Improved cross-talk suppression for digitally enhanced interferometry using Golay complementary pairs.

We demonstrate digitally enhanced interferometry with better than 100 dB mean cross-talk suppression with Golay complementary pairs using a combination of numerical simulations and experiments. These results exceed previously reported cross-talk suppression using conventional maximal length sequences by more than 48 dB.

Algebraic cancellation of inter-channel cross talk in multiplexed heterodyne interferometry.

We demonstrate the algebraic cancellation of residual phase cross talk in digitally enhanced heterodyne interferometry (DEHeI), a code division multiplexing technique for interferometric sensing. By using linear combinations of parallel decoding operations at multiple delays, we synthesize a zero correlation for spurious signals and remove phase cross talk: a method we call offset decoding. We experimentally demonstrate 70 dB of signal isolation and over 40 dB greater isolation than the equivalent standard implementation of DEHeI.

Cyanotoxins within and Outside of Microcystis aeruginosa Cause Adverse Effects in Rainbow Trout (Oncorhynchus mykiss).

The global expansion of toxic Microcystis blooms, and production of cyanotoxins including microcystins, are an increasing risk to freshwater fish. Differentiating intracellular and extracellular microcystin toxicity pathways (i.e., within and outside of cyanobacterial cells) in fish is necessary to assess the severity of risks to populations that encounter harmful algal blooms in pre-to-postsenescent stages. To address this, adult and juvenile Rainbow Trout (Oncorhynchus mykiss) were, respectively, exposed for 96 h to intracellular and extracellular microcystins (0, 20, and 100 µg L-1) produced by Microcystis aeruginosa. Fish were dissected at 24 h intervals for histopathology, targeted microcystin quantification, and nontargeted proteomics. Rainbow Trout accumulated intracellular and extracellular microcystins in all tissues within 24 h, with greater accumulation in the extracellular state. Proteomics revealed intracellular and extracellular microcystins caused sublethal toxicity by significantly dysregulating proteins linked to the cytoskeletal structure, stress responses, and DNA repair in all tissues. Pyruvate metabolism in livers, anion binding in kidneys, and myopathy in muscles were also significantly impacted. Histopathology corroborated these findings with evidence of necrosis, apoptosis, and hemorrhage at similar severity in both microcystin treatments. We demonstrate that sublethal concentrations of intracellular and extracellular microcystins cause adverse effects in Rainbow Trout after short-term exposure.

Crosstalk reduction for multi-channel optical phase metrology.

Digitally enhanced heterodyne interferometry (DEHI) combines the sub-wavelength displacement measurements of conventional laser interferometry with the multiplexing capabilities of spread-spectrum modulation techniques to discriminate between multiple electric fields at a single photodetector. Technologies that benefit from DEHI include optical phased arrays, which require the simultaneous phase measurement of a large number of electric fields. A consequence of measuring the phase of multiple electric fields is the introduction of crosstalk, which can degrade measurement precision. This work analytically and experimentally investigates the crosstalk when using DEHI to measure the phase of an arbitrarily large number of electric fields at a single photodetector. Also considered is the practical limit the dynamic range of the photodetector and shot noise imposes on the number of electric fields that can be discriminated. We describe how to minimize crosstalk by design. Experimental results demonstrate up to 55 dB suppression of crosstalk between two electric fields with a phase measurement bandwidth of 20 kHz and 1-10 pm/Hz displacement sensitivity for audio frequencies. Additionally, we demonstrate scaling of crosstalk proportional to the square-root of the number of electric fields when using an M-sequence modulation. Based on this analysis, we estimate that digitally enhanced heterodyne interferometry should be capable of measuring the phase of several hundreds of electric fields at a single photodetector while maintaining the same measurement bandwidth.

Fast beam steering with an optical phased array.

Optical phased arrays (OPAs) are devices that use the coherence of light to control the interference pattern in the far field, which enables them to steer a laser beam with no moving parts. As such, OPAs have potential applications in laser communications, target acquisition and tracking, metrology, and directed energy. In this Letter, we present a control architecture for an actively phase-locked OPA, capable of steering a laser beam at speeds limited by the actuation bandwidth of electro-optic modulators. The system achieved an output phase stability of λ/770 and steering speeds up to 1 MHz. The digital control architecture can be extended to GHz steering speeds, is readily scalable to hundreds of emitters, and is compatible with high-power arrays.

Forest management influences the effects of streamside wet areas on stream ecosystems.

