Unraveling the ecological threads: How invasive alien plants influence soil carbon dynamics.

Invasive alien plants (IAPs) pose significant threats to native ecosystems and biodiversity worldwide. However, the understanding of their precise impact on soil carbon (C) dynamics in invaded ecosystems remains a crucial area of research. This review comprehensively explores the mechanisms through which IAPs influence soil C pools, fluxes, and C budgets, shedding light on their effects and broader consequences. Key mechanisms identified include changes in litter inputs, rates of organic matter decomposition, alterations in soil microbial communities, and shifts in nutrient cycling, all driving the impact of IAPs on soil C dynamics. These mechanisms affect soil C storage, turnover rates, and ecosystem functioning. Moreover, IAPs tend to increase gross primary productivity and net primary productivity leading to the alterations in fluxes and C budgets. The implications of IAP-induced alterations in soil C dynamics are significant and extend to plant-soil interactions, ecosystem structure, and biodiversity. Additionally, they have profound consequences for C sequestration, potentially impacting climate change mitigation. Restoring native plant communities, promoting soil health, and implementing species-specific management are essential measures to significantly mitigate the impacts of IAPs on soil C dynamics. Overall, understanding and mitigating the effects of IAPs on soil C storage, nutrient cycling, and related processes will contribute to the conservation of native biodiversity and complement global C neutrality efforts.

The toxicological effect on pak choi of co-exposure to degradable and non-degradable microplastics with oxytetracycline in the soil.

Microplastics and antibiotics are emerging as ubiquitous contaminants in farmland soil, harming crop quality and yield, and thus threatening global food security and human health. However, few studies have examined the individual and joint effects of degradable and/or non-degradable microplastics and antibiotics on crop plants. This study examined the individual and joint effects of polyethylene (PE) and polylactic acid (PLA) microplastics and the antibiotic oxytetracycline (OTC) on pak choi by measuring its growth, photosynthesis, antioxidant enzyme activity, and metabolite levels. Microplastics and/or oxytetracycline adversely affected root weight, photosynthesis, and antioxidant enzyme (superoxide dismutase, catalase, and ascorbate peroxidase) activities. The levels of leaf metabolites were significantly altered, causing physiological changes. Biosynthesis of plant secondary metabolites and amino acids was altered, and plant hormones pathways were disrupted. Separately and together, OTC, PE, and PLA exerted phytotoxic and antagonistic effects on pak choi. Separately and together with OTC, degradable microplastics altered the soil properties, thus causing more severe impacts on plant performance than non-degradable microplastics. This study elucidates the effects on crop plants of toxicity caused by co-exposure to degradable or non-degradable microplastic and antibiotics contamination and suggests mechanisms.

Forest biomass carbon sinks in East Asia, with special reference to the relative contributions of forest expansion and forest growth.

Forests play an important role in regional and global carbon (C) cycles. With extensive afforestation and reforestation efforts over the last several decades, forests in East Asia have largely expanded, but the dynamics of their C stocks have not been fully assessed. We estimated biomass C stocks of the forests in all five East Asian countries (China, Japan, North Korea, South Korea, and Mongolia) between the 1970s and the 2000s, using the biomass expansion factor method and forest inventory data. Forest area and biomass C density in the whole region increased from 179.78 × 10(6) ha and 38.6 Mg C ha(-1) in the 1970s to 196.65 × 10(6) ha and 45.5 Mg C ha(-1) in the 2000s, respectively. The C stock increased from 6.9 Pg C to 8.9 Pg C, with an averaged sequestration rate of 66.9 Tg C yr(-1). Among the five countries, China and Japan were two major contributors to the total region's forest C sink, with respective contributions of 71.1% and 32.9%. In China, the areal expansion of forest land was a larger contributor to C sinks than increased biomass density for all forests (60.0% vs. 40.0%) and for planted forests (58.1% vs. 41.9%), while the latter contributed more than the former for natural forests (87.0% vs. 13.0%). In Japan, increased biomass density dominated the C sink for all (101.5%), planted (91.1%), and natural (123.8%) forests. Forests in South Korea also acted as a C sink, contributing 9.4% of the total region's sink because of increased forest growth (98.6%). Compared to these countries, the reduction in forest land in both North Korea and Mongolia caused a C loss at an average rate of 9.0 Tg C yr(-1), equal to 13.4% of the total region's C sink. Over the last four decades, the biomass C sequestration by East Asia's forests offset 5.8% of its contemporary fossil-fuel CO2 emissions.

