Identification of Polynesian mtDNA haplogroups in remains of Botocudo Amerindians from Brazil.

There is a consensus that modern humans arrived in the Americas 15,000-20,000 y ago during the Late Pleistocene, most probably from northeast Asia through Beringia. However, there is still debate about the time of entry and number of migratory waves, including apparent inconsistencies between genetic and morphological data on Paleoamericans. Here we report the identification of mitochondrial sequences belonging to haplogroups characteristic of Polynesians in DNA extracted from ancient skulls of the now extinct Botocudo Indians from Brazil. The identification of these two Polynesian haplogroups was confirmed in independent replications in Brazil and Denmark, ensuring reliability of the data. Parallel analysis of 12 other Botocudo individuals yielded only the well-known Amerindian mtDNA haplogroup C1. Potential scenarios to try to help understand these results are presented and discussed. The findings of this study may be relevant for the understanding of the pre-Columbian and/or post-Columbian peopling of the Americas.

Obesity, hypertension, social determinants of health and the epidemiologic transition among traditional Amazonian populations.

BACKGROUND: The health and nutritional situation of adults from three rural vulnerable Amazonian populations are investigated in relation to the Social Determinants of Health (SDH) and the epidemiologic transition. AIM: To investigate the role of the environment and the SDH on the occurrence of chronic-degenerative diseases in these groups. SUBJECTS AND METHODS: Anthropometric, blood pressure and demographic data were collected in adults from the RDS Mamirauá, AM (n = 149), Flona Caxiuanã, PA (n = 148) and quilombolas, PA (n = 351), populations living in a variety of socio-ecological environments in the Brazilian Amazon. RESULTS: Adjusting for the effect of age, quilombola men are taller (F = 9.85; p < 0.001) and quilombola women present with higher adiposity (F = 20.43; p < 0.001) and are more overweight/obese. Men from Mamirauá present higher adiposity (F = 9.58; p < 0.001). Mamirauá women are taller (F = 5.55; p < 0.01) and have higher values of waist circumference and subscapular/triceps index. Quilombolas present higher prevalence of hypertension in both sexes and there are significant differences in rates of hypertension among the women (χ(2) = 17.45; p < 0.01). The quilombolas are more dependent on government programmes, people from Mamirauá have more economic resources and the group from Caxiunã have the lowest SES. CONCLUSION: In these populations, the SDH play a key role in the ontogeny of diseases and the 'diseases of modernity' occur simultaneously with the always present infectoparasitic pathologies, substantially increasing social vulnerability.

Ways and detours in guarantee of health for the black population and the confrontation of racism in Brazil. / (Des)caminhos na garantia da saúde da população negra e no enfrentamento ao racismo no Brasil.

Institutional racism is prevalent in the health services in Brazil and is based on concrete power relations that subjugate, dominate and exclude blacks from having adequate access to health care and health institutions. This critical essay analyzes the importance of expanding the debate, and the production of knowledge about the health of the black population (HBP), focusing on two points: the role of the National Policy for the Integral Health of the Black Population (PNSIPN) and the importance of including the skin color item in the health information systems; and the need for a process of permanent training of professionals, including contents related to the understanding of racism as an element of the social determination of health/disease and heir effects. To demonstrate how structural and institutional racism have affected the black population, we bring also examples of the quilombola populations in the context of the Covid-19 pandemic in the country since 2020. It is concluded that the promotion of care, the reduction of inequities and the quality of health care need to undergo changes in several dimensions, such as the strengthening of the SUS, the daily fight against structural and institutional racism, among others.

O racismo institucional impera nos serviços de saúde no Brasil, fundados em relações concretas de poder que subjugam, dominam e excluem negros/as do adequado acesso aos serviços e instituições de saúde. Este ensaio crítico analisa a importância da ampliação do debate e da produção do conhecimento sobre a saúde da população negra (SPN), focando dois pontos: o papel da Política Nacional de Saúde Integral da População Negra (PNSIPN) e a importância da inserção do quesito cor nos sistemas de informação em saúde; e a necessidade de um processo de formação permanente dos/as profissionais, inserindo conteúdos relacionados à compreensão do racismo como um dos elementos de determinação social de saúde/doença e seus efeitos. Para demonstrar como o racismo estrutural e institucional tem afetado a população negra, trazemos também exemplos das populações quilombolas no contexto da pandemia de COVID-19 no país a partir de 2020. Conclui-se que a promoção do cuidado, a redução das iniquidades e a qualidade da atenção à saúde precisam passar por mudanças em várias dimensões, como o fortalecimento do SUS e o combate cotidiano ao racismo estrutural e institucional.