Riparian zones contain areas of strong hydrological connectivity between land and stream, referred to as variable source areas (VSAs), and are considered biogeochemical control points. However, little is known about whether VSAs influence stream communities and whether this connectivity is affected by forest management. To address this, we used multiple biotic and abiotic indicators to (1) examine the influence of VSAs on riparian vegetation and stream ecosystems by comparing VSA and non-VSA reaches and (2) explore how forest management may affect the influence of VSAs on stream ecosystems. We detected some significant differences between VSA and non-VSA reaches in the riparian vegetation (greater understory and lower tree density) and stream ecosystem indicators (greater dissolved organic matter aromaticity, microbial biomass, peroxidase activity and collector-gatherer density, and lower dissolved organic carbon concentrations, algal biomass, and predatory macroinvertebrate density), which suggests that VSAs may create a more heterotrophic ecosystem locally. However, we show some evidence that forest management activities (specifically, road density) can alter the influence of VSAs and eliminate the differences observed at lower forest management intensities, and that the most hydrologically connected areas seem more sensitive to disturbance. Therefore, we suggest that the heterogeneity in hydrological connectivity along riparian zones should be considered when planning forest harvesting operations and road building (e.g., wider riparian buffers around VSAs).

Assessing the toxicity of cell-bound microcystins on freshwater pelagic and benthic invertebrates.

Cyanobacterial harmful algal blooms dominated by Microcystis frequently produce microcystins, a family of toxins capable of inflicting harm to pelagic and benthic freshwater invertebrates. Research on the effect of microcystins on invertebrates is inconclusive; from one perspective, studies suggest invertebrates can coexist in toxic blooms; however, studies have also measured negative food-associated effects from microcystins. To test the latter perspective, we examined the reproduction, growth, and survival of laboratory-cultured Ceriodaphnia dubia, Daphnia magna, and Hexagenia spp. exposed to cell-bound microcystins through a series of life-cycle bioassays. Test organisms were exposed to a concentration gradient ranging from 0.5 µg L-1 to 300 µg L-1 microcystins, which corresponds to values typically found in freshwaters during bloom season. Lethal concentrations in C. dubia (LC50 = 5.53 µg L-1) and D. magna (LC50 = 85.72 µg L-1) exposed to microcystins were among the lowest recorded to date, and reproductive effects were observed at concentrations as low as 2.5 µg L-1. Length of D. magna was significantly impacted in microcystin treatments great than 2.5 µg L-1. No lethality or growth impairments were observed in Hexagenia. This information will improve our understanding of the risks posed by microcystins to food webs in freshwaters.

Increased reliance of stream macroinvertebrates on terrestrial food sources linked to forest management intensity.

Our understanding of how forest management practices affect the relative importance of autochthonous vs. allochthonous resource use by headwater stream food webs is relatively poor. To address this, we used stable isotope (C, N, and H) analyses of food sources and macroinvertebrates from 15 streams in New Brunswick (Canada) and assessed how different catchment conditions arising from the gradient in forest management intensity affect the contribution of autochthonous resources to these food webs. Aquatic primary production contributed substantially to the biomass of invertebrates in these headwater streams, especially for scrapers and collector-gatherers (25-75%). However, the contribution of algae to food webs decreased as forest management intensity (road density and associated sediments, water cations/carbon, and dissolved organic matter humification) increased, and as canopy openness decreased. This trend was probably due to an increase in the delivery of organic and inorganic terrestrial materials (dissolved and in suspension) in areas of greater harvesting intensity and road density, which resulted in more heterotrophic biofilms. Overall, results suggest that, despite the presence of riparian buffers, forest management can affect stream food web structure via changes in energy flows, and that increased protection should be directed at minimizing ground disturbance in areas with direct hydrological connection to streams and at reducing dissolved and particulate matter inputs from roads and stream crossings in catchments with high degrees of management activity.

Pervasive interactions between methyl torsion and low frequency vibrations in S0 and S1p-fluorotoluene.

