RESUMO
The development of the mammalian cerebellum is orchestrated by both cell-autonomous programs and inductive environmental influences. Here, we describe the main processes of cerebellar ontogenesis, highlighting the neurogenic strategies used by developing progenitors, the genetic programs involved in cell fate specification, the progressive changes of structural organization, and some of the better-known abnormalities associated with developmental disorders of the cerebellum.
Assuntos
Cerebelo/embriologia , Cerebelo/crescimento & desenvolvimento , Animais , Cerebelo/citologia , Cerebelo/fisiopatologia , Consenso , Humanos , Neurogênese/fisiologia , Neurônios/citologia , Neurônios/fisiologiaRESUMO
During the past 25 years, our knowledge on the development of basket and stellate cells (molecular layer interneurons [MLIs]) has completely changed, not only regarding their origin from the ventricular zone, corresponding to the primitive cerebellar neuroepithelium, instead of the external granular layer, but above all by providing an almost complete account of the genetic regulations (transcription factors and other genes) involved in their differentiation and synaptogenesis. Moreover, it has been shown that MLIs' precursors (dividing neuroblasts) and not young postmitotic neurons, as in other germinal neuroepithelia, leave the germinative zone and migrate all along a complex and lengthy path throughout the presumptive cerebellar white matter, which provides suitable niches exerting epigenetic influences on their ultimate neuronal identities. Recent studies carried out on the anatomical-functional properties of adult MLIs emphasize the importance of these interneurons in regulating PC inhibition, and point out the crucial role played by electrical synaptic transmission between MLIs as well as ephaptic interactions between them and Purkinje cells at the pinceaux level, in the regulation of this inhibition.
Assuntos
Cerebelo/citologia , Cerebelo/crescimento & desenvolvimento , Interneurônios/fisiologia , Sinapses/fisiologia , Animais , HumanosRESUMO
Neurons in the CNS of higher vertebrates lose their ability to regenerate their axons at a stage of development that coincides with peak circulating thyroid hormone (T(3)) levels. Here, we examined whether this peak in T(3) is involved in the loss of axonal regenerative capacity in Purkinje cells (PCs). This event occurs at the end of the first postnatal week in mice. Using organotypic culture, we found that the loss of axon regenerative capacity was triggered prematurely by early exposure of mouse PCs to T(3), whereas it was delayed in the absence of T(3). Analysis of mutant mice showed that this effect was mainly mediated by the T(3) receptor α1. Using gain- and loss-of-function approaches, we also showed that Krüppel-like factor 9 was a key mediator of this effect of T(3). These results indicate that the sudden physiological increase in T(3) during development is involved in the onset of the loss of axon regenerative capacity in PCs. This loss of regenerative capacity might be part of the general program triggered by T(3) throughout the body, which adapts the animal to its postnatal environment.
Assuntos
Cerebelo/fisiologia , Fatores de Transcrição Kruppel-Like/genética , Regeneração Nervosa/fisiologia , Células de Purkinje/fisiologia , Tri-Iodotironina/metabolismo , Adaptação Fisiológica/fisiologia , Animais , Axônios/fisiologia , Axotomia , Cerebelo/crescimento & desenvolvimento , Feminino , Regulação da Expressão Gênica no Desenvolvimento/fisiologia , Fatores de Transcrição Kruppel-Like/metabolismo , Lentivirus/genética , Masculino , Camundongos , Camundongos Knockout , Regeneração Nervosa/efeitos dos fármacos , Técnicas de Cultura de Órgãos , Gravidez , Células de Purkinje/efeitos dos fármacos , Receptores dos Hormônios Tireóideos/metabolismo , Tri-Iodotironina/farmacologiaRESUMO
Neuronal maturation during development is a multistep process regulated by transcription factors. The transcription factor RORα (retinoic acid-related orphan receptor α) is necessary for early Purkinje cell (PC) maturation but is also expressed throughout adulthood. To identify the role of RORα in mature PCs, we used Cre-lox mouse genetic tools in vivo that delete it specifically from PCs between postnatal days 10-21. Up to 14 d of age, differences between mutant and control PCs were not detectable: both were mono-innervated by climbing fibers (CFs) extending along their well-developed dendrites with spiny branchlets. By week 4, mutant mice were ataxic, some PCs had died, and remaining PC soma and dendrites were atrophic, with almost complete disappearance of spiny branchlets. The innervation pattern of surviving RORα-deleted PCs was abnormal with several immature characteristics. Notably, multiple functional CF innervation was reestablished on these mature PCs, simultaneously with the relocation of CF contacts to the PC soma and their stem dendrite. This morphological modification of CF contacts could be induced even later, using lentivirus-mediated depletion of rora from adult PCs. These data show that the late postnatal expression of RORα cell-autonomously regulates the maintenance of PC dendritic complexity, and the CF innervation status of the PC (dendritic vs somatic contacts, and mono-innervation vs multi-innervation). Thus, the differentiation state of adult neurons is under the control of transcription factors; and in their absence, adult neurons lose their mature characteristics and acquire some characteristics of an earlier developmental stage.