Photosynthetic responses of Larix kaempferi and Pinus densiflora seedlings are affected by summer extreme heat rather than by extreme precipitation.

The frequency and intensity of summer extreme climate events are increasing over time, and have a substantial negative effect on plants, which may be evident in their impact on photosynthesis. Here, we examined the photosynthetic responses of Larix kaempferi and Pinus densiflora seedlings to extreme heat (+ 3 °C and + 6 °C), drought, and heavy rainfall by conducting an open-field multifactor experiment. Leaf gas exchange in L. kaempferi showed a decreasing trend under increasing temperature, showing a reduction in the stomatal conductance, transpiration rate, and net photosynthetic rate by 135.2%, 102.3%, and 24.8%, respectively, in the + 6 °C treatment compared to those in the control. In contrast, P. densiflora exhibited a peak function in the stomatal conductance and transpiration rate under + 3 °C treatment. Furthermore, both species exhibited increased total chlorophyll contents under extreme heat conditions. However, extreme precipitation had no marked effect on photosynthetic activities, given the overall favorable water availability for plants. These results indicate that while extreme heat generally reduces photosynthesis by triggering stomatal closure under high vapor pressure deficit, plants employ diverse stomatal strategies in response to increasing temperature, which vary among species. Our findings contribute to the understanding of mechanisms underlying the photosynthetic responses of conifer seedlings to summer extreme climate events.

Experimental warming studies on tree species and forest ecosystems: a literature review.

Temperature affects a cascade of ecological processes and functions of forests. With future higher global temperatures being inevitable it is critical to understand and predict how forest ecosystems and tree species will respond. This paper reviews experimental warming studies in boreal and temperate forests or tree species beyond the direct effects of higher temperature on plant ecophysiology by scaling up to forest level responses and considering the indirect effects of higher temperature. In direct response to higher temperature (1) leaves emerged earlier and senesced later, resulting in a longer growing season (2) the abundance of herbivorous insects increased and their performance was enhanced and (3) soil nitrogen mineralization and leaf litter decomposition were accelerated. Besides these generalizations across species, plant ecophysiological traits were highly species-specific. Moreover, we showed that the effect of temperature on photosynthesis is strongly dependent on the position of the leaf or plant within the forest (canopy or understory) and the time of the year. Indirect effects of higher temperature included among others higher carbon storage in trees due to increased soil nitrogen availability and changes in insect performance due to alterations in plant ecophysiological traits. Unfortunately only a few studies extrapolated results to forest ecosystem level and considered the indirect effects of higher temperature. Thus more intensive, long-term studies are needed to further confirm the emerging trends shown in this review. Experimental warming studies provide us with a useful tool to examine the cascade of ecological processes in forest ecosystems that will change with future higher temperature.

High concentrations of single-walled carbon nanotubes lower soil enzyme activity and microbial biomass.