Late Holocene dietary and cultural variability on the Xingu River, Amazon Basin: A stable isotopic approach.

Although once considered a 'counterfeit paradise', the Amazon Basin is now a region of increasing interest in discussions of pre-colonial tropical land-use and social complexity. Archaeobotany, archaeozoology, remote sensing and palaeoecology have revealed that, by the Late Holocene, populations in different parts of the Amazon Basin were using various domesticated plants, modifying soils, building earthworks, and even forming 'Garden Cities' along the Amazon River and its tributaries. However, there remains a relatively limited understanding as to how diets, environmental management, and social structures varied across this vast area. Here, we apply stable isotope analysis to human remains (n = 4 for collagen, n = 17 for tooth enamel), and associated fauna (n = 61 for collagen, n = 28 for tooth enamel), to directly determine the diets of populations living in the Volta Grande do Rio Xingu, an important region of pre-Columbian cultural interactions, between 390 cal. years BC and 1,675 cal. years AD. Our results highlight an ongoing dietary focus on C3 plants and wild terrestrial fauna and aquatic resources across sites and time periods, with varying integration of C4 plants (i.e. maize). We argue that, when compared to other datasets now available from elsewhere in the Amazon Basin, our study highlights the development of regional adaptations to local watercourses and forest types.

HTLV-1 and HTLV-2 Infection Among Warao Indigenous Refugees in the Brazilian Amazon: Challenges for Public Health in Times of Increasing Migration.

INTRODUCTION: Human T-lymphotropic virus (HTLV) infection is endemic in indigenous populations of the Americas. We describe herein the prevalence of HTLV-1 and HTLV-2 infection among Warao indigenous refugees from Venezuela living in Belém, Pará, Brazil. METHODS: In total, 101 individuals of both sexes (43 men and 58 women) between 18 and 77 years of age were investigated. Blood samples were collected and separated into plasma and leukocytes. Serological screening was performed using an enzyme-linked immunosorbent assay (ELISA; Murex HTLV-I+II, DiaSorin, Dartford, UK), and seropositive samples were submitted to proviral DNA extraction followed by real-time polymerase chain reaction (qPCR). A nested PCR of the env region (630 bp) followed by enzymatic digestion with XhoI was performed to identify the molecular subtype of HTLV-2, in addition to sequencing analysis of the 5'LTR-I and 5'-LTR-II regions. RESULTS: Of the 101 individuals analyzed, 3 (3.0%) were seropositive. Molecular analysis of the pol and tax genes confirmed the HTLV-1 infection in a 55-year-old woman and HTLV-2 infection in a man (68 years old) and a woman (23 years old). HTLV-2 strains were defined by enzymatic digestion as belonging to the HTLV-2b subtype. The sequencing of the 5'LTR regions confirmed the presence of subtype 2b and identified HTLV-1 as belonging to subtype 1A (Cosmopolitan) and the Transcontinental subgroup. Among the infected patients, it was possible to conduct medical interviews with two individuals after delivery of the result. One patient with HTLV-2 reported symptoms such as joint pain, foot swelling, frequent headache, dizziness and lower back pain. The HTLV-1-positive woman was diagnosed with a tumor, dementia, urinary incontinence, felt body pain, and had spots on her body. The presence of the HTLV-2b subtype highlights the prevalence of this molecular variant among indigenous South Americans, as well as the presence of HTLV-1 Transcontinental, which has a worldwide distribution. CONCLUSION: These results reveal a high prevalence of HTLV-1/2 infection among Warao immigrants, suggesting migratory flow as a virus spread mechanism among human populations and alert public authorities to the need to create epidemiological surveillance programs, public social and health policies aimed at welcoming immigrants in the Brazilian territory.

Anthropometric measurements of adolescents from two Amazonian ecosystems: variations according to seasonality.

This paper aims to describe the nutritional status of Caboclo adolescents living in two areas of the Amazon Basin. Two cross-sectional studies, the first in the dry and the second in the wet season, were carried out in two Amazonian ecosystems: the forest and black water ecosystem, and the floodplain and white water ecosystem. Measurements of weight, stature, arm circumference and triceps, subscapular and suprailiac skinfolds were performed on 247 adolescents (10-19 years of age). Nutritional status was classified using body mass index according to international criteria and the prevalence of underweight and overweight was estimated. Linear mixed effects models were used with the anthropometric measurements as dependent variables and time interval, place of residence, sex, age and stature variation as independent variables. During the wet season, the prevalence of overweight among girls was higher in the forest (42%) than in the floodplain (9%). Longitudinal linear regression models showed that the arm circumference measurement was influenced both by seasonality and location, revealing that the increment between dry and wet seasons was less pronounced in the floodplain. At the time of the study, overweight already constituted a major public health concern among girls living in the forest area. In order to develop adequate public health policies for this important segment of the Amazon population further studies are necessary to investigate the role of environment and seasonality on the growth and nutritional status of adolescents.