Assuntos
Fibras Nervosas/fisiologia , Membro 1 do Grupo F da Subfamília 1 de Receptores Nucleares/fisiologia , Células de Purkinje/fisiologia , Animais , Axônios/fisiologia , Axônios/ultraestrutura , Comportamento Animal/fisiologia , Contagem de Células , Diferenciação Celular/genética , Diferenciação Celular/fisiologia , DNA/genética , Fatores de Transcrição Forkhead/genética , Vetores Genéticos , Humanos , Imuno-Histoquímica , Relações Interpessoais , Lentivirus/genética , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Microscopia Eletrônica , Força Muscular/genética , Força Muscular/fisiologia , Mutação/genética , Membro 1 do Grupo F da Subfamília 1 de Receptores Nucleares/genética , Reação em Cadeia da Polimerase , Equilíbrio Postural/fisiologia , Desempenho Psicomotor/fisiologia , Proteínas Repressoras/genética , Proteína Vesicular 1 de Transporte de Glutamato/genética , Proteína Vesicular 2 de Transporte de Glutamato/genéticaRESUMO
The HERC gene family encodes proteins with two characteristic domains: HECT and RCC1-like. Proteins with HECT domains have been described to function as ubiquitin ligases, and those that contain RCC1-like domains have been reported to function as GTPases regulators. These two activities are essential in a number of important cellular processes such as cell cycle, cell signaling, and membrane trafficking. Mutations affecting these domains have been found associated with retinitis pigmentosa, amyotrophic lateral sclerosis, and cancer. In humans, six HERC genes have been reported which encode two subgroups of HERC proteins: large (HERC1-2) and small (HERC3-6). The giant HERC1 protein was the first to be identified. It has been involved in membrane trafficking and cell proliferation/growth through its interactions with clathrin, M2-pyruvate kinase, and TSC2 proteins. Mutations affecting other members of the HERC family have been found to be associated with sterility and growth retardation. Here, we report the characterization of a recessive mutation named tambaleante, which causes progressive Purkinje cell degeneration leading to severe ataxia with reduced growth and lifespan in homozygous mice aged over two months. We mapped this mutation in mouse chromosome 9 and then performed positional cloning. We found a G<-->A transition at position 1448, causing a Gly to Glu substitution (Gly483Glu) in the highly conserved N-terminal RCC1-like domain of the HERC1 protein. Successful transgenic rescue, with either a mouse BAC containing the normal copy of Herc1 or with the human HERC1 cDNA, validated our findings. Histological and biochemical studies revealed extensive autophagy associated with an increase of the mutant protein level and a decrease of mTOR activity. Our observations concerning this first mutation in the Herc1 gene contribute to the functional annotation of the encoded E3 ubiquitin ligase and underline the crucial and unexpected role of this protein in Purkinje cell physiology.
