Color category and inter-item interaction influence color working memory codependently.

Our brains do not always encode visual information in a veridical way. Visual working memory (WM) for features such as color can be biased. WM bias comes from several sources. Category priors can lead to WM bias. For example, color WM is biased toward or away from category prototypes. In addition to category knowledge, contextual factors can induce and modulate WM bias; however, these biases of different sources have usually been investigated independently with different tasks. The present study sought to explore how color WM is influenced by both color category and concurrent distractor. Specifically, we asked participants to retain two color items in WM to investigate how the WM representation of the target color is biased by learned category knowledge and contextual inter-item interactions. Our study found that the WM representation of the target color is biased toward or away from the category prototypes and away from the distractor color that is simultaneously held in WM, indicating that both color category and concurrent distractor bias color WM. More importantly, the weight of these two biases depends on the specific color category, suggesting that category priors and inter-item interaction biases are not simply additive but flexible. Furthermore, we revealed that both types of biases arise from perceptual processes.

A genuine interindividual variability in number and anatomical localization of face-selective regions in the human brain.

Neuroimaging studies have reported regions with more neural activation to face than nonface stimuli in the human occipitotemporal cortex for three decades. Here we used a highly sensitive and reliable frequency-tagging functional magnetic resonance imaging paradigm measuring high-level face-selective neural activity to assess interindividual variability in the localization and number of face-selective clusters. Although the majority of these clusters are located in the same cortical gyri and sulci across 25 adult brains, a volume-based analysis of unsmoothed data reveals a large amount of interindividual variability in their spatial distribution and number, particularly in the ventral occipitotemporal cortex. In contrast to the widely held assumption, these face-selective clusters cannot be objectively related on a one-to-one basis across individual brains, do not correspond to a single cytoarchitectonic region, and are not clearly demarcated by estimated posteroanterior cytoarchitectonic borders. Interindividual variability in localization and number of cortical face-selective clusters does not appear to be due to the measurement noise but seems to be genuine, casting doubt on definite labeling and interindividual correspondence of face-selective "areas" and questioning their a priori definition based on cytoarchitectony or probabilistic atlases of independent datasets. These observations challenge conventional models of human face recognition based on a fixed number of discrete neurofunctional information processing stages.

Categorical encoding of moving colors during location tracking.

Categorical perception (CP) describes our tendency to perceive the visual world in a categorical manner, suggesting that high-level cognition may affect perception. While most studies are conducted in static visual scenes, Sun and colleagues found CP effects of color in multiple object tracking (MOT). This study used functional magnetic resonance imaging to investigate the neural mechanism behind the categorical effects of color in MOT. Categorical effects were associated with activities in a broad range of brain regions, including both the ventral (V4, middle temporal gyrus) and dorsal pathways (MT + /V5, inferior parietal lobule) of feature processing, as well as frontal regions (middle frontal gyrus, medial superior frontal gyrus). We proposed that these regions are hierarchically organized and responsible for distinct functions. The color-selective V4 encodes color categories, making cross-category colors more discriminable than within-category colors. Meanwhile, the language and/or semantic regions encode the verbal information of the colors. Both visual and nonvisual codes of color categories then modulate the activities of motion-sensitive MT + areas and frontal areas responsible for attentional processes.

Language Modulates Categorical Effects of Moving Color Objects.

Categorical perception (CP) of color claims that colors from different linguistic categories are discriminated more easily than those from the same category, suggesting that language may interact with visual perception. However, controversy remains regarding whether CP effects derive from language. Recently, CP effects were found in a dynamic paradigm named multiple object tracking (MOT). Here, we investigated whether this kind of CP is dependent on online use of language. We found that the CP effects are reduced by verbal interference when the participants were required to memorize color words during tracking (Experiment 2) but not when the interference stimuli were digits (Experiment 1). Our study suggested that the CP effects in tracking depend partly on online use of language and that the difficulty of verbal interference tasks influences the capability of disrupting CP.