(Des)caminhos na garantia da saúde da população negra e no enfrentamento ao racismo no Brasil / Ways and detours in guarantee of health for the black population and the confrontation of racism in Brazil

Resumo O racismo institucional impera nos serviços de saúde no Brasil, fundados em relações concretas de poder que subjugam, dominam e excluem negros/as do adequado acesso aos serviços e instituições de saúde. Este ensaio crítico analisa a importância da ampliação do debate e da produção do conhecimento sobre a saúde da população negra (SPN), focando dois pontos: o papel da Política Nacional de Saúde Integral da População Negra (PNSIPN) e a importância da inserção do quesito cor nos sistemas de informação em saúde; e a necessidade de um processo de formação permanente dos/as profissionais, inserindo conteúdos relacionados à compreensão do racismo como um dos elementos de determinação social de saúde/doença e seus efeitos. Para demonstrar como o racismo estrutural e institucional tem afetado a população negra, trazemos também exemplos das populações quilombolas no contexto da pandemia de COVID-19 no país a partir de 2020. Conclui-se que a promoção do cuidado, a redução das iniquidades e a qualidade da atenção à saúde precisam passar por mudanças em várias dimensões, como o fortalecimento do SUS e o combate cotidiano ao racismo estrutural e institucional.

Abstract Institutional racism is prevalent in the health services in Brazil and is based on concrete power relations that subjugate, dominate and exclude blacks from having adequate access to health care and health institutions. This critical essay analyzes the importance of expanding the debate, and the production of knowledge about the health of the black population (HBP), focusing on two points: the role of the National Policy for the Integral Health of the Black Population (PNSIPN) and the importance of including the skin color item in the health information systems; and the need for a process of permanent training of professionals, including contents related to the understanding of racism as an element of the social determination of health/disease and heir effects. To demonstrate how structural and institutional racism have affected the black population, we bring also examples of the quilombola populations in the context of the Covid-19 pandemic in the country since 2020. It is concluded that the promotion of care, the reduction of inequities and the quality of health care need to undergo changes in several dimensions, such as the strengthening of the SUS, the daily fight against structural and institutional racism, among others.

Da Amazônia ao guia: os dilemas entre a alimentação quilombola e as recomendações do guia alimentar para a população brasileira / From the Amazon to the guidelines: the dilemmas between quilombola foods and the recommendations of the dietary guidelines for the Brazilian population

Resumo O objetivo deste trabalho é analisar se o hábito alimentar das famílias quilombolas paraenses segue as orientações do Guia Alimentar para a População Brasileira, publicado pelo Ministério da Saúde, em 2014. Foram realizadas entrevistas semiestruturadas nas comunidades de Santo Antônio (Concórdia do Pará, nordeste paraense) e São João (Salvaterra, ilha do Marajó) sob o protocolo CEP 060/07. Foram feitas análises do consumo e das preferências alimentares de acordo com o guia. Os resultados evidenciam: alto consumo de café adoçado, feijão, arroz e farinha; baixa participação de verduras, legumes e frutas na dieta dos entrevistados. Alimentos como pão, leite de vaca, macarrão, margarina e bolacha salgada são as formas comuns de diversificar os alimentos consumidos pelo grupo. Como fontes proteicas, destacam-se a carne vermelha - silvestre ou não -, o pescado, o charque, o frango e o ovo de galinha. São apresentadas algumas contradições do guia quando aplicado às comunidades. Apesar dos avanços do guia, conclui-se que o cumprimento das orientações para uma alimentação adequada e saudável nos grupos quilombolas da região amazônica enfrenta desafios. Guias alimentares são importantes para a saúde e a nutrição da população, porém precisam ser combinados com outros tipos de intervenções que respeitem a diversidade cultural do país.