Assuntos
Mutação de Sentido Incorreto/genética , Células de Purkinje/enzimologia , Células de Purkinje/patologia , Ubiquitina-Proteína Ligases/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Mapeamento Cromossômico , Dendritos/enzimologia , Dendritos/ultraestrutura , Regulação da Expressão Gênica , Loci Gênicos/genética , Genótipo , Longevidade , Camundongos , Camundongos Mutantes Neurológicos , Dados de Sequência Molecular , Fenótipo , Células de Purkinje/ultraestrutura , Ubiquitina-Proteína Ligases/químicaRESUMO
Inferior olivary (IO) neurons are born in the dorsal hindbrain and migrate tangentially toward the ventral midline. During their dorsoventral migration, IO neurons extend long leading processes that cross the midline, transform into axons, and project into the contralateral cerebellum. In absence of the axon guidance receptor Robo3, IO axons fail to cross the midline and project to the ipsilateral cerebellum. Remarkably, the IO cell bodies still reach the midline where they form a nucleus of abnormal cytoarchitecture. The mechanisms underlying the migration of Robo3-deficient IO neurons are unknown. Here, we used three-dimensional imaging and transgenic mice to label subsets of IO neurons and study their migratory behavior in Robo3 knockout. We show that IO migration is delayed in absence of Robo3. Strikingly, Robo3-deficient IO neurons progress toward the midline in a direction opposite to their axons. This occurs through a change of polarity and the generation of a second leading process at the rear of the cell. These results suggest that Robo3 receptor controls the establishment of neuronal polarity and the coupling of axonogenesis and cell body migration in IO neurons.
Assuntos
Orientação de Axônios , Proteínas do Tecido Nervoso , Animais , Axônios/metabolismo , Movimento Celular/fisiologia , Camundongos , Camundongos Transgênicos , Proteínas do Tecido Nervoso/metabolismo , Neurogênese , Neurônios/metabolismo , Núcleo Olivar/metabolismoRESUMO
While the origin of oligodendroglia in the prosencephalon and spinal cord has been extensively studied and accurately described, the origin of this cell type in the cerebellum is largely unknown. To investigate where cerebellar oligodendrocytes generate and which migratory pathways they follow to reach their final destination in the adult, in ovo transplants were performed using the quail/chick chimeric system. The chimeric embryos were developed up to HH43-49 (17-19 days of incubation) to map the location of donor cells and analyze their phenotype by immunohistochemistry. As a result, mesencephalic homotopic and homochronic transplants generated cellular migratory streams moving from the grafted epithelium into the host cerebellum, crossing the isthmus mainly through the velum medullare and invading the central white matter. From here, these mesencephalic cells invaded all the layers of the cerebellar cortex except the granular layer. The majority of the cells were detected in the central and folial white matter, as well as in superficial regions of the internal granular layer, surrounding the Purkinje cells. In the latter case, the donor cells presented a Bergmann glial morphology and were Vimentin positive, while in other areas they were PLP and Olig2-positive, indicating an oligodendroglial fate. The combinatory analysis of the different grafts allowed us to propose the fate map of chick cerebellar oligodendroglia at the neural tube stage. As a result, the majority of the cerebellar oligodendrocytes originate from the parabasal plate of the mesencephalon.
Assuntos
Diferenciação Celular/fisiologia , Linhagem da Célula/fisiologia , Córtex Cerebelar/citologia , Mesencéfalo/citologia , Oligodendroglia/citologia , Células-Tronco/citologia , Animais , Córtex Cerebelar/embriologia , Embrião de Galinha , Galinhas , Coturnix , Mesencéfalo/embriologia , Oligodendroglia/fisiologia , Quimera por Radiação , Células-Tronco/fisiologiaRESUMO
In the last 50â¯years, our vision of the cerebellum has vastly evolved starting with Voogd's (1967) description of extracerebellar projections' terminations and how the projection maps transformed the presumptive homogeneity of the cerebellar cortex into a more complex center subdivided into transverse and longitudinal distinct functional zones. The picture became still more complex with Richard Hawkes and colleagues' (Gravel et al., 1987) discovery of the biochemical heterogeneity of Purkinje cells (PCs), by screening their molecular identities with monoclonal antibodies. Antigens were expressed in a parasagittal pattern with subsets of PCs either possessing or lacking the respective antigens, which divided the cerebellar cortex into precise longitudinal compartments that are congruent with the projection maps. The correlation of these two maps in adult cerebellum shows a perfect matching of developmental mechanisms. This review discusses a series of arguments in favor of the essential role played by PCs in organizing the microzonation of the cerebellum during development (the "matching" hypothesis).