Tracking within-category colors is easier: Color categories modulate location processing in a dynamic visual task.

The categorical perception (CP) of color describes the phenomenon that colors across categories (e.g., blue and green) are more discriminable than within-category colors (e.g., green) even when the perceptual distance is controlled. While most studies are conducted in a static visual scene, the current study investigated the effect of color categories when tracking multiple colored objects within two experiments. The targets or distractors were either from the same color category or from two different categories, and the perceptual distance was controlled across all conditions. We found that location tracking was facilitated when the targets or the distractors shared the same color category in the tracking task, which required location tracking and color memorization at the same time, compared with when they were from two different categories (Experiment 1). By contrast, when location tracking was the sole demand and no explicit color processing was required, the target CP effects, but not the distractor CP effects, persisted (Experiment 2). We conclude that color categories can dynamically modulate preattention visual processing when color working memory is involved, but that attention is necessary to produce CP effects when color is irrelevant to the task.

The applicability of eye-controlled highlighting to the field of visual searching.

OBJECTIVE: With the increasing amount of information presented on current human-computer interfaces, eye-controlled highlighting has been proposed, as a new display technique, to optimise users' task performances. However, it is unknown to what extent the eye-controlled highlighting display facilitates visual search performance. The current study examined the facilitative effect of eye-controlled highlighting display technique on visual search with two major attributes of visual stimuli: stimulus type and the visual similarity between targets and distractors. METHOD: In Experiment 1, we used digits and Chinese words as materials to explore the generalisation of the facilitative effect of the eye-controlled highlighting. In Experiment 2, we used Chinese words to examine the effect of target-distractor similarity on the facilitation of eye-controlled highlighting display. RESULTS: The eye-controlling highlighting display improved visual search performance when words were used as searching target and when the target-distractor similarity was high. No facilitative effect was found when digits were used as searching target or target-distractor similarity was low. CONCLUSIONS: The effectiveness of the eye-controlled highlighting on a visual task was influenced by both stimulus type and target-distractor similarity. These findings provided guidelines for modern interface design with eye-based displays implemented.

Rapid color categorization revealed by frequency-tagging-based EEG.

There has been much debate on whether color categories affect how we perceive color. Recent theories have put emphasis on the role of top-down influence on color perception that the original continuous color space in the visual cortex may be transformed into categorical encoding due to top-down modulation. To test the influence of color categories on color perception, we adopted an RSVP paradigm, where color stimuli were presented at a fast speed of 100 ms per stimulus and were forward and backward masked by the preceding and following stimuli. Moreover, no explicit color naming or categorization was required. In theory, backward masking with such a short interval in a passive viewing task should constrain top-down influence from higher-level brain areas. To measure any potentially subtle differences in brain response elicited by different color categories, we embedded a sensitive frequency-tagging-based EEG paradigm within the RSVP stimuli stream where the oddball color stimuli were encoded with a different frequency from the base color stimuli. We showed that EEG responses to cross-category oddball colors at the frequency where the oddball stimuli were presented was significantly larger than the responses to within-category oddball colors. Our study suggested that the visual cortex can automatically and implicitly encode color categories when color stimuli are presented rapidly.

Repulsion bias is insensitive to spatial attention, yet expands during active working memory maintenance.

Our brain sometimes represents visual information in a biased manner. Multiple visual features presented simultaneously or sequentially may interact with each other when we perceive them or maintain them in visual working memory (WM), giving rise to report bias. How goal-directed attention influences target representation is not fully understood, especially concerning whether attention towards distractors modulates report bias for the target. Our study investigated the WM biases of the target when it is concurrent with (1) one attended distractor only, (2) one unattended distractor only, and (3) both kinds of distractors during perception. It was found that the target WM is reported as being repelled away from concurrent distractors, attended or unattended, suggesting attention is not necessary for the occurrence of repulsion bias during perception. Furthermore, goal-directed attention towards the distractors modulates the strength of interitem interaction, and the repulsion bias was found to be stronger when attention was directed toward the distractor than when it was not. However, the exaggerated repulsion associated with the attended distractor is likely due to increased relevance to the memory task and (or) WM load instead of spatial attention. In contrast, spatial attention towards the distractor increases the chances of misreporting the distractor for the target.

