System feasibility for SDM transmission over 1200 km was demonstrated with 100 Gb/s PDM-QPSK signals.
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We investigate the performance of a self-homodyne coherent detection (SHCD) system using a 19 core multi-core fiber (MCF) and 16 wavelength-division-multiplexed channels. We show that SHCD, with the pilot-tone transmitted on a single MCF core and information carrying signals on the remaining cores, is compatible with space-division-multiplexed transmission, potentially relaxing laser linewidth and digital signal processing requirements due to phase noise cancellation. However, inter-core crosstalk can have an impact on performance and core selection.
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Redes de Comunicação de Computadores/instrumentação , Tecnologia de Fibra Óptica/instrumentação , Modelos Teóricos , Telecomunicações/instrumentação , Simulação por Computador , Desenho Assistido por Computador , Desenho de Equipamento , Análise de Falha de EquipamentoRESUMO
In the developmental process of plant cells, cell fate is determined by expression of specific genes. Their expressions are directed by specific transcriptional regulators and positional signals that convey the locations of respective cells along various body axes, including proximaldistal, adaxialabaxial and mediallateral axes. Recently it was reported that some small regulatory RNAs work in a non-cell-autonomous fashion and provide neighboring cells with positional signals. Among such small RNAs, we will review unique biological features of microRNA 165 / 166 widely involved in plant development.
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Arabidopsis/genética , Regulação da Expressão Gênica no Desenvolvimento , Regulação da Expressão Gênica de Plantas , MicroRNAs/fisiologia , Padronização Corporal , Genes de Plantas , MicroRNAs/genética , Folhas de Planta/genética , Proteínas de Plantas/genética , Raízes de Plantas/metabolismo , Fatores de Transcrição/metabolismoRESUMO
Leaf veins have a complex network pattern. Formation of this vein pattern has been widely studied as a model of tissue pattern formation in plants. To understand the molecular mechanism governing the vascular patterning process, we isolated the rice mutant, commissural vein excessive1 (coe1). The coe1 mutants had short commissural vein (CV) intervals and produced clustered CVs. Application of 1-N-naphthylphthalamic acid and brefeldin A decreased CV intervals, and application of 1-naphthaleneacetic acid increased CV intervals in wild-type rice; however, coe1 mutants were insensitive to these chemicals. COE1 encodes a leucine-rich repeat receptor-like kinase, whose amino acid sequence is similar to that of brassinosteroid-insensitive 1-associated receptor kinase 1 (BAK1), and which is localized at the plasma membrane. Because of the sequence similarity of COE1 to BAK1, we also examined the involvement of brassinosteroids in CV formation. Brassinolide, an active brassinosteroid, decreased the CV intervals of wild-type rice, and brassinazole, an inhibitor of brassinosteroid biosynthesis, increased the CV intervals of wild-type rice, but coe1 mutants showed insensitivity to these chemicals. These results suggest that auxin and brassinosteroids regulate CV intervals in opposite directions, and COE1 may regulate CV intervals downstream of auxin and brassinosteroid signals.
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Oryza/crescimento & desenvolvimento , Proteínas de Plantas/fisiologia , Brassinosteroides/metabolismo , Brefeldina A/farmacologia , Expressão Gênica , Genes de Plantas , Gravitropismo , Ácidos Indolacéticos/metabolismo , Mutação , Ácidos Naftalenoacéticos/farmacologia , Oryza/genética , Proteínas de Plantas/genética , Transdução de Sinais , Esteroides Heterocíclicos/metabolismoRESUMO
To identify potential countermeasures for coronavirus disease (COVID-19), we determined the air exchange rates in stationary and moving train cars under various conditions in July, August, and December 2020 in Japan. When the doors were closed, the air exchange rates in both stationary and moving trains increased with increasing area of window-opening (0.23-0.78/h at 0 m2, windows closed to 2.1-10/h at 2.86 m2, fully open). The air exchange rates were one order of magnitude higher when doors were open than when closed. With doors closed, the air exchange rates were higher when the centralized air conditioning (AC) and crossflow fan systems (fan) were on than when off. The air exchange rates in moving trains increased as train speed increased, from 10/h at 20 km/h to 42/h at 57 km/h. Air exchange rates did not differ significantly between empty cars and those filled with 230 mannequins representing commuters. The air exchange rates were lower during aboveground operation than during underground. Assuming that 30-300 passengers travel in a train car for 7-60 min and that the community infection rate is 0.0050-0.30%, we estimated that commuters' infection risk on trains was reduced by 91-94% when all 12 windows were opened (to a height of 10 cm) and the AC/fan was on compared with that when windows were closed and the AC/fan was off.