Assuntos
Cerebelo , Células de Purkinje , Anticorpos Monoclonais , Antígenos , Córtex CerebelarRESUMO
GABAergic interneurons migrate long distances through stereotyped migration programs toward specific laminar positions. During their migration, GABAergic interneurons are morphologically alike but then differentiate into a rich array of interneuron subtypes critical for brain function. How interneuron subtypes acquire their final phenotypic traits remains largely unknown. Here, we show that cerebellar molecular layer GABAergic interneurons, derived from the same progenitor pool, use separate migration paths to reach their laminar position and differentiate into distinct basket cell (BC) and stellate cell (SC) GABAergic interneuron subtypes. Using two-photon live imaging, we find that SC final laminar position requires an extra step of tangential migration supported by a subpopulation of glutamatergic granule cells (GCs). Conditional depletion of GCs affects SC differentiation but does not affect BCs. Our results reveal how timely feedforward control of inhibitory interneuron migration path regulates their terminal differentiation and, thus, establishment of the local inhibitory circuit assembly.
Assuntos
Diferenciação Celular , Grânulos Citoplasmáticos/metabolismo , Interneurônios/citologia , Animais , Axônios/metabolismo , Movimento Celular , Neurônios GABAérgicos/citologia , Interneurônios/metabolismo , Camundongos TransgênicosRESUMO
Why did I choose this particular topic for my lecture rather than the history of neuroscience or the history of the neuron? Simply because I believe that every disciple has the obligation to pay homage to their mentors once in their lifetime. My formation as a neuroscientist involved three such mentors spanned across three countries. The first was Spain, where I was born, completed my medical studies, and had my first glimpse of neuroscience at the Cajal Institute with Fernando de Castro. It was him who, in 1961, advised me to spend some time abroad, and to that purpose he obtained me a scholarship from the French government, that allowed me to settle in Paris. Once in France I had the good fortune to meet Prof. René Couteaux, another generous mentor, who took care of my stay in the country. Two years later, he made me a proposition to which I could only answer in the affirmative by offering me a research position in France. I got married (the best thing that happened in my life), and spent the next 57 years working on the cerebellum. The third person I want to honor and remember in this presentation is Sanford Louis Palay who was my postdoc professor during the 2 years I worked at Harvard Medical School in Boston. And as it turns out, all three of my mentors have made positive contributions to the history of the synapse. So, without further delay, let us dive in. Anat Rec, 303:1252-1279, 2020. © 2020 American Association for Anatomy.
Assuntos
Encéfalo/fisiologia , Neurociências/história , Sinapses/fisiologia , Animais , História do Século XX , Humanos , Neuroglia/fisiologia , Neurônios/fisiologiaRESUMO
Precerebellar neurons of the inferior olive (IO) and lateral reticular nucleus (LRN) migrate tangentially from the rhombic lip toward the floor plate following parallel pathways. This process is thought to involve netrin-1 attraction. However, whereas the cell bodies of LRN neurons cross the midline, IO neurons are unable to do so. In many systems and species, axon guidance and cell migration at the midline are controlled by Slits and their receptor Robos. We showed previously that precerebellar axons and neurons do not cross the midline in the absence of the Robo3 receptor. To determine whether this signaling by Slits and the two other Robo receptors, Robo1 and Robo2, also regulates precerebellar neuron behavior at the floor plate, we studied the phenotype of Slit1/2 and Robo1/2/3 compound mutants. Our results showed that many IO neurons can cross the midline in absence of Slit1/2 or Robo1/2, supporting a role for midline repellents in guiding precerebellar neurons. We also show that these molecules control the development of the lamellation of the inferior olivary complex. Last, the analysis of Robo1/2/3 triple mutants suggests that Robo3 inhibits Robo1/2 repulsion in precrossing LRN axons but not in IO axons in which it has a dominant and distinct function.