Other ethnicity effects in ensemble coding of facial expressions.

Cultural difference in ensemble emotion perception is an important research question, providing insights into the complexity of human cognition and social interaction. Here, we conducted two experiments to investigate how emotion perception would be affected by other ethnicity effects and ensemble coding. In Experiment 1, two groups of Asian and Caucasian participants were tasked with assessing the average emotion of faces from their ethnic group, other ethnic group, and mixed ethnicity groups. Results revealed that participants exhibited relatively accurate yet amplified emotion perception of their group faces, with a tendency to overestimate the weight of the faces from the other ethnic group. In Experiment 2, Asian participants were instructed to discern the emotion of a target face surrounded by faces from Caucasian and Asian faces. Results corroborated earlier findings, indicating that while participants accurately perceived emotions in faces of their ethnicity, their perception of Caucasian faces was noticeably influenced by the presence of surrounding Asian faces. These findings collectively support the notion that the other ethnicity effect stems from differential emotional amplification inherent in ensemble coding of emotion perception.

Manganese inhibits HBV transcription and promotes HBsAg degradation at non-toxic levels.

Chronic hepatitis B virus (HBV) infection continues to pose a significant global health challenge. However, therapeutic measures for a cure are lacking in clinical practice. Manganese, an essential trace element, has garnered attention due to its potential to activate innate immune pathways and its significant role in antiviral and antitumor immunity. Yet, the specific impact of manganese on chronic hepatitis B has been largely unexplored. Our research reveals that manganese substantially inhibits HBV replication in hepatocellular carcinoma cells at non-toxic levels. This suppression occurs independently of well-known anti-HBV innate immune pathways, such as the cGAS-STING pathway. Mechanistically, manganese decreases HBV transcription by diminishing the levels of liver-specific transcription factors. Furthermore, it activates the mTOR pathway, enhancing HBsAg ubiquitination through the upregulation of the ubiquitin ligase ß-TrCP and increasing proteasome activity via the augmentation of its subunits, leading to a ubiquitin-dependent degradation of HBsAg. Significantly, our study also uncovers a notable clinical correlation between manganese levels and chronic hepatitis B infection. These findings position manganese as a critical element in diminishing HBV replication, offering a new direction in the management of chronic hepatitis B.

Functional Differentiation of BnVTE4 Gene Homologous Copies in α-Tocopherol Biosynthesis Revealed by CRISPR/Cas9 Editing.

Tocopherols are essential nutrients for human health known as vitamin E. Vitamin E deficiency can have a profound effect on human health, including the central nervous system and cardiovascular and immune protection. Multiple enzymatic steps are involved in the conversion between different forms of tocopherols. Among them, γ-tocopherol methyltransferase encoded by gene VTE4 catalyzes the conversion of γ- to α-tocopherol or δ- to ß-tocopherol isoforms. However, the gene copies and their functional contribution of VTE4 homologs in Brassica napus were not elucidated. To this end, different mutation combinations of four putative BnVTE4 homologous copies were generated by using CRISPR/Cas9 genome editing technology. Editing of those BnVTE4 homologs led to a significant change of the α-tocopherol content and the ratio between α- and γ-tocopherol compared with wide-type control. Analysis of the different combinations of BnVTE4-edited homologs revealed that the contribution of the BnVTE4 individual gene displayed obvious functional differentiation in α-tocopherol biosynthesis. Their contribution could be in order of VTE4.C02-2 (BnaC02G0331100ZS) > VTE4.A02-1 (BnaA02G0247300ZS) > VTE4.A02-2 (BnaA02G0154300ZS). Moreover, the VTE4.A02-1 and VTE4.A02-2 copies might have severe functional redundancies in α-tocopherol biosynthesis. Overall, this study systemically studied the different effects of BnVTE4 homologs, which provided a theoretical basis for breeding high α-tocopherol content oilseed rape.