We report two dimensional laser induced fluorescence spectral images exploring the lower torsion-vibration manifolds in S0 (E < 560 cm-1) and S1 (E < 420 cm-1) p-fluorotoluene. Analysis of the images reveals strong torsion-vibration interactions and provides an extensive set of torsion-vibration state energies in both electronic states (estimated uncertainty ±0.2 cm-1), which are fit to determine key constants including barrier heights, torsional constants, and torsion-vibration interaction constants. The dominant interactions in both electronic states are between methyl torsion (internal rotation) and the lowest frequency out-of-plane modes, D20 and D19, both of which involve a methyl wagging motion. This is the second aromatic (following toluene) for which a significant interaction between torsion and methyl out-of-plane wagging vibrations has been quantified. Given the generic nature of this motion in substituted toluenes and similar molecules, this mechanism for torsion-vibration coupling may be common in these types of molecules. The inclusion of torsion-vibration coupling affects key molecular constants such as barrier heights and torsional (and rotational) constants, and the possibility of such an interaction should thus be considered in spectral analyses when determining parameters in these types of molecules. p-Fluorotoluene is the first molecule in which the role of methyl torsion in promoting intramolecular vibrational energy redistribution (IVR) was established and the observed torsion-vibration coupling provides one conduit for the state mixing that is a precursor to IVR, as originally proposed by Moss et al. [J. Chem. Phys. 86, 51 (1987)].

Relative chronic sensitivity of neonicotinoid insecticides to Ceriodaphnia dubia and Daphnia magna.

Neonicotinoid insecticides are a group of plant protectants frequently detected in surface waters at low concentrations. Aquatic invertebrates therefore have the potential to be exposed chronically to low concentrations of neonicotinoids. The cladocerans Daphnia magna and Ceriodaphnia dubia are among the most commonly used invertebrate test species in aquatic toxicology. Both species are known to be acutely insensitive to neonicotinoids, and while chronic toxicity has been characterized for D. magna, little research has been conducted with C. dubia. In the present study we conducted 7-d static-renewal life cycle tests for 6 neonicotinoids (acetamiprid, clothianidin, dinotefuran, imidacloprid, thiacloprid, and thiamethoxam) with C. dubia, and a 21-d test with imidacloprid with D. magna. 7-d LC50s for C. dubia ranged from 8.42 mg L-1 for imidacloprid to > 100 mg L-1 for clothianidin; 7-d reproduction EC50s were 2.98 for thiacloprid, to > 67 mg L-1 for dinotefuran. D. magna were less sensitive than C. dubia to imidacloprid, by 4-fold for lethality and 1.5-fold for reproduction; however, acute-to-chronic ratios (ACRs) were similar. ACRs, based on 48-h acute LC50s and 7- or 21-d chronic reproduction EC10s, ranged from 5.4 for acetamiprid to 53.0 for imidacloprid (mean 36.6, CV = 51%). Chronic toxicity values for both species were orders of magnitude greater than concentrations reported in the environment, and thus hazard to these cladocerans is negligible.

Investigation of clearance rate as an endpoint in toxicity testing with freshwater mussels (Unionidae).

The implementation of ecologically relevant sub-lethal endpoints in toxicity testing with freshwater mussels can provide valuable information during risk assessment, especially since these organisms are often exposed to low levels of contaminants. This study examined how to optimize quantifying the filtering capacity or clearance rate (CR) of mussels after exposure to a reference toxicant, sodium chloride (NaCl). CR was defined as the number of algal cells an individual mussel can remove from the overlying water by filtration over time and was determined using spectrophotometric absorbance and direct microscopic examination. Optimization included consideration of the following factors: concentration of algae mixture at test initiation, duration of CR assay, and statistical power. Experimental vessels contained either juvenile (ten, ~ 4 months old) or adult (one, ~ 2.5 years old) Lampsilis siliquoidea. To detect a 10% change in filtering capacity, the optimized adult CR assay was run for 48 h with 2.7 × 107 cells/mL of algae added at test initiation and a minimum of 6 replicates per treatment. The optimized juvenile mussel CR assay was run for 48 h with 1.77 × 107 cells/mL of algae added at test initiation; however, 13 replicates would be required to detect a 10% change to satisfy each method. To reduce the number of juvenile mussels used in testing, a minimum of 4 replicates per treatment was recommended to detect a 25% change in CR. After exposure to a reference toxicant (NaCl), EC50s from the optimized CR assay were compared to two other mussel toxicity endpoints: survival and burial (ability of mussels to bury in clean sand). CR by direct microscopic examination was slightly more sensitive than survival and burial in juveniles and only slightly more sensitive than survival in adults. No significant differences (p > 0.05) were detected between the EC/LC50 values determined from CR and the less labour-intensive survival and burial endpoints. The present study suggests the CR for juvenile and adult L. siliquoidea remained largely unaffected in mussels that survived a 7-day NaCl exposure.

High power compatible internally sensed optical phased array.