Assuntos
Movimento Celular/fisiologia , Cerebelo/citologia , Cerebelo/embriologia , Neurônios/citologia , Animais , Cerebelo/metabolismo , Glicoproteínas/deficiência , Glicoproteínas/genética , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Proteínas do Tecido Nervoso/deficiência , Proteínas do Tecido Nervoso/genética , Neurônios/metabolismo , Núcleo Olivar/citologia , Núcleo Olivar/embriologia , Núcleo Olivar/metabolismo , Receptores Imunológicos/deficiência , Receptores Imunológicos/genética , Formação Reticular/citologia , Formação Reticular/embriologia , Formação Reticular/metabolismo , Proteínas RoundaboutRESUMO
During development, precerebellar neurons migrate dorsoventrally from the rhombic lip to the floor plate. Some of these neurons cross the midline while others stop. We have identified a role for the slit receptor Rig-1/Robo3 in directing this process. During their tangential migration, neurons of all major hindbrain precerebellar nuclei express high levels of Rig-1 mRNA. Rig-1 expression is rapidly downregulated as their leading process crosses the floor plate. Interestingly, most precerebellar nuclei do not develop normally in Rig-1-deficient mice, as they fail to cross the midline. In addition, inferior olivary neurons, which normally send axons into the contralateral cerebellum, project ipsilaterally in Rig-1 mutant mice. Similarly, neurons of the lateral reticular nucleus and basilar pons are unable to migrate across the floor plate and instead remain ipsilateral. These results demonstrate that Rig-1 controls the ability of both precerebellar neuron cell bodies and their axons to cross the midline.
Assuntos
Cerebelo/embriologia , Cones de Crescimento/metabolismo , Proteínas de Membrana/fisiologia , Proteínas do Tecido Nervoso/metabolismo , Proteínas do Tecido Nervoso/fisiologia , Vias Neurais/embriologia , Rombencéfalo/embriologia , Animais , Diferenciação Celular/genética , Cerebelo/citologia , Cerebelo/metabolismo , Feto , Lateralidade Funcional/genética , Regulação da Expressão Gênica no Desenvolvimento/genética , Cones de Crescimento/ultraestrutura , Proteínas de Membrana/genética , Camundongos , Camundongos Knockout , Mutação/genética , Proteínas do Tecido Nervoso/genética , Vias Neurais/citologia , Vias Neurais/metabolismo , Núcleo Olivar/citologia , Núcleo Olivar/embriologia , Núcleo Olivar/metabolismo , Ponte/citologia , Ponte/embriologia , Ponte/metabolismo , RNA Mensageiro/metabolismo , Receptores de Superfície Celular , Rombencéfalo/citologia , Rombencéfalo/metabolismoRESUMO
Cerebellar granule cell progenitors proliferate postnatally in the upper part of the external granule cell layer (EGL) of the cerebellum. Postmitotic granule cells differentiate and migrate, tangentially in the EGL and then radially through the molecular and Purkinje cell layers. The molecular control of the transition between proliferation and differentiation in cerebellar granule cells is poorly understood. We show here that the transmembrane receptor Plexin-B2 is expressed by proliferating granule cell progenitors. To study Plexin-B2 function, we generated a targeted mutation of mouse Plexin-B2. Most Plexin-B2(-/-) mutants die at birth as a result of neural tube closure defects. Some mutants survive but their cerebellum cytoarchitecture is profoundly altered. This is correlated with a disorganization of the timing of granule cell proliferation and differentiation in the EGL. Many differentiated granule cells migrate inside the cerebellum and keep proliferating. These results reveal that Plexin-B2 controls the balance between proliferation and differentiation in granule cells.
