Human Dermal Fibroblast Viability and Adhesion on Cellulose Nanomaterial Coatings: Influence of Surface Characteristics.

Biodegradable and renewable materials, such as cellulose nanomaterials, have been studied as a replacement material for traditional plastics in the biomedical field. Furthermore, in chronic wound care, modern wound dressings, hydrogels, and active synthetic extracellular matrices promoting tissue regeneration are developed to guide cell growth and differentiation. Cells are guided not only by chemical cues but also through their interaction with the surrounding substrate and its physicochemical properties. Hence, the current work investigated plant-based cellulose nanomaterials and their surface characteristic effects on human dermal fibroblast (HDF) behavior. Four thin cellulose nanomaterial-based coatings produced from microfibrillar cellulose (MFC), cellulose nanocrystals (CNC), and two TEMPO-oxidized cellulose nanofibers (CNF) with different total surface charge were characterized, and HDF viability and adhesion were evaluated. The highest viability and most stable adhesion were on the anionic CNF coating with a surface charge of 1.14 mmol/g. On MFC and CNC coated surfaces, HDFs sedimented but were unable to anchor to the substrate, leading to low viability.

Influence of mineral coatings on fibroblast behaviour: The importance of coating formulation and experimental design.

Mineral coatings manipulate surface properties such as roughness, porosity, wettability and surface energy. Properties that are known to determine cell behaviour. Therefore, mineral coatings can potentially be used to manipulate cell fate. This paper studies mineral-cell interactions through coatings in a stacked cell culture platform. Minerals were chosen according to their influence on Human Dermal Fibroblasts (HDFs): calcium carbonate, calcium sulphates, and kaolin. Mineral coatings were formulated with the additives latex, sorbitol, polyvinyl alcohol (PVOH) and TEMPO-oxidised cellulose nanofibrils (CNF-T). The coatings were placed as a bottom or top of the device, for a direct or indirect interaction with HDFs, respectively. Cells were seeded, in various densities, to the bottom of the device; and cell density and confluency were monitored in time. Overall, results show that the coating interaction is influenced at first by the cell seeding density. Scarce cell seeding density limits adaptability to the new environment, while an abundant one encourages confluency in time. In between those densities, coating formulation plays the next major role. Calcium carbonate promoted HDFs growth the most as expected, but the response to the rest of minerals depended on the coating additive. CNF-T encouraged proliferation even for kaolin, a mineral with long-term toxicity to HDFs, while PVOH induced a detrimental effect on HDF growth regardless of the mineral. At last, the placement of the coated layer provided insights on the contact-dependency of each response. This study highlights the importance of the experimental design, including coating formulation, when investigating cellular response to biomaterials.

Stacking up: a new approach for cell culture studies.

Traditional cell culture relies mostly on flat plastic surfaces, such as Petri dishes and multiwell plates. These commercial surfaces provide limited flexibility for experimental design. In contrast, cell biology increasingly demands surface customisation, functionalisation, and cell monitoring in order to obtain data that is relevant in vivo. The development of research areas such as microfluidics and electrochemical detection methods greatly promoted the customised design of cell culture platforms. However, the challenges for mass production and material limitations prevent their widespread usage and commercialisation. This article presents a new cell culture platform based on stacks of a transparent flexible printable substrate. The arrangement introduces multi-layered stacks for possible manipulation and access to the cells. The platform is highly compatible with current technologies, such as colorimetric imaging and fluorescence microscopy. In addition, it can potentially integrate, e.g., biomaterials, patterning, microfluidics, electrochemical detection and other techniques to influence, monitor, and assess cell behaviour in a multitude of different settings. More importantly, the platform is a low-cost alternative customisable through functional printing and coating technologies. The device shown in this manuscript represents a prototype for more sophisticated variations that will expand the relevance of in vitro studies in cell biology.

The influence of mineral particles on fibroblast behaviour: A comparative study.

Minerals are versatile tools utilised to modify and control the physical-chemical and functional properties of substrates. Those properties include ones directing cell fate; thus, minerals can potentially provide a direct and inexpensive method to manipulate cell behaviour. This paper shows how different minerals influence human dermal fibroblast behaviour depending on their properties. Different calcium carbonates, calcium sulphates, silica, silicates, and titanium dioxide were characterised using TEM, ATR-FTIR, and zeta potential measurements. Mineral-cell interactions were analysed through MTT assay, LDH assay, calcein AM staining, live cell imaging, immunofluorescence staining, western blot, and extra/intracellular calcium measurements. Results show that the interaction of the fibroblasts with the minerals was governed by a shared period of adaptation, followed by increased proliferation, growth inhibition, or increased toxicity. Properties such as size, ion release and chemical composition had a direct influence on the cells leading to cell agglomeration, morphological changes, and the possible formation of protein-mineral complexes. In addition, zeta potential and FTIR measurements of the minerals showed adsorption of the cell culture media onto the particles. This article provides fundamental insight into the mineral-fibroblast interactions, and makes it possible to arrange the minerals according to the time-dependent cellular response.