The technical embodiment of the Huygens-Fresnel principle, an optical phased array (OPA) is an arrangement of optical emitters with relative phases controlled to create a desired beam profile after propagation. One important application of an OPA is coherent beam combining (CBC), which can be used to create beams of higher power than is possible with a single laser source, especially for narrow linewidth sources. Here we present an all-fiber architecture that stabilizes the relative output phase by inferring the relative path length differences between lasers using the small fraction of light that is back-reflected into the fiber at the OPA's glass-air interface, without the need for any external sampling optics. This architecture is compatible with high power continuous wave laser sources (e.g., fiber amplifiers) up to 100 W per channel. The high-power compatible internally sensed OPA was implemented experimentally using commercial 15 W fiber amplifiers, demonstrating an output RMS phase stability of λ/194, and the ability to steer the beam at up to 10 kHz.

Benthic algae compensate for phytoplankton losses in large aquatic ecosystems.

Anthropogenic activities can induce major trophic shifts in aquatic systems, yet we have an incomplete understanding of the implication of such shifts on ecosystem function and on primary production (PP) in particular. In recent decades, phytoplankton biomass and production in the Laurentian Great Lakes have declined in response to reduced nutrient concentrations and invasive mussels. However, the increases in water clarity associated with declines in phytoplankton may have positive effects on benthic PP at the ecosystem scale. Have these lakes experienced oligotrophication (a reduction of algal production), or simply a shift in autotrophic structure with no net decline in PP? Benthic contributions to ecosystem PP are rarely measured in large aquatic systems, but our calculations based on productivity rates from the Great Lakes indicate that a significant proportion (up to one half, in Lake Huron) of their whole-lake production may be benthic. The large declines (5-45%) in phytoplankton production in the Great Lakes from the 1970s to 2000s may be substantially compensated by benthic PP, which increased by up to 190%. Thus, the autotrophic productive capacity of large aquatic ecosystems may be relatively resilient to shifts in trophic status, due to a redirection of production to the near-shore benthic zone, and large lakes may exhibit shifts in autotrophic structure analogous to the regime shifts seen in shallow lakes.

Modeling uptake of selected pharmaceuticals and personal care products into food crops from biosolids-amended soil.

Biosolids contain a variety of pharmaceuticals and personal care products (PPCPs). Studies have observed the uptake of PPCPs into plants grown in biosolids-amended soils. This study examined the ability of Dynamic Plant Uptake (DPU) model and Biosolids-amended Soil Level IV (BASL4) model to predict the concentration of eight PPCPs in the tissue of plants grown in biosolids-amended soil under a number of exposure scenarios. Concentrations in edible tissue predicted by the models were compared to concentrations reported in the literature by calculating estimated human daily intake values for both sets of data and comparing them to an acceptable daily intake value. The equilibrium partitioning (EqP) portion of BASL4 overpredicted the concentrations of triclosan, triclocarban, and miconazole in root and shoot tissue by two to three orders of magnitude, while the dynamic carrot root (DCR) portion overpredicted by a single order of magnitude. DPU predicted concentrations of triclosan, triclocarban, miconazole, carbamazepine, and diphenhydramine in plant tissues that were within an order of magnitude of concentrations reported in the literature. The study also found that more empirical data are needed on the uptake of cimetidine, fluoxetine, and gemfibrozil, and other ionizable PPCPs, to confirm the utility of both models. All hazard quotient values calculated from literature data were below 1, with 95.7% of hazard quotient values being below 0.1, indicating that consumption of the chosen PPCPs in plant tissue poses de minimus risk to human health.

Characterization of Springtail (Arrhopalites caecus) for Use in Soil Ecotoxicity Testing.