Assuntos
Cerebelo/citologia , Cerebelo/crescimento & desenvolvimento , Proteínas do Tecido Nervoso/fisiologia , Receptores de Superfície Celular/fisiologia , Animais , Diferenciação Celular/genética , Diferenciação Celular/fisiologia , Proliferação de Células , Cerebelo/metabolismo , Feminino , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Mutação , Proteínas do Tecido Nervoso/deficiência , Proteínas do Tecido Nervoso/genética , Receptores de Superfície Celular/deficiência , Receptores de Superfície Celular/genéticaRESUMO
The acquisition of the dynamic balance between excitation and inhibition in developing Purkinje cells, necessary for their proper function, is analyzed. Newborn (P0) mouse cerebellum contains glutamatergic (VGLUT2-IR) and gamma-aminobutyric acid (GABA)-ergic (VIAAT-IR) axons. The former prevail and belong to climbing fibers, whereas the latter neither colabel with calbindin-expressing fibers nor belong to axons of the cortical GABAergic interneurons. During the first postnatal week, VIAAT-IR axons in the Purkinje cell neighborhood remains very low, and the first synapses with basket fibers are formed at P7, when climbing fibers have already established dense pericellular nets. The descending basket fibers reach the Purkinje cell axon initial segment by P9, immediately establishing axoaxonic synapses. The pinceaux appear as primitive vortex-like arrangements by P12, and by P20 interbasket fiber septate-like junctions, typical of fully mature pinceaux, are still missing. The climbing fiber's somatodendritic translocation occurs later than expected, after the regression of the multiple innervation, and follows the ascending collaterals of the basket axons, which are apparently the optimal substrate for the proper subcellular targeting of the climbing fibers. These results emphasize that chemical transmission in the axon initial segment precedes the electrical inhibition generated by field effects. In addition, GABAergic Purkinje cells, as opposed to glutamatergic projection neurons in other cortical structures, do not begin to receive their excitation to inhibition balance until the end of the first postnatal week, despite the early presence of potentially functional GABAergic axons that possess the required vesicular transport system.
Assuntos
Dendritos/fisiologia , Ácido Glutâmico/metabolismo , Fibras Nervosas/fisiologia , Células de Purkinje/citologia , Células de Purkinje/metabolismo , Ácido gama-Aminobutírico/metabolismo , Animais , Animais Recém-Nascidos , Cerebelo/citologia , Cerebelo/crescimento & desenvolvimento , Dendritos/ultraestrutura , Feminino , Regulação da Expressão Gênica no Desenvolvimento/fisiologia , Glutamato Descarboxilase/metabolismo , Proteínas de Fluorescência Verde/metabolismo , Camundongos , Camundongos Endogâmicos ICR , Camundongos Transgênicos , Microscopia Eletrônica de Transmissão/métodos , Fibras Nervosas/ultraestrutura , Fator de Transcrição PAX2/metabolismo , Parvalbuminas/metabolismo , Gravidez , Sinapses/fisiologia , Proteína Vesicular 2 de Transporte de Glutamato/metabolismo , Proteínas Vesiculares de Transporte de Aminoácidos Inibidores/metabolismoRESUMO
The modern age in the study of the cerebellum started 120 years ago when Cajal published his first paper with Golgi-impregnated material. In this publication, he selected the cerebellum to initiate his gigantic work aimed at unraveling the complexity of the CNS organization. It was not by chance that he selected the cerebellum but because of the occurrence of specific types of fibers, particularly climbing and mossy afferents and basket fibers. The peculiarity of these fibers offered Cajal one of the clearest situations to envision his "neuron doctrine", which proposes that between the nerve cell processes there is no continuity, only contiguity. In 4 years of intense investigation, Cajal was able to untangle the whole cerebellar circuit, providing the roots of our present knowledge on cerebellar organization. This knowledge has greatly expanded in the last 40 years mainly because the application of new techniques, such as electron microscopy, axonal connection tracing techniques based upon axoplasmic transports, and especially modern immunohistochemical and in situ hybridization techniques allowing the correlation of the chemical constituents of the cells with their structural counterparts, as a valuable approach to better appraise function and organization of the cerebellum. These post-Cajal discoveries are briefly discussed to conclude that, even though we are still far from a complete understanding of its function, new important concepts have been developed, for instance that through its connections with the prefrontal cortex, the cerebellum does not only contribute to the planning and execution of the movement, but that has access also to higher cognitive functions.