Neural Hierarchy of Color Categorization: From Prototype Encoding to Boundary Encoding.

A long-standing debate exists on how our brain assigns the fine-grained perceptual representation of color into discrete color categories. Recent functional magnetic resonance imaging (fMRI) studies have identified several regions as the candidate loci of color categorization, including the visual cortex, language-related areas, and non-language-related frontal regions, but the evidence is mixed. Distinct from most studies that emphasized the representational differences between color categories, the current study focused on the variability among members within a category (e.g., category prototypes and boundaries) to reveal category encoding in the brain. We compared and modeled brain activities evoked by color stimuli with varying distances from the category boundary in an active categorization task. The frontal areas, including the inferior and middle frontal gyri, medial superior frontal cortices, and insular cortices, showed larger responses for colors near the category boundary than those far from the boundary. In addition, the visual cortex encodes both within-category variability and cross-category differences. The left V1 in the calcarine showed greater responses to colors at the category center than to those far from the boundary, and the bilateral V4 showed enhanced responses for colors at the category center as well as colors around the boundary. The additional representational similarity analyses (RSA) revealed that the bilateral insulae and V4a carried information about cross-category differences, as cross-category colors exhibited larger dissimilarities in brain patterns than within-category colors. Our study suggested a hierarchically organized network in the human brain during active color categorization, with frontal (both lateral and medial) areas supporting domain-general decisional processes and the visual cortex encoding category structure and differences, likely due to top-down modulation.

The Application of Complexity Analysis in Brain Blood-Oxygen Signal.

One of the daunting features of the brain is its physiology complexity, which arises from the interaction of numerous neuronal circuits that operate over a wide range of temporal and spatial scales, enabling the brain to adapt to the constantly changing environment and to perform various cognitive functions. As a reflection of the complexity of brain physiology, the complexity of brain blood-oxygen signal has been frequently studied in recent years. This paper reviews previous literature regarding the following three aspects: (1) whether the complexity of the brain blood-oxygen signal can serve as a reliable biomarker for distinguishing different patient populations; (2) which is the best algorithm for complexity measure? And (3) how to select the optimal parameters for complexity measures. We then discuss future directions for blood-oxygen signal complexity analysis, including improving complexity measurement based on the characteristics of both spatial patterns of brain blood-oxygen signal and latency of complexity itself. In conclusion, the current review helps to better understand complexity analysis in brain blood-oxygen signal analysis and provide useful information for future studies.

Multiple representations in visual working memory simultaneously guide attention: The type of memory-matching representation matters.

Whether multiple visual working memory (VWM) representations can simultaneously become active templates to guide attention is controversial. The single-item-template hypothesis argues that only one VWM representation can be active at a time, whereas the multiple-item-template hypothesis argues that multiple VWM templates can simultaneously guide attention. The present study examined the two hypotheses in three (out of four) experiments, using three different types of memory objects: Experiment 1: shapes; Experiment 2: colors; and Experiment 3: colored shapes. Participants were required to hold one (memory-1) or two objects (memory-2) in VWM while performing a tilted line search task. Zero (match-0), one (match-1), or two (match-2) memory stimuli reappeared as distractors in the search array. Guidance effects were found for each type of memory stimuli. More importantly, the guidance effect for memory-2/match-2 trials was significantly larger than that for memory-2/match-1 and memory-1/match-1 trials when holding two colors or two colored shapes in VWM, which is in line with the multiple-item-template hypothesis. However, the pattern of simultaneous guidance effect is not perfectly found for two memory shapes, which may indicate that a reliable simultaneous guidance effect from two representations in VWM can be observed only when the memory-matching stimuli is more effective in guiding attention. Experiment 4 directly compared the guidance effect induced by feature-based matches (partial matching) with the guidance effect induced object-based matches (complete matching) in memory-set-size 2. Reliable guidance effects in match-1 and match-2 trials for object-based matches but not for feature-based matches confirmed the crucial role of the type of memory-matching stimuli in guiding attention.