Abstract The objective of this study is to analyze the food habit of quilombola families in Pará, following the guidelines of the Food Guide for the Brazilian Population published by the Brazilian Health Ministry in 2014. Semi-structured interviews were conducted in the communities of Santo Antônio (Concórdia do Pará, in the Northeast) and São João (Salvaterra, on Marajó Island) under protocol CEP 060/07. Analysis of consumption and preferences were made according to the guide. The results show a high consumption of sweetened coffee, beans, rice and flour, and a low participation of vegetables and fruits in the interviewees' diet. Foods such as bread, cow's milk, pasta, margarine, and salt crackers are common means to diversify the foods consumed by the group. As protein sources, the most notable were red meat - wild or not -, fish, beef jerky, chicken and chicken eggs. Some contradictions of the Guide are discussed in relation to such communities. Despite the advancements promoted by the guide, we conclude that following the guidelines for an adequate and healthy food habit in quilombola groups in the Amazon region presents many challenges. Food guides are important for the health and nutrition of the population; however, they need to be combined with other types of interventions that respect the country's cultural diversity.

Subsistence patterns and blood pressure variation in two rural Caboclo communities of Marajó Island, Pará, Brazil.

Blood pressure (BP) increases with age in westernized societies, is higher in men, and is correlated with the body mass index (BMI). Traditional societies present more variable patterns of BP. In 1991, BP and anthropometric data from two "Caboclo" (rural populations of mixed ancestry) groups from Marajó Island, Brazil, were collected: The Paricatuba group, (N = 20;12 women), with a subsistence base of fishing, collection of palm fruits, and traditional gardening; and the Praia Grande group (N = 26; 14 women), where subsistence is based on mechanized agriculture. In Paricatuba, mean BP is 109/74 mmHg in men and 101/70 mmHg in women. There are no significant differences between BP of men and women, and systolic blood pressure (SBP) increases with age. Both SBP and diastolic blood pressure (DBP) are associated with weight, but only DBP is associated with the BMI, while SBP is associated with stature. In Praia Grande, mean BP is 120/76 mmHg in men and 118/70 mmHg in women, with no significant differences between the sexes. In Praia Grande, SBP is higher than in Paricatuba, and both SBP and DBP are associated with age. Compared with urban groups, both Caboclo samples have low BP. Still, differences in BP and body habitus between the two groups support a hypothesis that degree of westernization influences mean levels of BP in rural Amazonian populations. Further, the results also may be interpreted as suggesting that associations of sex, age, and BMI with BP, commonly reported in urban samples, are a byproduct of westernization rather than a result of genetic factors. © 1995 Wiley-Liss, Inc.

O Projeto Mais Médicos para o Brasil: desafios e contribuições à Atenção Básica na visão dos médicos cooperados / The More Doctors for Brazil Project - challenges and contributions to Primary Care in the vision of the cooperated physicians / El Proyecto Más Médicos para Brasil ­ Desafíos y contribuciones para la Atención Básica en la visión de los médicos cooperados

O Projeto Mais Médicos para o Brasil (PMMB) foi criado em 2013 visando prover médicos para áreas de difícil fixação de profissionais e oferecer treinamento em Saúde da Família para médicos brasileiros e estrangeiros. Neste artigo, são analisados aspectos relacionados às motivações, ao processo e condições de trabalho na Atenção Básica, à situação de saúde nos municípios, ao funcionamento do Sistema Único de Saúde (SUS) e à relação com os gestores, a partir de entrevistas com 44 médicos cubanos, trabalhando em 32 municípios de todas as regiões do Brasil. As falas mostram que os cooperados têm um agudo senso de observação e conseguem fazer um detalhado diagnóstico situacional de suas áreas de atuação, demonstrando a precariedade ainda prevalente na Atenção Básica nos municípios. Porém, eles também evidenciam que, com a adequada formação, é possível fazer atenção primária de qualidade, mesmo diante de grandes adversidades.(AU)

The More Doctors in Brazil Project (MDBP) was created in 2013 in order to supply physicians for areas where it is difficult to retain professionals and to provide training in family and community medicine for Brazilian and foreign physicians. This paper examines aspects related to motivations and work processes and conditions in primary health care; the health situation in cities, operation of the of the Brazilian National Health System (SUS), and the relationship with managers. This study is based on interviews with 44 Cuban physicians who are working in 32 cities in all regions of the country. The results showed that the affiliated physicians have an acute sense of observation and are able to make a detailed situational analysis of the areas where they work. The findings also indicated that primary care is still precarious in these cities. However, they also demonstrated that with adequate training it is possible to provide quality primary care, even in the midst of major challenges.(AU)