Nanomaterials such as single-walled carbon nanotubes (SWCNTs) may enter the soil environment with unknown consequences resulting from the development of nanotechnology for a variety of applications. We determined the effects of SWCNTs on soil enzyme activity and microbial biomass through a 3-week incubation of urban soils treated with different concentrations of SWCNTs ranging from 0 to 1000 µg g(-1) soil. The activities of cellobiohydrolase, ß-1,4-glucosidase, ß-1,4-xylosidase, ß-1,4-N-acetylglucosaminidase, L-leucine aminopeptidase, and acid phosphatase and microbial biomass were measured in soils treated with powder and suspended forms of SWCNTs. SWCNTs of concentrations at 300-1000 µg g(-1) soil significantly lowered activities of most enzymes and microbial biomass. It is noteworthy that the SWCNTs showed similar effects to that of multi-walled carbon nanotubes (MWCNTs), but at a concentration approximately 5 times lower; we suggest that this is mainly due to the higher surface area of SWCNTs than that of MWCNTs. Indeed, our results show that surface area of CNTs has significant negative relationship with relative enzyme activity and biomass, which suggests that greater microorganism-CNT interactions could increase the negative effect of CNTs on microorganisms. Current work may contribute to the preparation of a regulatory guideline for the release of CNTs to the soil environment.

Multi-site assessment of soil nitrogen stocks across temperate forests under different thinning intensities, recovery times, and site conditions.

Increasing research interests have been paid to understand the factors controlling soil nitrogen (N) stocks under diverse environmental conditions and forest thinning regimes. This study investigated soil N stocks across 13 temperate forests, each of which received three thinning intensities (unthinned control, 15-30 %, and 30-50 % basal area removals) under varying pre-treatment conditions (altitude, slope, soil pH, soil moisture, stand age, stand density, diameter at breast height, and tree height). The total N stored in the forest floor (L, F, and H layers) and mineral soils (0-10, 10-20, and 20-30 cm) was determined 1, 4, and 7 years after thinning. Given the various site conditions and thinning regimes, a standardized effect size was used to analyze the influences of thinning on N stocks. The N stocks (Mg N ha-1) of the forest floor and at 0-10, 10-20, and 20-30 cm mineral soil depths were 0.02-0.46, 0.32-3.21, 0.29-3.03, and 0.25-2.54 across all studied forests, respectively. The averaged effect sizes indicated decrease in forest floor N stocks and increase in mineral soil N stocks under thinning due to the reduced litterfall and eventual input of thinning residues. Thinning intensity negatively affected the effect sizes for the N stocks (P < 0.05), suggesting that excessively heavy thinning may be inappropriate for retaining forest soil N. However, multimodel inference showed that soil pH (relative importance = 1.00) and stand age (relative importance = 0.42) had the largest influence on the effect sizes for forest floor and mineral soil N stocks. This pattern suggests that the effects of thinning on soil N stocks might vary with pre-treatment conditions, even more than thinning intensities and recovery time; therefore, thinning to manage forest soil N should consider pre-treatment environmental conditions in addition to thinning regime.

The complete chloroplast genome of an Arctic lycophyte, Huperzia arctica (Tolm.) Sipliv. 1973 (Lycopodiaceae).

Huperzia arctica (Tolm.) Sipliv. 1973 is a lycophyte species belonging to the Lycopodiaceae family, which is widely distributed in the Arctic region of Svalbard, Norway. To determine its taxonomic position, we sequenced the chloroplast genome of H. arctica. The complete chloroplast genome of H. arctica is 153,956 bp in length with 122 annotated genes, including 87 protein-coding genes, 31 tRNA genes, and 4 rRNA genes. To evaluate its evolutionary position, we performed phylogenetic analysis using 36 conserved orthologous protein-coding gene sequences from the chloroplast genomes of H. arctica and publicly available data from other Lycopodiaceae. H. arctica formed a monophyletic group with four other Huperzia species: H. lucidula, H. serrata, H. crispata, and H. javanica. However, it appeared as a separate species with a highly supported bootstrap value.

Understanding the Impact of the COVID-19 Pandemic on the Perception and Use of Urban Green Spaces in Korea.