CRISPR/Cas9-Mediated Multiplex Genome Editing of JAGGED Gene in Brassica napus L.

Pod shattering resistance is an essential component to achieving a high yield, which is a substantial objective in polyploid rapeseed cultivation. Previous studies have suggested that the Arabidopsis JAGGED (JAG) gene is a key factor implicated in the regulatory web of dehiscence fruit. However, its role in controlling pod shattering resistance in oilseed rape is still unknown. In this study, multiplex genome editing was carried out by the CRISPR/Cas9 system on five homoeologs (BnJAG.A02, BnJAG.C02, BnJAG.C06, BnJAG.A07, and BnJAG.A08) of the JAG gene. Knockout mutagenesis of all homoeologs drastically affected the development of the lateral organs in organizing pod shape and size. The cylindrical body of the pod comprised a number of undifferentiated cells like a callus, without distinctive valves, replum, septum, and valve margins. Pseudoseeds were produced, which were divided into two halves with an incomplete layer of cells (probably septum) that separated the undifferentiated cells. These mutants were not capable of generating any productive seeds for further generations. However, one mutant line was identified in which only a BnJAG.A08-NUB-Like paralog of the JAG gene was mutated. Knockout mutagenesis in BnJAG.A08-NUB gene caused significant changes in the pod dehiscence zone. The replum region of the mutant was increased to a great extent, resulting in enlarged cell size, bumpy fruit, and reduced length compared with the wild type. A higher replum-valve joint area may have increased the resistance to pod shattering by ~2-fold in JAG mutants compared with wild type. Our results offer a basis for understanding variations in Brassica napus fruit by mutating JAG genes and providing a way forward for other Brassicaceae species.

Hue distinctiveness overrides category in determining performance in multiple object tracking.

The visual distinctiveness between targets and distractors can significantly facilitate performance in multiple object tracking (MOT), in which color is a feature that has been commonly used. However, the processing of color can be more than "visual." Color is continuous in chromaticity, while it is commonly grouped into discrete categories (e.g., red, green). Evidence from color perception suggested that color categories may have a unique role in visual tasks independent of its chromatic appearance. Previous MOT studies have not examined the effect of chromatic and categorical distinctiveness on tracking separately. The current study aimed to reveal how chromatic (hue) and categorical distinctiveness of color between the targets and distractors affects tracking performance. With four experiments, we showed that tracking performance was largely facilitated by the increasing hue distance between the target set and the distractor set, suggesting that perceptual grouping was formed based on hue distinctiveness to aid tracking. However, we found no color categorical effect, because tracking performance was not significantly different when the targets and distractors were from the same or different categories. It was concluded that the chromatic distinctiveness of color overrides category in determining tracking performance, suggesting a dominant role of perceptual feature in MOT.

Conflict Tasks of Different Types Divergently Affect the Attentional Processing of Gaze and Arrow.

The present study explored the attentional processing mechanisms of gaze and arrow cues in two different types of conflict tasks. In Experiment 1, participants performed a flanker task in which gaze and arrow cues were presented as central targets or bilateral distractors. The congruency between the direction of the target and the distractors was manipulated. Results showed that arrow distractors greatly interfered with the attentional processing of gaze, while the processing of arrow direction was immune to conflict from gaze distractors. Using a spatial compatibility task, Experiment 2 explored the conflict effects exerted on gaze and arrow processing by their relative spatial locations. When the direction of the arrow was in conflict with its spatial layout on screen, response times were slowed; however, the encoding of gaze was unaffected by spatial location. In general, processing to an arrow cue is less influenced by bilateral gaze cues but is affected by irrelevant spatial information, while processing to a gaze cue is greatly disturbed by bilateral arrows but is unaffected by irrelevant spatial information. Different effects on gaze and arrow cues by different types of conflicts may reflect two relatively distinct specific modes of the attentional process.