Development and efficacy assessment of polyherbal phytosomal gel for accelerated wound healing.

Curcuma longa L. and Plumbago zeylanica L. are renowned for their antioxidant, anti-inflammatory, and wound-healing properties, primarily attributed to their polyphenolic compounds. However, the limited water solubility of these compounds poses challenges to their effective utilization. Encapsulation within phytosomes offers a solution by enhancing bioavailability and permeability. This study aimed to formulate a phytosome-based polyherbal gel incorporating methanolic extracts of P. zeylanica and C. longa to explore its potential in wound healing. Methanolic extracts of P. zeylanica roots and C. longa rhizomes were encapsulated in phytosomes using the lipid film hydration technique. Various phytosome formulations were developed and characterized for encapsulation efficiency, particle size, polydispersity index and zeta potential. The optimized phytosomal dispersion (F7) was integrated into a carbopol-based hydrogel matrix. In vitro release studies demonstrated prolonged release compared to conventional forms. Stability testing confirmed the robustness of the phytosomal gel at 4 °C/60 ± 5% RH. Wound healing activity was assessed using an excision wound model. The phytosomal gel exhibited enhanced wound contraction and reduced epithelization time compared to conventional gel and control groups, signifying its potent wound-healing effect. In conclusion, the polyherbal phytosomal gel, incorporating P. zeylanica and C. longa, holds promise in promoting wound healing, presenting a novel and effective approach in the realm of topical formulations for wound care.

Development and Validation of HPLC Method for Quantification of Plumbagin in Plumbago Zeylanica L. Roots.

BACKGROUND: An RP-HPLC (Reverse Phase-High-performance liquid chromatography) method for the quantitative estimation and validation of the plumbagin in the methanolic fraction of Plumbago zeylanica L. was developed. METHOD: For achieving good separation, the RP-HPLC method was carried out with reverse phase C18 column, using methanol and water as mobile phase in the ratio of 65:35 (v/v), at the flow rate of 1 mL/min. The detection wavelength was set at 265 nm. RESULTS: The retention time of plumbagin was found at 7.5±0.2 min. The coefficient of determination of plumbagin was found to be (r2) 0.9985 and equation Y=23148x+4327. The LOD and LOQ were found to be 34.06 and 113 ng/mL, respectively. CONCLUSION: The developed method was accurate, specific, precise, and reproducible. This RP-HPLC may be useful for quantitative estimation of the chemical constituents present in the plant extract as well as the quality assessment of the herbal product.

A Recent Overview on Dermatological Applications of Liposomes.

BACKGROUND: In dermatology, the topical application of liposomes has proven to be of therapeutic value. Many drugs encapsulated into liposomes have shown enhanced skin penetration. METHODS: Liposomes are lipid based spherical vesicles. In structure and composition, they resemble cell membrane. Because of their amphipathic nature, they can be used as carriers for hydrophilic as well as lipophilic therapeutic agents. These liposomal systems can easily get integrated with the skin lipids and maintain the desired hydration conditions to improve drug penetration and localization in the skin layers. RESULTS: Considering the need for topical delivery and the promising potential of liposomes, an attempt has been made to explore the recent advances of liposome-based formulations in dermatological applications. CONCLUSION: This patent summarizes the recent findings of liposome-based formulations for dermal delivery of drugs.