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Microbiologia do Ar , Poluição do Ar em Ambientes Fechados , COVID-19 , Ferrovias , Ventilação , Ar Condicionado , COVID-19/transmissão , Humanos , SARS-CoV-2RESUMO
The fastest known operation of all-optical flip-flop memory was experimentally demonstrated using a 980-nm polarization bistable vertical-cavity surface-emitting laser (VCSEL). Operating conditions of the input signal power and the frequency detuning to achieve the fast optical memory operation were characterized experimentally. At the optimum condition, 1-bit data signals were arbitrarily sampled and memorized from a 2(6)-1 pseudorandom bit sequence return-to-zero signal at 20 Gb/s by using AND gate and memory functionalities obtained from the polarization bistability. In addition, 1-bit memory operation was achieved for a 6-bit non-return-to-zero signal at 40 Gb/s. Both memory operations required 250-microW data signal power and had optical gain. The high potential of all-optical flip-flop memories based on polarization bistable VCSELs for use in ultrafast all-optical future networks was demonstrated.
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Based on nine up-to-date types of semiconductor-optical-amplifier (SOA) samples, we devised a power-consumption model of SOA-based all-optical gates as a tool to develop faster and more efficient OTDM systems for bitrates from 10 to 160 Gb/s and those over 160 Gb/s. The conventional effect of a continuous wave (cw) holding beam was included in the model. Furthermore, in this work we defined three step-wise quantum conversion efficiencies eta(1), eta(2), and eta(3) from current-injected carriers through photons. The dependence of each of the three efficiencies on the SOA-structure was studied. The total efficiency eta(T) observed for the nine SOAs ranged widely from 0.07 to 0.46. The validity of the power-consumption model was verified by systematically measuring the effective carrier recovery rate. According to our model, the power consumption of the best existing SOA-based gate for 160-Gb/s signals is 750 mW, and this increases at a rate approximately proportional to (bitrate)(2), and decreases proportionally to (1/etaT)(2).
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Many attempts have been made to characterize the activities of brassinosteroids (BRs), which are important plant hormones. The crosstalk between light perception and the BR signalling pathway has been extensively studied regarding its effects on photomorphogenesis, especially in elongating etiolated hypocotyls. In contrast, how and where the light induces BR biosynthesis remain uncharacterized. DWF4 is one of the main enzymes involved in the BR biosynthesis pathway in Arabidopsis thaliana. We established DWF4-GUS A. thaliana lines in a homozygous dwf4-102 genetic background, but functionally complemented with a genomic DWF4 sequence fused in-frame with a ß-glucuronidase (GUS) marker gene. The DWF4-GUS plants enabled the visualization of the accumulation of DWF4 under different conditions. We investigated the effects of aboveground light on root and hypocotyl growth. We observed that root length increased when shoots were maintained under light irrespective of whether roots were exposed to light. We also determined that light perception in aerial tissues enhanced DWF4 accumulation in the root tips. Overall, our data indicate that BR biosynthesis is promoted in the root tip regions by an unknown mechanism in distantly located shoot tissues exposed to light, leading to increased root growth.