Faced with the prospect that the impact of the COVID-19 pandemic and climate change will be far-reaching and long-term, the international community is showing interest in urban green space (UGS) and urban green infrastructure utilization as a solution. In this study, we investigated how citizens' perceptions and use of UGS have changed during COVID-19. We also collected their ideas on how UGS can raise its usability. As a result, more people became to realize the importance of UGS. In particular, the urban environmental purification function from UGS was recognized as giving great benefits to respondents. On the other hand, the patterns of UGS use were mixed with decreasing UGS use to maintain social distancing or increasing UGS use to maintain health or substitute other restricted facilities. More than half of respondents had their UGS visit patterns impacted by COVID-19. In particular, the increase rate of UGS use was rather high in the group that seldom used UGS before COVID-19. In addition, they increased the use of UGS to replace other limited facilities, and thus tended to demand an increase in rest facilities. Based on these results, this paper suggested securing social support and sustainability for the policy by reflecting users' demand in landscape planning related to the increase of UGS in the city. This study can contribute to improving the resilience of UGS and the sustainability of urban space planning.

Tree size diversity is the major driver of aboveground carbon storage in dryland agroforestry parklands.

Despite the importance of agroforestry parkland systems for ecosystem and livelihood benefits, evidence on determinants of carbon storage in parklands remains scarce. Here, we assessed the direct and indirect influence of human management (selective harvesting of trees), abiotic factors (climate, topography, and soil) and multiple attributes of species diversity (taxonomic, functional, and structural) on aboveground carbon (AGC) stocks in 51 parklands in drylands of Benin. We used linear mixed-effects regressions and structural equation modeling to test the relative effects of these predictors on AGC stocks. We found that structural diversity (tree size diversity, HDBH) had the strongest (effect size ß = 0.59, R2 = 54%) relationship with AGC stocks, followed by community-weighted mean of maximum height (CWMMAXH). Taxonomic diversity had no significant direct relationship with AGC stocks but influenced the latter indirectly through its negative effect on CWMMAXH, reflecting the impact of species selection by farmers. Elevation and soil total organic carbon content positively influenced AGC stocks both directly and indirectly via HDBH. No significant association was found between AGC stocks and tree harvesting factor. Our results suggest the mass ratio, niche complementarity and environmental favorability as underlying mechanisms of AGC storage in the parklands. Our findings also highlight the potential role of human-driven filtering of local species pool in regulating the effect of biodiversity on AGC storage in the parklands. We conclude that the promotion of AGC stocks in parklands is dependent on protecting tree regeneration in addition to enhancing tree size diversity and managing tall-stature trees.

Structural characteristics of dissolved black carbon and its interactions with organic and inorganic contaminants: A critical review.

Biochar (BC) is a sustainable and renewable carbonaceous material, and its soluble component, dissolved black carbon (DBC), is the key to understanding BC's geological and environmental processes. Although the relationship between the changes in DBC structure and its properties, functions, and associated environmental risks has been explored, a gap remains in our understanding of DBC's fate and behavior in the natural environment. Thus, in this review, we have highlighted the molecular and chemical compositions and the structural evolution of DBC during pyrolysis, the influence of DBC's physicochemical properties on its fate and transport, DBC's interaction with soil and its contaminants, and DBC stability in soil and water environments along with potential risks. Based on our in-depth assessment of DBC and its biogeochemical roles, we believe that future studies should focus on the following: (1) using advanced techniques to understand the chemical and molecular structure of DBC deeply and concisely and, thus, determine its fundamental role in the natural environment; (2) investigating the multi-functional properties of DBC and its interaction mechanisms; and (3) evaluating the environmental behaviors of and risks associated with DBC after BC application. In future, it is necessary to gain a deeper insight into the fate and transport of DBC with contaminants and study its associated risks under BC application in the environment.

Application of integrated Korean forest growth dynamics model to meet NDC target by considering forest management scenarios and budget.