Assuntos
Cerebelo/fisiologia , Anatomia/história , Sistema Nervoso Central/anatomia & histologia , Sistema Nervoso Central/fisiologia , Cerebelo/anatomia & histologia , História do Século XIX , História do Século XX , Humanos , Neurônios/fisiologia , Fisiologia/históriaRESUMO
Recent advances in molecular biology have drastically changed our vision on the development of the nervous system, the cerebellum in particular. After a classical descriptive period, we are now in a modern mechanistic epoch as we begin to answer crucial questions in our quest to understand the mechanisms underlying the emergence of brain complexity. This review begins with an analysis of the role of the "isthmic organizer" in the induction and specification of the cerebellar territory and progresses through cerebellar development to the formation of cerebellar maps. It gathers information about the control of the proliferation of granule cell precursors by Purkinje cells and the role of Shh/Gli-patched signaling. The migratory routes for cerebellar and precerebellar neurons, together with the long-range and short-range cues guiding gliophilic and, particularly, neurophilic migrations, are also discussed. Because these cues are similar to those involved in axon guidance, both processes are under the same molecular constraints. Finally, using primarily the olivocerebellar projection as a model, the cellular and molecular mechanisms involved in the formation of cerebellar maps are discussed. During embryonic development, Purkinje cells in the cerebellum and neurons in the inferior olive follow a simultaneous, but independent, process of intrinsic parcellation, giving rise to subsets of biochemically different cortical compartments. The occurrence of positional information shared between olivary axons and their postsynaptic targets, the Purkinje cells, provides a molecular code for the formation of coarse-grained maps. Activity-dependent mechanisms are required for the transition from crude to fine-grained maps. This important refinement, which confers ultimate specificity to the maps, is under the regulation of parallel fiber-Purkinje cell synaptic activity.
Assuntos
Fenômenos Fisiológicos Celulares , Cerebelo/fisiologia , Regulação da Expressão Gênica no Desenvolvimento/fisiologia , Neurônios/fisiologia , Animais , Mapeamento Encefálico , Cerebelo/citologia , Cerebelo/embriologia , Humanos , Biologia Molecular/métodos , Fibras Nervosas/fisiologia , Proteínas do Tecido Nervoso/genética , Proteínas do Tecido Nervoso/fisiologia , Neurônios/citologia , Sinapses/fisiologiaRESUMO
Axonal regeneration in the mammalian CNS is a property of immature neurons that is lost during development. Using organotypic culture of cerebellum, we have shown that in vitro Purkinje cells lose their regenerative capacity in parallel with the process of myelination. We have investigated whether myelination is involved in the age-dependent loss of regeneration of these neurons. By applying a high dose of bromodeoxyuridine in the culture medium of newborn cerebellar slices during the first 3 d in vitro, we have succeeded in obtaining cultures with oligodendrocyte depletion, together with a lack of ameboid microglia and enhancement of Purkinje cell survival. These cultures, after 14 d in vitro, are completely devoid of myelin. We have compared the ability of Purkinje cells to regenerate their axons in the presence or absence of myelin. Purkinje cells in cerebellar explants taken at birth, treated with bromodeoxyuridine and axotomized after 7 d in vitro, survive better than similar neurons in untreated cultures. However, despite the lack of myelin and the enhanced survival, Purkinje cells do not regenerate, whereas they do regenerate when the axotomy is done at postnatal day 0. Thus, the Purkinje cell developmental switch from axonal regeneration to lack of regeneration does not appear to be regulated by myelin.
