RESUMO
BACKGROUND: Treatment of thin endometrium with granular leukocyte-colony stimulating factor (G-CSF) remains controversial. AIM: To investigate the effect of G-CSF on the outcome of frozen embryo transfer in patients with thin endometrium. METHODS: A retrospective propensity score matching (PSM) study was performed to assess patients administered frozen embryo transfer at the Reproductive Medicine Center of the Affiliated Drum Tower Hospital of Nanjing University Medical School, in 2012-2018. The patients were divided into G-CSF intrauterine perfusion (G-CSF) and non-G-CSF groups, and clinical pregnancy, implantation, ectopic pregnancy, and early abortion rates between the two groups were compared. RESULTS: Before PSM, 372 cycles were enrolled, including 242 and 130 cycles in the G-CSF and non-G-CSF groups, respectively. Age (34.23 ± 5.76 vs 32.99 ± 5.59 years; P = 0.047) and the blastula/cleavage stage embryo ratio (0.68 vs 0.37; P = 0.011) were significantly elevated in the G-CSF group compared with the non-G-CSF group; however, clinical pregnancy (46.28% vs 51.54%; P = 0.371) and embryo implantation (35.21% vs 35.65%; P = 0.910) rates were similar in both groups. After PSM by age and blastula/cleavage stage embryo ratio, 244 cycles were included (122 cases each in the G-CSF and non-G-CSF groups). The clinical pregnancy (50.82 % vs 48.36%; P = 0.701) and embryo implantation (37.38% vs 34.11%; P = 0.480) remained similar in both groups. CONCLUSION: Intrauterine infusion of G-CSF does not improve the clinical outcome of frozen embryo transfer in patients with thin endometrium.
RESUMO
A field experiment with five treatments, control (CK, no fertilizer), conventional fertilization (U), double-effect inhibitor synergistic urea (DU), coated urea (CU) and slow/controlled release urea mixture (CDU), was conducted to investigate the effects of conventional fertilization (240 kg N·hm-2) and one-off application of different slow/controlled release fertilizers (180 kg N·hm-2) on the yield and quality of fresh maize, soil inorganic nitrogen (N), and ammonia (NH3) emissions. The results showed that the total amount of ammonia volatilization was the highest in treatment of conventional fertilization (U), with N topdressing being an important source of NH3 emission. Compared with U treatment, the NH3 volatilization in the DU, CU, and CDU treatments was reduced by 78%-81%. At harvest stage, the soil layer of 80-100 cm in the U treatment had the highest nitrate concentration (51.6 mg·kg-1), resulting in a high risk of N leaching. In contrast, the nitrate concentrations in the same soil layer in the slow/controlled release fertilizer treatments were much lower, reducing the risk of leaching. In comparison with U, three slow/controlled release fertilizer treatments with 25% lower N application did not decrease yield but increased seed Vc, soluble sugar and protein contents. The agronomic efficiency and economic benefit of DU treatment were the highest among three slow/controlled release fertilizers treatments. In conclusion, the application of new type of slow/controlled release fertilizer could improve the yield and quality of fresh maize, and significantly reduce the risk of ammonia loss and N leaching. Considering the higher cost of the polymer coated urea, the double-effect inhibitor urea has lower cost and is more convenient to make. It is therefore a better choice to fresh maize planting.