Springtails (subclass: Collembola) represent one of the most extensively studied invertebrate groups in soil ecotoxicology. This is because of their ease of laboratory culture, significant ecological role, and sensitivity to environmental contaminants. Folsomia candida (family: Isotomidae) is a globally widespread parthenogenetic species that is prevalent in laboratory toxicity testing with springtails. Conversely, Arrhopalites caecus (family: Arrhopalitidae), a parthenogenic globular springtail species, remains untested in soil ecotoxicology. This species is found in diverse habitats, including cave systems and forest leaf litter, and has a global distribution. The sensitivity of A. caecus to environmental contaminants, such as neonicotinoid insecticides, as well as its life history and optimal culturing conditions, are largely unknown. The present study describes the establishment of a pure A. caecus laboratory culture and characterization of its life cycle and culturing conditions. We assessed the sensitivity of A. caecus to various insecticides, including exposures to the neonicotinoid thiamethoxam in soil and through a novel feeding assay as well as to clothianidin and cyantraniliprole in spiked soil exposures. In 7- and 14-day exposures to thiamethoxam in agricultural soil, the 50% lethal concentration (LC50) values were determined to be 0.129 mg/kg dry weight and 0.010 mg/kg dry weight, respectively. The 14-day LC50 for exposure to thiamethoxam via spiked food was determined to be 0.307 mg/kg dry weight. In addition, the 28-day 50% effect concentration for inhibition of juvenile production from cyantraniliprole exposure in the same soil type was 0.055 mg/kg dry weight. Challenges encountered in using this species included susceptibility to mite infestation and low adult survival rates in the 28-day cyantraniliprole test. Environ Toxicol Chem 2024;00:1-16. © 2024 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

Effect of Insecticide Exposure Across Multiple Generations of the Earthworm Eisenia andrei.

The toxicity of neonicotinoids and many of their replacement insecticides to nontarget soil invertebrates such as earthworms has previously been established. However, the long-term effects of these substances on these organisms are largely unknown. In the field of soil ecotoxicology, lumbricid earthworms such as Eisenia andrei are used extensively due to the availability of standardized test methods and their adaptability to laboratory culture and testing. Multigenerational studies have gained popularity and attention in recent years, with a shift toward the use of long-term assays and lower concentrations of test chemicals. The use of exposure concentrations that include those measured in a monitoring program carried out by the Government of Ontario presents a realistic exposure scenario that may not show significant effects in contemporary, shorter term studies. We used current standardized test methods as a basis for the development of multigenerational studies on E. andrei. The effects of exposure to a single application of the insecticides thiamethoxam and cyantraniliprole on the survival and reproduction of E. andrei were observed over three (thiamethoxam) or two (cyantraniliprole) generations using consecutive reproduction tests. No significant impacts on adult survival were reported in any generation for either insecticide, whereas reproduction decreased between the first and second generations in the thiamethoxam test, with median effective concentration (EC50) values of 0.022 mg/kg dry weight reported for the first generation compared with 0.002 mg/kg dry weight in the second generation. For cyantraniliprole, an EC50 of 0.064 was determined for the first generation compared with 0.016 mg/kg dry weight in the second generation. A third generation was completed for the thiamethoxam test, and a significant decrease in reproduction was observed in all treatments and controls compared with previous generations. No significant difference between thiamethoxam treatments and the control treatment was reported for the third generation. Collectively, these data indicate that exposure of oligochaetes to these two insecticides at concentrations representative of field conditions may result in long-term stresses. Environ Toxicol Chem 2024;00:1-13. © 2024 The Author(s). Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

Sediment Toxicity Tests: A Critical Review of Their use in Environmental Regulations.

Sediments are an integral component of aquatic systems, linking multiple water uses, functions, and services. Contamination of sediments by chemicals is a worldwide problem, with many jurisdictions trying to prevent future pollution (prospective) and manage existing contamination (retrospective). The present review assesses the implementation of sediment toxicity testing in environmental regulations globally. Currently, the incorporation of sediment toxicity testing in regulations is most common in the European Union (EU), North America, and Australasian regions, with some expansion in Asia and non-EU Europe. Employing sediment toxicity testing in prospective assessments (i.e., before chemicals are allowed on the market) is most advanced and harmonized with pesticides. In the retrospective assessment of environmental risks (i.e., chemicals already contaminating sediments), regulatory sediment toxicity testing practices are applied inconsistently on the global scale. International harmonization of sediment toxicity tests is considered an asset and has been successful through the widespread adoption and deployment of Organisation for Economic Co-operation and Development guidelines. On the other hand, retrospective sediment assessments benefit from incorporating regional species and protocols. Currently used toxicity testing species are diverse, with temperate species being applied most often, whereas test protocols are insufficiently flexible to appropriately address the range of environmental contaminants, including nanomaterials, highly hydrophobic contaminants, and ionized chemicals. The ever-increasing and -changing pressures placed on aquatic resources are a challenge for protection and management efforts, calling for continuous sediment toxicity test method improvement to insure effective use in regulatory frameworks. Future developments should focus on including more subtle and specific toxicity endpoints (e.g., incorporating bioavailability-based in vitro tests) and genomic techniques, extending sediment toxicity testing from single to multispecies approaches, and providing a better link with ecological protection goals. Environ Toxicol Chem 2024;00:1-20. © 2024 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

New insights into pesticide occurrence and multicompartmental monitoring strategies in stream ecosystems using periphyton and suspended sediment.