Assuntos
Axônios/fisiologia , Bainha de Mielina/metabolismo , Regeneração Nervosa/fisiologia , Células de Purkinje/fisiologia , Fatores Etários , Animais , Animais Recém-Nascidos , Astrócitos/efeitos dos fármacos , Axônios/efeitos dos fármacos , Axotomia , Bromodesoxiuridina/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Cerebelo/citologia , Regulação da Expressão Gênica no Desenvolvimento/fisiologia , Técnicas In Vitro , Camundongos , Camundongos Knockout , Microglia/efeitos dos fármacos , Bainha de Mielina/efeitos dos fármacos , Regeneração Nervosa/efeitos dos fármacos , Oligodendroglia/efeitos dos fármacos , Células de Purkinje/citologia , Células de Purkinje/efeitos dos fármacos , Fatores de TempoRESUMO
In organotypic cultures, mouse Purkinje cells regenerate their axons from embryonic day 18 (E18) to postnatal day 0 (P0), die of apoptosis between P1 and P7, and survive but do not regenerate at P10. This particular behavior of Purkinje cells did not allow us to find out when the developmental switch between regeneration and lack of regeneration occurs. This work was undertaken to suppress Purkinje cell apoptosis and to investigate whether the same molecules that prevent apoptosis could also influence axonal growth, regeneration, or both. We show that brain-derived neurotrophic factor, neurotrophin 3, and insulin-like growth factor I have marginal effects on P3 Purkinje cell death. The use of Gö6976 [a protein kinase C (PKC) inhibitor] or a transgenic mouse line, in which a pseudosubstrate PKC inhibitor has been specifically targeted to Purkinje cells, prevents the massive Purkinje cell death in P3 organotypic cultures. In addition, Gö6976 promotes axotomized Purkinje cell survival up to P7. Thus, the inhibition of PKC activity is able to prevent Purkinje cell apoptosis in organotypic cultures. Furthermore, Gö6976 increases the outgrowth of dendrites and axon collateralization, as shown after gene gun enhanced green fluorescent protein transfection. In contrast, PKC inhibitors do not influence the axonal regenerative capability of Purkinje cell during development; the latter decreases between E18 and P7 after the same time course in control and Gö6976-treated slices. Thus, because inhibition of PKC prevents Purkinje cell death but does not affect axonal regeneration, these two events (cell death and axonal regeneration) seem to be differentially regulated.
Assuntos
Apoptose/efeitos dos fármacos , Axônios/efeitos dos fármacos , Inibidores Enzimáticos/farmacologia , Regeneração Nervosa/efeitos dos fármacos , Proteína Quinase C/antagonistas & inibidores , Células de Purkinje/efeitos dos fármacos , Animais , Axônios/fisiologia , Axotomia , Fator Neurotrófico Derivado do Encéfalo/farmacologia , Carbazóis/farmacologia , Diferenciação Celular/fisiologia , Sobrevivência Celular/efeitos dos fármacos , Cerebelo/citologia , Cerebelo/embriologia , Dendritos/efeitos dos fármacos , Regulação da Expressão Gênica no Desenvolvimento , Técnicas In Vitro , Indóis/farmacologia , Fator de Crescimento Insulin-Like I/farmacologia , Camundongos , Camundongos Transgênicos , Regeneração Nervosa/fisiologia , Neurotrofina 3/farmacologia , Proteína Quinase C/biossíntese , Células de Purkinje/citologia , Células de Purkinje/metabolismo , Fatores de TempoRESUMO
The subventricular zone (SVZ) contains undifferentiated cells, which proliferate and generate olfactory bulb (OB) interneurons. Throughout life, these cells leave the SVZ and migrate along the rostral migratory stream (RMS) to the OB where they differentiate. In vitro, the septum and the choroid plexus (CP) secrete repulsive factors that could orient the migration of OB precursors. Slit1 and Slit2, two known chemorepellents for developing axons, can mimic this effect. We show here that the Slit receptors Robo2 and Robo3/Rig-1 are expressed in the SVZ and the RMS and that Slit1 and Slit2 are still present in the adult septum. Using Slit1/2-deficient mice, we found that Slit1 and Slit2 are responsible for both the septum and the CP repulsive activity in vitro. In adult mice lacking Slit1, small chains of SVZ-derived cells migrate caudally into the corpus callosum, supporting a role for Slits in orienting the migration of SVZ cells. Surprisingly, in adult mice, Slit1 was also expressed by type A and type C cells in the SVZ and RMS, suggesting that Slit1 could act cell autonomously. This hypothesis was tested using cultures of SVZ explants or isolated neurospheres from Slit1-/- or Slit1+/- mice. In both types of cultures, the migration of SVZ cells was altered in the absence of Slit1. This suggests that the regulation of the migration of OB precursors by Slit proteins is complex and not limited to repulsion.