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Sistema Enzimático do Citocromo P-450/metabolismo , Luz , Componentes Aéreos da Planta/fisiologia , Raízes de Plantas/fisiologia , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Sistema Enzimático do Citocromo P-450/genética , Regulação da Expressão Gênica de Plantas , Fenótipo , Fotossíntese , Proteínas Recombinantes de FusãoRESUMO
In frequency ranges above 200-300 GHz, the second slowest relaxation in the optical response (such as carrier-cooling relaxation having a time constant of 1-2 ps) of a semiconductor optical amplifier inside the conventional delayed-interference signal-wavelength converter (DISC) scheme is thought to start the distortion of all-optically gated waveforms. In this work, we design a digital optical-spectrum-synthesizer block that is part of the expanded DISC scheme. Our numerically calculated spectra, waveforms, and eye diagrams with assumed pseudorandom digital data pulses indicate that this synthesizer significantly removes strong distortion from the gated waveforms. A signal-to-noise ratio of 20 dB was obtained from our random-data eye diagram, providing proof of effectiveness in principle.
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The impact of ultra-fast carrier dynamics in semiconductor optical amplifiers (SOAs) on switches based on cross-gain and cross-phase modulation is analyzed theoretically and experimentally. We find that ultra-fast effects lead to additional spectral broadening, which improves the optical signal-to-noise ratio for switches based on an SOA and an optical filter. For such switches, the influence of ultra-fast effects on the so-called nonlinear patterning effect is analyzed for three filter configurations: the asymmetric Mach-Zehnder interferometer (AMZI), a band-pass filter (BPF), and a cascade of an AMZI and a BPF. We conclude that fast carrier dynamics dramatically reduces nonlinear patterning and that the successful high-speed (>100 Gb/s) demonstrations in the literature rely on these effects.
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We identify a fundamental difference between the ASE noise filtering properties of different all-optical SOA-based switch configurations, and divide the switches into two classes. An in-band ASE suppression ratio quantifying the difference is derived theoretically and the impact of the ASE filtering on the optical spectrum is verified experimentally using a hybrid DISC setup. ASE power suppression of around 3 dB over the total signal bandwidth is demonstrated.
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Vascular development is a central theme in plant science. However, little is known about the mechanism of vascular development in monocotyledons (compared with dicotyledons). Therefore, we investigated sequential processes of differentiation into various different vascular cells by carrying out detailed observations using serial sections of the bases of developing leaves of rice and maize. The developmental process of the longitudinal vascular bundles was divided into six stages in rice and five stages in maize. The initiation of differentiation into procambial progenitor cells forming the commissural vein arose in a circular layer cell that was adjacent to both a metaxylem vessel and one or a few phloem cells in stage V longitudinal vascular bundles. In most cases the differentiation of ground meristem cells into procambial progenitor cells extended in one direction, toward the next longitudinal vascular bundle, and subsequent periclinal divisions and further differentiation produced a vessel element, two companion cells and a sieve element to form a commissural vein. These results suggest the presence of an intercellular signal(s) that induces differentiation of the circular layer cell and the ground meristem cells into procambial progenitor cells, forming a commissural vein sequentially.
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Diferenciação Celular , Oryza/citologia , Folhas de Planta/citologia , Zea mays/citologiaRESUMO
Structural modification of imiquimod (1), which is known as an interferon-alpha (IFN-alpha) inducer, for the aim of finding a novel and small-molecule tumor necrosis factor-alpha (TNF-alpha) suppressor and structure-activity relationship (SAR) are described. Structural modification of a imiquimod analogue, 4-amino-1-[2-(1-benzyl-4-piperidyl)ethyl-1H-imidazo[4,5-c]quinoline (2), which had moderate TNF-alpha suppressing activity without IFN-alpha inducing activity, led to a finding of 4-chloro-2-phenyl-1-[2-(4-piperidyl)ethyl]-1H-imidazo[4,5-c]quinoline (10) with potent TNF-alpha suppressing activity. The relation between conformational direction of 2-(4-piperidyl)ethyl group at position 1 and TNF-alpha suppressing activity is also demonstrated by NMR.