BACKGROUND: Forests are atmospheric carbon sinks, whose natural growth can contribute to climate change mitigation. However, they are also affected by climate change and various other phenomena, for example, the low growth of coniferous forests currently reported globally, including in the Republic of Korea. In response to the implementation of the Paris Agreement, the Korean government has proposed 2030 greenhouse gas roadmap to achieve a Nationally Determined Contribution (NDC), and the forest sector set a sequestration target of 26 million tons by 2030. In this study, the Korean forest growth model (KO-G-Dynamic model) was used to analyze various climate change and forest management scenarios and their capacity to address the NDC targets. A 2050 climate change adaptation strategy is suggested based on forest growth and CO2 sequestration. RESULTS: Forest growth was predicted to gradually decline, and CO2 sequestration was predicted to reach 23 million tons per year in 2050 if current climate and conditions are maintained. According to the model, sequestrations of 33 million tCO2 year-1 in 2030 and 27 million tCO2 year-1 in 2050 can be achieved if ideal forest management is implemented. It was also estimated that the current forest management budget of 317 billion KRW (264 million USD) should be twice as large at 722 billion KRW (602 million USD) in the 2030s and 618 billion KRW (516 million USD) in the 2050s to achieve NDC targets. CONCLUSIONS: The growth trend in Korea's forests transitions from young-matured stands to over-mature forests. The presented model-based forest management plans are an appropriate response and can increase the capacity of Korea to achieve its NDC targets. Such a modeling can help the forestry sector develop plans and policies for climate change adaptation.

The effect of multi-walled carbon nanotubes on soil microbial activity.

Nanomaterials such as multi-walled carbon nanotubes (MWCNTs) are applied to various industrial products and thus may be released to soils, but their potential environmental impacts remain largely undetermined. We investigated the short-term effect of MWCNTs on the activity and biomass of microorganisms inhabiting two different soil types in an incubation study. Up to 5000 µg MWCNT g(-1) soil was applied and the activities of 1,4-ß-glucosidase, cellobiohydrolase, xylosidase, 1,4-ß-N-acetylglucosaminidase, and phosphatase and microbial biomass were measured. In both soil types, most enzyme activities showed a tendency to be repressed under 500 µg MWCNT g(-1) soil, and all enzymatic activities as well as microbial biomass C and N were significantly lowered under 5000 µg MWCNT g(-1) soil. Our results suggest that high concentrations of MWCNTs could lower the microbial activity and biomass in soils, and they may serve as an important guideline in regulating the release of MWCNTs to the soil environment.

The initial effects of microclimate and invertebrate exclusion on multi-site variation in the mass loss of temperate pine and oak deadwoods.

Quantifying deadwood decomposition is prioritized by forest ecologists; nonetheless, uncertainties remain for its regional variation. This study tracked variations in deadwood decomposition of Korean red pine and sawtooth oak in three environmentally different regions of the Republic of Korea, namely western, eastern, and southern regions. After 24 months, dead pine and oak woods lost 47.3 ± 2.8% and 23.5 ± 1.6% in the southern region, 13.3 ± 2.6% and 20.2 ± 2.8% in the western region, and 11.9 ± 7.9% and 13.9 ± 2.3% in the eastern region, respectively. The regional variation in the decomposition rate was significant only for dead pine woods (P < 0.05). Invertebrate exclusion treatment reduced the decomposition rate in all region, and had the greatest effect in the southern region where warmer climate and concentrated termite colonization occurred. The strongest influential factor for the decomposition of dead pine woods was invertebrate exclusion (path coefficient: 0.63). Contrastingly, the decomposition of dead oak woods was highly controlled by air temperature (path coefficient: 0.88), without significant effect of invertebrate exclusion. These findings reflect the divergence in regional variation of deadwood decomposition between pine and oak, which might result from the different sensitivity to microclimate and decomposer invertebrates.

Carbon and nitrogen status of litterfall, litter decomposition and soil in even-aged larch, red pine and rigitaeda pine plantations.

The carbon (C) and nitrogen (N) status in forest ecosystems can change upon establishment of plantations because different tree species have different nutrient cycling mechanisms. This study was carried out to evaluate C and N status of litterfall, litter decomposition and soil in three adjacent plantations consisting of one deciduous (larch: Larix leptolepis) and two evergreen (red pine: Pinus densiflora; rigitaeda pine: P. rigida x P. taeda) species planted in the same year (1963). Both the pine plantations showed comparatively higher C input from needle litter but significantly lower N concentration and input than the larch plantation (P < 0.05). During the decomposition process, the deciduous larch needle litter showed low C concentration and C remaining in soil, but high N concentration and N remaining in soil compared to the two evergreen pine needle litters. However, the soil C and N concentration and their content at a soil depth of 0-10 cm were not affected significantly (P > 0.05) by the plantation type. These results demonstrate the existence of considerable variation in C and N status resulting from needle litter input and litter decomposition in these three plantations grown at sites with similar environmental conditions.