El Proyecto Más Médicos para Brasil (PMMB) fue creado en 2013 con el objetivo de proporcionar médicos para áreas en donde es difícil la fijación de profesionales y ofrecer capacitación en Salud de la Familia para médicos brasileños y extranjeros. En este artículo se analizan aspectos relacionados a las motivaciones, al proceso y condiciones de trabajo en la Atención Básica, a la situación de salud en los municipios, al funcionamiento del Sistema Brasileño de Salud (SUS) y a la relación con los gestores a partir de entrevistas con 44 médicos cubanos que trabajan en 32 municipios de todas las regiones del país. Los diálogos muestran que los cooperados tienen un aguzado sentido de observación y consiguen hacer un diagnóstico detallado de la situación de las áreas en las que actúan, mostrando la precariedad todavía prevalente en la Atención Básica en los municipios. No obstante, ellos también dejan claro que, con una formación adecuada, es posible proporcionar atención primaria de calidad, incluso enfrentando grandes adversidades.(AU)

Possíveis impactos na saúde de idosos institucionalizados pelo seu afastamento do convívio familiar / Possible impacts on the health of institutionalized elderly by their family removal

Este estudo apresenta algumas perspectivas sobre a influência do afastamento do convívio familiar na percepção de saúde de idosos institucionalizados, no estado do Pará, Amazônia, Brasil. Trata-se de um estudo observacional e descritivo, em que foi realizada entrevista aberta individual, e os dados foram analisados segundo a técnica de análise de discurso. Conclui-se que a autoavaliação de saúde dos idosos institucionalizados é afetada por sua percepção sobre o afastamento do convívio familiar.

This study presents some perspectives on the influence of the removal of family life on the self-assessment of health of institutionalized elderly in Pará, Amazônia, Brazil. This is an observational and descriptive study, conducted through individual open interviews. Data were analyzed according to the technique of discourse analysis. It is concluded that the self perceived health of institutionalized elders is affected by their perception of the distance from family life.

Recovering mitochondrial DNA lineages of extinct Amerindian nations in extant homopatric Brazilian populations.

BACKGROUND: Brazilian Amerindians have experienced a drastic population decrease in the past 500 years. Indeed, many native groups from eastern Brazil have vanished. However, their mitochondrial mtDNA haplotypes, still persist in Brazilians, at least 50 million of whom carry Amerindian mitochondrial lineages. Our objective was to test whether, by analyzing extant rural populations from regions anciently occupied by specific Amerindian groups, we could identify potentially authentic mitochondrial lineages, a strategy we have named 'homopatric targeting'. RESULTS: We studied 173 individuals from Queixadinha, a small village located in a territory previously occupied by the now extinct Botocudo Amerindian nation. Pedigree analysis revealed 74 unrelated matrilineages, which were screened for Amerindian mtDNA lineages by restriction fragment length polymorphism. A cosmopolitan control group was composed of 100 individuals from surrounding cities. All Amerindian lineages identified had their hypervariable segment HVSI sequenced, yielding 13 Amerindian haplotypes in Queixadinha, nine of which were not present in available databanks or in the literature. Among these haplotypes, there was a significant excess of haplogroup C (70%) and absence of haplogroup A lineages, which were the most common in the control group. The novelty of the haplotypes and the excess of the C haplogroup suggested that we might indeed have identified Botocudo lineages. To validate our strategy, we studied teeth extracted from 14 ancient skulls of Botocudo Amerindians from the collection of the National Museum of Rio de Janeiro. We recovered mtDNA sequences from all the teeth, identifying only six different haplotypes (a low haplotypic diversity of 0.8352 ± 0.0617), one of which was present among the lineages observed in the extant individuals studied. CONCLUSIONS: These findings validate the technique of homopatric targeting as a useful new strategy to study the peopling and colonization of the New World, especially when direct analysis of genetic material is not possible.

Os Objetivos de Desenvolvimento do Milênio no milênio dos idosos / The Millennium Development Goals in the millennium of the elderly

Os “Objetivos do Desenvolvimento do Milênio” (ODM) sintetizam oito compromissos assumidos internacionalmente para a melhoria da qualidade de vida no planeta até 2015. Tais expectativas, contudo, desconsideram que há um expressivo contingente de idosos vulneráveis. Uma leitura compreensiva e consciente do padrão populacional que emerge permite, contudo, que se incluam diretrizes e ações voltadas para eles, tornando urgente o planejamento integrado de políticas que atendam às precisões dos maiores de 60 anos, não só para hoje, mas para muitas décadas futuras...

The "Millennium Development Goals" (MDG) synthetize eight objectives assumed internationally to the improvement of the quality of life on this planet until 2015. Such expectations, however, do not take into consideration that there is a substantial contingent of vulnerable elderly. Even though the MDG do not address directly the elderly, a comprehensive reading, considering the emerging populational pattern, allows the inclusion of directives and actions geared to them, making urgent the integrated planning of public policies to attend the needs of those 60 years of age or older, not only today, but for the many decades to come...