Streams are susceptible to pesticide pollutants which are transported outside of the intended area of application from surrounding agricultural fields. It is essential to monitor the occurrence and levels of pesticides in aquatic ecosystems to comprehend their effects on the aquatic environment. The common sampling strategy used for monitoring pesticides in stream ecosystems is through the collection and analysis of grab water samples. However, grab water sampling may not effectively monitor pesticides due to its limited ability to capture temporal and spatial variability, potentially missing fluctuations and uneven distribution of pesticides in aquatic environments. Monitoring using periphyton and sediment sampling may offer a more comprehensive approach by accounting for accumulative processes and temporal variations. Periphyton are a collective of microorganisms that grow on hard surfaces in aquatic ecosystems. They are responsive to chemical and biological changes in the environment, and therefore have the potential to act as a cost-effective, integrated sampling tool to monitor pesticide exposures in aquatic ecosystems. The objective of this study was to assess pesticides detected through periphyton, suspended sediment, and conventional grab water sampling methods and identify the matrix that offers a more comprehensive characterization of a stream's pesticide exposure profile. Ten streams across Southern Ontario were sampled in 2021 and 2022. At each stream site, water, sediment and periphyton, colonizing both artificial and natural substrates, were collected and analyzed for the presence of ~500 pesticides. Each of the three matrices detected distinctive pesticide exposure profiles. The frequency of detection in periphyton, sediment and water matrices were related to pesticides' log Kow and log Koc (P < 0.05). In addition, periphyton bioconcentrated 22 pesticides above levels observed in the ambient water. The bioconcentration factors of pesticides in periphyton can be predicted from their log Kow (simple linear regressions, P < 0.05). The results demonstrate that sediment and periphyton accumulate pesticides in stream environments. This highlights the importance of monitoring pesticide exposure using these matrices to ensure a complete and comprehensive characterization of exposure in stream ecosystems.

Design of tumor biomarker-monitoring trials: a proposal by the European Group on Tumor Markers.

A major application of tumor biomarkers is in serial monitoring of cancer patients, but there are no published guidelines on how to evaluate biomarkers for this purpose. The European Group on Tumor Markers has convened a multidisciplinary panel of scientists to develop guidance on the design of such monitoring trials. The panel proposes a 4-phase model for biomarker-monitoring trials analogous to that in use for the investigation of new drugs. In phase I, biomarker kinetics and correlation with tumor burden are assessed. Phase II evaluates the ability of the biomarker to identify, exclude, and/or predict a change in disease status. In phase III, the effectiveness of tumor biomarker-guided intervention is assessed by measuring patient outcome in randomized trials. Phase IV consists of an audit of the long-term effects after biomarker monitoring has been included into standard patient care. Systematic well-designed evaluations of biomarkers for monitoring may provide a stronger evidence base that might enable their earlier use in evaluating responses to cancer therapy.

Contaminants in stream sediments from seven United States metropolitan areas: part II--sediment toxicity to the amphipod Hyalella azteca and the midge Chironomus dilutus.

Relationships between sediment toxicity and sediment chemistry were evaluated for 98 samples collected from seven metropolitan study areas across the United States. Sediment-toxicity tests were conducted with the amphipod Hyalella azteca (28 day exposures) and with the midge Chironomus dilutus (10 day exposures). Overall, 33 % of the samples were toxic to amphipods and 12 % of the samples were toxic to midge based on comparisons with reference conditions within each study area. Significant correlations were observed between toxicity end points and sediment concentrations of trace elements, polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), or organochlorine (OC) pesticides; however, these correlations were typically weak, and contaminant concentrations were usually below sediment-toxicity thresholds. Concentrations of the pyrethroid bifenthrin exceeded an estimated threshold of 0.49 ng/g (at 1 % total organic carbon) in 14 % of the samples. Of the samples that exceeded this bifenthrin toxicity threshold, 79 % were toxic to amphipods compared with 25 % toxicity for the samples below this threshold. Application of mean probable effect concentration quotients (PECQs) based on measures of groups of contaminants (trace elements, total PAHs, total PCBs, OC pesticides, and pyrethroid pesticides [bifenthrin in particular]) improved the correct classification of samples as toxic or not toxic to amphipods compared with measures of individual groups of contaminants.