Autotrophic and heterotrophic respiration in needle fir and Quercus-dominated stands in a cool-temperate forest, central Korea.

To investigate annual variation in soil respiration (R (S)) and its components [autotrophic (R (A)) and heterotrophic (R (H))] in relation to seasonal changes in soil temperature (ST) and soil water content (SWC) in an Abies holophylla stand (stand A) and a Quercus-dominated stand (stand Q), we set up trenched plots and measured R (S), ST and SWC for 2 years. The mean annual rate of R (S) was 436 mg CO(2) m(-2) h(-1), ranging from 76 to 1,170 mg CO(2) m(-2) h(-1), in stand A and 376 mg CO(2) m(-2) h(-1), ranging from 82 to 1,133 mg CO(2) m(-2) h(-1), in stand Q. A significant relationship between R (S) and its components and ST was observed over the 2 years in both stands, whereas a significant correlation between R (A) and SWC was detected only in stand Q. On average over the 2 years, R (A) accounted for approximately 34% (range 17-67%) and 31% (15-82%) of the variation in R (S) in stands A and Q, respectively. Our results suggested that vegetation type did not significantly affect the annual mean contributions of R (A) or R (H), but did affect the pattern of seasonal change in the contribution of R (A) to R (S).

Development processes and growth pattern of Pinus densiflora stands in central eastern Korea.

Stand growth and developmental processes were investigated in Pinus densiflora Siebold et Zucc. stands of different ages in the central eastern region of Korea. Stands were inventoried and five trees per stand were sampled for stem analysis, age estimation, and growth analysis. More than 80% of sampled trees in a stand were established within 3-5 years, and most stands had a single cohort structure. The initial growth of pine seedlings was slow, but the height growth accelerated beyond 2-3 m height, 5-10 years after establishment. Linear growth was maintained until 10-12 m height, at which suppressed trees fell behind and might die out. The young stand was composed of pure pines, while few pine seedlings and saplings were found in the understory of older stands. The peak of diameter growth rate occurred around 5-15 years after tree establishment, implying that competition begins during that period. The pine stand development follows four stages: (1) the young stage when the growth rate increases and peaks; (2) the height competition stage when trees focus on height growth for light while maintaining a narrow DBH and height distribution; (3) the differentiation stage when suppressed trees die out, and the DBH distribution becomes wider; and (4) the mature stage when stands have a multi-canopy structure with a wide DBH and height distribution, while the understory is dominated by other tree species. The changes in growth rates and stand structure through forest development would be implemented to predict alterations of above-ground carbon sequestration rates.

Estimating stem volume and biomass of Pinus koraiensis using LiDAR data.

The objective of this study was to estimate the stem volume and biomass of individual trees using the crown geometric volume (CGV), which was extracted from small-footprint light detection and ranging (LiDAR) data. Attempts were made to analyze the stem volume and biomass of Korean Pine stands (Pinus koraiensis Sieb. et Zucc.) for three classes of tree density: low (240 N/ha), medium (370 N/ha), and high (1,340 N/ha). To delineate individual trees, extended maxima transformation and watershed segmentation of image processing methods were applied, as in one of our previous studies. As the next step, the crown base height (CBH) of individual trees has to be determined; information for this was found in the LiDAR point cloud data using k-means clustering. The LiDAR-derived CGV and stem volume can be estimated on the basis of the proportional relationship between the CGV and stem volume. As a result, low tree-density plots had the best performance for LiDAR-derived CBH, CGV, and stem volume (R (2) = 0.67, 0.57, and 0.68, respectively) and accuracy was lowest for high tree-density plots (R (2) = 0.48, 0.36, and 0.44, respectively). In the case of medium tree-density plots accuracy was R (2) = 0.51, 0.52, and 0.62, respectively. The LiDAR-derived stem biomass can be predicted from the stem volume using the wood basic density of coniferous trees (0.48 g/cm(3)), and the LiDAR-derived above-ground biomass can then be estimated from the stem volume using the biomass conversion and expansion factors (BCEF, 1.29) proposed by the Korea Forest Research Institute (KFRI).

