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INTRODUCTION: The clinical learning environment is the context of overlap between the educational system and the working environment. Here students apply the knowledge learned during theoretical teachings, acquire practical, relational and caring skills. It is fundamental for nursing training and there are several rating scales aimed at evaluating it: the presence and quality of the studies that investigate their performances are heterogeneous, so it is difficult to identify the best tool. The CLEI-19 scale is the most concise instrument and its performance has not been tested in the Italian context. OBJECTIVES: The study aims to evaluate the psychometric properties of the instrument in terms of factor structure and reliability. METHODS: A multicenter cross-sectional observational study was conducted in Italy, at the University of Milan-Bicocca. Two tools were used: a context data collection questionnaire and the Italian CLEI-19 scale. The data were analyzed through descriptive statistics, the scale's factorial structure was tested by Exploratory Structural Equation Modeling ESEM. The reliability of the scale was evaluated by Alpha and Omega coefficients. RESULTS: The sample consists of 1095 statistical units. The factor analysis shows the presence of two latent factors that have been called Internship Assistant and Internship Environment. They correlate significantly and the fit indices confirm the solidity of the two-factor model. Internal coherence was adequate or more than adequate for single factors and overall tool. CONCLUSIONS: The CLEI-19 scale is a reliable tool, which comprehensively investigates the two key components of the clinical learning environment: the Internship Assistant and the Environment. The employment simplifies the process of evaluating the internship environment, offers the possibility of improving its quality, facilitates the comparison between realities and enhances the perception of students.
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Educação em Enfermagem , Estudantes de Enfermagem , Humanos , Psicometria , Estudos Transversais , Reprodutibilidade dos Testes , Inquéritos e QuestionáriosRESUMO
Recent developments in 3D graphic technologies enable the affordable and precise reconstruction of body scanned models that can be applied in a variety of verticals, such as fashion, fitness and wellness, and healthcare. The accuracy of body measurements is a crucial element for the successful application of avatars in the following use cases: Avatars that go beyond visual representation and offer intrinsic and precise anthropometric data defined as a smart body are discussed in this paper. In particular, this paper presents the Gate technology, an innovative, autonomous, sustainable body scanner, coupled with an automatic production pipeline and the concept of avatars as smart bodies. We present an accuracy study of scanning technology for scanning inanimate objects, as well as body parts versus the ground, by using an established accuracy scanning system. The results appear to be promising and confirm the hypothesis of applying the technology to the use cases discussed as well as broadening the research to other studies and future applications.
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BACKGROUND: The production of microbial lipids has attracted considerable interest during the past decade since they can be successfully used to produce biodiesel by catalyzed transesterification with short chain alcohols. Certain yeast species, including several psychrophilic isolates, are oleaginous and accumulate lipids from 20 to 70% of biomass under appropriate cultivation conditions. Among them, Rhodotorula glacialis is a psychrophilic basidiomycetous species capable to accumulate intracellular lipids. RESULTS: Rhodotorula glacialis DBVPG 4785 is an oleaginous psychrophilic yeast isolated from a glacial environment. Despite its origin, the strain abundantly grew and accumulated lipids between -3 to 20°C. The temperature did not influence the yield coefficients of both biomass and lipids production, but had positive effect on the growth rate and thus on volumetric productivity of lipid. In glucose-based media, cellular multiplication occurred first, while the lipogenic phase followed whenever the culture was limited by a nutrient other than glucose. The extent of the carbon excess had positive effects on triacylglycerols production, that was maximum with 120 g L-1 glucose, in terms of lipid concentration (19 g L-1), lipid/biomass (68%) and lipid/glucose yields (16%). Both glucose concentration and growth temperature influenced the composition of fatty acids, whose unsaturation degree decreased when the temperature or glucose excess increased. CONCLUSIONS: This study is the first proposed biotechnological application for Rhodotorula glacialis species, whose oleaginous biomass accumulates high amounts of lipids within a wide range of temperatures through appropriate cultivation C:N ratio. Although R. glacialis DBVPG 4785 is a cold adapted yeast, lipid production occurs over a broad range of temperatures and it can be considered an interesting microorganism for the production of single cell oils.
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Lipídeos/biossíntese , Rhodotorula/crescimento & desenvolvimento , Biocombustíveis , Biomassa , Temperatura Baixa , Ácidos Graxos/biossíntese , Glucose/químicaRESUMO
One of the many different skills that nursing students have to achieve is how to dispense care by applying nursing concepts. The authors propose and instrument for evaluating and monitoring various aspects of learning in nursing students and their ability to put them into practice by considering 31 indicators, taking into account the degree of difficulty (D) and discrimination (d) involved in each one. All but 2 of the indicators were found to be reliable for assessing learning in nursing students . An overall statistical analysis and an analysis of the scores obtained enabled the tutors to establish an evaluation score for the nursing plans elaborated by the students.
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Educação em Enfermagem , Aprendizagem , Processo de EnfermagemRESUMO
Obligate psychrophilic, facultative psychrophilic, and mesophilic yeasts were cultured in a carbon-rich medium at different temperatures to investigate whether growth parameters, lipid accumulation, and fatty acid (FA) composition were adaptive and/or acclimatory responses. Acclimation of facultative psychrophiles and mesophiles to a lower temperature decreased their specific growth rate, but did not affect their biomass yield (Y(X/S)). Obligate and facultative psychrophiles exhibited the highest Y(X/S). Acclimation to lower temperature decreased the lipid yield (Y(L/X)) in mesophilic yeasts, but did not affect Y(L/X) in facultative psychrophilic ones. Similar Y(L/X) were found in both groups of psychrophiles, suggesting that lipid accumulation is not a distinctive characteristic of adaptation to permanently cold environments. The unsaturation of FAs was one major adaptive feature of the yeasts colonizing permanently cold ecosystems. Remarkable amounts of alpha-linolenic acid were found in obligate psychrophiles at the expense of linoleic acid, whereas it was scarce or absent in all the other strains. Increased unsaturation of FAs was also a general acclimatory response of facultative psychrophiles to a lower temperature. These results improve the knowledge of the responses enabling psychrophilic yeasts to cope with the cold and may be of support for potential biotechnological exploitation of these strains.
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Ácidos Graxos/biossíntese , Metabolismo dos Lipídeos , Leveduras/crescimento & desenvolvimento , Leveduras/metabolismo , Aclimatação , Temperatura Baixa , Ácido alfa-Linolênico/biossínteseRESUMO
Bladder outlet obstruction can negatively affect renal function in the kidney transplant population. Functional obstruction represents a clinical challenge as conventional treatments are either ineffective or not recommended. Sacral neuromodulation was successfully used in functional voiding dysfunction in a kidney transplant patient. This is the first case to be reported in the literature.