Influence of stand density on soil CO2 efflux for a Pinus densiflora forest in Korea.

We investigated the influence of stand density [938 tree ha(-1) for high stand density (HD), 600 tree ha(-1) for medium stand density (MD), and 375 tree ha(-1) for low stand density (LD)] on soil CO(2) efflux (R (S)) in a 70-year-old natural Pinus densiflora S. et Z. forest in central Korea. Concurrent with R (S) measurements, we measured litterfall, total belowground carbon allocation (TBCA), leaf area index (LAI), soil temperature (ST), soil water content (SWC), and soil nitrogen (N) concentration over a 2-year period. The R (S) (t C ha(-1) year(-1)) and leaf litterfall (t C ha(-1) year(-1)) values varied with stand density: 6.21 and 2.03 for HD, 7.45 and 2.37 for MD, and 6.96 and 2.23 for LD, respectively. In addition, R (S) was correlated with ST (R (2) = 0.77-0.80, P < 0.001) and SWC (R (2) = 0.31-0.35, P < 0.001). It appeared that stand density influenced R (S) via changes in leaf litterfall, LAI and SWC. Leaf litterfall (R (2) = 0.71), TBCA (R (2) = 0.64-0.87), and total soil N contents in 2007 (R (2) = 0.94) explained a significant amount of the variance in R (S) (P < 0.01). The current study showed that stand density is one of the key factors influencing R (S) due to the changing biophysical and environmental factors in P. densiflora.

Microbial biomass and enzymatic responses to temperate oak and larch forest thinning: Influential factors for the site-specific changes.

Microbial biomass and enzyme activity are essential for ecosystem function in managed forests; however, uncertainty remains because microbial biomass and enzymatic responses to thinning highly differ with case studies. This study addressed the drivers for the site-specific responses of microbial biomass and enzyme activity to thinning. Study sites included two oak and three larch forests; each had un-thinned control, intermediate thinning (15-23% basal area reduction), and heavy thinning treatments (30-44% basal area reduction). Soil properties (temperature, water content, pH, total and inorganic nitrogen, and total carbon/nitrogen ratio), microbial biomass, enzyme (ß-glucosidase, N-acetylglucosaminidase, leucyl aminopeptidase, acid phosphatase, and phenol oxidase) activity, and soil carbon storage were determined 6 years after thinning. Compared to the control, microbial biomass carbon and nitrogen were higher under the intermediate and the heavy thinning by 13.9 and 24.4% and 11.5 and 29.9% at one oak forests, respectively, and higher under the intermediate thinning by 53.7 and 70.7% at one larch forests. There were the post-thinning changes in leucyl aminopeptidase activity by -46.9% and by 150.0-210.0% at an oak and larch forest, respectively, acid phosphatase activity by 60.0% at one oak forest, and phenol oxidase activity by 355.0% at one oak forest. The effect sizes of thinning for soil properties explained 94% and 77% of variance of the effect sizes for microbial biomass and enzyme activity. Especially, the effect sizes for soil water content, NH4+, total carbon/nitrogen ratio, and temperature were the most influential. Furthermore, the effect size for soil carbon storage was parabolically related to the effect size for microbial biomass carbon (R2 = 0.66). These findings highlight that inconsistent thinning effects on soil properties varied microbial biomass and enzymatic responses to thinning, which differentiated the change in soil carbon storage across sites. Future studies should consider such inconsistencies when examining the effects of forest management.